GREMLIN Database
DUF3422 - Protein of unknown function (DUF3422)
PFAM: PF11902 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 419 (416)
Sequences: 2569 (1800)
Seq/√Len: 88.3
META: 0.722

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
370_S405_V5.7451.00
205_G267_T5.1161.00
383_G398_A4.9001.00
125_V185_A3.6291.00
222_E245_E3.6071.00
379_Y382_K3.3871.00
278_I296_M3.2241.00
152_V188_L3.0281.00
377_V400_A2.9361.00
28_S122_H2.8911.00
378_G397_T2.8841.00
382_K394_E2.8811.00
271_Y300_L2.7781.00
374_V404_V2.7741.00
378_G382_K2.7641.00
377_V404_V2.7631.00
159_V192_L2.7271.00
42_D83_S2.6551.00
403_V407_L2.6081.00
52_R108_D2.5771.00
123_L193_L2.5211.00
367_A373_A2.5161.00
377_V381_A2.4581.00
267_T304_M2.4571.00
7_Y11_N2.4031.00
159_V292_L2.3951.00
32_F113_L2.3571.00
229_R245_E2.3181.00
120_A170_F2.3031.00
43_R47_A2.2291.00
87_F105_V2.2251.00
282_R285_R2.2231.00
69_L72_F2.2201.00
315_E319_E2.2191.00
342_A345_A2.1791.00
156_A179_G2.1441.00
385_K394_E2.1391.00
55_V65_F2.1181.00
222_E252_E2.1141.00
406_L410_L2.1091.00
382_K385_K2.1081.00
161_T173_F2.1041.00
412_V415_I2.0981.00
381_A396_A2.0661.00
121_V173_F2.0631.00
29_H86_T2.0591.00
40_E44_A2.0531.00
218_R252_E1.9991.00
151_R155_G1.9611.00
26_R91_G1.9591.00
2_D117_L1.9561.00
5_L8_A1.9461.00
240_E338_A1.9241.00
215_R252_E1.9101.00
21_L25_A1.9021.00
329_R333_D1.8931.00
68_D73_R1.8811.00
212_L260_T1.8721.00
384_L389_L1.8471.00
271_Y304_M1.8281.00
151_R284_E1.8201.00
2_D7_Y1.8101.00
126_L176_F1.7861.00
215_R218_R1.7851.00
149_G289_L1.7671.00
395_L399_L1.7641.00
106_P109_W1.7631.00
410_L413_R1.7611.00
23_A185_A1.7521.00
157_A180_L1.7351.00
254_E323_R1.7301.00
360_T364_L1.7141.00
271_Y301_A1.6881.00
382_K393_P1.6721.00
152_V159_V1.6691.00
414_R417_R1.6511.00
48_A112_A1.6451.00
268_R308_E1.6301.00
291_T294_E1.6291.00
4_P116_Q1.6231.00
253_L314_L1.5831.00
148_V163_F1.5821.00
82_F201_M1.5791.00
180_L188_L1.5731.00
343_L346_S1.5641.00
202_A300_L1.5601.00
68_D72_F1.5581.00
123_L189_V1.5531.00
353_L356_R1.5521.00
51_D57_P1.5351.00
35_D116_Q1.5171.00
45_H113_L1.5151.00
380_L401_V1.5141.00
271_Y275_Q1.5081.00
50_C65_F1.5081.00
209_A264_F1.5021.00
216_L310_V1.5001.00
312_R316_A1.4961.00
208_V211_E1.4851.00
35_D38_D1.4700.99
373_A404_V1.4670.99
27_V189_V1.4630.99
175_V188_L1.4480.99
375_G379_Y1.4430.99
153_A188_L1.4240.99
105_V108_D1.4100.99
386_A394_E1.4060.99
392_S395_L1.3980.99
400_A404_V1.3980.99
69_L87_F1.3960.99
348_N352_R1.3780.99
142_F162_D1.3650.99
212_L314_L1.3560.99
253_L321_V1.3500.99
25_A88_F1.3490.99
375_G382_K1.3440.99
336_L340_N1.3300.99
48_A52_R1.3230.99
42_D85_Y1.3160.99
346_S351_A1.3110.99
