GREMLIN Database
DUF3500 - Protein of unknown function (DUF3500)
PFAM: PF12006 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 295 (290)
Sequences: 7360 (5164)
Seq/√Len: 303.2
META: 0.908

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
201_I206_L4.4551.00
175_K205_E3.6071.00
251_G256_S3.4961.00
203_A214_L3.0301.00
165_L252_T2.7491.00
17_S62_A2.7151.00
25_K54_E2.7101.00
215_L238_K2.6461.00
253_E256_S2.4441.00
165_L177_I2.3581.00
211_R242_D2.3461.00
169_D172_Q2.3291.00
77_E81_D2.3271.00
172_Q210_Q2.3081.00
28_F97_S2.2891.00
19_D22_Q2.2141.00
160_L220_E2.1671.00
64_L80_R2.1661.00
152_R159_D2.1491.00
79_A117_L2.1161.00
15_L27_T2.0961.00
169_D207_T2.0701.00
14_F26_A2.0291.00
14_F63_A2.0181.00
64_L76_Y1.9671.00
227_D231_A1.9581.00
11_A66_L1.9391.00
240_A266_T1.9241.00
219_E223_G1.9191.00
235_A239_A1.9071.00
96_F112_F1.8941.00
215_L241_L1.8661.00
61_E65_A1.8641.00
171_E174_K1.8451.00
167_S213_L1.8231.00
202_P245_H1.7991.00
235_A238_K1.7951.00
29_P32_D1.7811.00
170_A173_R1.7671.00
50_L55_M1.7221.00
23_R27_T1.7171.00
143_V146_G1.6921.00
207_T210_Q1.6851.00
222_V234_M1.6571.00
178_L247_A1.6511.00
202_P243_K1.6351.00
162_R166_N1.6321.00
219_E234_M1.6161.00
173_R177_I1.6091.00
68_R76_Y1.6081.00
163_A213_L1.5951.00
7_M70_G1.5881.00
203_A211_R1.5631.00
12_N101_T1.5621.00
14_F18_L1.5541.00
76_Y80_R1.5461.00
78_K220_E1.5371.00
34_E37_R1.5181.00
172_Q201_I1.5151.00
185_D188_T1.5151.00
170_A174_K1.5101.00
208_A211_R1.4951.00
191_R194_A1.4841.00
113_E118_S1.4811.00
142_E152_R1.4771.00
8_A12_N1.4571.00
12_N16_A1.4511.00
70_G110_W1.4451.00
287_R292_D1.4291.00
56_T59_Q1.4231.00
87_E90_G1.4121.00
30_F122_T1.4041.00
212_A216_D1.3921.00
202_P205_E1.3771.00
5_A9_A1.3711.00
177_I252_T1.3711.00
18_L26_A1.3461.00
156_A220_E1.3451.00
87_E91_E1.3301.00
7_M123_V1.3281.00
38_W42_P1.3241.00
80_R83_M1.3231.00
262_I269_I1.3221.00
152_R158_E1.3201.00
85_L153_V1.3171.00
148_G151_L1.3171.00
73_A77_E1.3161.00
231_A235_A1.3121.00
213_L216_D1.3111.00
72_S75_G1.3111.00
160_L163_A1.3071.00
52_L63_A1.2981.00
21_E24_A1.2961.00
107_P124_V1.2911.00
70_G121_F1.2871.00
65_A68_R1.2831.00
22_Q50_L1.2791.00
57_P61_E1.2741.00
51_P54_E1.2631.00
51_P95_Y1.2611.00
221_Y284_T1.2601.00
233_R236_E1.2591.00
221_Y260_Y1.2451.00
146_G150_G1.2321.00
160_L213_L1.2281.00
169_D210_Q1.2171.00
167_S210_Q1.2121.00
22_Q59_Q1.2101.00
9_A13_A1.2041.00
11_A98_I1.1981.00
152_R155_G1.1891.00
164_L214_L1.1781.00
145_E149_K1.1751.00
198_P245_H1.1731.00
43_F46_E1.1731.00
147_P150_G1.1711.00
28_F34_E1.1661.00
215_L234_M1.1631.00
13_A65_A1.1521.00
40_N189_A1.1471.00
60_R92_E1.1441.00
15_L26_A1.1441.00
38_W41_I1.1411.00
257_P279_A1.1391.00
218_I269_I1.1371.00
155_G159_D1.1271.00
168_L206_L1.1241.00
84_R148_G1.1221.00
159_D163_A1.1191.00
203_A206_L1.1131.00
214_L262_I1.1131.00
166_N253_E1.1121.00
20_P23_R1.1111.00
164_L213_L1.0951.00
164_L206_L1.0941.00
168_L176_A1.0911.00
78_K157_E1.0871.00
157_E220_E1.0861.00
55_M63_A1.0801.00
162_R254_P1.0791.00
175_K201_I1.0781.00
136_F224_R1.0701.00
198_P263_Q1.0641.00
17_S59_Q1.0571.00
81_D84_R1.0521.00
208_A212_A1.0451.00
