GREMLIN Database
DUF3296 - Protein of unknown function (DUF3296)
PFAM: PF11726 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 179 (170)
Sequences: 2401 (1819)
Seq/√Len: 139.5
META: 0.578

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
86_L158_L3.8831.00
15_R92_D3.1971.00
18_V154_L3.0891.00
21_V85_L2.9381.00
23_L115_W2.7021.00
14_S92_D2.5171.00
71_W177_G2.4871.00
9_A16_V2.4081.00
76_G79_K2.3721.00
24_R79_K2.2861.00
71_W83_Y2.2821.00
2_I70_V2.1031.00
47_K113_E2.0881.00
15_R91_G2.0471.00
76_G166_T2.0231.00
5_Y68_G2.0031.00
108_A132_F1.9641.00
143_N147_D1.8521.00
73_R83_Y1.8341.00
51_D94_Y1.8051.00
43_S47_K1.7981.00
37_I40_F1.7621.00
18_V86_L1.7521.00
18_V88_F1.6931.00
109_K113_E1.6901.00
75_Q81_P1.6881.00
138_Y157_R1.6551.00
128_G133_P1.6521.00
25_Y36_V1.6401.00
50_A54_R1.6131.00
86_L154_L1.6091.00
156_Y159_S1.5861.00
90_N93_K1.5811.00
12_R66_L1.5761.00
27_K80_G1.5761.00
82_H160_Y1.5611.00
74_E160_Y1.5411.00
103_N106_N1.5381.00
116_A126_Y1.5251.00
38_S42_E1.5101.00
52_L55_K1.5091.00
9_A68_G1.5031.00
12_R68_G1.4981.00
19_V89_L1.4931.00
70_V86_L1.4791.00
74_E84_H1.4781.00
133_P136_P1.4741.00
74_E82_H1.4591.00
94_Y107_L1.4501.00
125_D128_G1.4081.00
84_H160_Y1.3981.00
6_L155_F1.3941.00
13_Y67_V1.3451.00
5_Y9_A1.3261.00
125_D130_V1.3181.00
75_Q80_G1.3101.00
42_E45_K1.3031.00
72_V176_F1.2531.00
3_K7_D1.2231.00
165_R168_G1.2191.00
72_V167_K1.2171.00
121_L126_Y1.1891.00
41_I85_L1.1881.00
19_V87_L1.1831.00
47_K117_S1.1440.99
3_K155_F1.1390.99
82_H163_K1.1360.99
54_R58_R1.1240.99
73_R81_P1.1240.99
146_D149_E1.1150.99
147_D150_K1.1150.99
158_L161_L1.1030.99
160_Y163_K1.0990.99
13_Y66_L1.0990.99
43_S117_S1.0900.99
20_R161_L1.0770.99
46_A50_A1.0730.99
135_N138_Y1.0700.99
7_D151_Y1.0610.99
72_V161_L1.0500.99
164_E167_K1.0470.99
40_F43_S1.0450.99
35_K39_R1.0430.99
2_I162_A1.0420.99
47_K110_R1.0220.99
114_A119_L1.0150.99
22_D82_H1.0140.99
6_L154_L1.0120.99
40_F44_L1.0100.99
75_Q78_S1.0040.98
128_G131_H0.9970.98
166_T170_G0.9890.98
37_I41_I0.9870.98
55_K58_R0.9820.98
130_V133_P0.9800.98
91_G139_Y0.9730.98
63_F89_L0.9720.98
167_K170_G0.9690.98
127_A132_F0.9600.98
166_T169_Y0.9570.98
155_F159_S0.9520.98
43_S46_A0.9500.98
135_N140_L0.9370.98
174_R178_C0.9270.97
121_L125_D0.9240.97
65_C93_K0.9180.97
129_L133_P0.9080.97
29_Y32_D0.8920.97
74_E163_K0.8840.97
9_A12_R0.8820.96
41_I45_K0.8810.96
42_E46_A0.8730.96
138_Y141_N0.8690.96
84_H163_K0.8590.96
10_L16_V0.8540.96
52_L56_L0.8520.96
39_R43_S0.8480.96
54_R57_R0.8360.95
127_A131_H0.8350.95
21_V87_L0.8330.95
123_Y127_A0.8320.95
108_A112_G0.8300.95
144_D150_K0.8290.95
164_E169_Y0.8170.95
168_G171_D0.8160.95
41_I69_Y0.8110.94
106_N110_R0.8030.94
38_S41_I0.8000.94
22_D160_Y0.7970.94
13_Y16_V0.7970.94
107_L110_R0.7970.94
124_E127_A0.7950.94
44_L87_L0.7880.94
16_V88_F0.7880.94
147_D151_Y0.7870.93