133_P137_A1.3090.99
175_V192_L1.3070.99
268_R311_E1.3040.99
118_L200_M1.3040.99
347_M350_R1.3030.99
50_C58_P1.2900.99
412_V416_R1.2900.99
119_V196_E1.2840.99
58_P65_F1.2810.99
151_R158_V1.2800.99
63_T210_R1.2580.98
381_A400_A1.2530.98
228_A259_A1.2490.98
177_D185_A1.2470.98
7_Y165_I1.2310.98
29_H197_T1.2250.98
29_H84_T1.2200.98
273_L300_L1.2190.98
163_F333_D1.2190.98
220_E313_R1.2180.98
122_H168_D1.2160.98
411_G415_I1.1990.98
108_D111_A1.1960.98
19_P66_S1.1930.98
375_G378_G1.1840.98
345_A348_N1.1760.97
166_D171_S1.1710.97
265_S309_A1.1550.97
211_E259_A1.1530.97
227_T324_A1.1520.97
28_S101_A1.1510.97
395_L398_A1.1430.97
13_L18_F1.1420.97
19_P73_R1.1380.97
408_V412_V1.1220.97
28_S87_F1.1180.97
100_P122_H1.1070.96
126_L137_A1.1060.96
381_A393_P1.1020.96
149_G160_A1.0990.96
391_L396_A1.0960.96
177_D180_L1.0930.96
34_A38_D1.0890.96
276_Q297_E1.0860.96
208_V307_C1.0790.96
46_L85_Y1.0740.96
276_Q305_R1.0740.96
27_V88_F1.0740.96
238_D241_A1.0720.96
29_H336_L1.0700.96
262_F340_N1.0700.96
402_P406_L1.0690.96
208_V260_T1.0680.95
374_V401_V1.0670.95
122_H170_F1.0560.95
196_E296_M1.0550.95
142_F174_L1.0490.95
125_V189_V1.0440.95
18_F84_T1.0430.95
370_S404_V1.0430.95
150_S293_G1.0420.95
207_P307_C1.0380.95
45_H114_P1.0380.95
275_Q301_A1.0300.94
409_W413_R1.0280.94
121_V193_L1.0260.94
48_A51_D1.0200.94
231_A251_A1.0180.94
369_I375_G1.0170.94
157_A188_L1.0120.94
222_E248_A1.0090.94
360_T363_G1.0040.94
385_K388_G1.0020.94
144_G147_L1.0010.94
262_F265_S0.9990.94
121_V171_S0.9960.93
29_H329_R0.9950.93
384_L387_A0.9910.93
184_R294_E0.9820.93
117_L120_A0.9750.93
239_E331_R0.9720.93
282_R294_E0.9720.93
74_L87_F0.9680.92
213_S310_V0.9630.92
333_D337_E0.9610.92
370_S374_V0.9580.92
134_D138_L0.9570.92
22_E182_P0.9560.92
150_S291_T0.9530.92
254_E320_R0.9510.92
28_S102_L0.9490.92
219_I256_L0.9480.92
311_E314_L0.9470.92
85_Y113_L0.9460.92
150_S292_L0.9430.91
337_E375_G0.9390.91
386_A392_S0.9380.91
87_F101_A0.9370.91
250_A254_E0.9350.91
193_L197_T0.9350.91
336_L341_Q0.9340.91
158_V178_R0.9330.91
393_P397_T0.9310.91
240_E244_D0.9310.91
93_A124_A0.9300.91
32_F117_L0.9290.91
278_I293_G0.9270.91
150_S283_E0.9270.91
100_P103_D0.9260.91
380_L384_L0.9260.91
195_I278_I0.9240.91
271_Y308_E0.9230.91
152_V157_A0.9220.91
165_I171_S0.9210.90
374_V381_A0.9190.90
39_R42_D0.9170.90
194_E277_R0.9170.90
136_E139_A0.9160.90
374_V400_A0.9160.90
49_L106_P0.9130.90
21_L189_V0.9100.90
17_P197_T0.9080.90
195_I283_E0.9040.90
25_A90_P0.9040.90
382_K397_T0.8980.89
385_K396_A0.8970.89
38_D41_A0.8940.89
404_V415_I0.8940.89
10_A200_M0.8920.89
382_K390_P0.8910.89
10_A165_I0.8890.89
9_L200_M0.8880.89
