GREMLIN Database
DUF3526 - Domain of unknown function (DUF3526)
PFAM: PF12040 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 144 (138)
Sequences: 2903 (2210)
Seq/√Len: 188.1
META: 0.821

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
37_S40_D4.2931.00
93_A98_D3.2901.00
52_E55_E3.0031.00
102_H106_L2.9701.00
51_Q55_E2.6321.00
114_E118_R2.5261.00
88_Q92_M2.4991.00
20_A23_K2.4741.00
25_K29_R2.4691.00
43_W48_A2.4491.00
36_D40_D2.3801.00
114_E117_R2.3611.00
98_D101_A2.2331.00
58_A62_D2.2231.00
24_D28_A2.1561.00
73_R77_R2.1231.00
20_A24_D2.0761.00
22_L48_A1.9891.00
29_R55_E1.8031.00
115_D119_F1.7861.00
62_D66_E1.7721.00
104_R108_Q1.7091.00
21_A25_K1.6731.00
69_E100_A1.6601.00
26_F48_A1.6251.00
130_V134_D1.5771.00
132_A136_A1.5341.00
116_L120_F1.5171.00
117_R120_F1.4841.00
13_N17_P1.4731.00
63_A67_K1.4581.00
13_N16_D1.3801.00
117_R121_Y1.2991.00
72_N76_R1.2751.00
121_Y124_I1.2581.00
56_E59_E1.2461.00
76_R89_R1.2141.00
122_P125_F1.2121.00
134_D137_S1.2001.00
133_E137_S1.1831.00
104_R143_Y1.1641.00
107_D111_A1.1611.00
59_E62_D1.1461.00
114_E121_Y1.1461.00
119_F123_K1.1361.00
87_L90_L1.1351.00
123_K128_Y1.1231.00
70_R73_R1.1191.00
35_V42_P1.0981.00
38_V45_W1.0961.00
121_Y125_F1.0871.00
108_Q141_F1.0861.00
41_L47_G1.0591.00
131_D134_D1.0561.00
118_R121_Y1.0541.00
73_R76_R1.0501.00
72_N99_L1.0441.00
4_F7_Q1.0331.00
47_G50_Q1.0301.00
30_Y43_W1.0281.00
15_W18_P1.0261.00
4_F9_E1.0231.00
59_E63_A1.0091.00
23_K27_L1.0071.00
78_L85_L1.0050.99
119_F122_P1.0040.99
36_D39_E1.0000.99
5_L9_E1.0000.99
26_F29_R0.9960.99
123_K134_D0.9910.99
64_Y67_K0.9860.99
19_F23_K0.9790.99
45_W53_G0.9790.99
22_L26_F0.9640.99
54_E113_R0.9590.99
35_V40_D0.9470.99
72_N90_L0.9400.99
79_G85_L0.9390.99
27_L34_G0.9390.99
103_L107_D0.9360.99
79_G89_R0.9280.99
6_A10_A0.9240.99
7_Q10_A0.9220.99
39_E42_P0.9200.99
11_G15_W0.9160.99
108_Q111_A0.9120.99
77_R80_L0.9060.99
74_L77_R0.9020.99
12_H16_D0.9010.99
119_F128_Y0.8970.99
99_L103_L0.8920.99
122_P126_R0.8900.99
57_S61_Y0.8900.99
50_Q57_S0.8870.99
120_F124_I0.8860.99
69_E73_R0.8750.99
16_D19_F0.8720.99
30_Y55_E0.8720.99
50_Q54_E0.8680.99
116_L121_Y0.8630.98
68_L72_N0.8590.98
77_R81_L0.8580.98
132_A138_L0.8580.98
52_E56_E0.8530.98
11_G18_P0.8520.98
86_A92_M0.8430.98
115_D118_R0.8400.98
79_G86_A0.8390.98
91_S113_R0.8210.98
105_F109_A0.8200.98
41_L45_W0.8160.98
