GREMLIN Database
GIDE - E3 Ubiquitin ligase
PFAM: PF12483 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 159 (148)
Sequences: 1847 (1543)
Seq/√Len: 126.8
META: 0.775

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
55_V90_I4.4441.00
99_V125_I3.3501.00
18_W85_R3.2551.00
31_Y38_R3.2341.00
98_Y130_E3.0821.00
101_G113_I2.9111.00
146_I150_V2.8401.00
143_L147_G2.7211.00
45_P48_A2.7151.00
129_S132_E2.6631.00
32_L97_L2.4021.00
53_E92_P2.3591.00
20_T23_S2.3091.00
33_E38_R2.2811.00
30_F45_P2.2761.00
139_S143_L2.1691.00
70_A73_G2.1271.00
148_G152_G2.0291.00
67_P71_L1.9421.00
102_E116_P1.8581.00
31_Y40_L1.8001.00
45_P125_I1.7521.00
54_T57_D1.7441.00
34_D37_G1.7211.00
140_S143_L1.6681.00
81_T84_Y1.6491.00
72_G76_G1.5561.00
91_L97_L1.5491.00
47_G115_K1.5081.00
39_V113_I1.4881.00
71_L75_S1.4331.00
70_A76_G1.4291.00
70_A74_S1.4071.00
99_V113_I1.3941.00
147_G150_V1.3861.00
95_D127_T1.3631.00
57_D87_T1.3591.00
40_L44_D1.3491.00
98_Y128_K1.3351.00
59_F83_G1.3221.00
39_V111_L1.3111.00
52_L127_T1.2981.00
142_R146_I1.2951.00
21_V84_Y1.2811.00
92_P95_D1.2801.00
145_L148_G1.2601.00
118_D121_P1.2491.00
138_G142_R1.2491.00
63_Y66_G1.2401.00
66_G78_R1.2341.00
66_G73_G1.2341.00
106_D111_L1.2240.99
147_G151_L1.2210.99
52_L89_E1.2030.99
140_S147_G1.1860.99
73_G78_R1.1780.99
16_S19_E1.1670.99
18_W116_P1.1290.99
91_L127_T1.1240.99
67_P75_S1.0830.99
66_G70_A1.0720.99
29_P44_D1.0630.99
72_G75_S1.0630.99
73_G77_R1.0620.99
7_R10_R1.0590.98
65_S74_S1.0570.98
143_L146_I1.0570.98
147_G154_A1.0410.98
69_G76_G1.0350.98
66_G74_S1.0310.98
74_S78_R1.0240.98
50_L152_G1.0140.98
30_F125_I1.0100.98
63_Y78_R1.0090.98
67_P74_S1.0050.98
7_R11_R1.0020.98
128_K132_E1.0010.98
12_T15_E0.9970.98
97_L127_T0.9710.97
9_G12_T0.9550.97
104_R111_L0.9550.97
104_R112_E0.9440.97
104_R114_A0.9380.97
64_Y70_A0.9340.97
32_L127_T0.9150.96
23_S94_G0.9080.96
145_L149_I0.9020.96
71_L76_G0.8970.96
40_L112_E0.8740.95
142_R148_G0.8700.95
53_E95_D0.8660.95
138_G141_A0.8590.95
75_S78_R0.8590.95
54_T89_E0.8570.95
13_R32_L0.8550.95
72_G77_R0.8550.95
73_G76_G0.8370.94
103_A113_I0.8220.93
106_D109_G0.8210.93
11_R15_E0.8120.93
135_S139_S0.8110.93
40_L45_P0.8080.93
146_I154_A0.8020.92
45_P49_E0.8010.92
102_E118_D0.8000.92
150_V154_A0.7920.92
97_L122_P0.7890.92
41_V99_V0.7880.92
68_D72_G0.7830.91
70_A75_S0.7780.91
67_P78_R0.7760.91
64_Y78_R0.7630.90
128_K136_S0.7570.90
52_L55_V0.7550.90
145_L152_G0.7500.90
111_L115_K0.7390.89
32_L113_I0.7370.89
34_D130_E0.7360.89
60_E150_V0.7330.89