219_I253_L0.8870.89
235_G238_D0.8850.89
95_R98_D0.8850.89
349_R353_L0.8810.88
43_R78_R0.8800.88
118_L201_M0.8790.88
83_S206_L0.8770.88
123_L173_F0.8760.88
187_R290_Q0.8680.88
189_V193_L0.8640.87
142_F147_L0.8630.87
225_E229_R0.8600.87
36_G39_R0.8600.87
44_A47_A0.8590.87
26_R122_H0.8580.87
46_L74_L0.8580.87
145_E166_D0.8560.87
350_R353_L0.8550.87
374_V397_T0.8550.87
254_E258_A0.8520.87
34_A42_D0.8510.87
101_A122_H0.8510.87
187_R320_R0.8500.87
212_L261_S0.8440.86
414_R418_R0.8440.86
44_A51_D0.8420.86
149_G286_I0.8390.86
248_A252_E0.8370.86
12_E15_A0.8360.86
66_S73_R0.8340.86
17_P283_E0.8330.85
224_A318_S0.8320.85
356_R359_Q0.8310.85
206_L317_L0.8250.85
319_E322_A0.8250.85
105_V109_W0.8250.85
272_A309_A0.8230.85
385_K393_P0.8220.85
99_P103_D0.8190.84
201_M212_L0.8180.84
43_R61_G0.8170.84
250_A321_V0.8140.84
28_S100_P0.8130.84
47_A57_P0.8130.84
41_A44_A0.8110.84
119_V197_T0.8100.84
190_Q298_R0.8080.84
72_F105_V0.8050.83
264_F310_V0.8030.83
382_K395_L0.7980.83
390_P396_A0.7970.83
171_S196_E0.7970.83
188_L281_L0.7960.83
392_S396_A0.7960.83
212_L256_L0.7950.83
363_G366_V0.7910.82
79_H82_F0.7910.82
32_F85_Y0.7910.82
237_E241_A0.7900.82
361_V364_L0.7880.82
64_H75_K0.7880.82
239_E335_E0.7870.82
413_R416_R0.7870.82
95_R140_R0.7840.82
364_L379_Y0.7840.82
269_A302_P0.7800.81
366_V409_W0.7800.81
154_D284_E0.7790.81
397_T401_V0.7790.81
134_D137_A0.7770.81
29_H119_V0.7770.81
161_T283_E0.7770.81
154_D287_E0.7710.81
279_A293_G0.7700.81
173_F192_L0.7690.81
45_H246_L0.7650.80
131_P134_D0.7640.80
325_S334_V0.7600.80
131_P175_V0.7540.79
286_I289_L0.7520.79
126_L133_P0.7520.79
202_A307_C0.7460.79
42_D78_R0.7450.78
154_D285_R0.7430.78
64_H409_W0.7410.78
385_K392_S0.7410.78
14_H17_P0.7400.78
256_L314_L0.7390.78
291_T301_A0.7360.78
205_G208_V0.7350.77
326_D329_R0.7340.77
23_A177_D0.7340.77
102_L170_F0.7330.77
255_R259_A0.7310.77
6_R12_E0.7280.77
345_A349_R0.7270.77
184_R300_L0.7260.77
27_V193_L0.7260.77
34_A39_R0.7250.77
78_R83_S0.7240.76
18_F31_A0.7240.76
405_V415_I0.7220.76
218_R222_E0.7210.76
160_A176_F0.7210.76
19_P75_K0.7200.76
130_D133_P0.7150.76
11_N84_T0.7130.75
89_R104_L0.7120.75
22_E90_P0.7110.75
394_E397_T0.7110.75
56_P65_F0.7100.75
122_H166_D0.7100.75
390_P399_L0.7100.75
202_A262_F0.7090.75
264_F283_E0.7040.74
163_F292_L0.7040.74
17_P75_K0.7040.74
380_L383_G0.7040.74
50_C55_V0.7030.74
49_L109_W0.6980.74
32_F45_H0.6960.74
155_G284_E0.6930.73
220_E326_D0.6920.73
360_T369_I0.6910.73
381_A385_K0.6910.73
407_L411_G0.6900.73
76_W83_S0.6890.73
196_E283_E0.6880.73
267_T271_Y0.6880.73
141_W174_L0.6850.72
285_R351_A0.6850.72
359_Q411_G0.6820.72
331_R335_E0.6800.72
