GREMLIN Database
DUF3316 - Protein of unknown function (DUF3316)
PFAM: PF11777 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 145 (135)
Sequences: 815 (686)
Seq/√Len: 59.1
META: 0.887

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
56_L96_P5.2421.00
112_G130_L4.1511.00
48_I93_T3.6301.00
95_N99_N3.0541.00
100_A142_R2.7781.00
42_G64_R2.7691.00
43_I61_T2.7291.00
112_G129_G2.5971.00
84_Q109_Y2.5971.00
39_T65_F2.4681.00
94_K98_G2.3211.00
105_G137_F2.2561.00
86_F107_Y2.1741.00
62_G89_D1.9611.00
104_S138_I1.8811.00
120_D123_L1.8591.00
82_R111_Y1.8070.99
37_R69_R1.8000.99
67_Y84_Q1.7140.99
47_N59_T1.7110.99
47_N57_K1.6990.99
58_Y95_N1.6880.99
73_T76_A1.5940.99
92_S103_L1.5710.99
16_L19_Q1.5110.98
5_L8_L1.4780.98
106_F134_N1.4630.98
46_T91_S1.4520.98
39_T67_Y1.4320.97
15_P28_S1.3660.96
102_E140_N1.3640.96
81_S116_R1.3620.96
27_D30_E1.3260.96
8_L14_L1.3010.95
81_S115_Y1.3010.95
40_M66_L1.2720.95
55_P58_Y1.2650.94
100_A143_N1.2650.94
116_R124_K1.2310.94
39_T109_Y1.2240.93
90_L103_L1.2230.93
73_T113_L1.2200.93
44_G51_T1.2020.93
69_R84_Q1.1910.92
80_W115_Y1.1820.92
81_S114_H1.1810.92
48_I138_I1.1800.92
92_S101_S1.1720.92
92_S97_A1.1550.91
108_N134_N1.1520.91
70_M83_Q1.1510.91
51_T90_L1.1420.91
56_L100_A1.1350.91
94_K101_S1.1090.89
139_Y145_N1.0790.88
95_N100_A1.0710.88
98_G101_S1.0390.86
37_R67_Y1.0380.86
26_A30_E1.0290.86
35_V69_R1.0190.85
110_S132_D1.0130.85
54_S143_N1.0120.85
112_G116_R0.9810.83
80_W113_L0.9790.83
39_T68_E0.9700.82
7_L11_L0.9670.82
10_L43_I0.9630.82
68_E85_I0.9630.82
85_I114_H0.9560.81
43_I124_K0.9470.81
58_Y110_S0.9460.80
51_T136_G0.9420.80
11_L17_F0.9220.79
58_Y142_R0.9070.78
63_L141_T0.8960.77
99_N116_R0.8920.76
65_F68_E0.8840.76
7_L14_L0.8840.76
61_T71_R0.8610.74
46_T93_T0.8530.73
117_F127_A0.8440.72
95_N113_L0.8440.72
64_R106_F0.8440.72
99_N144_S0.8320.71
19_Q27_D0.8300.71
119_P123_L0.8240.70
83_Q114_H0.8230.70
102_E138_I0.8170.70
41_I65_F0.8130.69
44_G139_Y0.8130.69
65_F89_D0.8120.69
90_L132_D0.8100.69
82_R113_L0.8040.69
83_Q86_F0.7930.68
141_T144_S0.7920.67
49_L91_S0.7900.67
46_T138_I0.7830.67
99_N142_R0.7810.66
85_I110_S0.7810.66
56_L107_Y0.7810.66
114_H126_L0.7760.66
86_F109_Y0.7760.66
49_L54_S0.7740.66
54_S142_R0.7720.65
74_R77_N0.7590.64
35_V39_T0.7560.64
58_Y100_A0.7540.64
36_T138_I0.7480.63
29_L32_N0.7470.63
108_N132_D0.7450.63
37_R41_I0.7420.62
75_L83_Q0.7420.62
53_L128_G0.7400.62
68_E83_Q0.7370.62
38_S133_A0.7350.62
38_S70_M0.7330.61
14_L18_A0.7300.61
27_D31_A0.7280.61
63_L90_L0.7260.61
8_L16_L0.7210.60
95_N142_R0.7160.60
81_S126_L0.7140.59
68_E86_F0.7050.58
11_L26_A0.7040.58
39_T69_R0.7030.58
127_A137_F0.7030.58
5_L12_L0.7010.58
9_L15_P0.7000.58
67_Y107_Y0.6960.57
44_G70_M0.6920.57
140_N143_N0.6890.57
39_T76_A0.6880.57
93_T102_E0.6860.56
115_Y140_N0.6840.56
46_T66_L0.6840.56
66_L85_I0.6820.56
47_N137_F0.6800.56
88_A103_L0.6800.56
121_P126_L0.6770.55
109_Y133_A0.6740.55
53_L143_N0.6730.55
46_T103_L0.6670.54
9_L12_L0.6670.54
131_A141_T0.6650.54
34_P42_G0.6610.54
48_I96_P0.6610.54
91_S104_S0.6610.54
93_T100_A0.6550.53
10_L13_S0.6510.52
62_G106_F0.6490.52
9_L19_Q0.6440.52
27_D79_R0.6420.51
124_K127_A0.6400.51
28_S31_A0.6340.51
33_R118_R0.6340.51
9_L27_D0.6300.50
62_G81_S0.6290.50
64_R87_Q0.6290.50
138_I144_S0.6240.49
7_L15_P0.6210.49
5_L11_L0.6130.48
96_P102_E0.6090.48
6_L11_L0.5990.47
37_R78_G0.5870.45
54_S105_G0.5850.45
91_S132_D0.5790.44
27_D43_I0.5770.44
75_L80_W0.5750.44
103_L131_A0.5710.44
49_L123_L0.5680.43
12_L18_A0.5670.43
9_L14_L0.5570.42
10_L17_F0.5570.42
90_L106_F0.5570.42
105_G127_A0.5550.42
11_L127_A0.5540.42
52_Y60_G0.5540.42
64_R109_Y0.5520.42
5_L9_L0.5480.41
34_P37_R0.5450.41
72_M116_R0.5410.40
37_R53_L0.5410.40
12_L28_S0.5410.40
44_G144_S0.5400.40
90_L104_S0.5380.40
62_G66_L0.5380.40
30_E51_T0.5360.40
63_L105_G0.5320.39
6_L17_F0.5300.39
8_L15_P0.5300.39
60_G128_G0.5270.39
80_W83_Q0.5270.39
120_D133_A0.5250.39
13_S30_E0.5230.38
6_L14_L0.5200.38
36_T103_L0.5170.38
42_G115_Y0.5170.38
36_T134_N0.5170.38
46_T139_Y0.5130.37
89_D106_F0.5090.37
16_L93_T0.5070.37
68_E97_A0.5000.36
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3nb3A 2 0.6207 86.1 0.882 Contact Map
2k0lA 1 0.7793 21.8 0.926 Contact Map
2f2fC 1 0.6483 6 0.943 Contact Map
2q1fA 1 0.731 5.8 0.943 Contact Map
1vw4F 1 0.4966 5 0.945 Contact Map
4fqeA 1 0.4828 3 0.95 Contact Map
4ymxA 1 0.6207 2.8 0.951 Contact Map
3zbiC 1 0.1931 2.3 0.953 Contact Map
2wjrA 1 0.4966 1.6 0.957 Contact Map
2jenA 5 0.6759 1.6 0.958 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0062 seconds.