GREMLIN Database
DUF3549 - Protein of unknown function (DUF3549)
PFAM: PF12069 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 338 (337)
Sequences: 533 (392)
Seq/√Len: 21.3
META: 0.508

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
254_D284_L4.5431.00
156_G159_G3.7691.00
69_I131_F3.6081.00
51_H73_K3.4681.00
326_R335_G3.3461.00
322_R326_R3.3461.00
199_L236_L3.2911.00
322_R335_G3.0380.99
159_G190_A2.9710.99
20_F138_Q2.9610.99
304_F308_V2.9580.99
290_E327_S2.9010.99
172_A205_C2.8730.99
57_L135_A2.8080.99
93_R117_N2.7070.99
326_R334_I2.5840.98
198_P201_A2.5770.98
132_H136_R2.5480.98
143_A177_R2.5430.98
191_L222_R2.5410.98
133_A172_A2.5360.98
314_R318_L2.4220.97
326_R331_A2.4010.97
249_V278_A2.3970.97
253_L278_A2.3930.97
130_A205_C2.3830.97
149_H166_L2.3710.97
154_L171_I2.3570.97
223_L252_L2.3080.96
5_T43_P2.2800.96
134_L209_V2.2690.96
149_H159_G2.2060.95
89_H119_Y2.1910.95
216_A243_S2.1650.95
18_R28_K2.1300.94
273_G276_W2.1060.94
131_F147_Y2.0850.94
211_L216_A2.0780.94
51_H75_P2.0180.93
335_G338_F1.9770.92
162_Q165_F1.9760.92
18_R30_S1.9390.91
171_I201_A1.9350.91
147_Y183_N1.9180.90
217_Q248_L1.8890.90
97_A308_V1.8570.89
6_L321_L1.8480.89
291_R294_Q1.8390.88
75_P86_A1.8270.88
48_L53_W1.8220.88
327_S330_L1.8190.88
56_I166_L1.8030.87
72_L94_L1.7520.86
150_A166_L1.7470.86
306_D310_I1.7410.85
10_L58_F1.7050.84
175_A202_L1.7000.84
96_E99_G1.6820.83
154_L165_F1.6730.83
89_H93_R1.6590.83
283_E287_L1.6440.82
273_G323_S1.6320.81
40_G84_Q1.5970.80
272_A313_L1.5920.80
151_Q174_V1.5830.79
215_L223_L1.5820.79
36_A208_H1.5730.79
178_L183_N1.5720.79
49_Q273_G1.5630.79
143_A148_E1.5530.78
144_S170_G1.5430.78
18_R138_Q1.5330.77
218_A222_R1.5280.77
331_A335_G1.5210.77
17_Y59_W1.5130.76
272_A288_F1.5120.76
93_R116_D1.4970.75
24_R306_D1.4890.75
58_F99_G1.4810.75
156_G276_W1.4690.74
142_P251_Q1.4440.73
223_L236_L1.4390.72
266_E297_Q1.4370.72
167_G198_P1.4120.71
136_R141_Q1.4080.71
326_R338_F1.4050.71
167_G170_G1.3950.70
258_A287_L1.3800.69
195_P288_F1.3690.69
254_D287_L1.3690.69
327_S335_G1.3580.68
148_E151_Q1.3580.68
26_V157_Q1.3420.67
55_A131_F1.3270.66
96_E132_H1.3100.65
9_F15_A1.2990.64
160_W190_A1.2950.64
304_F337_L1.2920.64
303_L314_R1.2850.63
142_P281_D1.2760.63
19_V34_F1.2680.62
97_A304_F1.2670.62
29_I44_Y1.2640.62
38_E87_R1.2620.62
45_P49_Q1.2570.62
202_L219_L1.2460.61
188_A218_A1.2440.61
146_Y167_G1.2390.61
89_H94_L1.2380.61
44_Y59_W1.2290.60
33_Q42_Q1.2170.59
36_A41_Q1.2160.59
141_Q332_Q1.2150.59
11_T35_A1.2060.58
44_Y127_K1.2000.58
97_A119_Y1.1860.57
68_Y132_H1.1840.57
54_L181_D1.1660.56
299_L308_V1.1580.55
179_D267_V1.1450.54
174_V187_L1.1330.54
301_N337_L1.1330.54
6_L313_L1.1320.54
