GREMLIN Database
DUF3661 - Vaculolar membrane protein
PFAM: PF12400 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 126 (125)
Sequences: 576 (435)
Seq/√Len: 38.9
META: 0.431

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
45_G48_P4.5531.00
1_C4_Y2.8701.00
19_L60_Y2.8431.00
115_L119_I2.7701.00
93_T96_N2.5951.00
101_L106_L2.5401.00
60_Y63_I2.4191.00
68_K90_L2.4181.00
53_W57_L2.3211.00
16_V112_M2.2100.99
102_V107_I2.1410.99
40_E43_N2.1110.99
60_Y64_L2.0060.99
5_F108_F1.9570.99
12_T112_M1.8680.98
19_L120_I1.7930.97
84_W102_V1.7420.97
42_G56_Q1.6230.95
18_I32_K1.5590.94
104_V109_P1.5360.94
60_Y120_I1.5260.94
14_L21_L1.5250.94
7_N86_G1.5240.94
69_L73_L1.5040.93
14_L40_E1.4850.93
7_N68_K1.4850.93
118_W122_S1.4800.93
109_P113_N1.4630.92
41_S82_L1.4590.92
120_I124_L1.3480.88
59_I123_F1.3440.88
112_M116_Q1.3360.88
102_V125_K1.3260.88
103_F108_F1.3200.87
8_F16_V1.2960.86
121_D125_K1.2820.86
25_L32_K1.2680.85
118_W124_L1.2630.85
23_L63_I1.2520.84
2_S90_L1.2480.84
76_L85_V1.2380.84
57_L61_L1.2380.84
47_P60_Y1.2070.82
29_L46_D1.1920.81
107_I111_I1.1730.80
6_L86_G1.1590.79
54_L57_L1.1550.79
43_N46_D1.1530.79
21_L24_R1.1500.79
2_S86_G1.1370.78
38_S90_L1.1330.78
70_I89_L1.1140.77
44_Y56_Q1.1020.76
27_T69_L1.0920.75
60_Y106_L1.0830.75
25_L54_L1.0750.74
121_D124_L1.0750.74
120_I123_F1.0730.74
22_L119_I1.0710.74
78_F81_P1.0580.73
4_Y121_D1.0360.71
68_K100_E1.0200.70
29_L33_T1.0190.70
68_K105_M1.0090.69
28_R32_K1.0060.69
65_L111_I1.0050.69
106_L110_L1.0040.69
46_D54_L1.0040.69
57_L60_Y0.9860.67
59_I114_A0.9850.67
59_I110_L0.9810.67
3_W36_P0.9750.67
1_C11_D0.9720.66
90_L100_E0.9690.66
53_W56_Q0.9670.66
2_S10_I0.9590.65
19_L124_L0.9230.62
32_K58_L0.9150.62
53_W105_M0.9100.61
15_G68_K0.9080.61
22_L63_I0.9060.61
66_L70_I0.9010.60
11_D15_G0.8920.60
104_V111_I0.8910.59
58_L124_L0.8820.59
16_V116_Q0.8810.59
33_T61_L0.8780.58
52_W97_P0.8740.58
40_E94_E0.8610.57
101_L105_M0.8460.56
60_Y101_L0.8390.55
68_K71_V0.8280.54
21_L111_I0.8240.54
51_K54_L0.8220.53
23_L67_M0.8140.53
62_L70_I0.8120.52
12_T16_V0.8100.52
4_Y15_G0.8060.52
30_A39_I0.7960.51
12_T116_Q0.7920.51
55_K116_Q0.7690.49
87_D93_T0.7620.48
53_W125_K0.7600.48
47_P100_E0.7580.48
20_Y83_A0.7550.47
21_L28_R0.7540.47
116_Q125_K0.7450.46
25_L51_K0.7400.46
42_G82_L0.7360.46
53_W123_F0.7350.46
114_A117_F0.7270.45
119_I123_F0.7270.45
85_V88_W0.7240.45
118_W121_D0.7220.44
33_T51_K0.7160.44
32_K66_L0.7150.44
11_D14_L0.7140.44
76_L79_P0.7140.44
49_S115_L0.7140.44
80_W85_V0.7140.44
80_W93_T0.7120.43
22_L62_L0.7120.43
53_W117_F0.6990.42
61_L123_F0.6990.42
62_L80_W0.6910.42
70_I74_L0.6900.42
118_W125_K0.6860.41
106_L124_L0.6810.41
18_I51_K0.6790.41
2_S5_F0.6780.40
31_S87_D0.6640.39
22_L66_L0.6610.39
8_F100_E0.6570.39
21_L102_V0.6520.38
21_L84_W0.6510.38
30_A82_L0.6480.38
106_L120_I0.6480.38
26_L41_S0.6440.38
14_L66_L0.6350.37
37_E55_K0.6350.37
36_P39_I0.6340.37
116_Q120_I0.6320.37
36_P123_F0.6250.36
9_L18_I0.6200.36
39_I90_L0.6180.35
13_T125_K0.6160.35
13_T18_I0.6140.35
54_L66_L0.6100.35
7_N90_L0.6100.35
107_I115_L0.6080.35
81_P100_E0.6080.35
81_P89_L0.6050.34
24_R70_I0.6030.34
101_L113_N0.6030.34
77_L84_W0.6020.34
71_V79_P0.6010.34
64_L71_V0.5860.33
19_L47_P0.5840.33
64_L108_F0.5830.33
20_Y98_K0.5810.32
26_L50_A0.5740.32
30_A75_L0.5740.32
54_L58_L0.5740.32
68_K120_I0.5640.31
38_S52_W0.5630.31
81_P85_V0.5570.31
20_Y85_V0.5530.30
78_F102_V0.5500.30
33_T37_E0.5480.30
85_V111_I0.5420.29
32_K114_A0.5340.29
38_S89_L0.5330.29
109_P121_D0.5330.29
72_L78_F0.5280.28
81_P88_W0.5270.28
73_L99_L0.5250.28
26_L62_L0.5240.28
10_I29_L0.5240.28
89_L123_F0.5230.28
7_N64_L0.5200.28
51_K77_L0.5160.27
12_T108_F0.5160.27
115_L125_K0.5110.27
21_L62_L0.5080.27
68_K122_S0.5060.27
64_L102_V0.5060.27
9_L101_L0.5020.26
28_R115_L0.5000.26
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1vjeA 2 0.254 2.4 0.95 Contact Map
3al9A 2 0.2857 1.4 0.956 Contact Map
2kv5A 1 0.2143 1 0.96 Contact Map
4il3A 1 0.8016 0.9 0.961 Contact Map
3zo5A 1 0.3333 0.9 0.961 Contact Map
2xphA 1 0.3333 0.8 0.962 Contact Map
4k5yA 1 0.9127 0.8 0.962 Contact Map
1euvA 1 0.3333 0.8 0.963 Contact Map
3r15A 1 0.1429 0.7 0.964 Contact Map
1b3tA 2 0.3413 0.6 0.965 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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