67_P70_A0.7300.88
100_L133_L0.7290.88
111_L114_A0.7190.88
110_A114_A0.7170.87
146_I149_I0.7040.87
138_G149_I0.7010.86
128_K133_L0.7010.86
39_V98_Y0.6990.86
63_Y68_D0.6970.86
36_T141_A0.6970.86
48_A51_D0.6960.86
67_P73_G0.6920.86
90_I103_A0.6880.85
10_R14_T0.6830.85
13_R17_G0.6820.85
9_G13_R0.6740.84
44_D48_A0.6700.84
144_L149_I0.6660.83
52_L91_L0.6620.83
132_E155_G0.6570.83
109_G129_S0.6530.82
62_L67_P0.6510.82
126_S153_V0.6510.82
15_E19_E0.6500.82
111_L123_F0.6460.81
134_L138_G0.6450.81
40_L110_A0.6430.81
61_P64_Y0.6420.81
98_Y103_A0.6370.81
118_D142_R0.6370.81
67_P72_G0.6370.81
18_W24_G0.6370.81
148_G154_A0.6360.80
68_D71_L0.6340.80
13_R91_L0.6300.80
105_E110_A0.6300.80
52_L58_R0.6290.80
18_W21_V0.6240.79
142_R149_I0.6240.79
142_R152_G0.6210.79
60_E63_Y0.6200.79
111_L119_G0.6180.79
8_D13_R0.6160.78
139_S147_G0.6110.78
68_D77_R0.6100.78
94_G97_L0.6090.78
21_V94_G0.6080.77
99_V107_G0.6010.77
65_S68_D0.6000.76
148_G151_L0.5980.76
39_V44_D0.5920.76
69_G77_R0.5910.75
55_V92_P0.5910.75
144_L148_G0.5890.75
102_E117_K0.5840.75
140_S144_L0.5840.75
65_S75_S0.5830.74
60_E64_Y0.5800.74
142_R150_V0.5790.74
59_F82_K0.5760.74
153_V156_L0.5710.73
103_A115_K0.5700.73
32_L39_V0.5680.73
66_G71_L0.5630.72
65_S78_R0.5600.71
75_S79_E0.5580.71
51_D124_I0.5580.71
120_G136_S0.5550.71
95_D120_G0.5550.71
86_F103_A0.5540.71
30_F41_V0.5540.71
29_P40_L0.5530.71
31_Y110_A0.5500.70
98_Y123_F0.5470.70
72_G78_R0.5470.70
50_L93_P0.5470.70
57_D85_R0.5420.69
14_T18_W0.5400.69
77_R146_I0.5370.68
62_L66_G0.5360.68
143_L148_G0.5340.68
52_L56_F0.5340.68
14_T19_E0.5330.68
106_D110_A0.5320.68
19_E117_K0.5290.67
49_E156_L0.5280.67
34_D126_S0.5280.67
118_D141_A0.5270.67
10_R13_R0.5260.67
65_S70_A0.5210.66
69_G72_G0.5210.66
45_P51_D0.5200.66
68_D73_G0.5180.66
12_T41_V0.5160.65
41_V112_E0.5150.65
61_P74_S0.5140.65
103_A111_L0.5140.65
137_L141_A0.5120.65
69_G74_S0.5120.65
100_L112_E0.5080.64
106_D112_E0.5020.63
16_S83_G0.5010.63
137_L140_S0.5000.63
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3kdfD 2 0.6289 5.8 0.927 Contact Map
4wl1A 3 0 4.4 0.931 Contact Map
2jxxA 1 0.478 2.8 0.937 Contact Map
4joiA 1 0.7421 2.6 0.938 Contact Map
1i17A 1 0.2327 2.5 0.939 Contact Map
3aqlA 2 0.5472 2.4 0.94 Contact Map
2pi2A 1 0.6478 2.4 0.94 Contact Map
2lk9A 1 0.1069 2.3 0.94 Contact Map
1afoA 2 0.1824 2.1 0.942 Contact Map
3wfpA 2 0.478 2 0.942 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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