GREMLIN Database
DUF3332 - Domain of unknown function (DUF3332)
PFAM: PF11810 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 169 (164)
Sequences: 1004 (777)
Seq/√Len: 60.7
META: 0.798

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
60_A65_L4.6111.00
18_I32_G4.3671.00
42_E46_L4.2731.00
34_V44_V3.8041.00
42_E45_F3.4991.00
24_T28_Y2.8721.00
59_L63_L2.5621.00
46_L50_I2.3811.00
24_T58_A2.3631.00
60_A64_V2.3551.00
65_L77_I2.2791.00
99_T108_T2.2201.00
122_D125_E2.1711.00
31_N54_Y2.1311.00
49_W55_G2.1231.00
5_A8_L2.1191.00
42_E67_S2.1171.00
47_A50_I2.0631.00
31_N52_P2.0281.00
18_I28_Y1.9991.00
106_T118_D1.9141.00
21_F45_F1.9141.00
9_A13_L1.8901.00
49_W62_A1.8571.00
112_G115_K1.8541.00
52_P55_G1.8241.00
46_L67_S1.8161.00
58_A62_A1.7160.99
28_Y32_G1.6880.99
32_G41_N1.6880.99
45_F49_W1.6860.99
100_T105_Y1.6690.99
10_G14_L1.6510.99
125_E138_K1.6250.99
149_K159_T1.5730.99
81_V102_K1.5690.99
27_V31_N1.5560.99
129_S138_K1.5100.98
131_E136_S1.5060.98
96_Y128_W1.4700.98
31_N34_V1.4210.97
25_N28_Y1.4080.97
18_I30_W1.3980.97
127_S138_K1.3930.97
24_T57_A1.3840.97
24_T49_W1.3630.97
56_V60_A1.3420.96
20_S25_N1.3400.96
27_V57_A1.3240.96
47_A51_I1.3180.96
98_V105_Y1.2900.95
40_V44_V1.2490.94
68_I72_T1.2420.94
18_I42_E1.2120.94
27_V53_V1.1940.93
35_T44_V1.1570.92
11_S15_S1.1360.91
143_N147_T1.1280.91
8_L11_S1.1110.90
23_L77_I1.0970.90
31_N51_I1.0960.90
84_T99_T1.0850.89
35_T41_N1.0760.89
141_T151_Y1.0760.89
22_A75_N1.0730.89
40_V43_L1.0680.88
20_S69_E1.0640.88
125_E129_S1.0540.88
22_A77_I1.0430.87
45_F62_A1.0380.87
159_T162_L1.0350.87
5_A9_A1.0250.86
149_K157_V1.0200.86
12_L15_S1.0180.86
160_V163_D1.0160.86
27_V54_Y1.0150.86
146_G151_Y1.0080.85
38_K41_N1.0080.85
126_N131_E0.9880.84
64_V68_I0.9850.84
23_L57_A0.9800.84
144_D147_T0.9700.83
119_L130_V0.9680.83
49_W58_A0.9660.83
120_V131_E0.9610.82
74_S78_A0.9540.82
132_A135_E0.9490.82
127_S141_T0.9490.82
163_D166_G0.9370.81
18_I41_N0.9160.79
121_Y128_W0.9150.79
35_T40_V0.9130.79
63_L67_S0.9090.79
82_G88_N0.9080.79
52_P57_A0.8950.78
33_S74_S0.8880.77
162_L166_G0.8820.77
108_T116_V0.8810.77
130_V135_E0.8780.76
30_W41_N0.8710.76
103_N123_K0.8650.75
83_E87_V0.8600.75
107_I119_L0.8600.75
18_I27_V0.8580.75
138_K141_T0.8520.74
49_W52_P0.8410.73
31_N50_I0.8390.73
34_V41_N0.8370.73
27_V42_E0.8170.71
34_V37_N0.8080.70
29_K43_L0.8080.70
27_V60_A0.8070.70
50_I55_G0.8010.69
100_T103_N0.7940.69
25_N58_A0.7920.69
153_P159_T0.7870.68
158_M162_L0.7850.68
102_K123_K0.7830.68
31_N41_N0.7690.66
83_E103_N0.7660.66
90_E93_G0.7580.65
82_G103_N0.7580.65
130_V137_V0.7540.65
88_N94_R0.7490.64
18_I25_N0.7430.64
51_I54_Y0.7430.64
9_A12_L0.7350.63
87_V100_T0.7310.62
22_A26_K0.7290.62
48_F51_I0.7280.62
87_V90_E0.7270.62
