GREMLIN Database
DUF3515 - Protein of unknown function (DUF3515)
PFAM: PF12028 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 158 (142)
Sequences: 1788 (1397)
Seq/√Len: 117.2
META: 0.521

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
83_R131_E3.7941.00
88_R129_Y3.0081.00
72_W82_L2.9661.00
45_L82_L2.8971.00
100_E105_D2.7431.00
132_V146_L2.5951.00
102_N148_D2.3521.00
36_D39_A2.2911.00
14_A17_L2.2551.00
49_L70_A2.2181.00
138_S141_T2.0951.00
32_V35_P2.0851.00
29_V32_V2.0231.00
58_R70_A1.7121.00
98_L123_T1.7041.00
109_V120_T1.6851.00
118_G133_T1.6681.00
53_L149_A1.6541.00
14_A18_L1.6471.00
61_V71_A1.6371.00
59_R73_G1.6241.00
111_D120_T1.6101.00
75_P135_P1.6051.00
10_A13_A1.6001.00
61_V69_T1.5851.00
45_L130_V1.5551.00
91_E109_V1.5481.00
34_A37_A1.5201.00
109_V122_T1.5041.00
10_A14_A1.4541.00
145_D148_D1.4341.00
86_V122_T1.4201.00
113_D136_A1.4191.00
120_T133_T1.4141.00
108_V119_T1.4021.00
123_T150_I1.3911.00
86_V131_E1.3891.00
53_L145_D1.3831.00
13_A16_A1.3281.00
122_T131_E1.3251.00
61_V65_A1.2961.00
123_T129_Y1.2921.00
66_S69_T1.2730.99
149_A153_T1.2660.99
104_V123_T1.2380.99
27_V30_V1.2300.99
91_E96_S1.2100.99
83_R86_V1.2080.99
111_D118_G1.2060.99
92_F95_T1.2000.99
10_A17_L1.1890.99
92_F124_V1.1870.99
8_V11_V1.1840.99
35_P38_D1.1780.99
56_L80_I1.1730.99
121_W134_V1.1650.99
12_L16_A1.1600.99
8_V12_L1.1580.99
104_V147_S1.1560.99
9_V17_L1.1540.99
41_A44_A1.1470.99
88_R92_F1.1360.99
48_A153_T1.1200.99
120_T131_E1.1140.99
101_V143_L1.1020.98
46_L70_A1.0980.98
128_V155_P1.0900.98
11_V17_L1.0870.98
145_D149_A1.0630.98
94_P107_F1.0420.98
7_L15_A1.0350.98
92_F107_F1.0240.98
46_L67_P1.0240.98
91_E107_F1.0210.98
100_E103_G1.0080.97
137_G140_P1.0070.97
108_V121_W0.9910.97
24_T27_V0.9750.97
52_T55_G0.9650.96
21_A24_T0.9640.96
39_A68_G0.9630.96
123_T126_R0.9610.96
48_A152_A0.9570.96
106_W147_S0.9540.96
59_R76_A0.9440.96
92_F96_S0.9290.96
28_A32_V0.9230.95
17_L20_A0.9200.95
98_L125_D0.9170.95
25_G29_V0.9120.95
12_L17_L0.9060.95
13_A17_L0.9040.95
98_L105_D0.9030.95
7_L10_A0.9020.95
139_G142_P0.9000.95
27_V32_V0.8950.95
137_G141_T0.8890.94
56_L74_D0.8770.94
83_R133_T0.8710.94
110_E119_T0.8620.94
51_D58_R0.8610.93
33_P87_P0.8570.93
40_A68_G0.8540.93
151_A156_A0.8490.93
89_P107_F0.8370.92
7_L18_L0.8280.92
16_A19_S0.8170.92
64_P67_P0.8150.91
101_V147_S0.8130.91
59_R71_A0.8110.91
10_A15_A0.8070.91
29_V61_V0.7980.91
17_L21_A0.7960.90
