GREMLIN Database
DUF3000 - Protein of unknown function (DUF3000)
PFAM: PF11452 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 176 (173)
Sequences: 1993 (1107)
Seq/√Len: 84.1
META: 0.689

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
34_P62_D3.9891.00
13_R25_E3.6791.00
108_R161_D3.6201.00
57_L75_L3.5441.00
70_D114_A3.0531.00
95_E127_F2.9471.00
61_H72_T2.7981.00
9_V37_V2.7681.00
70_D113_T2.6141.00
126_S133_R2.5601.00
95_E125_E2.4201.00
113_T148_T2.3701.00
42_D54_T2.1431.00
94_P125_E2.1201.00
116_S129_G2.0831.00
28_A36_A2.0691.00
4_E72_T2.0691.00
21_I164_C2.0401.00
59_L152_L2.0021.00
17_L160_C2.0001.00
44_T49_E1.9861.00
12_L156_L1.9201.00
71_G146_T1.7341.00
45_V48_D1.7291.00
3_A7_A1.7081.00
26_I40_S1.7081.00
97_G139_I1.6561.00
65_G72_T1.6111.00
8_A73_F1.5991.00
122_V126_S1.5931.00
106_D112_Y1.5771.00
79_V166_A1.5771.00
83_L139_I1.5671.00
2_P6_R1.5351.00
103_D106_D1.4990.99
112_Y115_A1.4890.99
160_C164_C1.4880.99
124_S130_L1.4850.99
103_D165_T1.4760.99
4_E61_H1.4630.99
57_L77_T1.4500.99
59_L73_F1.4460.99
94_P121_R1.4330.99
17_L23_L1.4130.99
102_T112_Y1.4040.99
3_A6_R1.4030.99
53_A167_A1.3820.99
12_L37_V1.3810.99
121_R125_E1.3620.99
7_A150_P1.3540.99
62_D66_Q1.3400.99
88_A123_S1.3340.99
11_S59_L1.3170.99
52_L80_R1.2970.98
58_V77_T1.2680.98
80_R138_E1.2570.98
125_E135_D1.2500.98
80_R125_E1.2430.98
84_E87_M1.2390.98
45_V51_E1.2030.97
10_A14_A1.1970.97
111_G149_D1.1960.97
106_D111_G1.1940.97
130_L133_R1.1840.97
110_A154_A1.1730.97
129_G132_G1.1670.97
167_A170_P1.1500.97
96_V99_S1.1490.97
72_T146_T1.1280.96
44_T47_D1.1270.96
122_V138_E1.1270.96
13_R37_V1.1230.96
21_I53_A1.1230.96
40_S126_S1.1190.96
151_D154_A1.1160.96
2_P8_A1.1160.96
116_S137_A1.1050.96
83_L93_L1.1010.96
32_L66_Q1.0810.95
161_D164_C1.0670.95
108_R157_E1.0650.95
77_T143_A1.0620.95
61_H146_T1.0570.95
79_V162_L1.0570.95
118_T124_S1.0550.94
111_G148_T1.0340.94
6_R10_A1.0310.94
40_S78_Y1.0310.94
105_L110_A1.0060.93
41_A163_L1.0000.93
97_G119_V0.9950.93
22_V44_T0.9890.92
37_V75_L0.9870.92
152_L155_H0.9830.92
77_T159_W0.9810.92
99_S104_A0.9800.92
102_T115_A0.9750.92
9_V13_R0.9740.92
150_P153_G0.9610.91
85_P92_L0.9430.90
18_R164_C0.9400.90
8_A153_G0.9290.90
83_L87_M0.9170.89
25_E37_V0.9170.89
42_D78_Y0.9160.89
7_A11_S0.9010.88
116_S133_R0.8920.88
89_A92_L0.8910.88
66_Q147_P0.8860.87
26_I53_A0.8830.87
46_D49_E0.8790.87
97_G113_T0.8720.87
152_L156_L0.8700.86
110_A139_I0.8690.86
17_L32_L0.8670.86
61_H73_F0.8570.86
40_S80_R0.8570.86
58_V66_Q0.8520.85
122_V125_E0.8500.85
58_V138_E0.8470.85
88_A101_L0.8450.85
71_G91_P0.8390.84
87_M115_A0.8290.84
11_S151_D0.8260.84
86_E94_P0.8250.83
8_A11_S0.8240.83
80_R140_E0.8230.83
116_S126_S0.8230.83
126_S159_W0.7920.81
32_L160_C0.7870.80
142_R147_P0.7780.80
96_V100_W0.7710.79
130_L135_D0.7690.79
35_Y63_P0.7580.78
34_P63_P0.7560.78
130_L137_A0.7550.78
77_T156_L0.7550.78
