GREMLIN Database
DUF3427 - Domain of unknown function (DUF3427)
PFAM: PF11907 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 284 (279)
Sequences: 1799 (1507)
Seq/√Len: 90.2
META: 0.633

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
190_S193_L4.9001.00
12_P112_L4.3491.00
15_L111_G4.0751.00
245_D272_E3.9871.00
179_S182_T3.7201.00
221_R243_E3.4721.00
223_H240_Y3.3911.00
243_E272_E3.3181.00
2_L104_L3.2171.00
153_S156_G3.0831.00
170_V244_V2.8861.00
187_Y195_H2.7991.00
19_K112_L2.7821.00
256_M264_P2.7341.00
210_Q214_N2.6931.00
214_N217_E2.6071.00
199_K208_E2.5191.00
224_L244_V2.4631.00
170_V194_F2.3281.00
101_K105_L2.3201.00
17_I32_L2.2511.00
33_K46_E2.1691.00
149_N152_S2.1141.00
197_Q266_E2.0721.00
45_S48_T2.0041.00
88_S91_F1.9861.00
216_K245_D1.9471.00
194_F224_L1.9321.00
2_L6_L1.8901.00
17_I52_A1.8351.00
195_H266_E1.8331.00
244_V269_W1.8291.00
226_V241_L1.8141.00
188_F275_V1.8141.00
246_P249_H1.7901.00
178_I182_T1.7751.00
200_S256_M1.7631.00
19_K109_E1.7601.00
213_I251_A1.7481.00
15_L112_L1.7411.00
93_E96_K1.7311.00
54_N59_E1.6941.00
192_S270_K1.6871.00
160_Y220_I1.6841.00
247_V270_K1.6751.00
141_C155_I1.6641.00
180_E183_K1.6451.00
198_S209_V1.6161.00
202_R209_V1.6041.00
178_I183_K1.6011.00
248_S268_T1.5971.00
150_E159_K1.5931.00
202_R208_E1.5801.00
144_L167_P1.5591.00
224_L271_L1.5581.00
20_L35_E1.5551.00
192_S274_P1.5361.00
178_I181_S1.5251.00
209_V265_V1.5231.00
156_G199_K1.5001.00
70_G73_P1.4801.00
158_Y199_K1.4791.00
134_K239_Y1.4761.00
152_S156_G1.4600.99
203_T263_K1.4540.99
161_D164_T1.4530.99
19_K23_E1.4520.99
20_L24_N1.4460.99
32_L52_A1.4430.99
241_L275_V1.4290.99
198_S202_R1.4010.99
276_P279_L1.3930.99
182_T200_S1.3530.99
6_L55_V1.3120.99
5_E138_K1.3060.99
22_L105_L1.2910.99
19_K105_L1.2890.99
193_L268_T1.2810.99
31_E34_K1.2770.99
130_K133_Q1.2630.99
227_R235_G1.2520.98
7_L15_L1.2430.98
72_C76_E1.2410.98
212_I222_I1.2410.98
202_R206_S1.2300.98
39_K116_E1.2230.98
166_C212_I1.2220.98
256_M259_T1.2100.98
205_D253_E1.2010.98
214_N218_N1.2010.98
211_R218_N1.1870.98
255_T258_N1.1870.98
229_N238_F1.1820.98
174_K186_D1.1790.98
73_P76_E1.1630.97
185_E197_Q1.1500.97
246_P251_A1.1500.97
215_H246_P1.1460.97
48_T52_A1.1440.97
196_W212_I1.1380.97
76_E86_S1.1380.97
168_I222_I1.1330.97
91_F94_L1.1270.97
81_E86_S1.1210.97
204_L265_V1.1190.97
166_C222_I1.1190.97
6_L15_L1.1180.97
241_L244_V1.1170.97
72_C75_I1.1130.97
140_V169_F1.1060.97
118_Y133_Q1.0970.96
141_C150_E1.0950.96
213_I267_I1.0940.96
87_L92_K1.0920.96
223_H243_E1.0850.96
51_S56_L1.0820.96
49_I52_A1.0760.96
166_C220_I1.0750.96
95_L105_L1.0640.96
46_E50_E1.0580.96
193_L270_K1.0560.95
36_L42_Y1.0330.95
160_Y166_C1.0290.95
26_S84_D1.0270.95
141_C172_Y1.0260.95
180_E259_T1.0190.95
89_E93_E1.0160.94
168_I244_V1.0150.94
70_G76_E1.0130.94
181_S200_S1.0110.94
137_R171_T1.0100.94
108_I112_L1.0080.94
210_Q213_I1.0020.94
245_D270_K1.0000.94
87_L91_F0.9980.94
167_P172_Y0.9950.94
141_C146_W0.9830.93
204_L253_E0.9800.93
150_E153_S0.9800.93
