GREMLIN Database
DUF3386 - Protein of unknown function (DUF3386)
PFAM: PF11866 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 211 (208)
Sequences: 969 (576)
Seq/√Len: 39.9
META: 0.811

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
29_D207_N6.9861.00
120_R140_S4.5061.00
123_M136_N4.2011.00
27_T44_T3.3581.00
177_R180_D3.1501.00
28_A43_V3.0891.00
11_A119_N3.0821.00
145_E177_R3.0541.00
27_T209_K2.9741.00
44_T52_E2.5921.00
158_D161_T2.4691.00
29_D42_K2.4031.00
89_T106_G2.3161.00
99_G102_E2.2911.00
112_S133_F2.0970.99
74_I152_Y2.0970.99
9_F13_Y2.0770.99
62_V66_A2.0680.99
96_D115_R2.0430.99
174_E186_S2.0050.99
51_V70_F2.0030.99
173_D187_R2.0010.99
51_V73_A1.8950.98
157_W164_L1.7890.98
113_K125_N1.7870.98
177_R182_W1.7450.97
97_E100_G1.7440.97
98_S101_V1.7420.97
3_P7_E1.6990.97
103_I116_V1.6950.97
186_S202_E1.6720.96
108_K133_F1.6130.96
64_W201_Q1.6050.95
83_E87_K1.5700.95
110_M133_F1.5350.94
138_E151_K1.5070.94
4_N7_E1.5030.94
73_A77_V1.5010.94
130_G158_D1.4840.93
198_E201_Q1.4810.93
7_E10_R1.4770.93
112_S154_S1.4630.93
20_D181_Y1.4540.92
178_V183_L1.4340.92
19_W107_G1.4280.92
139_E150_K1.4240.92
20_D24_P1.4230.92
100_G103_I1.4020.91
68_Q171_F1.3990.91
145_E180_D1.3920.91
46_G50_K1.3860.90
32_F39_H1.3830.90
93_G102_E1.3830.90
59_E62_V1.3760.90
56_I65_I1.3720.90
158_D165_K1.3540.89
85_H90_F1.3340.89
89_T92_L1.3240.88
24_P181_Y1.3220.88
135_I154_S1.3150.88
13_Y18_T1.3110.88
45_V73_A1.3090.88
186_S204_T1.3050.88
17_Y182_W1.3040.87
172_E190_K1.3020.87
183_L208_L1.2930.87
81_F86_G1.2690.86
125_N134_T1.2560.85
60_K63_K1.2540.85
71_E154_S1.2420.85
127_K132_I1.2350.84
10_R82_E1.2120.83
108_K112_S1.1930.82
10_R14_E1.1930.82
139_E151_K1.1900.82
176_V185_N1.1810.82
7_E11_A1.1750.81
85_H88_N1.1650.81
35_N199_E1.1570.80
117_K122_T1.1550.80
180_D185_N1.1460.80
26_Y205_F1.1400.79
37_K200_T1.1380.79
123_M155_Q1.1300.79
68_Q173_D1.1250.78
83_E183_L1.1120.78
199_E206_S1.1060.77
76_R124_V1.1030.77
37_K60_K1.0970.77
161_T165_K1.0950.76
33_N36_G1.0820.76
75_H135_I1.0790.75
80_S83_E1.0780.75
80_S127_K1.0760.75
124_V149_S1.0700.75
49_L175_F1.0640.74
33_N204_T1.0610.74
16_R19_W1.0550.74
80_S89_T1.0420.73
5_A28_A1.0320.72
18_T85_H1.0220.71
93_G96_D1.0180.71
119_N147_Y1.0070.70
13_Y16_R0.9960.69
112_S123_M0.9940.69
64_W139_E0.9680.67
87_K90_F0.9670.67
22_D181_Y0.9560.66
108_K154_S0.9540.66
125_N148_L0.9440.65
11_A14_E0.9430.65
47_A174_E0.9410.65
94_D102_E0.9320.64
40_K58_D0.9290.64
126_R135_I0.9270.64
133_F154_S0.9230.63
10_R145_E0.9170.63
195_N206_S0.9120.62
81_F85_H0.9050.62
5_A30_C0.9040.62
95_T103_I0.9030.62
15_N147_Y0.9010.61
27_T41_G0.8960.61
90_F93_G0.8950.61
93_G104_L0.8910.61
31_T206_S0.8850.60
107_G111_G0.8840.60
116_V121_I0.8830.60
88_N92_L0.8790.60
6_R19_W0.8780.59
112_S129_H0.8740.59
29_D40_K0.8730.59
91_T106_G0.8710.59
5_A8_L0.8660.58
68_Q107_G0.8540.57
4_N139_E0.8490.57
195_N204_T0.8480.57
2_D39_H0.8410.56
55_G64_W0.8310.55
90_F99_G0.8270.55
13_Y81_F0.8260.55
142_D145_E0.8230.55
81_F173_D0.8210.54
74_I77_V0.8200.54
93_G103_I0.8170.54
69_L137_V0.8150.54
13_Y17_Y0.8140.54
53_V56_I0.8090.53
185_N204_T0.8080.53
41_G162_G0.8050.53
71_E133_F0.7980.52
16_R124_V0.7950.52
79_R129_H0.7940.52
74_I133_F0.7890.51
158_D163_E0.7850.51
177_R208_L0.7840.51
9_F45_V0.7820.51
183_L210_L0.7800.51
108_K132_I0.7780.50
141_L150_K0.7770.50
10_R22_D0.7740.50
110_M149_S0.7730.50
