GREMLIN Database
DUF3328 - Domain of unknown function (DUF3328)
PFAM: PF11807 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 220 (211)
Sequences: 1126 (1014)
Seq/√Len: 69.8
META: 0.032

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
60_E209_D5.1281.00
132_H135_H3.9601.00
99_P102_E3.5691.00
135_H165_H3.4691.00
165_H168_H3.3131.00
171_D174_R3.2111.00
138_N141_R3.1241.00
171_D175_Q3.1021.00
130_V181_A3.0741.00
137_L177_L3.0161.00
209_D212_A2.9381.00
135_H168_H2.6961.00
58_E212_A2.4921.00
84_D141_R2.4481.00
138_N142_K2.3511.00
131_F210_F2.2241.00
144_L170_L2.1821.00
132_H168_H1.9751.00
84_D148_Y1.9261.00
74_I86_A1.9151.00
17_L24_S1.9041.00
17_L21_L1.8391.00
96_I100_E1.7861.00
173_L177_L1.7621.00
77_G167_D1.7471.00
64_F205_H1.7421.00
131_F214_R1.6761.00
178_M210_F1.6701.00
38_S41_C1.6530.99
132_H165_H1.6280.99
92_D96_I1.6200.99
215_E219_E1.6180.99
144_L163_R1.6140.99
172_Y186_E1.5920.99
165_H169_C1.5890.99
197_P200_D1.5840.99
58_E209_D1.5520.99
80_S83_V1.5500.99
53_A56_A1.5460.99
174_R178_M1.5360.99
134_L174_R1.5140.99
210_F214_R1.5000.99
21_L24_S1.4630.99
183_V208_R1.4600.99
60_E64_F1.4590.99
84_D149_Y1.4560.99
98_I126_A1.4330.98
211_D215_E1.4230.98
143_A146_P1.3930.98
19_L23_L1.3920.98
132_H136_C1.3830.98
108_L200_D1.3750.98
28_L31_A1.3750.98
79_P83_V1.3630.98
183_V210_F1.3240.97
84_D138_N1.3090.97
19_L24_S1.2880.97
137_L170_L1.2750.97
45_K48_S1.2670.97
15_L19_L1.2580.96
16_L22_L1.2350.96
40_S43_C1.2270.96
129_E186_E1.2160.96
130_V210_F1.2150.96
83_V86_A1.2060.95
133_Q177_L1.1960.95
129_E133_Q1.1950.95
183_V213_I1.1940.95
22_L32_L1.1930.95
137_L141_R1.1840.95
18_L22_L1.1830.95
59_Y65_N1.1830.95
75_Y88_D1.1810.95
85_A89_D1.1800.95
190_W197_P1.1770.95
97_R123_G1.1730.95
20_L24_S1.1680.95
15_L18_L1.1570.94
36_S40_S1.1550.94
29_L38_S1.1510.94
81_P148_Y1.1500.94
100_E104_A1.1380.94
139_L166_L1.1190.93
25_L29_L1.1150.93
16_L19_L1.1060.93
118_D121_G1.1050.93
51_S183_V1.0970.93
140_L166_L1.0770.92
181_A208_R1.0730.92
180_H208_R1.0720.92
24_S28_L1.0670.92
52_P55_D1.0670.92
15_L25_L1.0640.91
138_N210_F1.0510.91
129_E172_Y1.0450.91
103_A110_P1.0230.90
74_I77_G1.0230.90
14_L19_L1.0160.89
49_S59_Y1.0120.89
20_L35_S1.0110.89
48_S54_L1.0100.89
104_A109_P1.0100.89
103_A108_L1.0040.89
133_Q172_Y1.0030.89
19_L22_L0.9940.88
140_L144_L0.9890.88
49_S52_P0.9780.88
130_V177_L0.9710.87
130_V183_V0.9570.86
22_L34_L0.9560.86
18_L25_L0.9540.86
128_L217_A0.9530.86
96_I128_L0.9410.85
24_S27_L0.9410.85
21_L39_S0.9390.85
77_G83_V0.9340.85
215_E218_E0.9260.85
103_A124_Y0.9140.84
146_P149_Y0.9140.84
131_F217_A0.9120.84
117_P120_D0.9050.83
144_L147_D0.9010.83
33_L36_S0.9000.83
32_L35_S0.9000.83
75_Y174_R0.8970.83
129_E176_S0.8970.83
176_S180_H0.8920.82
186_E201_F0.8910.82
181_A210_F0.8870.82
19_L26_L0.8840.82
27_L31_A0.8780.81
96_I126_A0.8730.81
140_L170_L0.8700.81
133_Q176_S0.8690.81
186_E202_N0.8690.81
20_L25_L0.8660.80
26_L30_L0.8660.80
184_T205_H0.8650.80
13_L34_L0.8620.80
98_I102_E0.8590.80
59_Y64_F0.8520.79
44_L48_S0.8500.79
83_V149_Y0.8440.79
116_I121_G0.8430.79
126_A185_L0.8400.78
29_L35_S0.8400.78
38_S42_D0.8370.78
181_A213_I0.8280.77
182_D217_A0.8270.77
46_S49_S0.8230.77
47_L52_P0.8220.77
114_V121_G0.8180.76
129_E175_Q0.8070.75
14_L21_L0.8040.75
119_E122_G0.8030.75
92_D100_E0.8010.75
15_L21_L0.7990.75
69_F75_Y0.7950.74
36_S39_S0.7950.74
26_L43_C0.7920.74
127_G186_E0.7890.74
145_Y167_D0.7860.73
103_A107_G0.7850.73
80_S84_D0.7840.73
