GREMLIN Database
DUF3445 - Protein of unknown function (DUF3445)
PFAM: PF11927 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 228 (223)
Sequences: 3033 (2262)
Seq/√Len: 151.4
META: 0.661

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
96_Q105_T3.8531.00
50_L214_L3.7981.00
24_E27_R3.1711.00
27_R31_E3.0491.00
203_T216_T2.7361.00
38_A121_I2.6951.00
43_A47_A2.5941.00
25_K108_V2.5551.00
85_G108_V2.5431.00
88_V92_L2.4061.00
18_Y66_F2.3321.00
116_S119_E2.2821.00
92_L214_L2.2651.00
126_A140_A2.2231.00
32_R35_E2.2061.00
93_C109_L2.1341.00
52_E209_R2.1231.00
26_R86_R2.1111.00
113_S219_T2.0891.00
93_C217_I2.0761.00
40_L121_I2.0641.00
223_P226_E1.9371.00
98_R103_R1.9251.00
33_P82_E1.9051.00
39_A106_A1.8661.00
135_Y143_V1.8531.00
166_D171_Q1.8131.00
150_L213_I1.7901.00
24_E168_R1.7831.00
12_L54_L1.7821.00
50_L92_L1.7581.00
193_E225_A1.7141.00
6_L155_P1.7131.00
97_R148_D1.6841.00
98_R101_E1.6631.00
150_L204_L1.6611.00
47_A106_A1.6611.00
28_L36_V1.6581.00
84_A92_L1.6311.00
96_Q212_A1.6121.00
39_A43_A1.6101.00
104_L128_I1.5961.00
93_C104_L1.5931.00
159_F199_V1.5731.00
70_D73_R1.5721.00
67_R75_R1.5681.00
123_R127_E1.5661.00
93_C107_G1.5621.00
114_S164_Y1.4981.00
56_E60_A1.4931.00
24_E111_F1.4851.00
40_L122_G1.4831.00
156_V204_L1.4701.00
15_D89_Q1.4681.00
37_F82_E1.4661.00
82_E108_V1.4491.00
43_A106_A1.4471.00
59_P62_Y1.4451.00
57_H61_R1.4381.00
102_Y147_F1.4351.00
124_P127_E1.4221.00
151_Q154_R1.4141.00
6_L157_W1.4131.00
18_Y22_L1.4101.00
13_E220_Y1.4021.00
126_A144_D1.3991.00
35_E170_F1.3761.00
67_R73_R1.3661.00
17_H21_E1.3661.00
125_L147_F1.3561.00
39_A44_E1.3421.00
71_G74_N1.3331.00
32_R170_F1.3091.00
39_A47_A1.3021.00
14_V18_Y1.2961.00
163_L195_L1.2861.00
46_A212_A1.2681.00
125_L143_V1.2621.00
21_E89_Q1.2571.00
44_E48_W1.2541.00
7_D10_D1.2431.00
102_Y148_D1.2281.00
49_E210_S1.2211.00
109_L117_L1.2131.00
135_Y140_A1.1961.00
29_L108_V1.1511.00
35_E118_A1.1481.00
48_W52_E1.1411.00
94_L212_A1.1311.00
114_S173_P1.1291.00
135_Y144_D1.1050.99
43_A96_Q1.1000.99
29_L33_P1.0950.99
114_S171_Q1.0880.99
53_L207_L1.0800.99
113_S164_Y1.0780.99
102_Y144_D1.0760.99
94_L214_L1.0750.99
62_Y66_F1.0730.99
73_R76_L1.0710.99
38_A118_A1.0690.99
22_L25_K1.0640.99
50_L207_L1.0640.99
33_P37_F1.0630.99
71_G76_L1.0450.99
25_K85_G1.0400.99
93_C121_I1.0380.99
45_A96_Q1.0370.99
82_E86_R1.0340.99
103_R124_P1.0320.99
105_T212_A1.0260.99
51_L87_L1.0110.99
