GREMLIN Database
DUF3277 - Protein of unknown function (DUF3277)
PFAM: PF11681 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 144 (135)
Sequences: 1326 (1148)
Seq/√Len: 98.8
META: 0.864

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
36_E120_D4.8951.00
39_E59_D3.4041.00
52_V137_A3.0251.00
101_D104_G2.5571.00
40_D116_Q2.4881.00
44_M135_D2.4051.00
53_M127_G2.3291.00
63_T116_Q2.2061.00
62_G139_I2.2041.00
75_N132_W2.2011.00
67_T131_E2.1681.00
65_T133_V2.0491.00
65_T131_E2.0401.00
38_A61_S2.0331.00
55_S127_G1.9051.00
40_D117_K1.8941.00
113_A136_A1.8501.00
46_V52_V1.7901.00
64_I136_A1.6991.00
124_G128_G1.6991.00
41_K82_Y1.6971.00
60_K86_R1.6661.00
110_A136_A1.6151.00
86_R92_W1.5821.00
44_M61_S1.5481.00
53_M71_T1.4981.00
39_E125_K1.4911.00
46_V61_S1.4841.00
53_M69_L1.4781.00
54_R85_Q1.4701.00
43_T57_S1.4521.00
122_T128_G1.4111.00
44_M52_V1.3910.99
42_N135_D1.3860.99
42_N54_R1.3770.99
36_E65_T1.3610.99
54_R114_A1.3490.99
117_K120_D1.3400.99
112_G137_A1.3310.99
37_R41_K1.3180.99
34_T120_D1.3160.99
76_A105_G1.3030.99
123_Y130_R1.2990.99
116_Q135_D1.2850.99
108_I123_Y1.2690.99
81_L115_F1.2640.99
64_I97_I1.2580.99
43_T126_E1.2440.99
41_K118_P1.2250.99
38_A44_M1.2110.99
45_T69_L1.1810.98
52_V61_S1.1720.98
36_E63_T1.1580.98
67_T129_T1.1570.98
34_T67_T1.1520.98
64_I110_A1.1360.98
39_E57_S1.1300.98
108_I141_S1.1180.98
34_T122_T1.1170.98
30_D125_K1.0550.97
63_T133_V1.0470.96
76_A80_A1.0440.96
66_I134_F1.0370.96
46_V59_D1.0260.96
68_L74_S1.0240.96
57_S125_K1.0210.96
42_N56_L1.0150.96
79_S83_N1.0140.96
44_M114_A0.9890.95
23_G26_A0.9880.95
136_A139_I0.9860.95
38_A63_T0.9770.95
74_S77_L0.9570.94
38_A135_D0.9560.94
97_I134_F0.9440.94
9_V99_I0.9370.93
98_T109_T0.9330.93
41_K86_R0.9310.93
37_R86_R0.9290.93
76_A106_D0.9260.93
37_R62_G0.9210.93
116_Q133_V0.9200.93
108_I121_V0.9150.93
82_Y86_R0.9030.92
37_R60_K0.8980.92
35_I99_I0.8930.92
85_Q93_G0.8740.91
104_G107_L0.8590.90
45_T126_E0.8510.90
97_I113_A0.8490.89
77_L81_L0.8460.89
42_N114_A0.8440.89
97_I136_A0.8440.89
30_D122_T0.8380.89
81_L95_N0.8370.89
63_T135_D0.8350.89
95_N115_F0.8200.88
118_P141_S0.8190.88
82_Y118_P0.8190.88
60_K140_D0.8180.88
11_A99_I0.8170.88
30_D67_T0.8010.87
79_S118_P0.7930.86
37_R118_P0.7890.86
38_A59_D0.7860.85
30_D124_G0.7840.85
117_K133_V0.7790.85
121_V130_R0.7770.85
110_A139_I0.7730.85
21_D24_T0.7720.84
45_T127_G0.7720.84
114_A135_D0.7660.84
84_A91_G0.7660.84
84_A87_A0.7600.84
70_Q128_G0.7590.83
68_L72_S0.7430.82
69_L127_G0.7420.82
99_I108_I0.7420.82
26_A68_L0.7410.82
34_T65_T0.7390.82
47_G69_L0.7370.82
35_I108_I0.7340.81
85_Q88_S0.7290.81
32_G67_T0.7220.80
17_G24_T0.7210.80
35_I123_Y0.7160.80
60_K138_D0.7150.80
40_D43_T0.7140.80
55_S128_G0.6980.78
25_G28_D0.6970.78
51_E138_D0.6970.78
75_N79_S0.6970.78
7_L24_T0.6910.77
45_T129_T0.6900.77
8_D101_D0.6890.77
13_I21_D0.6880.77
115_F134_F0.6870.77
48_A69_L0.6770.76
38_A62_G0.6710.75
35_I64_I0.6680.75
48_A58_A0.6540.73
78_L132_W0.6530.73
51_E69_L0.6500.73
55_S126_E0.6480.73
62_G110_A0.6470.73
100_K107_L0.6450.72
47_G51_E0.6430.72
63_T120_D0.6430.72
62_G108_I0.6420.72
36_E131_E0.6380.71
13_I17_G0.6370.71
45_T53_M0.6370.71
70_Q130_R0.6330.71
37_R117_K0.6330.71
26_A29_G0.6290.70
102_L105_G0.6220.70
61_S135_D0.6080.68
44_M54_R0.6020.67
48_A127_G0.6000.67
122_T126_E0.5880.65
17_G85_Q0.5860.65
77_L80_A0.5860.65
31_E67_T0.5850.65
5_S8_D0.5830.65
42_N92_W0.5820.64
11_A64_I0.5780.64
84_A96_P0.5760.64
82_Y117_K0.5720.63
49_D69_L0.5700.63
95_N113_A0.5680.63
99_I123_Y0.5670.62
88_S91_G0.5670.62
83_N86_R0.5640.62
64_I113_A0.5610.62
13_I97_I0.5610.62
62_G136_A0.5580.61
37_R82_Y0.5550.61
14_S17_G0.5550.61
78_L119_P0.5510.60
52_V136_A0.5500.60
10_S24_T0.5490.60
21_D26_A0.5480.60
54_R93_G0.5430.59
42_N85_Q0.5430.59
98_T107_L0.5410.59
27_G30_D0.5380.58
14_S80_A0.5350.58
40_D133_V0.5350.58
98_T102_L0.5300.57
82_Y115_F0.5260.57
90_A107_L0.5200.56
99_I107_L0.5170.56
67_T122_T0.5160.55
17_G91_G0.5160.55
120_D130_R0.5150.55
45_T55_S0.5150.55
61_S78_L0.5130.55
48_A125_K0.5060.54
33_I108_I0.5020.53
36_E116_Q0.5020.53
71_T127_G0.5010.53
38_A139_I0.5000.53
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2gujA 1 0.875 19.2 0.925 Contact Map
4ljsA 1 0.4167 5.1 0.942 Contact Map
4o66A 2 0.3958 4.6 0.943 Contact Map
4mypA 2 0.5347 4.2 0.944 Contact Map
2o6pA 2 0.5694 3.1 0.948 Contact Map
4ur6A 2 0.2847 3.1 0.948 Contact Map
4kdwA 1 0.6042 3.1 0.948 Contact Map
2wcoA 1 0.4306 2.9 0.949 Contact Map
2ltlA 1 0.3958 2.5 0.95 Contact Map
3qzmA 1 0.6042 2.3 0.951 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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