203_A241_L1.0341.00
39_H190_N1.0341.00
173_R252_T1.0241.00
117_L136_F1.0201.00
53_K92_E1.0121.00
63_A96_F1.0091.00
160_L217_L1.0091.00
30_F35_R1.0051.00
185_D189_A1.0041.00
74_R78_K0.9981.00
163_A216_D0.9901.00
39_H45_V0.9871.00
165_L176_A0.9851.00
67_L112_F0.9761.00
250_G253_E0.9741.00
82_I136_F0.9731.00
243_K265_P0.9721.00
188_T191_R0.9711.00
13_A62_A0.9691.00
8_A104_A0.9651.00
241_L244_T0.9641.00
158_E162_R0.9641.00
166_N254_P0.9611.00
81_D153_V0.9601.00
178_L249_A0.9591.00
201_I205_E0.9591.00
79_A136_F0.9581.00
160_L216_D0.9581.00
172_Q207_T0.9571.00
78_K224_R0.9531.00
81_D151_L0.9491.00
57_P60_R0.9471.00
240_A265_P0.9471.00
58_A61_E0.9421.00
267_F289_P0.9381.00
250_G254_P0.9341.00
181_T184_D0.9311.00
142_E254_P0.9281.00
258_H271_Y0.9271.00
257_P274_T0.9231.00
138_A153_V0.9201.00
162_R271_Y0.9141.00
188_T194_A0.9101.00
61_E68_R0.9061.00
52_L96_F0.9001.00
83_M112_F0.9001.00
209_A212_A0.8921.00
267_F286_W0.8831.00
121_F131_M0.8781.00
13_A16_A0.8771.00
35_R122_T0.8731.00
218_I241_L0.8711.00
14_F55_M0.8681.00
244_T262_I0.8681.00
165_L173_R0.8671.00
215_L219_E0.8661.00
39_H42_P0.8651.00
51_P93_L0.8631.00
55_M59_Q0.8621.00
37_R45_V0.8611.00
204_S242_D0.8571.00
263_Q289_P0.8551.00
143_V148_G0.8501.00
112_F117_L0.8501.00
20_P24_A0.8491.00
67_L79_A0.8481.00
225_L233_R0.8481.00
224_R233_R0.8441.00
158_E271_Y0.8411.00
187_L194_A0.8391.00
244_T267_F0.8381.00
216_D219_E0.8381.00
156_A160_L0.8381.00
109_G120_N0.8311.00
128_Q131_M0.8201.00
259_Y270_E0.8201.00
189_A192_D0.8201.00
218_I244_T0.8181.00
229_L232_A0.8101.00
53_K93_L0.8101.00
166_N173_R0.8081.00
180_D183_P0.8081.00
64_L68_R0.8071.00
80_R84_R0.8061.00
74_R223_G0.8051.00
39_H44_Y0.8021.00
225_L229_L0.8001.00
105_T125_D0.7941.00
28_F48_K0.7901.00
134_S224_R0.7891.00
211_R215_L0.7891.00
161_A217_L0.7891.00
7_M110_W0.7880.99
220_E223_G0.7870.99
143_V151_L0.7840.99
36_R192_D0.7820.99
259_Y272_D0.7810.99
74_R77_E0.7790.99
275_Q278_N0.7770.99
41_I44_Y0.7750.99
125_D128_Q0.7720.99
111_R120_N0.7710.99
5_A8_A0.7640.99
61_E64_L0.7570.99
15_L98_I0.7560.99
66_L96_F0.7560.99
15_L23_R0.7560.99
234_M238_K0.7550.99
163_A166_N0.7540.99
84_R151_L0.7530.99
101_T106_G0.7510.99
182_A249_A0.7490.99
71_L119_L0.7470.99
134_S225_L0.7460.99
32_D35_R0.7450.99
68_R80_R0.7430.99
4_T8_A0.7420.99
184_D187_L0.7370.99
104_A107_P0.7370.99
176_A201_I0.7370.99
164_L168_L0.7330.99
64_L67_L0.7330.99
199_E247_A0.7320.99
6_A9_A0.7290.99
156_A216_D0.7280.99
37_R120_N0.7270.99
131_M225_L0.7260.99
101_T104_A0.7240.99
195_P263_Q0.7200.99
41_I190_N0.7180.99
35_R38_W0.7170.99
223_G231_A0.7130.99
34_E111_R0.7110.99
58_A62_A0.7100.99
34_E99_F0.7090.99
175_K202_P0.7070.99
64_L83_M0.7040.99
9_A12_N0.7040.99
223_G230_A0.7020.99
213_L220_E0.6950.99
184_D189_A0.6920.99
68_R73_A0.6890.99
14_F96_F0.6890.99
155_G158_E0.6880.99
141_A282_I0.6860.99
156_A159_D0.6860.99
162_R255_G0.6840.99
161_A246_F0.6810.99
226_P233_R0.6780.98
157_E224_R0.6750.98
62_A65_A0.6750.98
238_K241_L0.6740.98
41_I45_V0.6730.98
223_G227_D0.6720.98
13_A17_S0.6700.98
110_W121_F0.6680.98
166_N252_T0.6660.98
65_A69_A0.6650.98