142_R146_F1.0010.99
97_R102_Y1.0000.99
104_L107_G0.9950.99
97_R100_G0.9940.99
54_L84_A0.9920.99
191_A194_R0.9900.99
11_W205_R0.9820.99
17_H20_A0.9780.99
35_E116_S0.9740.99
139_L142_R0.9580.98
53_L205_R0.9550.98
164_Y171_Q0.9550.98
19_A23_A0.9500.98
74_N77_T0.9500.98
101_E124_P0.9490.98
92_L216_T0.9490.98
57_H62_Y0.9490.98
99_D103_R0.9430.98
102_Y206_R0.9400.98
107_G117_L0.9360.98
104_L202_Q0.9350.98
81_L106_A0.9310.98
153_G205_R0.9280.98
111_F219_T0.9260.98
22_L26_R0.9250.98
70_D74_N0.9250.98
152_P155_P0.9120.98
29_L37_F0.9110.98
29_L82_E0.8980.98
82_E85_G0.8970.98
120_K128_I0.8960.98
143_V147_F0.8940.97
167_P196_F0.8910.97
164_Y221_L0.8870.97
51_L84_A0.8810.97
40_L105_T0.8800.97
187_T190_N0.8790.97
113_S160_N0.8750.97
185_R188_A0.8740.97
54_L92_L0.8730.97
18_Y89_Q0.8660.97
138_K142_R0.8650.97
90_E216_T0.8640.97
101_E144_D0.8630.97
58_L88_V0.8630.97
50_L81_L0.8610.97
145_R149_R0.8600.97
59_P68_L0.8440.96
47_A94_L0.8350.96
222_D226_E0.8340.96
178_Y181_W0.8320.96
146_F149_R0.8280.96
206_R211_G0.8270.96
125_L213_I0.8260.96
97_R211_G0.8250.96
3_L146_F0.8250.96
55_L83_T0.8220.96
52_E56_E0.8180.96
14_V90_E0.8150.96
66_F74_N0.8100.96
49_E208_P0.8070.95
34_D38_A0.8020.95
184_A187_T0.7980.95
147_F204_L0.7940.95
98_R105_T0.7890.95
36_V110_C0.7880.95
8_P155_P0.7860.95
25_K82_E0.7810.95
70_D75_R0.7800.94
136_D140_A0.7790.94
37_F81_L0.7770.94
51_L80_P0.7700.94
47_A84_A0.7690.94
53_L56_E0.7680.94
135_Y139_L0.7650.94
44_E80_P0.7640.94
120_K131_P0.7620.94
204_L213_I0.7580.94
124_P128_I0.7570.94
199_V220_Y0.7550.93
170_F173_P0.7510.93
55_L87_L0.7510.93
15_D218_R0.7510.93
75_R78_G0.7380.93
29_L86_R0.7340.93
55_L59_P0.7320.92
146_F156_V0.7310.92
28_L170_F0.7300.92
37_F108_V0.7230.92
195_L224_L0.7230.92
46_A106_A0.7200.92
95_L128_I0.7200.92
112_P115_W0.7190.92
15_D21_E0.7190.92
14_V66_F0.7160.92
72_L87_L0.7140.91
152_P206_R0.7040.91
59_P63_P0.7030.91
63_P66_F0.7010.91
133_P161_W0.6990.91
113_S162_S0.6970.90
144_D148_D0.6900.90
65_L76_L0.6890.90
159_F220_Y0.6870.90
179_D182_G0.6860.90
204_L216_T0.6820.90
207_L214_L0.6800.89
115_W120_K0.6780.89
155_P205_R0.6760.89
40_L43_A0.6690.89
70_D76_L0.6570.88
150_L206_R0.6530.87
15_D18_Y0.6530.87
26_R79_E0.6500.87
46_A96_Q0.6500.87
47_A51_L0.6490.87
166_D221_L0.6490.87
53_L57_H0.6480.87
43_A105_T0.6470.87
47_A80_P0.6410.86
146_F206_R0.6380.86
4_R157_W0.6280.85
55_L72_L0.6250.85
186_V190_N0.6250.85