120_F123_K0.8100.98
4_F8_R0.8080.98
103_L106_L0.7920.97
14_P18_P0.7860.97
116_L119_F0.7850.97
13_N21_A0.7830.97
133_E136_A0.7820.97
53_G57_S0.7780.97
17_P21_A0.7720.97
47_G53_G0.7600.97
43_W46_Y0.7560.96
114_E127_D0.7480.96
117_R122_P0.7480.96
21_A111_A0.7470.96
69_E103_L0.7410.96
46_Y126_R0.7400.96
96_G101_A0.7380.96
118_R123_K0.7310.96
108_Q143_Y0.7300.96
120_F125_F0.7180.95
119_F125_F0.7140.95
75_A85_L0.7120.95
124_I127_D0.7100.95
11_G53_G0.7080.95
5_L10_A0.7080.95
65_A68_L0.7000.95
120_F128_Y0.6970.94
43_W47_G0.6940.94
46_Y50_Q0.6850.94
123_K126_R0.6800.94
64_Y68_L0.6750.93
11_G22_L0.6690.93
131_D137_S0.6690.93
8_R15_W0.6690.93
130_V138_L0.6610.93
75_A92_M0.6600.93
15_W26_F0.6590.93
54_E57_S0.6550.92
41_L44_N0.6530.92
130_V135_W0.6480.92
102_H138_L0.6450.92
132_A137_S0.6440.92
125_F128_Y0.6410.92
65_A106_L0.6380.91
59_E69_E0.6210.90
67_K70_R0.6210.90
62_D65_A0.6200.90
65_A107_D0.6140.90
109_A113_R0.6140.90
66_E70_R0.6110.90
43_W54_E0.6040.89
131_D136_A0.5950.88
26_F53_G0.5930.88
5_L8_R0.5910.88
117_R128_Y0.5900.88
49_V106_L0.5810.87
62_D100_A0.5750.87
121_Y127_D0.5740.87
15_W19_F0.5670.86
128_Y134_D0.5640.86
82_S85_L0.5630.85
113_R121_Y0.5540.85
46_Y125_F0.5530.84
50_Q117_R0.5530.84
23_K37_S0.5520.84
128_Y132_A0.5510.84
90_L111_A0.5500.84
18_P21_A0.5480.84
70_R77_R0.5420.83
44_N54_E0.5400.83
79_G101_A0.5340.82
45_W49_V0.5320.82
23_K38_V0.5310.82
49_V52_E0.5250.81
114_E119_F0.5220.81
82_S86_A0.5220.81
108_Q112_Y0.5200.81
116_L124_I0.5180.80
21_A24_D0.5180.80
49_V54_E0.5180.80
131_D135_W0.5160.80
11_G14_P0.5150.80
56_E68_L0.5140.80
48_A52_E0.5130.80
18_P45_W0.5130.80
23_K110_E0.5130.80
15_W20_A0.5100.79
15_W45_W0.5080.79
56_E60_V0.5070.79
110_E113_R0.5060.79
112_Y139_P0.5050.79
23_K85_L0.5030.78
10_A85_L0.5020.78
27_L38_V0.5010.78
95_A133_E0.5010.78
84_A88_Q0.5000.78
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3ieeA 1 0.7917 5.4 0.923 Contact Map
1y66A 3 0.3472 5.3 0.923 Contact Map
2etdA 1 0.7292 4.1 0.927 Contact Map
3rh3A 1 0.8611 3.7 0.929 Contact Map
1x3kA 1 0.3819 3.5 0.93 Contact Map
4f4fA 1 0.3681 2.9 0.933 Contact Map
3w4uA 2 0.3611 2.6 0.934 Contact Map
1wddA 4 0.3681 2.5 0.935 Contact Map
1it2A 1 0.4236 2.5 0.935 Contact Map
3bomA 2 0.3611 2.1 0.937 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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