GREMLIN Database
DUF3504 - Domain of unknown function (DUF3504)
PFAM: PF12012 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 161 (153)
Sequences: 832 (728)
Seq/√Len: 58.9
META: 0.571

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
75_K79_G3.9461.00
17_G23_G3.8981.00
65_E109_K3.7261.00
44_H127_Y3.4461.00
110_L114_K3.0161.00
54_L109_K2.8441.00
21_P141_W2.4751.00
116_P128_L2.4331.00
76_T79_G2.3651.00
45_R146_P2.3581.00
25_L147_L2.3371.00
52_F108_Y2.3071.00
54_L108_Y2.2821.00
27_T107_L2.2371.00
41_R153_G2.2361.00
7_E11_W2.1411.00
115_R128_L2.0981.00
47_L127_Y2.0201.00
27_T31_F1.9681.00
77_R80_G1.9651.00
20_S23_G1.9541.00
80_G83_K1.8391.00
49_F112_L1.8361.00
74_S79_G1.8201.00
29_W33_T1.7570.99
17_G27_T1.7010.99
25_L142_Y1.6650.99
49_F115_R1.6390.99
31_F107_L1.5780.99
8_E102_R1.5640.98
38_L43_E1.5000.98
78_T82_K1.4750.98
75_K78_T1.4700.98
131_K143_S1.4660.98
79_G83_K1.4440.97
23_G27_T1.4410.97
93_M96_N1.4310.97
9_K13_S1.4090.97
116_P127_Y1.4020.97
20_S142_Y1.3640.96
42_E46_Q1.3490.96
74_S77_R1.3350.96
76_T80_G1.3280.96
107_L142_Y1.3180.96
57_D60_G1.3110.95
67_L108_Y1.2210.93
32_N104_P1.1990.93
7_E34_K1.1900.92
75_K81_L1.1880.92
25_L115_R1.1870.92
16_L27_T1.1850.92
47_L51_D1.1790.92
55_K68_E1.1650.92
31_F104_P1.1630.91
129_R145_Q1.1340.90
78_T81_L1.1200.90
77_R82_K1.1170.90
41_R45_R1.1130.90
69_Y72_P1.1110.89
65_E95_E1.0820.88
18_T107_L1.0690.88
80_G86_E1.0660.87
48_K51_D1.0570.87
19_D110_L1.0360.86
6_E9_K1.0270.85
32_N126_F1.0230.85
93_M104_P1.0140.85
25_L32_N1.0000.84
49_F128_L0.9990.84
102_R142_Y0.9990.84
74_S78_T0.9950.84
129_R143_S0.9920.83
76_T83_K0.9760.82
41_R149_K0.9630.82
8_E12_E0.9500.81
18_T27_T0.9480.80
32_N36_F0.9470.80
131_K138_S0.9430.80
74_S80_G0.9170.78
128_L141_W0.9140.78
29_W159_I0.9140.78
89_K94_Y0.9120.78
79_G82_K0.9060.77
51_D127_Y0.9050.77
150_N153_G0.8990.77
23_G107_L0.8930.76
66_Y69_Y0.8900.76
38_L127_Y0.8880.76
43_E116_P0.8870.76
78_T83_K0.8870.76
106_K110_L0.8850.76
104_P141_W0.8850.76
65_E69_Y0.8790.75
28_V115_R0.8720.75
83_K86_E0.8680.74
11_W107_L0.8600.74
77_R81_L0.8530.73
25_L111_Y0.8530.73
114_K141_W0.8510.73
49_F126_F0.8430.72
154_K157_K0.8420.72
29_W157_K0.8410.72
3_S6_E0.8400.72
33_T44_H0.8400.72
148_G152_L0.8340.71
50_S115_R0.8260.71
26_N155_M0.8180.70
52_F126_F0.8070.69
110_L113_S0.8040.68
26_N45_R0.8010.68
25_L44_H0.7990.68
54_L67_L0.7880.67
151_T154_K0.7860.67
97_P100_P0.7860.67
36_F104_P0.7830.66
73_E78_T0.7770.66
75_K83_K0.7740.66
7_E107_L0.7720.65
136_P139_P0.7720.65
100_P106_K0.7700.65
23_G110_L0.7700.65