89_D218_E0.7830.73
175_Q190_W0.7830.73
19_L25_L0.7820.73
140_L147_D0.7810.73
95_A99_P0.7790.73
75_Y86_A0.7790.73
128_L185_L0.7780.73
49_S64_F0.7780.73
17_L25_L0.7780.73
176_S181_A0.7740.72
145_Y149_Y0.7700.72
16_L37_S0.7680.72
211_D214_R0.7640.71
51_S55_D0.7600.71
140_L160_E0.7540.70
129_E184_T0.7530.70
26_L32_L0.7490.70
17_L34_L0.7470.70
103_A187_T0.7450.69
22_L25_L0.7420.69
143_A166_L0.7370.69
17_L20_L0.7340.68
114_V119_E0.7340.68
18_L40_S0.7330.68
79_P149_Y0.7280.68
115_K118_D0.7220.67
100_E124_Y0.7210.67
213_I217_A0.7150.66
98_I103_A0.7140.66
45_K51_S0.7100.66
8_R12_R0.7060.65
8_R22_L0.7020.65
20_L23_L0.7000.65
19_L42_D0.6990.65
178_M203_T0.6980.64
46_S50_Y0.6970.64
18_L24_S0.6950.64
49_S53_A0.6920.64
99_P123_G0.6920.64
14_L18_L0.6880.63
178_M218_E0.6880.63
8_R20_L0.6850.63
178_M183_V0.6850.63
129_E205_H0.6850.63
28_L41_C0.6830.63
9_R12_R0.6800.62
16_L35_S0.6770.62
39_S43_C0.6760.62
138_N148_Y0.6730.62
114_V117_P0.6710.61
10_W13_L0.6680.61
126_A187_T0.6680.61
172_Y185_L0.6650.61
98_I106_L0.6630.60
26_L39_S0.6610.60
33_L41_C0.6600.60
50_Y55_D0.6590.60
20_L36_S0.6540.59
61_T204_V0.6530.59
19_L30_L0.6530.59
50_Y53_A0.6520.59
23_L26_L0.6520.59
208_R213_I0.6520.59
15_L38_S0.6520.59
29_L34_L0.6500.59
179_C207_C0.6460.58
95_A98_I0.6430.58
54_L61_T0.6430.58
21_L37_S0.6430.58
167_D174_R0.6330.57
108_L177_L0.6310.57
29_L32_L0.6310.57
27_L39_S0.6300.57
157_G161_E0.6300.57
101_E104_A0.6290.56
128_L131_F0.6260.56
109_P126_A0.6250.56
129_E173_L0.6210.55
32_L39_S0.6190.55
59_Y216_W0.6180.55
131_F138_N0.6140.55
77_G80_S0.6130.55
21_L28_L0.6090.54
154_L157_G0.6080.54
139_L146_P0.6050.54
81_P85_A0.6020.53
138_N208_R0.6020.53
129_E203_T0.5980.53
15_L32_L0.5970.53
14_L17_L0.5960.52
26_L29_L0.5920.52
8_R11_R0.5880.52
51_S57_I0.5860.51
37_S40_S0.5860.51
14_L39_S0.5850.51
23_L219_E0.5840.51
116_I119_E0.5820.51
16_L30_L0.5810.51
107_G184_T0.5790.50
136_C169_C0.5780.50
23_L30_L0.5750.50
95_A201_F0.5730.50
94_G204_V0.5720.50
117_P121_G0.5660.49
153_G159_P0.5660.49
129_E201_F0.5660.49
13_L18_L0.5650.49
19_L34_L0.5620.48
24_S30_L0.5620.48
19_L33_L0.5600.48
54_L59_Y0.5540.47
51_S54_L0.5520.47
190_W196_G0.5520.47
137_L140_L0.5510.47
26_L37_S0.5510.47
75_Y167_D0.5490.47
133_Q201_F0.5470.47
127_G130_V0.5450.46
151_Y154_L0.5390.46
17_L42_D0.5380.45
39_S42_D0.5380.45
11_R20_L0.5370.45
129_E210_F0.5360.45
174_R205_H0.5340.45
185_L206_T0.5340.45
16_L23_L0.5330.45
214_R218_E0.5320.45
37_S41_C0.5310.45
13_L25_L0.5300.45
19_L32_L0.5300.45
12_R16_L0.5280.44
151_Y159_P0.5270.44
196_G200_D0.5270.44
10_W14_L0.5260.44
153_G158_P0.5240.44
26_L34_L0.5240.44
49_S55_D0.5240.44
169_C179_C0.5220.44
126_A190_W0.5170.43
54_L57_I0.5160.43
98_I107_G0.5090.42
48_S51_S0.5090.42
156_D185_L0.5090.42
148_Y187_T0.5080.42
31_A34_L0.5070.42
28_L32_L0.5070.42
171_D178_M0.5060.42
98_I185_L0.5060.42
68_S175_Q0.5050.42
14_L20_L0.5050.42
34_L40_S0.5030.41
176_S205_H0.5000.41
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4xtrG 1 0.1 31.9 0.935 Contact Map
3j2vC 4 0.4045 20.2 0.941 Contact Map
2xf7A 3 0.1909 15.9 0.944 Contact Map
3v6zE 1 0.4318 15.9 0.944 Contact Map
3kxsF 2 0.4045 14.9 0.945 Contact Map
3hd7A 1 0.1636 11.4 0.947 Contact Map
4um9B 2 0 5.9 0.954 Contact Map
2kngA 1 0.1136 5.8 0.954 Contact Map
2m8rA 1 0.1682 5.1 0.955 Contact Map
2kncA 1 0.2318 3.4 0.959 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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