9_F67_P1.1310.54
56_I132_H1.1280.53
25_R268_L1.1240.53
80_G86_A1.1230.53
112_E116_D1.1150.52
147_Y174_V1.1130.52
297_Q301_N1.1020.52
152_A157_Q1.1000.51
26_V133_A1.0990.51
74_L171_I1.0960.51
213_E217_Q1.0920.51
21_D44_Y1.0900.51
149_H165_F1.0900.51
184_E212_P1.0860.50
114_L119_Y1.0700.49
207_E238_R1.0670.49
166_L180_E1.0640.49
290_E326_R1.0620.49
86_A89_H1.0610.49
60_Q67_P1.0580.49
98_L266_E1.0580.49
25_R242_G1.0560.48
90_F99_G1.0540.48
28_K271_I1.0530.48
99_G102_L1.0470.48
92_K96_E1.0470.48
28_K219_L1.0390.47
155_S190_A1.0360.47
149_H162_Q1.0320.47
148_E157_Q1.0310.47
128_M279_L1.0300.47
237_L261_A1.0300.47
22_L135_A1.0280.47
276_W306_D1.0280.47
146_Y170_G1.0280.47
306_D314_R1.0220.46
168_L229_D1.0190.46
43_P161_D1.0150.46
290_E334_I1.0120.46
73_K127_K1.0100.45
147_Y170_G1.0080.45
274_R306_D1.0060.45
104_A108_E1.0010.45
217_Q247_E0.9970.45
107_S179_D0.9970.45
299_L303_L0.9840.44
201_A208_H0.9830.44
55_A67_P0.9820.44
179_D212_P0.9760.43
14_G195_P0.9660.43
68_Y93_R0.9650.42
328_E331_A0.9600.42
40_G86_A0.9550.42
241_S267_V0.9470.41
251_Q281_D0.9420.41
55_A103_T0.9390.41
79_Q192_P0.9320.40
187_L194_L0.9300.40
48_L304_F0.9290.40
150_A174_V0.9280.40
137_A191_L0.9280.40
250_Q271_I0.9270.40
6_L25_R0.9260.40
34_F38_E0.9260.40
69_I130_A0.9220.40
279_L292_L0.9220.40
68_Y94_L0.9190.39
10_L80_G0.9180.39
273_G288_F0.9170.39
200_Y235_A0.9120.39
58_F68_Y0.9120.39
256_L259_S0.9110.39
50_Q270_A0.9110.39
66_Q269_A0.9090.39
154_L167_G0.9080.39
156_G198_P0.9070.39
74_L154_L0.9070.39
75_P87_R0.9050.39
89_H304_F0.9050.39
101_N167_G0.8920.38
23_G55_A0.8890.38
22_L208_H0.8890.38
53_W73_K0.8870.37
290_E294_Q0.8850.37
322_R338_F0.8840.37
143_A157_Q0.8830.37
299_L304_F0.8820.37
74_L214_A0.8780.37
144_S173_D0.8770.37
331_A334_I0.8740.37
55_A139_L0.8620.36
206_L219_L0.8590.36
166_L174_V0.8590.36
26_V138_Q0.8590.36
307_L310_I0.8570.36
69_I113_L0.8550.35
84_Q111_Q0.8540.35
120_A303_L0.8530.35
49_Q54_L0.8470.35
272_A318_L0.8460.35
84_Q88_D0.8460.35
290_E338_F0.8430.35
154_L175_A0.8420.35
58_F90_F0.8410.35
128_M132_H0.8400.35
193_H281_D0.8390.34
93_R132_H0.8340.34
41_Q55_A0.8340.34
242_G307_L0.8330.34
37_I134_L0.8300.34
68_Y89_H0.8280.34
146_Y149_H0.8260.34
285_L317_V0.8180.33
327_S334_I0.8150.33
150_A198_P0.8140.33
54_L132_H0.8120.33
233_L267_V0.8080.33
255_A282_E0.8070.33
151_Q183_N0.8050.32
137_A179_D0.8000.32
294_Q329_A0.7990.32
132_H154_L0.7920.32
252_L255_A0.7910.32
35_A209_V0.7860.31
326_R330_L0.7850.31
17_Y34_F0.7810.31
187_L288_F0.7790.31
92_K119_Y0.7770.31
76_L229_D0.7760.31
146_Y189_K0.7760.31
188_A211_L0.7760.31
188_A214_A0.7730.31
322_R334_I0.7710.30
91_L208_H0.7690.30
226_D239_A0.7670.30
304_F313_L0.7660.30
