GREMLIN Database
DUF3300 - Protein of unknown function (DUF3300)
PFAM: PF11737 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 227 (212)
Sequences: 10073 (5327)
Seq/√Len: 365.9
META: 0.961

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
94_Q97_D4.4711.00
110_Q125_T3.2821.00
49_D53_D3.2621.00
6_D104_R3.2351.00
170_Y173_G2.9661.00
75_K79_D2.5831.00
37_A78_A2.2471.00
34_V38_R2.1311.00
174_I177_G2.0721.00
107_A111_A2.0351.00
199_V203_V2.0301.00
124_T131_V2.0061.00
33_V78_A1.9331.00
200_N203_V1.9011.00
101_A104_R1.8901.00
4_Q86_D1.8761.00
126_E131_V1.8731.00
37_A41_Q1.8691.00
53_D56_D1.8601.00
176_F182_V1.8581.00
178_A181_A1.8491.00
21_A25_M1.8401.00
181_A184_A1.8291.00
9_V105_L1.8191.00
174_I182_V1.8151.00
110_Q116_K1.7991.00
201_I205_R1.7901.00
9_V101_A1.6621.00
76_M80_N1.6261.00
172_A175_G1.6211.00
31_L34_V1.5851.00
202_N205_R1.5611.00
41_Q75_K1.5401.00
55_V68_V1.5371.00
37_A75_K1.5221.00
177_G180_I1.5161.00
54_A57_K1.5131.00
180_I184_A1.5071.00
91_F95_Q1.5031.00
28_T92_L1.4951.00
6_D101_A1.4911.00
85_Q89_D1.4621.00
117_S122_K1.4551.00
177_G182_V1.4501.00
107_A130_I1.4451.00
20_L23_V1.4411.00
9_V98_V1.4381.00
50_A53_D1.3981.00
118_T121_Q1.3901.00
114_N150_V1.3851.00
208_N211_R1.3771.00
172_A176_F1.3541.00
38_R78_A1.3491.00
169_Y172_A1.3481.00
52_A69_A1.3451.00
121_Q125_T1.3301.00
41_Q44_P1.3121.00
55_V65_K1.3081.00
9_V87_L1.3001.00
59_P65_K1.2991.00
165_P169_Y1.2971.00
48_G51_L1.2961.00
12_I20_L1.2931.00
203_V206_N1.2871.00
185_G188_G1.2861.00
178_A182_V1.2691.00
108_K111_A1.2661.00
189_W192_C1.2621.00
36_A74_L1.2611.00
100_D104_R1.2591.00
47_K50_A1.2441.00
175_G178_A1.2361.00
20_L102_V1.2321.00
193_G196_G1.2241.00
124_T133_E1.2191.00
110_Q130_I1.2141.00
4_Q7_Q1.2081.00
56_D65_K1.2011.00
24_L102_V1.1911.00
31_L38_R1.1871.00
35_E38_R1.1861.00
34_V81_L1.1851.00
205_R208_N1.1851.00
160_P163_Y1.1781.00
96_A100_D1.1771.00
33_V84_T1.1761.00
122_K135_A1.1751.00
201_I204_N1.1731.00
122_K133_E1.1711.00
107_A110_Q1.1651.00
40_L74_L1.1641.00
148_T152_G1.1621.00
163_Y166_P1.1581.00
2_P104_R1.1571.00
188_G191_G1.1471.00
49_D52_A1.1471.00
35_E82_D1.1401.00
125_T128_Q1.1381.00
166_P169_Y1.1351.00
18_P134_P1.1331.00
19_L142_V1.1311.00
82_D86_D1.1311.00
200_N204_N1.1271.00
199_V202_N1.1181.00
39_W54_A1.1151.00
72_D75_K1.1091.00
5_L9_V1.1091.00
174_I178_A1.1041.00
210_N213_N1.1041.00
2_P101_A1.1041.00
31_L81_L1.1021.00
89_D92_L1.0841.00
37_A40_L1.0781.00
209_F212_N1.0751.00
55_V69_A1.0731.00
187_G191_G1.0701.00
38_R81_L1.0481.00
46_L55_V1.0461.00
6_D108_K1.0451.00
32_E35_E1.0421.00
162_Y165_P1.0341.00
161_P164_Y1.0251.00
52_A55_V1.0131.00
43_N58_Q1.0061.00
175_G180_I1.0051.00
207_N210_N1.0031.00
108_K155_P0.9951.00
159_Y162_Y0.9921.00
89_D93_A0.9881.00
207_N213_N0.9881.00
26_A64_V0.9871.00
180_I183_G0.9821.00
192_C197_G0.9801.00
168_G171_A0.9681.00
117_S123_V0.9661.00
86_D89_D0.9651.00
206_N210_N0.9601.00
186_G189_W0.9591.00
70_F134_P0.9521.00
27_S77_M0.9501.00
52_A56_D0.9461.00
198_W201_I0.9431.00
97_D100_D0.9431.00
177_G181_A0.9381.00
53_D57_K0.9361.00
162_Y166_P0.9311.00
195_G198_W0.9251.00
207_N211_R0.9241.00
208_N212_N0.9241.00
176_F180_I0.8961.00
202_N206_N0.8961.00
70_F73_V0.8951.00
36_A68_V0.8911.00
165_P168_G0.8891.00
23_V73_V0.8811.00
202_N208_N0.8801.00
104_R108_K0.8751.00
164_Y167_P0.8731.00
15_Y23_V0.8671.00
161_P165_P0.8591.00
11_P80_N0.8581.00
168_G172_A0.8571.00
31_L35_E0.8571.00
192_C198_W0.8421.00
167_P170_Y0.8341.00
187_G192_C0.8341.00