170_G175_N0.7850.93
53_K56_L0.7840.93
18_V158_L0.7810.93
4_E7_D0.7780.93
5_Y70_V0.7740.93
44_L111_I0.7640.92
23_L85_L0.7630.92
119_L133_P0.7570.92
138_Y142_R0.7560.92
122_D125_D0.7470.92
113_E117_S0.7430.91
129_L132_F0.7410.91
40_F45_K0.7360.91
121_L127_A0.7340.91
55_K60_K0.7340.91
65_C94_Y0.7320.91
114_A118_A0.7250.90
126_Y131_H0.7200.90
68_G178_C0.7160.90
51_D54_R0.7150.90
18_V157_R0.7130.90
6_L18_V0.7110.89
87_L111_I0.7070.89
76_G81_P0.7020.89
170_G173_M0.7010.89
159_S162_A0.6980.89
146_D150_K0.6960.88
40_F47_K0.6950.88
148_K151_Y0.6920.88
49_K53_K0.6910.88
66_L89_L0.6790.87
137_G142_R0.6750.87
127_A130_V0.6740.87
16_V67_V0.6740.87
174_R177_G0.6730.87
53_K57_R0.6700.87
17_L139_Y0.6680.86
123_Y126_Y0.6680.86
45_K49_K0.6680.86
41_I44_L0.6660.86
168_G175_N0.6630.86
121_L124_E0.6620.86
137_G141_N0.6600.86
20_R82_H0.6580.86
145_P150_K0.6550.85
136_P140_L0.6550.85
36_V40_F0.6540.85
37_I45_K0.6530.85
150_K153_N0.6500.85
44_L47_K0.6450.84
135_N141_N0.6450.84
17_L91_G0.6410.84
141_N144_D0.6390.84
23_L130_V0.6370.84
24_R129_L0.6350.83
142_R145_P0.6350.83
88_F111_I0.6290.83
63_F93_K0.6270.83
28_D31_D0.6260.83
52_L62_V0.6240.82
139_Y144_D0.6220.82
60_K63_F0.6220.82
171_D175_N0.6180.82
43_S50_A0.6150.81
38_S45_K0.6060.81
55_K114_A0.6060.81
22_D161_L0.6040.80
114_A117_S0.6020.80
7_D155_F0.5970.80
122_D128_G0.5930.79
86_L157_R0.5910.79
55_K59_G0.5870.78
136_P139_Y0.5810.78
135_N144_D0.5780.77
11_S32_D0.5740.77
51_D55_K0.5730.77
153_N156_Y0.5650.76
10_L23_L0.5620.75
91_G102_Q0.5600.75
154_L157_R0.5590.75
110_R113_E0.5570.75
83_Y163_K0.5570.75
35_K43_S0.5550.74
126_Y130_V0.5550.74
10_L18_V0.5530.74
37_I47_K0.5520.74
19_V108_A0.5510.74
12_R16_V0.5490.74
165_R171_D0.5460.73
149_E153_N0.5430.73
111_I114_A0.5430.73
47_K51_D0.5430.73
16_V68_G0.5400.72
172_G175_N0.5390.72
104_D108_A0.5390.72
142_R146_D0.5360.72
47_K50_A0.5360.72
72_V178_C0.5350.72
15_R163_K0.5320.71
25_Y119_L0.5310.71
126_Y129_L0.5310.71
163_K167_K0.5260.71
6_L10_L0.5230.70
8_A68_G0.5190.70
30_Q81_P0.5190.70
153_N157_R0.5150.69
113_E116_A0.5140.69
165_R169_Y0.5100.68
6_L88_F0.5080.68
8_A11_S0.5070.68
2_I158_L0.5070.68
57_R60_K0.5060.68
10_L13_Y0.5050.67
41_I47_K0.5050.67
16_V142_R0.5050.67
4_E8_A0.5040.67
48_I67_V0.5030.67
48_I52_L0.5020.67
142_R151_Y0.5020.67
135_N139_Y0.5000.67
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4pp4A 1 0.8547 38.1 0.912 Contact Map
4ht4A 1 0.6648 20.4 0.923 Contact Map
2ns6A 1 0.6201 11.9 0.931 Contact Map
2v6eA 2 0.419 10.6 0.933 Contact Map
2pa4A 3 0.3464 10.5 0.933 Contact Map
4le5A 2 0.2514 6.9 0.938 Contact Map
3fovA 2 0.2402 6 0.94 Contact Map
3dkxA 3 0.7598 5.9 0.94 Contact Map
3h4lA 2 0.905 5.8 0.94 Contact Map
1p7lA 4 0.3352 4.4 0.943 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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