GREMLIN Database
DUF3322 - Uncharacterized protein conserved in bacteria N-term (DUF3322)
PFAM: PF11795 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 190 (182)
Sequences: 2612 (2107)
Seq/√Len: 156.2
META: 0.828

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
86_F95_L4.4041.00
45_D48_A4.0241.00
64_R87_D3.5921.00
115_R153_A3.1771.00
93_L102_A3.1641.00
31_R83_R3.0061.00
69_E79_R2.9951.00
66_E83_R2.9821.00
68_R82_A2.7341.00
69_E77_R2.6171.00
88_T91_D2.4691.00
118_A156_R2.4591.00
137_W188_L2.3331.00
98_K101_E2.2871.00
9_R13_D2.1301.00
57_R79_R2.1251.00
4_I32_L1.9051.00
66_E85_W1.9011.00
168_L178_I1.8641.00
170_I173_V1.8611.00
115_R150_A1.8351.00
111_L146_P1.8201.00
147_R174_D1.7861.00
47_D50_R1.7731.00
38_T41_E1.7611.00
67_W79_R1.7411.00
21_L102_A1.7401.00
47_D51_A1.7361.00
64_R85_W1.7261.00
52_W56_L1.7081.00
11_R30_L1.7011.00
133_L184_L1.6791.00
109_A113_R1.6521.00
99_E103_R1.6241.00
113_R120_L1.5961.00
136_D181_H1.5811.00
7_Q33_P1.5671.00
151_V170_I1.5661.00
153_A157_A1.5601.00
20_A25_E1.5531.00
71_N77_R1.5391.00
65_V84_I1.5061.00
94_A99_E1.4921.00
104_R134_A1.4911.00
116_F153_A1.4871.00
163_L167_Q1.4831.00
8_L96_I1.4451.00
186_A190_D1.4061.00
154_W168_L1.3911.00
59_W63_Y1.3591.00
110_E114_A1.3551.00
86_F92_A1.3411.00
50_R54_A1.3331.00
146_P150_A1.3271.00
90_D99_E1.3061.00
53_I75_L1.2971.00
5_R9_R1.2871.00
53_I67_W1.2771.00
21_L106_R1.2751.00
154_W158_H1.2721.00
49_V52_W1.2521.00
89_L93_L1.2501.00
148_L184_L1.2451.00
89_L106_R1.2311.00
129_R187_E1.2091.00
16_E19_R1.2081.00
129_R132_E1.2051.00
31_R66_E1.1951.00
116_F152_V1.1761.00
54_A57_R1.1741.00
54_A58_A1.1741.00
151_V173_V1.1721.00
5_R95_L1.1171.00
120_L124_A1.1121.00
6_A10_R1.1111.00
178_I185_L1.1071.00
72_H75_L1.1000.99
51_A54_A1.0830.99
164_Y167_Q1.0830.99
8_L92_A1.0770.99
154_W161_P1.0730.99
63_Y91_D1.0590.99
89_L102_A1.0550.99
18_L105_F1.0470.99
123_L130_L1.0410.99
60_S63_Y1.0280.99
6_A9_R1.0200.99
151_V168_L1.0160.99
104_R107_A1.0150.99
22_L109_A1.0060.99
8_L95_L0.9970.99
7_Q11_R0.9940.99
147_R150_A0.9940.99
182_R186_A0.9870.99
136_D147_R0.9730.99
174_D180_R0.9690.99
104_R108_L0.9640.99
57_R67_W0.9600.99
126_R129_R0.9480.98
116_F156_R0.9390.98
112_T149_L0.9380.98
2_A55_A0.9310.98
40_A44_E0.9290.98
61_K91_D0.9260.98
56_L60_S0.9190.98
56_L63_Y0.9150.98
42_L49_V0.8980.98
158_H161_P0.8940.98
165_L175_T0.8920.98
112_T137_W0.8910.98
7_Q32_L0.8830.98
126_R187_E0.8810.98
17_L27_F0.8790.97
155_L189_L0.8780.97
87_D91_D0.8700.97
112_T130_L0.8700.97
57_R62_E0.8660.97
57_R65_V0.8660.97
31_R85_W0.8580.97
150_A170_I0.8560.97
155_L163_L0.8530.97
112_T123_L0.8370.97
46_F50_R0.8330.96
75_L78_Q0.8300.96
181_H185_L0.8290.96
11_R14_R0.8260.96
12_W39_A0.8210.96
41_E45_D0.8180.96
108_L112_T0.8160.96
53_I81_P0.8110.96
183_G187_E0.8080.96
42_L75_L0.7950.95
56_L61_K0.7820.95
103_R107_A0.7790.95
147_R173_V0.7790.95
71_N74_V0.7730.95
49_V75_L0.7660.94
155_L170_I0.7600.94
70_V80_L0.7570.94
106_R110_E0.7550.94
4_I84_I0.7530.94
36_G68_R0.7460.94
165_L177_F0.7460.94