151_Q211_G0.6240.85
157_W201_R0.6240.85
192_G224_L0.6230.85
26_R30_A0.6200.85
161_W199_V0.6190.84
162_S199_V0.6180.84
105_T122_G0.6160.84
24_E28_L0.6160.84
127_E130_A0.6160.84
89_Q218_R0.6080.83
50_L88_V0.6050.83
114_S162_S0.6040.83
95_L105_T0.6030.83
49_E207_L0.6020.83
8_P205_R0.6020.83
14_V89_Q0.6010.83
85_G92_L0.6000.83
13_E199_V0.5990.83
199_V222_D0.5990.83
83_T86_R0.5980.82
32_R36_V0.5950.82
167_P221_L0.5940.82
19_A76_L0.5920.82
202_Q207_L0.5920.82
95_L102_Y0.5910.82
37_F47_A0.5910.82
20_A167_P0.5900.82
23_A27_R0.5890.81
59_P66_F0.5880.81
59_P65_L0.5870.81
49_E152_P0.5860.81
149_R153_G0.5850.81
5_P154_R0.5840.81
21_E25_K0.5840.81
42_G96_Q0.5820.81
5_P156_V0.5820.81
73_R78_G0.5790.80
72_L76_L0.5760.80
61_R66_F0.5760.80
56_E209_R0.5760.80
135_Y142_R0.5740.80
55_L84_A0.5720.80
90_E203_T0.5710.79
190_N194_R0.5710.79
37_F84_A0.5700.79
41_P44_E0.5690.79
142_R149_R0.5680.79
22_L85_G0.5660.79
44_E47_A0.5660.79
165_D195_L0.5660.79
166_D194_R0.5640.79
159_F201_R0.5620.78
95_L147_F0.5590.78
223_P227_L0.5580.78
205_R216_T0.5580.78
116_S173_P0.5580.78
45_A210_S0.5570.78
85_G90_E0.5570.78
126_A143_V0.5550.78
141_R145_R0.5530.77
68_L72_L0.5510.77
162_S171_Q0.5500.77
75_R79_E0.5480.77
74_N78_G0.5440.76
89_Q111_F0.5410.76
36_V170_F0.5400.76
114_S142_R0.5400.76
18_Y74_N0.5390.75
104_L117_L0.5370.75
178_Y182_G0.5370.75
167_P171_Q0.5360.75
190_N193_E0.5330.75
115_W217_I0.5330.75
17_H89_Q0.5330.75
14_V58_L0.5280.74
149_R154_R0.5280.74
101_E127_E0.5270.74
2_G5_P0.5240.73
197_L222_D0.5230.73
89_Q220_Y0.5190.73
162_S166_D0.5180.73
49_E211_G0.5170.72
8_P57_H0.5170.72
3_L161_W0.5160.72
72_L77_T0.5150.72
28_L111_F0.5140.72
10_D13_E0.5110.72
133_P162_S0.5090.71
58_L62_Y0.5080.71
206_R213_I0.5070.71
54_L59_P0.5060.71
25_K111_F0.5050.71
94_L106_A0.5040.71
54_L88_V0.5040.71
14_V61_R0.5030.70
18_Y65_L0.5020.70
31_E34_D0.5020.70
80_P117_L0.5000.70
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2p4gA 2 0.3289 19.5 0.958 Contact Map
4ha7A 2 0.2939 14.8 0.96 Contact Map
3jy6A 2 0.6228 10.3 0.963 Contact Map
3gybA 2 0.6184 9.6 0.963 Contact Map
4jenA 2 0.5044 6.3 0.966 Contact Map
2y3aB 1 0.4561 6.1 0.966 Contact Map
2aznA 2 0.2632 5.7 0.967 Contact Map
2gj3A 7 0.5263 5.2 0.967 Contact Map
3clkA 2 0.614 5.1 0.967 Contact Map
3k9cA 2 0.614 5 0.968 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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