28_V126_F0.7620.64
25_L155_M0.7610.64
77_R86_E0.7600.64
27_T44_H0.7550.64
43_E51_D0.7480.63
32_N127_Y0.7480.63
22_Q151_T0.7460.63
111_Y126_F0.7430.62
114_K140_V0.7380.62
129_R151_T0.7380.62
31_F35_Y0.7330.61
54_L152_L0.7330.61
44_H49_F0.7310.61
29_W44_H0.7300.61
29_W155_M0.7270.61
14_G19_D0.7270.61
14_G17_G0.7260.61
11_W31_F0.7240.60
95_E106_K0.7240.60
33_T159_I0.7220.60
57_D94_Y0.7200.60
65_E72_P0.7190.60
33_T38_L0.7160.59
11_W27_T0.7160.59
39_R146_P0.7150.59
75_K80_G0.7140.59
81_L86_E0.7130.59
71_E75_K0.7130.59
30_F155_M0.7060.58
26_N158_R0.7040.58
45_R152_L0.7030.58
17_G20_S0.6990.58
35_Y104_P0.6970.57
71_E74_S0.6850.56
116_P143_S0.6810.56
133_S136_P0.6780.55
118_S144_N0.6770.55
58_E142_Y0.6770.55
77_R83_K0.6670.54
95_E103_C0.6580.53
43_E148_G0.6560.53
29_W51_D0.6550.53
67_L113_S0.6540.53
50_S119_C0.6440.52
8_E15_Q0.6400.51
92_R96_N0.6370.51
151_T158_R0.6360.51
149_K153_G0.6340.50
10_L159_I0.6320.50
38_L116_P0.6320.50
57_D61_R0.6290.50
82_K86_E0.6260.50
29_W156_M0.6240.49
24_L48_K0.6210.49
79_G84_R0.6190.49
57_D66_Y0.6190.49
5_E22_Q0.6150.48
78_T84_R0.6060.47
33_T37_G0.6020.47
3_S9_K0.6000.47
121_A132_R0.5980.46
85_K88_P0.5960.46
8_E11_W0.5950.46
49_F120_K0.5910.46
33_T111_Y0.5860.45
30_F33_T0.5850.45
40_G45_R0.5810.45
80_G84_R0.5800.45
45_R155_M0.5800.45
135_S138_S0.5710.44
81_L84_R0.5690.43
20_S154_K0.5670.43
25_L127_Y0.5590.42
6_E23_G0.5590.42
54_L112_L0.5580.42
54_L65_E0.5570.42
148_G151_T0.5550.42
76_T81_L0.5520.41
35_Y108_Y0.5490.41
148_G153_G0.5470.41
44_H80_G0.5460.41
103_C147_L0.5420.40
147_L151_T0.5410.40
98_T106_K0.5410.40
94_Y97_P0.5410.40
30_F111_Y0.5400.40
90_V137_D0.5380.40
21_P71_E0.5370.40
3_S10_L0.5300.39
51_D71_E0.5270.39
69_Y73_E0.5230.38
37_G129_R0.5200.38
31_F44_H0.5190.38
4_R12_E0.5180.38
130_P143_S0.5170.38
51_D120_K0.5150.38
11_W44_H0.5150.38
21_P128_L0.5140.37
38_L44_H0.5130.37
96_N99_N0.5130.37
74_S83_K0.5120.37
23_G138_S0.5110.37
156_M159_I0.5080.37
54_L69_Y0.5080.37
41_R152_L0.5050.36
93_M97_P0.5050.36
147_L155_M0.5040.36
95_E98_T0.5030.36
21_P117_P0.5000.36
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3nkhA 2 0.8199 85.5 0.891 Contact Map
2a3vA 3 0.7764 80.7 0.897 Contact Map
4a8eA 2 0.7081 80.6 0.897 Contact Map
1xo0A 3 0.8199 78.8 0.899 Contact Map
1a0pA 1 0.7391 78.7 0.899 Contact Map
1ae9A 4 0.7205 72 0.904 Contact Map
1z19A 3 0.7205 70.4 0.905 Contact Map
1z1bA 3 0.7205 61.2 0.911 Contact Map
1aihA 2 0.7267 14.6 0.937 Contact Map
2jubA 1 0.1801 3.6 0.952 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0073 seconds.