175_G181_A0.8321.00
206_N211_R0.8231.00
209_F213_N0.8231.00
41_Q78_A0.8221.00
157_P162_Y0.8171.00
5_L90_A0.8161.00
203_V208_N0.8081.00
23_V67_L0.8071.00
200_N205_R0.8021.00
205_R211_R0.7991.00
34_V78_A0.7961.00
99_M102_V0.7931.00
40_L68_V0.7891.00
174_I184_A0.7871.00
178_A184_A0.7871.00
73_V142_V0.7851.00
193_G197_G0.7851.00
175_G182_V0.7731.00
6_D155_P0.7691.00
192_C195_G0.7661.00
22_Q66_A0.7631.00
157_P161_P0.7581.00
36_A64_V0.7571.00
204_N207_N0.7561.00
134_P137_P0.7551.00
175_G183_G0.7511.00
171_A174_I0.7491.00
172_A180_I0.7481.00
6_D9_V0.7461.00
26_A33_V0.7451.00
29_Y32_E0.7391.00
83_W87_L0.7371.00
154_W158_A0.7321.00
205_R209_F0.7291.00
126_E133_E0.7261.00
46_L51_L0.7231.00
43_N46_L0.7221.00
162_Y169_Y0.7211.00
141_Y151_Y0.7191.00
192_C196_G0.7171.00
206_N212_N0.7171.00
186_G190_G0.7120.99
75_K78_A0.7100.99
119_P122_K0.7060.99
62_P65_K0.6970.99
46_L54_A0.6960.99
21_A99_M0.6940.99
172_A182_V0.6920.99
113_G116_K0.6880.99
193_G198_W0.6850.99
3_E6_D0.6800.99
39_W43_N0.6790.99
163_Y169_Y0.6750.99
27_S91_F0.6740.99
175_G184_A0.6680.99
25_M64_V0.6620.99
78_A81_L0.6600.99
199_V206_N0.6600.99
173_G176_F0.6580.99
176_F181_A0.6570.99
105_L108_K0.6560.99
187_G190_G0.6530.99
105_L151_Y0.6520.99
188_G192_C0.6490.99
72_D76_M0.6430.99
176_F179_G0.6390.99
185_G191_G0.6350.99
158_A161_P0.6330.99
156_Y160_P0.6330.99
22_Q62_P0.6320.99
170_Y174_I0.6270.99
81_L84_T0.6270.99
107_A125_T0.6240.99
93_A97_D0.6240.99
203_V207_N0.6230.99
146_N149_V0.6190.99
198_W202_N0.6160.98
45_N50_A0.6150.98
199_V204_N0.6150.98
204_N210_N0.6120.98
6_D105_L0.6120.98
206_N209_F0.6080.98
22_Q70_F0.6070.98
97_D101_A0.6040.98
207_N212_N0.6040.98
154_W157_P0.6030.98
38_R41_Q0.6000.98
96_A197_G0.5980.98
174_I180_I0.5950.98
41_Q79_D0.5940.98
32_E61_D0.5930.98
153_P157_P0.5910.98
164_Y168_G0.5910.98
199_V205_R0.5870.98
206_N213_N0.5870.98
101_A105_L0.5830.98
171_A176_F0.5820.98
43_N54_A0.5790.98
176_F184_A0.5780.98
52_A62_P0.5760.98
205_R210_N0.5750.98
104_R107_A0.5730.98
33_V67_L0.5700.98
175_G179_G0.5670.97
43_N51_L0.5670.97
27_S84_T0.5660.97
76_M83_W0.5660.97
174_I181_A0.5650.97
42_Q79_D0.5640.97
73_V76_M0.5640.97
101_A155_P0.5630.97
51_L55_V0.5620.97
18_P133_E0.5620.97
3_E7_Q0.5560.97
186_G191_G0.5560.97
34_V84_T0.5550.97
8_L86_D0.5540.97
82_D85_Q0.5540.97
182_V187_G0.5520.97
171_A175_G0.5520.97
31_L95_Q0.5520.97
197_G200_N0.5490.97
203_V209_F0.5440.97
158_A162_Y0.5440.97
114_N151_Y0.5430.97
136_N139_V0.5430.97
59_P82_D0.5400.97
26_A77_M0.5380.96
160_P164_Y0.5380.96
2_P97_D0.5370.96
158_A163_Y0.5360.96
204_N208_N0.5340.96
38_R42_Q0.5330.96
5_L101_A0.5310.96
55_V67_L0.5310.96
51_L54_A0.5300.96
185_G189_W0.5300.96
172_A184_A0.5300.96
182_V185_G0.5270.96
178_A183_G0.5270.96
201_I208_N0.5270.96
170_Y176_F0.5260.96
159_Y163_Y0.5210.96
18_P22_Q0.5170.96
12_I87_L0.5170.96
81_L85_Q0.5160.96
201_I206_N0.5130.95
185_G193_G0.5120.95
187_G193_G0.5110.95
148_T153_P0.5110.95
201_I207_N0.5080.95
122_K125_T0.5000.95
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4nf0A 2 0.3656 19.6 0.963 Contact Map
2lqjA 1 0.141 14.9 0.965 Contact Map
3k1rB 1 0.326 11.4 0.967 Contact Map
1mliA 5 0.163 8.7 0.969 Contact Map
4mijA 1 0.4405 7.6 0.97 Contact Map
1wwvA 1 0.3877 6.9 0.97 Contact Map
2qkqA 1 0.2643 5.8 0.971 Contact Map
3mp6A 1 0.2643 5.7 0.971 Contact Map
1vpyA 1 0.2115 5.1 0.972 Contact Map
4trrA 4 0.2643 4.6 0.973 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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