GREMLIN Database
DUF3464 - Protein of unknown function (DUF3464)
PFAM: PF11947 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 150 (138)
Sequences: 581 (414)
Seq/√Len: 35.3
META: 0.566

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
71_T108_G5.2801.00
117_L142_M4.1461.00
84_L89_I3.8251.00
84_L90_A3.3831.00
53_E57_N2.9321.00
77_V104_F2.7591.00
100_V104_F2.2140.99
125_R129_L2.1590.99
77_V81_S2.1420.99
103_G110_L2.1040.99
98_L104_F2.0740.99
18_P21_K1.9900.98
125_R128_S1.9780.98
33_N38_S1.8410.97
60_A67_C1.7470.96
75_M79_V1.7120.95
79_V101_S1.6920.95
102_M109_L1.6670.95
103_G107_L1.6650.95
98_L102_M1.6460.94
75_M105_F1.6410.94
25_K28_K1.5520.92
137_T141_R1.5500.92
96_A100_V1.5160.91
60_A75_M1.5090.91
53_E78_F1.5080.91
78_F97_V1.4590.90
21_K27_S1.4480.89
22_K28_K1.4380.89
16_K19_K1.3880.87
28_K31_S1.3720.87
36_K40_K1.3510.86
144_A148_E1.3470.86
101_S104_F1.3440.86
132_W136_K1.3230.85
61_R84_L1.2980.84
15_K20_K1.2860.83
13_K19_K1.2740.82
137_T142_M1.2700.82
56_S79_V1.2330.80
54_V124_E1.2180.79
76_S80_V1.2180.79
82_Y101_S1.2170.79
23_K26_K1.2100.79
139_W143_K1.1930.78
11_K18_P1.1880.78
87_R107_L1.1710.77
74_G105_F1.1580.76
107_L141_R1.1270.74
17_K21_K1.1170.73
29_K32_A1.1060.72
74_G93_P1.0990.72
73_L107_L1.0980.72
16_K20_K1.0960.72
56_S141_R1.0920.71
65_F129_L1.0910.71
17_K25_K1.0890.71
59_M105_F1.0890.71
45_K48_S1.0600.69
102_M145_A1.0490.68
75_M99_L1.0480.68
78_F112_L1.0410.68
94_P113_S1.0390.68
134_E137_T1.0380.67
18_P22_K1.0320.67
63_M112_L1.0200.66
66_F135_F1.0040.65
97_V135_F0.9880.64
89_I112_L0.9820.63
120_S127_G0.9710.62
37_K40_K0.9590.61
82_Y115_G0.9560.61
17_K24_P0.9530.61
82_Y113_S0.9450.60
91_E144_A0.9450.60
104_F112_L0.9420.60
80_V124_E0.9420.60
57_N113_S0.9390.60
81_S98_L0.9280.59
65_F83_Y0.9240.58
101_S113_S0.9180.58
97_V102_M0.9140.58
114_Y118_S0.9110.57
64_A108_G0.9100.57
106_G146_W0.9060.57
124_E133_E0.9030.57
56_S82_Y0.9030.57
24_P30_A0.9020.56
56_S109_L0.8950.56
55_V119_A0.8940.56
35_E38_S0.8930.56
19_K22_K0.8920.56
137_T140_G0.8920.56
128_S134_E0.8830.55
13_K18_P0.8820.55
49_S116_I0.8730.54
122_D145_A0.8720.54
133_E136_K0.8670.54
114_Y124_E0.8660.53
51_I82_Y0.8630.53
79_V104_F0.8560.53
112_L145_A0.8530.52
69_I124_E0.8510.52
61_R129_L0.8350.51
86_T92_L0.8330.51
81_S126_P0.8310.50
53_E94_P0.8260.50
67_C105_F0.8220.50
19_K26_K0.8110.49
102_M134_E0.8040.48
37_K46_R0.8040.48
28_K32_A0.8030.48
60_A64_A0.7980.48
74_G106_G0.7930.47
24_P29_K0.7790.46
56_S59_M0.7760.46
23_K43_K0.7760.46
37_K41_K0.7710.45
122_D125_R0.7570.44
37_K42_S0.7560.44
64_A137_T0.7500.43
48_S90_A0.7490.43
60_A71_T0.7470.43
27_S38_S0.7460.43
11_K23_K0.7450.43
50_A116_I0.7450.43
27_S30_A0.7380.42
21_K29_K0.7260.41
56_S135_F0.7260.41
135_F139_W0.7250.41
31_S37_K0.7180.41
61_R119_A0.7130.40
44_P60_A0.7130.40
19_K25_K0.7110.40
88_N137_T0.7100.40
100_V146_W0.7090.40
17_K20_K0.7070.40
20_K27_S0.7010.39
25_K29_K0.7010.39
23_K27_S0.6920.39
65_F113_S0.6820.38
113_S117_L0.6790.38
69_I78_F0.6760.37
38_S41_K0.6740.37
93_P113_S0.6690.37
22_K26_K0.6650.36
26_K30_A0.6630.36
18_P26_K0.6570.36
13_K16_K0.6540.36
75_M101_S0.6540.36
17_K29_K0.6520.35
117_L143_K0.6480.35
14_R20_K0.6460.35
128_S143_K0.6450.35
18_P25_K0.6400.34
36_K144_A0.6360.34
52_P138_N0.6350.34
71_T77_V0.6290.34
92_L116_I0.6270.33
94_P101_S0.6250.33
26_K29_K0.6210.33
18_P24_P0.6210.33
67_C135_F0.6200.33
15_K18_P0.6190.33
101_S112_L0.6190.33
77_V116_I0.6140.33
83_Y122_D0.6100.32
63_M81_S0.6040.32
41_K45_K0.5990.31
20_K24_P0.5980.31
42_S46_R0.5950.31
52_P58_R0.5900.31
66_F116_I0.5820.30
66_F97_V0.5750.30
55_V58_R0.5750.30
32_A37_K0.5730.29
29_K33_N0.5710.29
18_P29_K0.5680.29
84_L88_N0.5670.29
87_R94_P0.5660.29
104_F126_P0.5630.29
51_I59_M0.5620.29
64_A101_S0.5500.28
127_G131_G0.5480.28
102_M136_K0.5450.28
33_N37_K0.5430.27
85_V92_L0.5410.27
143_K146_W0.5350.27
128_S133_E0.5330.27
59_M141_R0.5320.27
81_S104_F0.5320.27
49_S145_A0.5310.27
79_V140_G0.5300.26
12_K16_K0.5300.26
24_P28_K0.5230.26
81_S85_V0.5220.26
55_V134_E0.5210.26
58_R138_N0.5210.26
56_S77_V0.5210.26
21_K28_K0.5180.26
18_P28_K0.5150.26
11_K16_K0.5130.25
21_K26_K0.5130.25
134_E138_N0.5110.25
99_L119_A0.5080.25
112_L141_R0.5050.25
17_K23_K0.5000.25
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1rh5C 1 0.2133 8.9 0.957 Contact Map
4ldsA 2 0.9667 8.5 0.957 Contact Map
3effK 4 0.6067 6.7 0.959 Contact Map
4ogqF 1 0.2133 5.9 0.96 Contact Map
3k6gD 1 0.24 5.3 0.961 Contact Map
2clyA 1 0.2133 4.6 0.962 Contact Map
3wu2J 1 0.2533 4.5 0.962 Contact Map
3wdoA 1 0.4 4.2 0.963 Contact Map
1qb3A 2 0.1733 3.7 0.964 Contact Map
3v5uA 1 0.36 3.5 0.964 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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