201_N263_K0.9770.93
44_V52_A0.9680.93
205_D210_Q0.9640.93
207_P210_Q0.9620.93
147_E163_D0.9600.93
13_H36_L0.9580.92
19_K39_K0.9580.92
114_R118_Y0.9550.92
20_L23_E0.9470.92
91_F104_L0.9450.92
171_T232_D0.9430.92
20_L27_I0.9420.92
7_L107_L0.9390.92
198_S208_E0.9380.92
233_G237_D0.9350.92
44_V49_I0.9290.91
211_R220_I0.9220.91
244_V271_L0.9200.91
88_S94_L0.9160.91
212_I269_W0.9150.91
16_L19_K0.9120.91
13_H48_T0.9120.91
129_F143_L0.9120.91
258_N262_K0.9090.90
255_T263_K0.9090.90
209_V267_I0.9050.90
215_H219_G0.9010.90
37_K43_E0.8920.90
11_R14_E0.8910.89
154_N199_K0.8850.89
2_L7_L0.8840.89
52_A56_L0.8790.89
119_Y130_K0.8770.89
195_H268_T0.8770.89
114_R224_L0.8760.89
35_E39_K0.8740.89
253_E256_M0.8710.88
60_F141_C0.8680.88
158_Y167_P0.8650.88
30_E33_K0.8640.88
105_L109_E0.8620.88
52_A61_F0.8580.88
48_T51_S0.8570.88
55_V60_F0.8540.87
209_V213_I0.8520.87
7_L110_Y0.8500.87
106_D109_E0.8500.87
199_K202_R0.8480.87
136_S233_G0.8470.87
137_R229_N0.8470.87
141_C154_N0.8440.87
63_E68_K0.8410.87
132_Y279_L0.8390.86
188_F194_F0.8360.86
159_K199_K0.8360.86
34_K37_K0.8350.86
206_S210_Q0.8350.86
2_L107_L0.8330.86
277_E281_D0.8320.86
196_W199_K0.8270.86
151_V154_N0.8250.86
203_T207_P0.8220.85
53_L85_Y0.8150.85
34_K38_K0.8130.85
21_L87_L0.8120.85
24_N31_E0.8110.85
194_F244_V0.8100.84
28_S84_D0.8070.84
17_I20_L0.8050.84
238_F241_L0.8040.84
222_I269_W0.8030.84
258_N261_G0.8020.84
22_L108_I0.8010.84
115_Y119_Y0.7980.84
253_E263_K0.7980.84
141_C156_G0.7960.83
29_I85_Y0.7950.83
15_L108_I0.7920.83
71_D76_E0.7900.83
150_E154_N0.7890.83
280_Y283_L0.7850.83
147_E162_K0.7830.82
226_V275_V0.7820.82
22_L25_G0.7820.82
222_I244_V0.7810.82
189_I195_H0.7810.82
29_I33_K0.7790.82
129_F244_V0.7770.82
62_T74_L0.7710.81
71_D74_L0.7700.81
171_T199_K0.7690.81
81_E84_D0.7650.81
27_I32_L0.7640.81
254_T266_E0.7630.81
171_T227_R0.7620.81
30_E34_K0.7590.80
169_F238_F0.7590.80
171_T230_K0.7570.80
9_G15_L0.7560.80
154_N159_K0.7520.80
146_W159_K0.7500.80
110_Y114_R0.7460.79
115_Y118_Y0.7370.78
209_V251_A0.7370.78
227_R232_D0.7300.78
122_E125_G0.7290.78
173_H231_D0.7290.78
57_N61_F0.7260.77
12_P15_L0.7240.77
188_F224_L0.7220.77
181_S198_S0.7190.77
186_D197_Q0.7150.76
99_E103_E0.7140.76
113_E116_E0.7060.75
35_E38_K0.7050.75
153_S159_K0.7050.75
137_R141_C0.7000.75
142_R158_Y0.7000.75
207_P211_R0.7000.75
173_H212_I0.6990.75
25_G34_K0.6940.74
71_D75_I0.6930.74
166_C211_R0.6930.74
9_G172_Y0.6920.74
64_S68_K0.6910.74
155_I158_Y0.6900.74
178_I238_F0.6890.74
179_S183_K0.6820.73
141_C153_S0.6760.72
27_I31_E0.6710.72
182_T186_D0.6690.71
153_S199_K0.6690.71
91_F131_L0.6680.71
46_E56_L0.6630.71
45_S51_S0.6590.70
273_T276_P0.6570.70
156_G202_R0.6560.70
239_Y279_L0.6550.70
99_E102_E0.6550.70
175_E178_I0.6540.70
191_P277_E0.6500.69
241_L271_L0.6500.69
28_S31_E0.6490.69
37_K47_E0.6460.69
13_H115_Y0.6450.69
30_E38_K0.6410.68
203_T221_R0.6400.68
145_N149_N0.6330.67
63_E66_K0.6300.67
137_R227_R0.6290.67
215_H218_N0.6280.67
47_E52_A0.6250.66
146_W161_D0.6250.66
