GREMLIN Database
DUF3326 - Protein of unknown function (DUF3326)
PFAM: PF11805 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 337 (335)
Sequences: 747 (530)
Seq/√Len: 29.0
META: 0.74

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
82_L100_A6.6661.00
314_V323_V5.2881.00
6_V21_A5.2711.00
2_A32_C5.1681.00
294_R315_E4.8641.00
208_A237_V4.3981.00
73_Q259_D4.2661.00
122_G138_E4.0011.00
51_P54_A3.7081.00
4_L24_V3.5451.00
25_A35_L3.4751.00
64_R69_T3.4321.00
148_E201_F3.3741.00
2_A324_L3.1841.00
5_I62_L3.1781.00
31_V325_A2.9701.00
151_I203_I2.8421.00
25_A41_V2.7261.00
83_V197_I2.6861.00
61_A247_I2.6371.00
102_A226_A2.5950.99
71_G261_T2.5670.99
97_L101_D2.2810.99
318_L333_P2.2680.99
2_A269_V2.2400.98
118_D145_R2.2010.98
27_L321_A2.1750.98
140_P196_L2.1210.98
157_T163_A2.0900.97
305_E313_E2.0270.97
321_A325_A2.0000.97
294_R313_E1.9760.96
110_D332_S1.9680.96
124_E138_E1.9640.96
37_T54_A1.9570.96
81_G150_L1.9040.96
70_W247_I1.8880.95
289_P327_L1.8810.95
32_C35_L1.8720.95
64_R202_Q1.8560.95
147_A197_I1.8010.94
83_V146_A1.7930.94
58_E195_H1.7510.93
3_V265_V1.7470.93
28_L321_A1.7040.92
303_T306_A1.7010.92
53_N78_N1.6890.92
4_L25_A1.6810.92
28_L269_V1.6790.92
162_V233_F1.6780.92
82_L234_L1.6700.91
29_A35_L1.6630.91
157_T161_V1.6620.91
270_I290_V1.6610.91
112_T156_A1.6540.91
270_I280_V1.6280.90
34_R74_P1.6050.90
13_E297_K1.5710.89
107_L235_P1.5630.88
100_A111_I1.5470.88
148_E200_E1.5370.87
121_L142_S1.5140.87
273_G298_T1.5070.86
267_A324_L1.5000.86
80_I189_V1.4960.86
71_G248_V1.4880.86
14_I24_V1.4860.86
38_H41_V1.4710.85
170_D182_G1.4670.85
10_I274_A1.4610.85
128_S134_W1.4520.84
84_L96_H1.4370.84
278_L282_A1.4300.83
111_I156_A1.4300.83
332_S335_S1.3970.82
269_V321_A1.3910.81
174_L177_Y1.3770.81
90_P94_L1.3770.81
78_N246_Q1.3680.80
85_D232_T1.3610.80
65_F265_V1.3330.79
174_L178_R1.3250.78
268_L280_V1.3230.78
158_A206_A1.3120.77
99_A229_I1.2990.77
304_P313_E1.2920.76
156_A161_V1.2880.76
155_G161_V1.2800.76
197_I205_C1.2790.76
79_R112_T1.2600.74
156_A163_A1.2490.74
281_L291_I1.2480.74
23_P105_A1.2440.74
319_E322_G1.2440.74
4_L22_L1.2410.73
144_L201_F1.2400.73
49_W239_K1.2350.73
36_I159_I1.2350.73
18_A23_P1.2320.73
83_V147_A1.2310.73
283_A288_I1.2280.73
18_A21_A1.2230.72
328_R332_S1.2070.71
34_R55_L1.2060.71
212_E233_F1.1900.70
31_V321_A1.1850.70
179_Q219_V1.1830.70
272_A294_R1.1800.69
180_G224_A1.1790.69
155_G163_A1.1710.69
206_A237_V1.1710.69
18_A45_A1.1590.68
80_I263_D1.1550.68
128_S201_F1.1540.68
295_E318_L1.1470.67
174_L214_L1.1340.66
272_A292_A1.1310.66
232_T235_P1.1270.66
3_V283_A1.1260.66
300_L311_V1.1230.66
6_V163_A1.1220.65
62_L65_F1.1190.65
184_D230_S1.1110.65