7_L13_A0.7910.90
61_V64_P0.7820.90
9_V13_A0.7810.90
118_G134_V0.7800.90
139_G143_L0.7770.89
73_G122_T0.7710.89
130_V135_P0.7700.89
64_P68_G0.7640.89
53_L146_L0.7600.88
93_T96_S0.7580.88
34_A40_A0.7560.88
109_V113_D0.7540.88
148_D152_A0.7530.88
73_G134_V0.7530.88
22_A25_G0.7510.88
18_L21_A0.7510.88
121_W139_G0.7470.87
64_P129_Y0.7450.87
45_L155_P0.7410.87
29_V69_T0.7400.87
44_A154_L0.7360.87
19_S22_A0.7360.87
40_A43_A0.7310.86
99_Q121_W0.7260.86
143_L147_S0.7240.86
13_A19_S0.7220.86
99_Q150_I0.7180.85
95_T98_L0.7180.85
8_V16_A0.7160.85
131_E150_I0.7110.85
22_A28_A0.7030.84
141_T144_Q0.7010.84
24_T28_A0.7000.84
110_E123_T0.6900.83
121_W140_P0.6890.83
49_L82_L0.6880.83
109_V112_D0.6880.83
23_S26_P0.6870.83
140_P144_Q0.6860.83
89_P129_Y0.6830.82
83_R107_F0.6830.82
108_V143_L0.6770.82
98_L108_V0.6740.82
147_S151_A0.6740.82
7_L11_V0.6680.81
23_S28_A0.6630.80
26_P32_V0.6620.80
97_P108_V0.6610.80
128_V154_L0.6570.80
43_A70_A0.6560.80
15_A18_L0.6480.79
142_P147_S0.6460.79
8_V14_A0.6340.77
113_D118_G0.6310.77
106_W145_D0.6290.77
142_P146_L0.6220.76
64_P69_T0.6200.76
153_T156_A0.6180.76
144_Q150_I0.6150.75
98_L124_V0.6130.75
52_T72_W0.6080.75
33_P41_A0.6050.74
43_A68_G0.6030.74
44_A48_A0.6020.74
10_A16_A0.5980.73
105_D125_D0.5830.71
32_V37_A0.5800.71
13_A18_L0.5800.71
11_V18_L0.5760.71
80_I101_V0.5750.70
86_V89_P0.5720.70
87_P131_E0.5680.69
105_D124_V0.5660.69
16_A20_A0.5630.69
124_V150_I0.5610.69
36_D40_A0.5550.68
30_V36_D0.5540.68
63_E70_A0.5510.67
53_L138_S0.5500.67
42_C84_C0.5500.67
106_W132_V0.5480.67
91_E94_P0.5440.66
61_V66_S0.5440.66
25_G60_P0.5340.65
23_S30_V0.5330.65
11_V14_A0.5290.64
30_V33_P0.5280.64
43_A67_P0.5270.64
102_N144_Q0.5270.64
25_G30_V0.5260.64
7_L12_L0.5250.63
26_P60_P0.5220.63
30_V81_V0.5190.63
28_A37_A0.5150.62
45_L72_W0.5120.62
72_W88_R0.5110.61
72_W83_R0.5090.61
52_T93_T0.5060.61
125_D129_Y0.5030.60
46_L49_L0.5000.60
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4fazA 4 0.1962 6.9 0.941 Contact Map
3ry0A 4 0.1962 6.7 0.941 Contact Map
4x1cC 5 0.1962 6.7 0.941 Contact Map
2opaA 4 0.1962 6.5 0.941 Contact Map
2x4kA 4 0.2152 5.9 0.942 Contact Map
3m21A 4 0.1962 5.9 0.942 Contact Map
4fdxA 4 0.1962 5.6 0.943 Contact Map
3ej9B 3 0.1962 5.3 0.943 Contact Map
3m20A 5 0.1899 5 0.944 Contact Map
3ej9A 3 0.1962 4 0.947 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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