GREMLIN Database
DUF3431 - Protein of unknown function (DUF3431)
PFAM: PF11913 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 214 (201)
Sequences: 1524 (1311)
Seq/√Len: 92.5
META: 0.689

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
58_D166_K4.6931.00
61_P64_T4.6501.00
166_K170_E4.2461.00
53_I161_I3.5691.00
52_Y61_P3.5261.00
51_T55_D3.2891.00
3_V64_T3.2681.00
54_I173_R3.1651.00
64_T161_I3.0251.00
158_R162_L2.6631.00
160_R163_K2.5121.00
6_R28_N2.5021.00
68_H71_P2.4301.00
56_N59_N2.3971.00
52_Y64_T2.3791.00
174_D178_D2.3781.00
170_E174_D2.3041.00
187_G191_E2.2911.00
47_H173_R2.2511.00
188_R192_R2.1741.00
167_E170_E2.1431.00
164_R168_F2.1041.00
4_V15_L2.0831.00
10_E69_G2.0321.00
172_L190_F2.0281.00
51_T173_R1.9691.00
50_L194_W1.9431.00
29_K48_T1.9121.00
62_D158_R1.9061.00
44_R183_D1.8881.00
49_Y152_A1.8611.00
12_L15_L1.8161.00
156_V198_F1.7921.00
45_E152_A1.7001.00
71_P82_D1.6961.00
47_H51_T1.6371.00
50_L54_I1.5731.00
52_Y55_D1.5441.00
164_R169_Y1.5281.00
54_I166_K1.5031.00
46_A187_G1.4971.00
6_R12_L1.4901.00
58_D162_L1.4741.00
63_V155_A1.4461.00
45_E49_Y1.4100.99
196_Y199_G1.3770.99
68_H74_W1.3620.99
169_Y198_F1.3540.99
46_A190_F1.3490.99
184_P188_R1.3270.99
157_S160_R1.3250.99
13_S16_K1.2860.99
130_L133_A1.2830.99
44_R187_G1.2820.99
2_L7_Y1.2820.99
50_L172_L1.2770.99
174_D177_L1.2770.99
83_N87_L1.2650.99
188_R193_L1.2610.99
70_H73_A1.2570.99
27_Y30_D1.2380.98
190_F194_W1.2290.98
168_F171_R1.2240.98
54_I170_E1.2110.98
26_I29_K1.2060.98
188_R191_E1.2050.98
94_Y98_E1.2050.98
15_L24_V1.1870.98
12_L69_G1.1700.98
63_V157_S1.1580.98
3_V7_Y1.1550.98
47_H177_L1.1480.97
27_Y52_Y1.1190.97
129_P134_W1.1120.97
183_D187_G1.1040.97
100_Y104_R1.0970.97
25_I29_K1.0870.96
104_R110_G1.0830.96
154_F157_S1.0760.96
71_P74_W1.0630.96
53_I56_N1.0630.96
4_V67_L1.0500.96
42_K176_L1.0450.96
148_F151_C1.0400.95
45_E50_L1.0330.95
190_F197_I1.0310.95
99_G104_R1.0300.95
53_I66_F1.0270.95
24_V29_K1.0260.95
165_P168_F1.0250.95
45_E68_H1.0120.95
179_T186_S1.0080.95
85_E89_R1.0080.95
31_D34_A1.0070.95
164_R197_I1.0050.94
129_P133_A1.0030.94
55_D173_R1.0010.94
83_N86_L0.9990.94
85_E88_R0.9890.94
173_R177_L0.9880.94
193_L196_Y0.9870.94
28_N31_D0.9710.93
130_L134_W0.9690.93
168_F172_L0.9650.93
84_V88_R0.9600.93
105_C110_G0.9490.93
73_A76_N0.9390.92
66_F154_F0.9290.92
184_P192_R0.9290.92
146_I196_Y0.9120.91
63_V96_L0.9080.91
99_G105_C0.8950.90
2_L6_R0.8930.90
25_I28_N0.8920.90
67_L153_Q0.8870.90
105_C111_C0.8860.90
164_R198_F0.8850.90
102_N105_C0.8810.90
50_L169_Y0.8800.90
166_K169_Y0.8710.89
131_A135_E0.8600.88
42_K47_H0.8560.88
156_V161_I0.8540.88
5_A29_K0.8500.88
183_D188_R0.8490.88
48_T52_Y0.8450.88
22_W65_V0.8390.87
156_V197_I0.8360.87
2_L18_L0.8340.87
104_R109_P0.8310.87
44_R68_H0.8280.86
46_A50_L0.8250.86
132_E136_E0.8220.86
39_P47_H0.8110.85
67_L83_N0.8050.85
181_L186_S0.8040.85
189_V194_W0.8010.85
129_P132_E0.7960.84
161_I169_Y0.7960.84
184_P187_G0.7950.84
57_Y60_L0.7930.84
53_I166_K0.7930.84
87_L90_L0.7920.84
133_A138_F0.7790.83
68_H73_A0.7710.82
6_R10_E0.7690.82
7_Y12_L0.7690.82
157_S162_L0.7690.82
183_D186_S0.7600.81
18_L21_D0.7590.81
47_H52_Y0.7540.81
132_E135_E0.7470.80
99_G111_C0.7460.80
93_D97_R0.7420.80
92_L95_V0.7410.80
191_E197_I0.7410.80
4_V24_V0.7390.80
25_I56_N0.7330.79