112_A381_A0.6790.72
250_A332_V0.6790.72
45_H48_A0.6780.72
151_R178_R0.6780.72
240_E341_Q0.6770.72
150_S154_D0.6750.71
84_T201_M0.6740.71
374_V378_G0.6740.71
5_L11_N0.6740.71
154_D282_R0.6730.71
26_R89_R0.6710.71
72_F122_H0.6700.71
399_L403_V0.6700.71
17_P31_A0.6700.71
278_I292_L0.6690.71
359_Q416_R0.6690.71
215_R256_L0.6690.71
125_V175_V0.6670.70
387_A399_L0.6660.70
28_S89_R0.6650.70
184_R290_Q0.6630.70
163_F196_E0.6620.70
150_S195_I0.6620.70
296_M300_L0.6620.70
279_A384_L0.6610.70
206_L264_F0.6580.69
126_L174_L0.6580.69
53_F106_P0.6580.69
206_L402_P0.6580.69
190_Q245_E0.6580.69
116_Q130_D0.6580.69
130_D134_D0.6570.69
219_I252_E0.6570.69
222_E225_E0.6540.69
396_A399_L0.6520.69
16_R269_A0.6520.69
166_D172_R0.6510.69
201_M281_L0.6510.69
273_L367_A0.6480.68
37_R41_A0.6440.68
150_S159_V0.6430.68
22_E25_A0.6430.68
365_S368_A0.6430.68
311_E315_E0.6430.68
47_A51_D0.6430.68
3_H33_L0.6400.67
403_V406_L0.6390.67
218_R225_E0.6390.67
261_S316_A0.6380.67
340_N350_R0.6370.67
42_D76_W0.6350.67
229_R237_E0.6340.67
348_N359_Q0.6340.67
175_V189_V0.6340.67
308_E312_R0.6330.66
163_F205_G0.6330.66
45_H109_W0.6320.66
338_A341_Q0.6310.66
383_G388_G0.6300.66
400_A408_V0.6300.66
130_D417_R0.6300.66
220_E262_F0.6300.66
87_F203_L0.6270.66
277_R327_L0.6220.65
157_A175_V0.6210.65
109_W187_R0.6200.65
61_G132_A0.6190.65
251_A339_Q0.6180.65
153_A220_E0.6180.65
87_F104_L0.6150.64
29_H75_K0.6140.64
11_N45_H0.6140.64
64_H162_D0.6110.64
362_E365_S0.6100.64
67_A71_G0.6090.63
260_T314_L0.6080.63
230_M315_E0.6080.63
363_G370_S0.6050.63
41_A45_H0.6050.63
163_F340_N0.6040.63
45_H112_A0.6040.63
39_R262_F0.6020.63
171_S200_M0.6010.62
148_V311_E0.5990.62
406_L409_W0.5970.62
385_K398_A0.5960.62
40_E43_R0.5960.62
209_A310_V0.5960.62
251_A314_L0.5960.62
142_F145_E0.5950.62
217_A260_T0.5930.61
152_V285_R0.5930.61
180_L185_A0.5920.61
34_A83_S0.5920.61
148_V296_M0.5920.61
53_F108_D0.5920.61
254_E350_R0.5910.61
223_L374_V0.5900.61
148_V171_S0.5900.61
157_A181_G0.5900.61
11_N52_R0.5900.61
234_A316_A0.5890.61
64_H67_A0.5880.61
99_P400_A0.5860.60
219_I222_E0.5860.60
66_S86_T0.5850.60
365_S372_Y0.5850.60
359_Q418_R0.5830.60
184_R197_T0.5830.60
12_E16_R0.5830.60
288_G294_E0.5820.60
114_P333_D0.5820.60
209_A307_C0.5810.60
175_V406_L0.5790.60
389_L396_A0.5780.59
187_R282_R0.5770.59
394_E398_A0.5770.59
212_L267_T0.5770.59
216_L260_T0.5750.59
276_Q280_E0.5750.59
246_L359_Q0.5740.59
161_T271_Y0.5740.59
404_V412_V0.5730.59
252_E265_S0.5730.59
28_S170_F0.5730.59
406_L418_R0.5720.59
140_R179_G0.5720.59
57_P60_P0.5700.58
109_W166_D0.5690.58
339_Q351_A0.5690.58
407_L410_L0.5660.58
268_R312_R0.5660.58
127_P177_D0.5650.58