157_T246_Q1.0990.64
197_I223_R1.0990.64
324_L328_R1.0880.63
92_L95_R1.0850.63
211_L232_T1.0830.63
101_D219_V1.0800.62
198_S203_I1.0780.62
22_L159_I1.0720.62
221_D289_P1.0710.62
50_M239_K1.0500.60
316_N319_E1.0480.60
225_A231_Y1.0460.60
78_N157_T1.0350.59
248_V259_D1.0310.59
133_S147_A1.0290.59
100_A222_P1.0210.58
322_G326_A1.0210.58
86_A116_V1.0200.58
44_A106_T1.0160.58
99_A236_C1.0130.57
198_S247_I1.0110.57
234_L237_V1.0090.57
120_P233_F1.0070.57
50_M54_A1.0050.57
292_A311_V0.9860.55
123_V193_I0.9840.55
87_G122_G0.9840.55
172_E176_N0.9820.55
208_A234_L0.9780.55
9_G16_G0.9780.55
9_G15_G0.9780.55
63_D150_L0.9780.55
322_G330_G0.9780.55
323_V332_S0.9750.55
304_P307_L0.9720.54
48_Y239_K0.9690.54
300_L303_T0.9690.54
174_L211_L0.9650.54
53_N298_T0.9650.54
98_N101_D0.9640.54
139_N142_S0.9530.53
318_L323_V0.9530.53
162_V232_T0.9420.52
314_V320_A0.9380.52
312_I327_L0.9350.51
148_E243_R0.9350.51
139_N225_A0.9340.51
38_H62_L0.9340.51
80_I110_D0.9310.51
28_L324_L0.9220.50
325_A333_P0.9210.50
60_Y198_S0.9180.50
105_A229_I0.9130.50
119_A139_N0.9120.50
13_E50_M0.9090.49
137_I189_V0.9060.49
213_P231_Y0.8900.48
211_L233_F0.8890.48
161_V193_I0.8880.48
74_P80_I0.8880.48
67_M194_S0.8870.48
110_D252_S0.8840.47
7_P16_G0.8830.47
7_P15_G0.8830.47
139_N297_K0.8790.47
284_L290_V0.8710.46
252_S334_E0.8710.46
315_E331_I0.8680.46
90_P324_L0.8680.46
125_L228_E0.8630.46
158_A241_L0.8590.45
262_A288_I0.8580.45
26_R242_S0.8550.45
325_A329_A0.8540.45
44_A236_C0.8530.45
206_A241_L0.8520.45
23_P31_V0.8470.45
160_A170_D0.8460.44
268_L333_P0.8420.44
29_A51_P0.8400.44
208_A232_T0.8400.44
64_R86_A0.8350.44
27_L269_V0.8340.44
17_Y223_R0.8330.43
26_R107_L0.8330.43
294_R314_V0.8320.43
79_R155_G0.8320.43
137_I167_D0.8310.43
122_G136_T0.8240.43
6_V27_L0.8240.43
8_T15_G0.8230.43
8_T16_G0.8230.43
22_L106_T0.8220.43
83_V117_T0.8220.43
78_N298_T0.8200.42
186_L230_S0.8180.42
294_R335_S0.8170.42
36_I57_V0.8170.42
30_S329_A0.8130.42
33_D53_N0.8110.42
295_E333_P0.8070.42
323_V333_P0.8050.41
133_S184_D0.8050.41
93_R97_L0.8040.41
333_P336_L0.8040.41
264_D269_V0.7990.41
211_L214_L0.7980.41
284_L309_I0.7980.41
275_L280_V0.7970.41
189_V229_I0.7930.40
217_D269_V0.7900.40
46_D230_S0.7890.40
6_V22_L0.7870.40
76_R239_K0.7820.40
179_Q260_I0.7770.39
86_A118_D0.7750.39
256_R335_S0.7720.39
173_A177_Y0.7720.39
325_A332_S0.7700.39
62_L264_D0.7670.39
101_D104_R0.7670.39
61_A70_W0.7640.38
149_K326_A0.7620.38
79_R243_R0.7600.38
48_Y125_L0.7600.38
268_L283_A0.7580.38
83_V220_V0.7580.38
315_E318_L0.7550.38
128_S265_V0.7530.37
257_P269_V0.7520.37
277_G305_E0.7500.37
96_H211_L0.7450.37
165_F210_A0.7450.37
158_A237_V0.7450.37
80_I321_A0.7440.37