164_C170_P0.7520.77
157_E160_C0.7520.77
157_E172_L0.7460.77
92_L118_T0.7360.76
107_A150_P0.7350.76
11_S150_P0.7260.75
21_I41_A0.7230.75
32_L60_L0.7180.74
137_A159_W0.7170.74
3_A22_V0.7160.74
137_A141_I0.7150.74
156_L164_C0.7110.73
31_R72_T0.7070.73
124_S138_E0.7060.73
7_A10_A0.7040.73
78_Y122_V0.7010.72
65_G71_G0.7000.72
27_P143_A0.6960.72
145_W155_H0.6960.72
80_R122_V0.6930.71
2_P73_F0.6900.71
73_F83_L0.6900.71
84_E123_S0.6860.71
70_D125_E0.6830.70
41_A118_T0.6830.70
57_L90_D0.6820.70
54_T78_Y0.6800.70
66_Q79_V0.6790.70
30_Q36_A0.6780.70
70_D141_I0.6770.70
134_P141_I0.6710.69
69_W74_R0.6680.69
83_L88_A0.6660.68
57_L121_R0.6650.68
32_L137_A0.6540.67
101_L158_A0.6540.67
119_V123_S0.6510.67
73_F116_S0.6500.66
142_R145_W0.6460.66
91_P163_L0.6450.66
2_P35_Y0.6420.66
92_L96_V0.6320.64
85_P129_G0.6250.63
4_E15_A0.6240.63
16_R19_P0.6210.63
106_D109_G0.6210.63
105_L112_Y0.6200.63
65_G128_G0.6200.63
93_L96_V0.6180.63
111_G157_E0.6090.61
81_A101_L0.6080.61
156_L161_D0.6070.61
26_I141_I0.6030.61
48_D51_E0.5980.60
62_D120_T0.5970.60
15_A21_I0.5960.60
107_A161_D0.5930.59
45_V49_E0.5890.59
46_D51_E0.5890.59
109_G139_I0.5850.58
27_P159_W0.5840.58
53_A116_S0.5830.58
22_V32_L0.5810.58
43_V169_L0.5780.58
25_E39_L0.5780.58
66_Q75_L0.5770.57
94_P137_A0.5720.57
115_A119_V0.5690.56
7_A14_A0.5610.55
32_L133_R0.5600.55
144_S147_P0.5600.55
53_A77_T0.5590.55
125_E129_G0.5580.55
25_E70_D0.5580.55
81_A139_I0.5560.55
75_L116_S0.5550.54
4_E73_F0.5530.54
75_L141_I0.5510.54
15_A40_S0.5500.54
32_L118_T0.5500.54
20_E169_L0.5490.54
161_D165_T0.5470.53
89_A121_R0.5460.53
24_E80_R0.5450.53
24_E30_Q0.5450.53
149_D154_A0.5440.53
68_A72_T0.5430.53
57_L156_L0.5390.52
77_T130_L0.5370.52
20_E164_C0.5340.52
94_P115_A0.5320.51
73_F80_R0.5320.51
18_R169_L0.5280.51
15_A160_C0.5270.51
27_P39_L0.5260.51
62_D147_P0.5260.51
26_I32_L0.5260.51
77_T138_E0.5260.51
58_V124_S0.5260.51
10_A132_G0.5250.51
116_S159_W0.5240.50
8_A59_L0.5230.50
93_L139_I0.5230.50
113_T141_I0.5220.50
53_A121_R0.5220.50
48_D151_D0.5200.50
8_A12_L0.5190.50
10_A13_R0.5190.50
42_D146_T0.5180.50
64_A72_T0.5180.50
115_A164_C0.5160.49
66_Q130_L0.5160.49
16_R157_E0.5150.49
53_A122_V0.5150.49
12_L58_V0.5130.49
57_L93_L0.5120.49
13_R26_I0.5120.49
46_D102_T0.5100.49
43_V143_A0.5090.48
160_C166_A0.5080.48
4_E150_P0.5060.48
94_P123_S0.5050.48
26_I133_R0.5000.47
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3emxA 1 0.4091 6.9 0.964 Contact Map
4nzsA 4 0.4602 4.7 0.967 Contact Map
3hlzA 1 0.733 4.2 0.968 Contact Map
2vugA 2 0.2841 4 0.968 Contact Map
2c0cA 1 0.3409 3.4 0.969 Contact Map
4ubtA 3 0.4886 3.3 0.969 Contact Map
4o9aA 4 0.4489 3.3 0.969 Contact Map
3nwmA 1 0.267 3.3 0.969 Contact Map
4j56E 1 0.3239 3.1 0.969 Contact Map
4p56A 1 0.2159 2.9 0.97 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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