152_L160_V0.7230.61
30_W45_F0.7170.61
150_V155_G0.7150.61
50_I54_Y0.7130.60
97_L104_G0.7110.60
151_Y157_V0.7080.60
101_T104_G0.7050.60
87_V98_V0.7040.59
109_K113_G0.7030.59
141_T145_D0.7030.59
152_L156_K0.6930.58
47_A55_G0.6900.58
138_K143_N0.6870.58
28_Y46_L0.6860.57
22_A78_A0.6850.57
65_L72_T0.6850.57
32_G42_E0.6840.57
138_K151_Y0.6810.57
79_M85_K0.6800.57
45_F51_I0.6780.57
150_V158_M0.6770.56
79_M83_E0.6760.56
47_A52_P0.6640.55
96_Y105_Y0.6630.55
150_V157_V0.6630.55
120_V136_S0.6600.55
140_F143_N0.6580.54
30_W33_S0.6530.54
97_L108_T0.6430.53
28_Y49_W0.6360.52
84_T87_V0.6350.52
140_F148_V0.6330.51
161_E164_E0.6320.51
60_A63_L0.6300.51
118_D131_E0.6280.51
26_K77_I0.6280.51
51_I55_G0.6260.51
43_L47_A0.6240.50
81_V85_K0.6240.50
150_V162_L0.6210.50
97_L110_E0.6210.50
37_N40_V0.6160.50
79_M101_T0.6110.49
38_K70_F0.6110.49
88_N95_L0.6060.48
7_L10_G0.6060.48
133_D136_S0.6040.48
46_L57_A0.6040.48
136_S153_P0.6030.48
89_G112_G0.6000.48
4_A8_L0.5970.47
20_S42_E0.5960.47
27_V109_K0.5950.47
22_A27_V0.5940.47
18_I21_F0.5940.47
58_A74_S0.5940.47
59_L90_E0.5930.47
141_T144_D0.5930.47
98_V107_I0.5870.46
123_K126_N0.5860.46
39_F42_E0.5830.46
100_T104_G0.5830.46
16_S41_N0.5820.46
59_L62_A0.5820.46
82_G100_T0.5770.45
109_K112_G0.5730.45
16_S30_W0.5720.45
21_F62_A0.5710.45
64_V85_K0.5690.44
102_K106_T0.5680.44
10_G13_L0.5680.44
28_Y63_L0.5650.44
21_F42_E0.5610.43
57_A65_L0.5610.43
133_D164_E0.5560.43
82_G87_V0.5540.43
161_E166_G0.5530.43
94_R97_L0.5500.42
21_F28_Y0.5490.42
31_N45_F0.5480.42
42_E54_Y0.5480.42
37_N63_L0.5440.42
96_Y127_S0.5410.41
59_L156_K0.5380.41
21_F32_G0.5360.41
32_G62_A0.5350.41
82_G121_Y0.5340.40
146_G162_L0.5340.40
23_L67_S0.5340.40
86_K94_R0.5330.40
18_I31_N0.5330.40
101_T112_G0.5320.40
47_A60_A0.5300.40
121_Y126_N0.5290.40
48_F55_G0.5250.39
8_L13_L0.5240.39
23_L26_K0.5230.39
49_W57_A0.5190.39
86_K92_D0.5180.39
21_F24_T0.5170.39
92_D107_I0.5150.38
138_K146_G0.5140.38
31_N44_V0.5130.38
43_L57_A0.5110.38
20_S28_Y0.5100.38
6_L12_L0.5100.38
55_G59_L0.5100.38
8_L15_S0.5090.38
36_G41_N0.5080.38
117_V130_V0.5060.37
27_V50_I0.5040.37
39_F67_S0.5030.37
78_A83_E0.5030.37
122_D129_S0.5010.37
6_L13_L0.5000.37
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1fftB 1 0.6982 18.7 0.943 Contact Map
4n4rB 1 0.7278 15.1 0.945 Contact Map
3u4yA 1 0.5266 9.6 0.95 Contact Map
1zvtA 1 0.4024 6.7 0.953 Contact Map
4r9fA 1 0.6272 5.4 0.955 Contact Map
4jwoA 1 0.6272 4.9 0.956 Contact Map
3l6vA 1 0.4793 4.5 0.957 Contact Map
3uorA 1 0.6095 4.3 0.957 Contact Map
3woaA 1 0.7574 4.3 0.957 Contact Map
2vozA 3 0.6923 4.2 0.957 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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