149_K301_Q0.7430.37
12_A298_T0.7420.37
10_I312_I0.7420.37
168_D186_L0.7390.36
38_H222_P0.7350.36
6_V219_V0.7350.36
113_D156_A0.7350.36
202_Q249_T0.7340.36
291_I314_V0.7340.36
32_C328_R0.7330.36
295_E315_E0.7320.36
214_L257_P0.7310.36
90_P121_L0.7300.36
67_M278_L0.7290.36
119_A306_A0.7270.36
43_N56_Y0.7240.35
276_G292_A0.7220.35
283_A289_P0.7180.35
83_V201_F0.7140.35
80_I301_Q0.7140.35
95_R228_E0.7130.35
26_R331_I0.7110.34
161_V197_I0.7110.34
165_F184_D0.7080.34
56_Y59_G0.7080.34
78_N295_E0.7060.34
82_L111_I0.7040.34
180_G233_F0.7020.34
317_Y320_A0.7020.34
177_Y233_F0.6990.34
272_A298_T0.6980.34
80_I84_L0.6980.34
182_G216_L0.6950.33
52_P145_R0.6920.33
185_P206_A0.6920.33
160_A163_A0.6880.33
55_L78_N0.6880.33
4_L38_H0.6850.33
4_L35_L0.6830.33
29_A304_P0.6810.32
140_P193_I0.6790.32
295_E301_Q0.6780.32
290_V310_K0.6780.32
83_V143_L0.6750.32
27_L30_S0.6750.32
99_A228_E0.6750.32
117_T235_P0.6740.32
99_A235_P0.6690.32
165_F170_D0.6670.31
6_V12_A0.6670.31
153_E290_V0.6670.31
38_H47_L0.6670.31
183_V232_T0.6660.31
85_D162_V0.6640.31
248_V312_I0.6620.31
183_V233_F0.6610.31
85_D222_P0.6600.31
57_V72_L0.6600.31
137_I192_V0.6590.31
73_Q162_V0.6570.31
142_S145_R0.6560.31
89_E164_R0.6560.31
6_V9_G0.6550.31
42_V94_L0.6550.31
263_D268_L0.6530.30
18_A180_G0.6530.30
36_I262_A0.6530.30
63_D194_S0.6520.30
107_L331_I0.6510.30
48_Y184_D0.6500.30
203_I297_K0.6490.30
18_A233_F0.6490.30
185_P299_V0.6460.30
226_A235_P0.6460.30
262_A286_Q0.6460.30
192_V244_A0.6460.30
2_A321_A0.6430.30
61_A198_S0.6420.30
6_V262_A0.6420.30
256_R259_D0.6410.30
44_A47_L0.6360.29
195_H281_L0.6350.29
117_T121_L0.6340.29
3_V66_A0.6340.29
71_G259_D0.6340.29
96_H184_D0.6340.29
275_L311_V0.6340.29
84_L93_R0.6330.29
168_D171_S0.6320.29
256_R328_R0.6300.29
288_I327_L0.6300.29
85_D201_F0.6290.29
272_A304_P0.6280.29
38_H237_V0.6250.29
29_A246_Q0.6250.29
306_A310_K0.6240.29
118_D290_V0.6240.29
177_Y183_V0.6220.28
326_A334_E0.6210.28
229_I236_C0.6190.28
149_K323_V0.6180.28
45_A66_A0.6170.28
137_I273_G0.6140.28
38_H44_A0.6140.28
78_N103_A0.6130.28
276_G281_L0.6120.28
186_L211_L0.6100.28
17_Y84_L0.6070.28
249_T262_A0.6060.27
278_L283_A0.6050.27
280_V290_V0.6050.27
248_V321_A0.6050.27
154_A161_V0.6040.27
166_P170_D0.6030.27
270_I281_L0.6020.27
45_A184_D0.6010.27
137_I145_R0.6000.27
37_T267_A0.6000.27
248_V251_P0.5990.27
200_E294_R0.5990.27
83_V150_L0.5990.27
91_E236_C0.5990.27
76_R197_I0.5990.27
148_E312_I0.5970.27
98_N187_G0.5950.27
105_A335_S0.5930.27
42_V236_C0.5930.27
15_G20_D0.5930.27
16_G20_D0.5930.27
17_Y81_G0.5900.26
102_A105_A0.5880.26
154_A163_A0.5870.26
6_V14_I0.5860.26
203_I287_G0.5860.26
41_V44_A0.5860.26
47_L61_A0.5850.26