101_T105_T0.6650.98
161_A260_Y0.6640.98
37_R41_I0.6600.98
116_H281_H0.6600.98
42_P47_R0.6590.98
223_G234_M0.6580.98
240_A244_T0.6570.98
217_L220_E0.6560.98
35_R42_P0.6560.98
84_R87_E0.6540.98
240_A243_K0.6540.98
139_N276_N0.6490.98
34_E48_K0.6490.98
37_R40_N0.6480.98
189_A194_A0.6460.98
52_L64_L0.6460.98
219_E222_V0.6450.98
71_L79_A0.6450.98
119_L134_S0.6440.98
186_I189_A0.6430.98
21_E25_K0.6400.98
30_F34_E0.6370.98
6_A10_A0.6370.98
26_A55_M0.6350.98
162_R251_G0.6350.98
85_L88_V0.6320.98
27_T98_I0.6310.98
265_P289_P0.6310.98
8_A101_T0.6310.98
131_M233_R0.6290.98
8_A11_A0.6250.97
103_S106_G0.6200.97
241_L264_G0.6180.97
66_L69_A0.6160.97
233_R237_I0.6160.97
288_D291_N0.6130.97
236_E240_A0.6110.97
142_E150_G0.6100.97
289_P293_F0.6090.97
37_R48_K0.6070.97
19_D59_Q0.6040.97
6_A226_P0.6030.97
86_E90_G0.6010.97
273_N279_A0.5960.97
219_E238_K0.5940.97
226_P229_L0.5940.97
178_L182_A0.5920.96
85_L138_A0.5910.96
73_A227_D0.5910.96
39_H46_E0.5890.96
183_P187_L0.5870.96
127_D232_A0.5750.96
48_K97_S0.5740.96
210_Q213_L0.5720.96
221_Y269_I0.5700.96
266_T288_D0.5690.96
266_T290_N0.5690.96
12_N104_A0.5680.96
115_H140_P0.5670.96
79_A83_M0.5610.95
49_G52_L0.5600.95
183_P186_I0.5570.95
157_E260_Y0.5560.95
39_H43_F0.5560.95
162_R253_E0.5540.95
146_G149_K0.5530.95
166_N251_G0.5530.95
209_A213_L0.5520.95
91_E146_G0.5510.95
31_D34_E0.5510.95
274_T279_A0.5500.95
144_R149_K0.5500.95
275_Q280_N0.5490.95
26_A50_L0.5480.95
171_E175_K0.5450.94
35_R40_N0.5450.94
36_R40_N0.5440.94
222_V233_R0.5440.94
143_V147_P0.5440.94
187_L190_N0.5430.94
22_Q25_K0.5430.94
184_D257_P0.5420.94
199_E245_H0.5410.94
103_S107_P0.5400.94
222_V230_A0.5400.94
77_E80_R0.5400.94
10_A69_A0.5390.94
188_T268_L0.5380.94
190_N194_A0.5380.94
184_D274_T0.5360.94
211_R241_L0.5340.94
131_M229_L0.5340.94
107_P125_D0.5320.94
51_P55_M0.5290.94
18_L22_Q0.5290.94
70_G226_P0.5280.93
55_M96_F0.5270.93
33_P36_R0.5260.93
161_A167_S0.5260.93
67_L96_F0.5260.93
258_H273_N0.5250.93
171_E205_E0.5240.93
215_L244_T0.5230.93
33_P37_R0.5230.93
47_R113_E0.5230.93
7_M69_A0.5230.93
106_G124_V0.5230.93
234_M237_I0.5210.93
86_E139_N0.5210.93
233_R291_N0.5190.93
8_A103_S0.5180.93
212_A238_K0.5170.93
273_N276_N0.5160.93
19_D56_T0.5160.93
216_D220_E0.5150.93
218_I267_F0.5150.93
276_N280_N0.5120.92
67_L76_Y0.5120.92
97_S113_E0.5110.92
204_S243_K0.5100.92
245_H263_Q0.5100.92
78_K156_A0.5090.92
35_R109_G0.5080.92
116_H283_H0.5080.92
275_Q282_I0.5070.92
160_L167_S0.5050.92
38_W49_G0.5050.92
271_Y282_I0.5030.92
236_E290_N0.5030.92
244_T264_G0.5030.92
110_W123_V0.5000.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3u65B 1 0.2881 41.4 0.96 Contact Map
2pfyA 1 0.2847 40.6 0.961 Contact Map
2hpgA 1 0.2881 38 0.961 Contact Map
4nguA 1 0.3017 37.5 0.961 Contact Map
4pe3A 1 0.3085 37.3 0.961 Contact Map
4pbqA 1 0.2915 35.3 0.962 Contact Map
4ovsA 1 0.2915 33.6 0.962 Contact Map
2pfzA 1 0.2983 28.7 0.964 Contact Map
4n8gA 3 0.3288 28.7 0.964 Contact Map
2zzvA 2 0.3356 25.1 0.965 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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