261_S320_R0.5640.58
25_A125_V0.5640.58
203_L306_T0.5610.57
227_T255_R0.5600.57
387_A395_L0.5590.57
17_P196_E0.5590.57
218_R245_E0.5580.57
285_R294_E0.5580.57
358_Q362_E0.5570.57
158_V332_V0.5560.56
16_R302_P0.5560.56
162_D333_D0.5560.56
361_V365_S0.5560.56
203_L303_A0.5550.56
368_A374_V0.5540.56
97_F172_R0.5530.56
377_V396_A0.5530.56
34_A37_R0.5510.56
402_P405_V0.5510.56
182_P409_W0.5500.56
119_V165_I0.5500.56
116_Q175_V0.5490.56
265_S311_E0.5490.56
402_P407_L0.5480.55
50_C337_E0.5470.55
18_F75_K0.5460.55
82_F194_E0.5460.55
243_L330_T0.5450.55
153_A280_E0.5450.55
374_V405_V0.5450.55
70_G97_F0.5450.55
359_Q413_R0.5440.55
410_L414_R0.5440.55
62_A337_E0.5430.55
320_R327_L0.5430.55
227_T318_S0.5430.55
240_E336_L0.5400.54
214_P387_A0.5400.54
18_F305_R0.5390.54
187_R216_L0.5390.54
20_P183_R0.5390.54
58_P62_A0.5390.54
121_V334_V0.5390.54
314_L325_S0.5390.54
410_L418_R0.5380.54
53_F70_G0.5380.54
50_C57_P0.5360.54
13_L296_M0.5360.54
109_W320_R0.5340.54
361_V375_G0.5340.54
321_V351_A0.5330.53
324_A340_N0.5320.53
393_P396_A0.5320.53
80_T262_F0.5320.53
38_D116_Q0.5300.53
381_A397_T0.5280.53
216_L256_L0.5250.52
395_L414_R0.5250.52
109_W113_L0.5240.52
32_F292_L0.5220.52
84_T212_L0.5220.52
295_F350_R0.5220.52
75_K274_V0.5200.52
95_R100_P0.5200.52
44_A48_A0.5180.51
29_H188_L0.5180.51
380_L403_V0.5170.51
133_P138_L0.5170.51
250_A329_R0.5170.51
376_L401_V0.5160.51
83_S124_A0.5160.51
39_R268_R0.5160.51
195_I322_A0.5160.51
102_L122_H0.5150.51
43_R84_T0.5150.51
222_E229_R0.5140.51
55_V69_L0.5130.51
82_F299_R0.5130.51
177_D184_R0.5130.51
94_A130_D0.5120.51
82_F302_P0.5110.50
283_E341_Q0.5110.50
135_A255_R0.5110.50
119_V171_S0.5080.50
156_A181_G0.5080.50
164_R378_G0.5070.50
125_V395_L0.5070.50
387_A391_L0.5070.50
70_G366_V0.5060.50
353_L360_T0.5060.50
326_D333_D0.5060.50
152_V195_I0.5060.50
239_E247_T0.5050.50
10_A171_S0.5050.50
118_L204_L0.5040.49
250_A312_R0.5030.49
99_P167_A0.5030.49
107_A111_A0.5020.49
219_I249_L0.5020.49
40_E61_G0.5020.49
367_A401_V0.5020.49
378_G385_K0.5020.49
360_T415_I0.5020.49
73_R90_P0.5020.49
47_A58_P0.5010.49
314_L336_L0.5000.49
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4ev6A 3 0.6993 22.7 0.978 Contact Map
2wpvA 1 0.2339 13.4 0.98 Contact Map
1rh5C 1 0.0764 7.7 0.982 Contact Map
4i0uA 4 0.7375 7.1 0.983 Contact Map
2l9uA 2 0.0859 7 0.983 Contact Map
2rmfA 1 0.0788 6.1 0.983 Contact Map
3htkB 1 0.1623 6 0.983 Contact Map
3p9yA 1 0.1933 5.6 0.984 Contact Map
2lzpA 1 0.0668 5.4 0.984 Contact Map
4p02A 1 0.1217 4.8 0.984 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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