102_L269_A0.7640.30
133_A162_Q0.7610.30
265_P299_L0.7590.30
5_T96_E0.7580.30
324_P331_A0.7580.30
21_D29_I0.7580.30
256_L267_V0.7540.30
309_A334_I0.7500.29
4_H8_E0.7490.29
141_Q273_G0.7470.29
263_S291_R0.7440.29
9_F58_F0.7440.29
131_F146_Y0.7420.29
74_L117_N0.7420.29
19_V59_W0.7400.29
30_S33_Q0.7370.29
94_L114_L0.7360.29
193_H212_P0.7350.28
5_T132_H0.7330.28
117_N160_W0.7320.28
304_F309_A0.7300.28
164_Q197_E0.7290.28
285_L320_L0.7220.28
46_Y191_L0.7210.28
25_R76_L0.7200.28
334_I338_F0.7190.28
17_Y99_G0.7190.28
50_Q66_Q0.7170.28
239_A327_S0.7160.27
116_D172_A0.7150.27
136_R167_G0.7150.27
16_Q28_K0.7140.27
74_L125_Q0.7130.27
87_R94_L0.7070.27
308_V337_L0.7050.27
26_V276_W0.7050.27
223_L240_L0.7040.27
328_E333_A0.7020.27
101_N128_M0.7010.27
208_H305_A0.7010.27
201_A302_Q0.6990.27
187_L222_R0.6990.27
68_Y136_R0.6960.26
91_L241_S0.6950.26
68_Y308_V0.6910.26
260_P273_G0.6910.26
220_L252_L0.6900.26
154_L198_P0.6900.26
9_F135_A0.6890.26
113_L305_A0.6860.26
88_D94_L0.6850.26
76_L202_L0.6840.26
43_P165_F0.6830.26
135_A212_P0.6830.26
54_L57_L0.6820.26
21_D127_K0.6770.25
124_S332_Q0.6770.25
281_D284_L0.6760.25
90_F276_W0.6740.25
52_A144_S0.6740.25
44_Y52_A0.6720.25
58_F273_G0.6720.25
66_Q76_L0.6710.25
147_Y151_Q0.6700.25
219_L323_S0.6680.25
96_E276_W0.6680.25
6_L72_L0.6660.25
78_E114_L0.6660.25
80_G173_D0.6660.25
268_L288_F0.6660.25
29_I65_Q0.6650.25
176_A208_H0.6650.25
23_G26_V0.6640.25
148_E284_L0.6640.25
187_L212_P0.6630.25
274_R310_I0.6610.25
150_A156_G0.6610.25
167_G177_R0.6580.24
222_R269_A0.6560.24
37_I40_G0.6520.24
16_Q177_R0.6510.24
25_R134_L0.6510.24
191_L248_L0.6500.24
5_T37_I0.6490.24
122_K204_G0.6480.24
10_L34_F0.6470.24
56_I216_A0.6470.24
81_L86_A0.6460.24
196_A210_E0.6460.24
178_L209_V0.6420.24
90_F273_G0.6420.24
66_Q73_K0.6410.24
189_K196_A0.6370.23
85_A302_Q0.6360.23
171_I222_R0.6360.23
122_K198_P0.6350.23
149_H156_G0.6340.23
244_P325_E0.6310.23
166_L201_A0.6300.23
217_Q226_D0.6290.23
191_L199_L0.6290.23
115_K122_K0.6230.23
183_N206_L0.6200.23
69_I253_L0.6200.23
174_V328_E0.6190.23
327_S331_A0.6180.23
280_E312_G0.6180.23
324_P335_G0.6150.22
271_I298_A0.6150.22
32_E36_A0.6150.22
276_W310_I0.6140.22
8_E22_L0.6140.22
111_Q303_L0.6130.22
46_Y241_S0.6130.22
78_E314_R0.6130.22
266_E301_N0.6120.22
87_R322_R0.6110.22
79_Q83_K0.6100.22
21_D53_W0.6090.22
20_F55_A0.6080.22
65_Q141_Q0.6060.22
49_Q276_W0.6050.22
191_L225_A0.6050.22
53_W57_L0.6050.22
102_L106_L0.6030.22
150_A171_I0.6030.22
141_Q221_E0.6020.22
20_F57_L0.6000.22
194_L313_L0.5990.22
171_I320_L0.5990.22
10_L49_Q0.5980.22
192_P218_A0.5980.22
237_L270_A0.5970.22
81_L120_A0.5970.22
250_Q281_D0.5950.21
156_G160_W0.5930.21
58_F75_P0.5930.21
98_L274_R0.5920.21
