GREMLIN Database
DUF3644 - Protein of unknown function (DUF3644)
PFAM: PF12358 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 77 (77)
Sequences: 3931 (2759)
Seq/√Len: 314.4
META: 0.916

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
49_R53_K3.6641.00
15_Y19_D3.0281.00
63_R67_E2.3621.00
61_P64_K2.3481.00
64_K67_E2.0981.00
45_W50_C2.0821.00
46_E49_R2.0461.00
9_T13_H1.9751.00
2_V6_I1.7591.00
48_R74_N1.7041.00
17_R23_Y1.6271.00
70_I74_N1.5631.00
35_K43_K1.5631.00
6_I10_Y1.5201.00
27_K30_K1.5171.00
60_D64_K1.4691.00
12_L65_N1.4451.00
41_A75_E1.4251.00
59_D62_V1.4141.00
68_F71_E1.3561.00
27_K31_R1.3481.00
16_F50_C1.3381.00
16_F65_N1.3261.00
52_K67_E1.2991.00
32_R35_K1.2791.00
60_D63_R1.2471.00
24_R42_S1.2411.00
23_Y26_R1.2391.00
56_C63_R1.2171.00
25_Y46_E1.2131.00
49_R52_K1.2061.00
36_K42_S1.1981.00
58_L61_P1.1971.00
15_Y54_Y1.1681.00
34_Y42_S1.1461.00
34_Y37_T1.1411.00
45_W49_R1.0631.00
47_L70_I1.0551.00
5_I47_L1.0131.00
38_R43_K0.9881.00
4_M68_F0.9751.00
6_I13_H0.9691.00
8_W12_L0.9571.00
11_L15_Y0.9561.00
10_Y13_H0.9531.00
6_I9_T0.9501.00
74_N77_E0.9421.00
17_R22_D0.9361.00
28_K31_R0.9221.00
48_R67_E0.9111.00
2_V77_E0.9061.00
15_Y68_F0.9031.00
24_R44_T0.8941.00
55_F58_L0.8901.00
48_R52_K0.8811.00
10_Y14_A0.8741.00
51_L73_R0.8651.00
19_D55_F0.8631.00
65_N73_R0.8531.00
14_A73_R0.8331.00
29_S33_K0.8331.00
48_R70_I0.8261.00
70_I73_R0.8191.00
29_S32_R0.8161.00
13_H16_F0.8041.00
15_Y18_R0.7700.99
26_R29_S0.7530.99
13_H65_N0.7510.99
25_Y43_K0.7350.99
66_L73_R0.7320.99
7_A14_A0.7280.99
4_M69_L0.7240.99
37_T40_G0.7140.99
33_K36_K0.7140.99
31_R34_Y0.6930.99
43_K46_E0.6860.99
8_W44_T0.6850.99
9_T21_I0.6810.99
68_F72_L0.6800.99
38_R53_K0.6790.99
8_W65_N0.6710.99
55_F60_D0.6660.98
67_E71_E0.6610.98
58_L62_V0.6540.98
30_K33_K0.6450.98
38_R41_A0.6400.98
5_I9_T0.6370.98
1_V4_M0.6360.98
68_F73_R0.6330.98
23_Y27_K0.6260.98
17_R21_I0.6250.98
28_K32_R0.6180.98
61_P65_N0.6150.97
16_F45_W0.6130.97
57_P60_D0.6090.97
15_Y59_D0.5980.97
16_F73_R0.5950.97
17_R44_T0.5880.97
8_W47_L0.5730.96
39_D71_E0.5710.96
37_T41_A0.5680.96
37_T75_E0.5590.96
5_I8_W0.5540.95
27_K32_R0.5540.95
5_I70_I0.5490.95
1_V76_I0.5450.95
9_T73_R0.5440.95
10_Y17_R0.5390.95
9_T19_D0.5390.95
14_A65_N0.5390.95
15_Y73_R0.5330.94
3_L69_L0.5250.94
54_Y57_P0.5210.94
51_L66_L0.5130.93
13_H74_N0.5010.92
26_R33_K0.5000.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3brjA 2 0.9351 6.4 0.886 Contact Map
3eujB 2 0 5.6 0.889 Contact Map
1ufbA 3 0.7532 4.3 0.895 Contact Map
3c8gA 2 0.9091 4.1 0.896 Contact Map
2mmbA 1 0.5065 2.9 0.903 Contact Map
4xz2A 3 0.8961 2.9 0.903 Contact Map
3opyB 2 0.8312 2.5 0.906 Contact Map
3o8oB 1 0.9481 2.3 0.908 Contact Map
4ykeA 2 0 2.1 0.91 Contact Map
3o8oA 1 0.9481 2 0.911 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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