4_I56_L0.7390.93
33_P83_R0.7390.93
42_L72_H0.7390.93
65_V81_P0.7390.93
151_V155_L0.7390.93
53_I80_L0.7380.93
164_Y177_F0.7280.93
3_D6_A0.7250.93
166_R176_K0.7250.93
52_W154_W0.7170.92
20_A26_P0.7160.92
108_L134_A0.7100.92
159_P163_L0.7050.92
10_R19_R0.7050.92
23_T124_A0.7030.91
175_T179_E0.6970.91
171_P175_T0.6970.91
63_Y84_I0.6910.91
150_A172_G0.6910.91
177_F181_H0.6900.91
119_L164_Y0.6890.91
17_L92_A0.6790.90
108_L135_D0.6780.90
130_L137_W0.6770.90
17_L93_L0.6700.89
43_L74_V0.6680.89
167_Q170_I0.6650.89
163_L168_L0.6640.89
66_E82_A0.6620.89
57_R75_L0.6600.89
105_F108_L0.6510.88
51_A55_A0.6510.88
102_A106_R0.6500.88
52_W76_G0.6470.88
32_L86_F0.6460.88
104_R135_D0.6440.88
52_W167_Q0.6440.88
133_L181_H0.6440.88
93_L98_K0.6430.87
10_R13_D0.6420.87
40_A43_L0.6400.87
165_L178_I0.6390.87
57_R60_S0.6390.87
52_W57_R0.6340.87
150_A153_A0.6330.87
127_P131_L0.6330.87
19_R78_Q0.6300.86
116_F119_L0.6260.86
112_T188_L0.6240.86
34_L37_P0.6240.86
19_R23_T0.6220.86
43_L46_F0.6210.86
36_G56_L0.6210.86
111_L115_R0.6190.85
84_I95_L0.6180.85
112_T148_L0.6160.85
17_L21_L0.6150.85
167_Q179_E0.6150.85
39_A72_H0.6140.85
185_L189_L0.6120.85
4_I7_Q0.6110.85
49_V78_Q0.6070.84
100_R103_R0.6010.84
7_Q30_L0.5920.83
3_D7_Q0.5920.83
109_A120_L0.5920.83
34_L84_I0.5900.83
122_W126_R0.5890.82
20_A27_F0.5880.82
108_L130_L0.5860.82
153_A156_R0.5840.82
21_L89_L0.5840.82
12_W72_H0.5830.82
46_F49_V0.5830.82
150_A171_P0.5830.82
148_L185_L0.5820.82
111_L135_D0.5770.81
174_D177_F0.5760.81
119_L187_E0.5720.81
98_K105_F0.5700.80
111_L149_L0.5690.80
66_E149_L0.5680.80
105_F131_L0.5640.80
108_L131_L0.5640.80
116_F149_L0.5640.80
9_R98_K0.5620.79
173_V177_F0.5610.79
4_I175_T0.5590.79
2_A25_E0.5580.79
159_P167_Q0.5570.79
24_G48_A0.5540.78
90_D106_R0.5510.78
154_W169_P0.5460.77
78_Q152_V0.5330.76
28_F92_A0.5330.76
169_P174_D0.5320.76
8_L12_W0.5320.76
63_Y86_F0.5300.75
72_H78_Q0.5290.75
30_L78_Q0.5280.75
127_P137_W0.5280.75
109_A130_L0.5250.75
173_V179_E0.5240.75
12_W15_G0.5240.75
128_L184_L0.5230.74
41_E44_E0.5220.74
101_E121_P0.5220.74
36_G39_A0.5200.74
68_R114_A0.5190.74
22_L124_A0.5170.74
3_D110_E0.5170.74
37_P41_E0.5160.73
39_A73_R0.5160.73
41_E75_L0.5150.73
170_I178_I0.5140.73
63_Y78_Q0.5130.73
6_A13_D0.5090.72
76_G102_A0.5090.72
168_L174_D0.5070.72
165_L174_D0.5060.72
119_L122_W0.5060.72
81_P167_Q0.5050.72
92_A95_L0.5050.72
186_A189_L0.5050.72
74_V162_G0.5040.72
38_T107_A0.5030.72
74_V130_L0.5020.71
4_I63_Y0.5010.71
148_L188_L0.5010.71
81_P173_V0.5000.71
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2yd0A 1 0.9105 27.2 0.929 Contact Map
4jbsA 1 0.9105 22.5 0.932 Contact Map
3rjoA 1 0.9 22.4 0.932 Contact Map
4pj6A 2 0.9053 12.5 0.939 Contact Map
4kx7A 1 0.9105 10.5 0.941 Contact Map
4jc0A 1 0.9368 5.6 0.948 Contact Map
2b4jC 1 0.0947 5.4 0.949 Contact Map
3r84A 2 0.4 4.7 0.95 Contact Map
2qgqA 1 0.8789 4.4 0.951 Contact Map
4kmoB 1 0.4421 4 0.952 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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