214_A220_L0.5880.21
10_L69_I0.5870.21
149_H160_W0.5860.21
127_K208_H0.5860.21
16_Q334_I0.5850.21
153_Y198_P0.5840.21
290_E320_L0.5820.21
117_N308_V0.5770.21
249_V275_C0.5770.21
15_A83_K0.5750.21
220_L258_A0.5750.21
83_K303_L0.5750.21
73_K204_G0.5750.21
48_L54_L0.5730.21
214_A258_A0.5720.20
187_L306_D0.5710.20
164_Q318_L0.5700.20
208_H277_E0.5690.20
13_S289_L0.5690.20
176_A209_V0.5670.20
280_E296_E0.5670.20
286_E320_L0.5660.20
48_L119_Y0.5660.20
137_A194_L0.5640.20
315_P319_Q0.5620.20
236_L259_S0.5620.20
34_F288_F0.5610.20
119_Y122_K0.5600.20
321_L334_I0.5580.20
58_F86_A0.5580.20
50_Q288_F0.5570.20
36_A62_K0.5570.20
74_L273_G0.5560.20
48_L269_A0.5550.20
137_A210_E0.5540.20
299_L302_Q0.5530.20
272_A314_R0.5510.20
24_R38_E0.5510.20
97_A313_L0.5510.20
54_L174_V0.5500.20
175_A317_V0.5490.19
97_A337_L0.5480.19
73_K102_L0.5480.19
209_V262_L0.5450.19
97_A309_A0.5450.19
191_L218_A0.5400.19
3_I99_G0.5400.19
257_L268_L0.5400.19
38_E288_F0.5400.19
6_L20_F0.5400.19
29_I223_L0.5400.19
20_F226_D0.5390.19
211_L220_L0.5390.19
63_Q189_K0.5370.19
10_L285_L0.5360.19
305_A318_L0.5350.19
37_I138_Q0.5350.19
161_D165_F0.5350.19
26_V205_C0.5320.19
140_G176_A0.5320.19
3_I27_Q0.5310.19
88_D269_A0.5310.19
149_H158_L0.5310.19
40_G74_L0.5300.19
317_V321_L0.5300.19
68_Y97_A0.5300.19
265_P296_E0.5290.19
20_F223_L0.5290.19
20_F60_Q0.5280.19
54_L161_D0.5280.19
205_C222_R0.5260.19
88_D309_A0.5260.19
157_Q190_A0.5260.19
42_Q50_Q0.5250.18
254_D258_A0.5250.18
68_Y87_R0.5240.18
25_R289_L0.5210.18
14_G117_N0.5210.18
27_Q106_L0.5200.18
112_E273_G0.5190.18
79_Q272_A0.5190.18
113_L116_D0.5180.18
10_L35_A0.5170.18
143_A332_Q0.5160.18
199_L222_R0.5160.18
175_A182_N0.5160.18
69_I293_A0.5140.18
221_E225_A0.5140.18
116_D133_A0.5110.18
177_R206_L0.5110.18
59_W330_L0.5100.18
113_L302_Q0.5100.18
219_L224_A0.5090.18
241_S253_L0.5080.18
289_L320_L0.5080.18
23_G48_L0.5070.18
37_I240_L0.5040.18
203_C216_A0.5040.18
94_L240_L0.5030.18
148_E214_A0.5030.18
28_K140_G0.5030.18
131_F216_A0.5030.18
171_I306_D0.5030.18
30_S224_A0.5020.18
178_L185_A0.5020.18
189_K220_L0.5020.18
91_L132_H0.5000.18
8_E35_A0.5000.18
22_L175_A0.5000.18
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4ksaA 3 0.4379 45.9 0.972 Contact Map
4ksfA 3 0.3462 38.4 0.973 Contact Map
3ltjA 2 0.5533 31.9 0.974 Contact Map
1oyzA 1 0.6746 25 0.976 Contact Map
4d4zA 1 0.5828 21.9 0.976 Contact Map
4o8wA 3 0.2692 17.9 0.977 Contact Map
2bptA 1 0.429 16.8 0.977 Contact Map
2yd0A 1 0.9083 15.1 0.978 Contact Map
4ks9A 2 0.3107 14.3 0.978 Contact Map
3ltmA 1 0.5355 14.3 0.978 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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