67_S134_T0.7660.49
32_F57_E0.7660.49
175_F182_W0.7630.49
76_R133_F0.7610.49
72_V149_S0.7580.49
9_F16_R0.7530.48
56_I63_K0.7520.48
106_G116_V0.7510.48
88_N109_N0.7490.48
20_D179_G0.7490.48
18_T81_F0.7470.48
92_L101_V0.7460.47
2_D116_V0.7440.47
83_E86_G0.7440.47
105_V109_N0.7410.47
66_A70_F0.7400.47
205_F208_L0.7240.45
9_F12_A0.7220.45
116_V179_G0.7220.45
21_N38_I0.7140.45
115_R136_N0.7130.44
3_P116_V0.7130.44
46_G83_E0.7120.44
81_F88_N0.7080.44
63_K66_A0.7010.43
68_Q128_I0.6970.43
122_T140_S0.6910.42
9_F19_W0.6900.42
197_E206_S0.6880.42
52_E161_T0.6860.42
99_G103_I0.6860.42
92_L99_G0.6810.42
193_E199_E0.6800.42
25_G28_A0.6800.42
81_F187_R0.6790.41
135_I152_Y0.6750.41
143_T182_W0.6750.41
77_V107_G0.6730.41
148_L162_G0.6730.41
8_L92_L0.6720.41
90_F102_E0.6670.40
178_V181_Y0.6660.40
68_Q74_I0.6610.40
62_V195_N0.6600.40
195_N198_E0.6510.39
107_G156_Y0.6490.39
71_E148_L0.6480.39
196_G202_E0.6480.39
65_I203_F0.6480.39
59_E186_S0.6390.38
196_G209_K0.6370.38
166_S169_E0.6340.37
84_S87_K0.6310.37
6_R82_E0.6260.37
23_F28_A0.6250.37
54_Q60_K0.6230.37
26_Y68_Q0.6220.36
30_C65_I0.6160.36
199_E202_E0.6140.36
6_R10_R0.6130.36
10_R37_K0.6120.36
85_H107_G0.6120.36
127_K131_T0.6110.36
96_D100_G0.6100.35
38_I160_K0.6100.35
123_M132_I0.6090.35
148_L178_V0.6090.35
42_K207_N0.6070.35
92_L98_S0.6070.35
19_W152_Y0.6040.35
33_N202_E0.5970.34
197_E202_E0.5960.34
53_V143_T0.5910.34
118_D138_E0.5900.34
75_H124_V0.5900.34
132_I157_W0.5880.34
157_W165_K0.5880.34
21_N52_E0.5870.34
57_E82_E0.5830.33
32_F138_E0.5790.33
42_K55_G0.5780.33
104_L110_M0.5780.33
76_R126_R0.5770.33
74_I129_H0.5770.33
6_R81_F0.5770.33
197_E201_Q0.5760.33
4_N15_N0.5740.32
120_R147_Y0.5740.32
112_S132_I0.5730.32
92_L95_T0.5720.32
14_E143_T0.5670.32
6_R13_Y0.5670.32
190_K198_E0.5640.32
42_K179_G0.5640.32
21_N83_E0.5640.32
9_F114_Y0.5630.32
119_N149_S0.5630.32
67_S202_E0.5630.32
36_G160_K0.5620.32
188_T192_L0.5620.32
19_W81_F0.5580.31
18_T48_D0.5560.31
9_F107_G0.5560.31
114_Y121_I0.5530.31
88_N111_G0.5510.31
16_R20_D0.5500.31
44_T160_K0.5500.31
178_V185_N0.5440.30
10_R171_F0.5440.30
42_K209_K0.5420.30
32_F139_E0.5410.30
63_K67_S0.5400.30
40_K119_N0.5400.30
188_T204_T0.5390.30
104_L118_D0.5370.30
119_N122_T0.5360.30
80_S195_N0.5360.30
10_R59_E0.5350.29
177_R202_E0.5340.29
57_E196_G0.5340.29
178_V189_I0.5330.29
9_F90_F0.5290.29
43_V64_W0.5270.29
128_I154_S0.5240.29
32_F41_G0.5240.29
127_K156_Y0.5210.28
54_Q195_N0.5200.28
65_I108_K0.5190.28
117_K120_R0.5180.28
8_L12_A0.5170.28
138_E149_S0.5170.28
34_Q61_V0.5150.28
13_Y85_H0.5150.28
130_G144_G0.5130.28
203_F210_L0.5130.28
71_E184_L0.5130.28
151_K172_E0.5120.28
134_T162_G0.5120.28
16_R147_Y0.5110.28
193_E200_T0.5110.28
75_H112_S0.5090.27
90_F152_Y0.5080.27
74_I154_S0.5080.27
53_V73_A0.5080.27
71_E112_S0.5070.27
188_T193_E0.5070.27
128_I133_F0.5050.27
115_R137_V0.5020.27
21_N31_T0.5000.27
156_Y169_E0.5000.27
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3buuA 1 0.8673 51.9 0.945 Contact Map
4mxtA 2 0.7346 26.8 0.954 Contact Map
1v5iB 1 0.2559 15.2 0.959 Contact Map
3m4wA 2 0.7393 13.5 0.959 Contact Map
2pqrC 1 0.1706 10.1 0.962 Contact Map
2hyxA 2 0.346 8.9 0.963 Contact Map
4ipaA 1 0.3318 7.5 0.964 Contact Map
4nmxA 1 0.2559 6.8 0.965 Contact Map
4z48A 1 0.9005 6.8 0.965 Contact Map
3bk5A 1 0.8768 6.6 0.965 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.007 seconds.