57_N75_I0.6240.66
81_E92_K0.6240.66
252_E257_P0.6220.66
33_K89_E0.6220.66
198_S212_I0.6200.66
28_S33_K0.6160.65
254_T258_N0.6150.65
61_F70_G0.6150.65
67_K70_G0.6140.65
29_I34_K0.6130.65
98_P102_E0.6120.65
194_F226_V0.6070.64
137_R235_G0.6070.64
47_E50_E0.6060.64
189_I193_L0.6060.64
170_V196_W0.6040.64
194_F197_Q0.6040.64
46_E49_I0.6000.63
139_D142_R0.6000.63
34_K43_E0.5980.63
67_K74_L0.5980.63
252_E266_E0.5970.63
146_W208_E0.5960.63
234_E282_Y0.5960.63
51_S55_V0.5960.63
16_L39_K0.5950.62
30_E83_G0.5910.62
76_E89_E0.5900.62
20_L39_K0.5890.62
186_D189_I0.5880.62
178_I185_E0.5840.61
70_G74_L0.5840.61
37_K42_Y0.5830.61
154_N186_D0.5830.61
156_G161_D0.5820.61
156_G186_D0.5820.61
128_P155_I0.5820.61
66_K74_L0.5810.61
35_E89_E0.5800.61
255_T260_N0.5800.61
165_T221_R0.5780.60
62_T66_K0.5760.60
146_W167_P0.5740.60
254_T257_P0.5730.60
28_S35_E0.5720.59
26_S96_K0.5720.59
11_R51_S0.5720.59
254_T259_T0.5710.59
146_W165_T0.5700.59
20_L25_G0.5700.59
16_L35_E0.5670.59
57_N60_F0.5660.59
158_Y208_E0.5640.58
74_L77_Y0.5630.58
188_F271_L0.5630.58
26_S35_E0.5620.58
151_V156_G0.5600.58
150_E155_I0.5590.58
32_L36_L0.5590.58
21_L26_S0.5580.58
208_E265_V0.5580.58
65_E68_K0.5570.57
136_S236_K0.5570.57
230_K235_G0.5560.57
7_L269_W0.5560.57
24_N27_I0.5560.57
55_V58_L0.5560.57
132_Y241_L0.5530.57
197_Q267_I0.5520.57
134_K237_D0.5500.57
152_S199_K0.5490.56
56_L60_F0.5480.56
197_Q264_P0.5460.56
137_R160_Y0.5450.56
59_E66_K0.5440.56
161_D165_T0.5410.55
29_I50_E0.5390.55
195_H281_D0.5380.55
172_Y279_L0.5370.55
141_C199_K0.5370.55
63_E67_K0.5360.55
220_I241_L0.5350.54
227_R264_P0.5350.54
180_E258_N0.5350.54
83_G273_T0.5350.54
160_Y208_E0.5340.54
208_E212_I0.5330.54
132_Y271_L0.5330.54
92_K122_E0.5320.54
66_K71_D0.5310.54
146_W158_Y0.5310.54
119_Y127_T0.5290.54
137_R154_N0.5280.54
247_V283_L0.5280.54
12_P26_S0.5270.53
252_E255_T0.5270.53
83_G86_S0.5250.53
191_P280_Y0.5210.53
252_E258_N0.5210.53
150_E161_D0.5200.52
21_L28_S0.5190.52
187_Y266_E0.5190.52
26_S205_D0.5170.52
50_E54_N0.5160.52
197_Q269_W0.5140.52
61_F65_E0.5140.52
140_V144_L0.5130.51
118_Y130_K0.5110.51
141_C167_P0.5100.51
15_L107_L0.5090.51
229_N237_D0.5090.51
175_E180_E0.5070.51
16_L26_S0.5070.51
155_I199_K0.5070.51
149_N153_S0.5070.51
95_L134_K0.5060.51
143_L146_W0.5050.50
56_L61_F0.5050.50
10_K237_D0.5040.50
23_E105_L0.5040.50
51_S58_L0.5030.50
24_N30_E0.5030.50
160_Y246_P0.5020.50
16_L112_L0.5020.50
151_V155_I0.5000.50
197_Q254_T0.5000.50
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1bjaA 1 0.3063 21.5 0.962 Contact Map
2mh2A 1 0.2218 16.8 0.964 Contact Map
2k4jA 1 0.2817 16.8 0.964 Contact Map
1gxqA 1 0.2852 15.3 0.964 Contact Map
3rjpA 1 0.2817 14.2 0.965 Contact Map
1opcA 1 0.2852 13.7 0.965 Contact Map
2m87A 1 0.2817 11.6 0.966 Contact Map
1hsjA 2 0.3275 11.2 0.966 Contact Map
4nhjA 2 0.2852 10.5 0.967 Contact Map
2hwvA 1 0.2887 9.9 0.967 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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