GREMLIN Database
DUF3325 - Protein of unknown function (DUF3325)
PFAM: PF11804 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 101 (94)
Sequences: 1777 (1367)
Seq/√Len: 141.0
META: 0.625

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
18_M21_H3.6351.00
58_G62_G3.6101.00
50_L63_L3.3821.00
3_A53_C2.9121.00
59_W63_L2.5691.00
57_D65_L2.4021.00
49_S66_W2.0871.00
84_R87_W2.0511.00
54_V59_W2.0431.00
54_V62_G1.9051.00
68_G71_S1.9011.00
66_W70_L1.9001.00
22_H35_R1.8381.00
18_M24_Q1.7691.00
32_P36_R1.7331.00
22_H26_L1.7291.00
8_Y80_L1.7101.00
16_L25_V1.6971.00
10_G42_G1.6811.00
77_V88_L1.5271.00
22_H34_R1.5271.00
7_A49_S1.4861.00
14_L18_M1.4811.00
11_F70_L1.4551.00
4_L69_L1.4351.00
42_G46_L1.4041.00
22_H30_P1.3831.00
7_A74_A1.3801.00
15_A81_L1.3321.00
50_L66_W1.3111.00
21_H24_Q1.2621.00
8_Y88_L1.2591.00
14_L42_G1.2521.00
51_A54_V1.2501.00
40_L44_L1.2231.00
60_S63_L1.2211.00
53_C69_L1.2071.00
64_V78_V1.0910.99
73_A77_V1.0720.99
6_L52_L1.0630.99
13_A45_L1.0540.99
34_R37_L1.0530.99
28_R34_R1.0500.99
50_L54_V1.0230.99
57_D64_V1.0040.98
14_L17_A1.0010.98
61_M65_L0.9850.98
67_L70_L0.9790.98
33_R37_L0.9720.98
12_A77_V0.9720.98
37_L40_L0.9710.98
18_M61_M0.9690.98
33_R36_R0.9590.98
10_G38_L0.9290.98
4_L8_Y0.9150.97
79_L82_S0.8850.97
61_M64_V0.8750.96
7_A69_L0.8680.96
59_W83_Y0.8470.96
11_F74_A0.8470.96
92_A95_A0.8430.96
10_G45_L0.8360.95
11_F68_G0.8350.95
19_D23_R0.8240.95
30_P35_R0.8200.95
75_L79_L0.8170.95
86_R93_A0.8080.94
23_R29_P0.7990.94
75_L78_V0.7940.94
15_A21_H0.7940.94
17_A43_W0.7840.94
81_L88_L0.7630.93
13_A18_M0.7630.93
40_L55_A0.7610.92
57_D62_G0.7610.92
6_L45_L0.7590.92
11_F77_V0.7530.92
15_A71_S0.7450.92
7_A11_F0.7370.91
12_A88_L0.7280.91
11_F73_A0.7270.91
3_A52_L0.7250.91
18_M39_R0.7170.90
2_L80_L0.6980.89
90_P95_A0.6870.88
22_H68_G0.6760.87
8_Y74_A0.6690.87
67_L82_S0.6550.86
11_F24_Q0.6540.85
23_R27_G0.6530.85
36_R40_L0.6510.85
21_H81_L0.6500.85
13_A38_L0.6490.85
5_L95_A0.6460.85
20_R58_G0.6430.85
6_L42_G0.6420.84
14_L60_S0.6420.84
31_S36_R0.6340.84
57_D61_M0.6320.84
3_A65_L0.6310.83
17_A39_R0.6240.83
16_L23_R0.6240.83
50_L59_W0.6230.83
28_R76_L0.6190.82
5_L8_Y0.6170.82
39_R43_W0.6140.82
9_A50_L0.6110.81
5_L9_A0.6060.81
60_S82_S0.6010.80
90_P93_A0.5940.80
28_R31_S0.5830.78
64_V73_A0.5820.78
8_Y69_L0.5660.76
53_C65_L0.5600.76
91_L94_A0.5530.75
15_A77_V0.5490.74
24_Q52_L0.5450.74
36_R43_W0.5440.73
54_V63_L0.5430.73
9_A71_S0.5410.73
12_A79_L0.5410.73
80_L83_Y0.5400.73
35_R39_R0.5400.73
89_R94_A0.5370.72
17_A40_L0.5320.72
78_V82_S0.5270.71
61_M68_G0.5270.71
9_A45_L0.5240.71
13_A41_A0.5230.71
24_Q82_S0.5230.71
29_P79_L0.5230.71
10_G14_L0.5220.70
74_A77_V0.5220.70
87_W90_P0.5180.70
46_L60_S0.5160.70
80_L88_L0.5140.69
33_R89_R0.5120.69
50_L70_L0.5100.69
61_M80_L0.5080.68
90_P94_A0.5020.67
86_R89_R0.5000.67
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4p6vD 1 0.7129 19.5 0.859 Contact Map
2xq2A 2 0.9802 4.6 0.894 Contact Map
4od4A 1 0.9208 3.9 0.897 Contact Map
3dh4A 3 0.9802 3.8 0.899 Contact Map
4tq3A 1 0.9208 3.4 0.901 Contact Map
2onkC 2 0.6337 3.4 0.901 Contact Map
4nv5A 1 0.9307 2.9 0.904 Contact Map
4gx0A 3 0.6634 2.8 0.905 Contact Map
3rkoJ 1 0.2574 2.4 0.909 Contact Map
4he8J 1 0.198 2.4 0.909 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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