133_A189_V0.7320.79
135_E141_D0.7290.79
12_L26_I0.7270.78
7_Y28_N0.7260.78
91_N96_L0.7250.78
186_S192_R0.7230.78
103_L107_W0.7190.78
62_D159_E0.7180.78
25_I52_Y0.7170.77
78_D87_L0.7160.77
107_W110_G0.7150.77
2_L67_L0.7140.77
175_W186_S0.7080.77
185_I189_V0.7030.76
9_N12_L0.7020.76
95_V99_G0.6990.76
153_Q157_S0.6960.75
94_Y97_R0.6960.75
46_A194_W0.6890.75
182_D187_G0.6850.74
36_L39_P0.6840.74
82_D86_L0.6830.74
22_W25_I0.6820.74
8_Y31_D0.6820.74
150_C153_Q0.6800.74
165_P170_E0.6790.74
189_V193_L0.6790.74
5_A48_T0.6790.74
122_E128_L0.6790.74
95_V98_E0.6780.73
114_E126_E0.6750.73
80_D87_L0.6740.73
176_L188_R0.6730.73
44_R47_H0.6700.73
81_Y84_V0.6700.73
5_A8_Y0.6640.72
49_Y154_F0.6640.72
125_P128_L0.6630.72
42_K179_T0.6590.71
87_L91_N0.6530.71
134_W143_P0.6530.71
68_H188_R0.6520.71
194_W198_F0.6500.70
44_R48_T0.6500.70
176_L187_G0.6480.70
134_W138_F0.6460.70
45_E194_W0.6450.70
100_Y105_C0.6410.69
29_K52_Y0.6380.69
160_R200_G0.6340.68
46_A148_F0.6330.68
80_D83_N0.6310.68
86_L94_Y0.6310.68
113_D116_H0.6300.68
45_E187_G0.6250.67
81_Y90_L0.6220.67
64_T157_S0.6220.67
92_L96_L0.6200.67
89_R94_Y0.6150.66
42_K177_L0.6120.66
40_P177_L0.6090.65
168_F197_I0.6090.65
99_G103_L0.6060.65
34_A37_H0.6060.65
81_Y87_L0.6040.65
133_A137_L0.6040.65
176_L179_T0.6020.64
24_V28_N0.6020.64
15_L18_L0.6010.64
185_I188_R0.6000.64
130_L137_L0.6000.64
31_D37_H0.5950.64
152_A160_R0.5950.64
3_V52_Y0.5940.63
98_E101_V0.5930.63
87_L103_L0.5900.63
48_T68_H0.5900.63
102_N151_C0.5880.63
23_N26_I0.5870.62
40_P47_H0.5840.62
70_H76_N0.5820.62
186_S189_V0.5820.62
68_H153_Q0.5780.61
60_L162_L0.5750.61
125_P133_A0.5750.61
80_D84_V0.5740.61
109_P157_S0.5730.61
146_I189_V0.5720.60
154_F169_Y0.5710.60
131_A146_I0.5710.60
47_H68_H0.5700.60
171_R197_I0.5650.60
49_Y75_H0.5630.59
82_D85_E0.5620.59
41_N177_L0.5620.59
5_A10_E0.5620.59
128_L133_A0.5580.59
34_A38_V0.5530.58
33_S37_H0.5530.58
105_C109_P0.5490.57
156_V160_R0.5490.57
40_P69_G0.5480.57
190_F193_L0.5470.57
30_D192_R0.5470.57
104_R107_W0.5470.57
191_E194_W0.5440.57
13_S17_E0.5430.57
114_E118_W0.5380.56
172_L194_W0.5380.56
123_G126_E0.5370.56
2_L22_W0.5370.56
63_V95_V0.5370.56
46_A188_R0.5350.55
179_T188_R0.5340.55
5_A77_D0.5270.54
131_A134_W0.5250.54
177_L180_P0.5240.54
72_F76_N0.5240.54
71_P153_Q0.5220.54
9_N30_D0.5200.53
65_V155_A0.5200.53
46_A152_A0.5180.53
149_P154_F0.5170.53
35_P39_P0.5170.53
189_V195_H0.5170.53
139_P163_K0.5140.53
26_I30_D0.5140.53
57_Y162_L0.5120.52
113_D118_W0.5120.52
78_D81_Y0.5110.52
96_L101_V0.5090.52
163_K169_Y0.5090.52
154_F182_D0.5080.52
68_H72_F0.5080.52
137_L140_G0.5070.52
90_L93_D0.5060.51
90_L95_V0.5050.51
39_P52_Y0.5030.51
72_F78_D0.5030.51
26_I171_R0.5020.51
102_N111_C0.5020.51
86_L89_R0.5020.51
136_E178_D0.5010.51
99_G102_N0.5000.51
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2a6hD 1 0.2804 6 0.959 Contact Map
4p0gA 1 0.3084 5.2 0.961 Contact Map
2ar5A 1 0.3318 5.1 0.961 Contact Map
1hqmD 1 0.1355 3.9 0.963 Contact Map
3ee7A 3 0.3271 3.3 0.964 Contact Map
4p2iA 1 0.3178 3.1 0.964 Contact Map
3nymA 2 0.1963 2.6 0.966 Contact Map
4hasA 1 0.3224 2.1 0.968 Contact Map
4hjzA 1 0.2757 2 0.968 Contact Map
2i4kA 1 0.3178 1.8 0.969 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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