221_D288_I0.5850.26
226_A229_I0.5840.26
89_E214_L0.5830.26
67_M282_A0.5820.26
144_L200_E0.5820.26
134_W192_V0.5800.26
184_D235_P0.5790.26
184_D279_P0.5780.26
101_D242_S0.5770.26
97_L219_V0.5770.26
38_H71_G0.5770.26
18_A226_A0.5760.26
46_D184_D0.5740.26
121_L167_D0.5740.26
4_L28_L0.5720.25
229_I261_T0.5710.25
79_R148_E0.5710.25
42_V46_D0.5700.25
129_E305_E0.5700.25
111_I114_Y0.5690.25
101_D235_P0.5690.25
145_R215_P0.5680.25
29_A32_C0.5680.25
85_D211_L0.5680.25
32_C73_Q0.5670.25
159_I192_V0.5670.25
163_A170_D0.5660.25
143_L229_I0.5660.25
202_Q265_V0.5660.25
88_I208_A0.5650.25
64_R159_I0.5640.25
9_G12_A0.5640.25
156_A162_V0.5630.25
131_G276_G0.5610.25
174_L236_C0.5600.25
54_A83_V0.5550.24
252_S335_S0.5540.24
307_L314_V0.5540.24
260_I264_D0.5530.24
11_G210_A0.5530.24
129_E306_A0.5530.24
160_A165_F0.5520.24
25_A234_L0.5520.24
25_A80_I0.5510.24
318_L329_A0.5510.24
67_M290_V0.5510.24
70_W118_D0.5500.24
252_S256_R0.5490.24
97_L331_I0.5490.24
102_A229_I0.5480.24
97_L138_E0.5480.24
278_L298_T0.5480.24
116_V246_Q0.5480.24
79_R181_E0.5470.24
220_V224_A0.5470.24
203_I246_Q0.5460.24
81_G117_T0.5460.24
186_L220_V0.5460.24
77_Q231_Y0.5450.24
96_H210_A0.5450.24
160_A166_P0.5450.24
98_N219_V0.5440.24
47_L107_L0.5430.24
314_V317_Y0.5430.24
32_C324_L0.5410.24
16_G19_G0.5410.24
15_G19_G0.5410.24
4_L268_L0.5390.24
156_A302_V0.5380.23
101_D222_P0.5380.23
169_V182_G0.5380.23
120_P180_G0.5360.23
45_A226_A0.5360.23
200_E246_Q0.5340.23
161_V170_D0.5330.23
113_D116_V0.5310.23
25_A29_A0.5300.23
313_E331_I0.5290.23
254_M261_T0.5280.23
258_G264_D0.5270.23
197_I253_A0.5250.23
216_L224_A0.5240.23
37_T281_L0.5230.23
253_A257_P0.5230.23
217_D224_A0.5230.23
70_W198_S0.5220.23
279_P283_A0.5210.23
122_G139_N0.5190.22
129_E148_E0.5160.22
160_A237_V0.5160.22
89_E118_D0.5150.22
141_D148_E0.5150.22
159_I167_D0.5150.22
114_Y139_N0.5140.22
149_K269_V0.5120.22
197_I336_L0.5120.22
57_V61_A0.5100.22
279_P282_A0.5090.22
301_Q334_E0.5090.22
13_E17_Y0.5080.22
2_A31_V0.5070.22
253_A256_R0.5070.22
126_R136_T0.5060.22
162_V165_F0.5060.22
27_L31_V0.5060.22
103_A248_V0.5040.22
149_K181_E0.5030.22
37_T243_R0.5000.21
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4ry0A 1 0.7033 24.4 0.978 Contact Map
3ojcA 4 0.6231 21.6 0.978 Contact Map
1tv5A 3 0.5875 19.1 0.979 Contact Map
3l6uA 2 0.6944 17.6 0.979 Contact Map
2fvyA 1 0.727 17.2 0.979 Contact Map
2rjoA 1 0.724 16.6 0.98 Contact Map
2hqbA 1 0.6677 15.3 0.98 Contact Map
2ioyA 1 0.6914 14.2 0.98 Contact Map
2dgdA 4 0.6083 14 0.98 Contact Map
2x7xA 2 0.6914 13.4 0.98 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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