GREMLIN Database
DUF3100 - Protein of unknown function (DUF3100)
PFAM: PF11299 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 235 (234)
Sequences: 599 (355)
Seq/√Len: 23.2
META: 0.508

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
110_I230_E5.7041.00
231_W234_K4.7061.00
114_F121_N4.3271.00
87_F220_M4.3021.00
131_L136_G3.6741.00
107_R135_E3.2081.00
11_I18_I3.0411.00
60_L181_G2.8630.99
114_F124_I2.8530.99
170_Y198_M2.8060.99
199_A203_L2.5790.99
176_S187_A2.5470.99
89_N225_A2.5100.98
90_L225_A2.4700.98
181_G185_A2.3520.98
97_L229_T2.3460.98
100_A109_A2.2450.97
102_L106_K2.2410.97
90_L224_I2.1700.96
156_L160_F2.1580.96
188_S192_A2.1220.95
86_E217_G1.9800.93
228_L231_W1.9370.93
74_K201_Q1.8630.91
120_P210_N1.8130.90
128_K226_L1.8110.90
20_L24_L1.7790.89
159_S211_L1.7720.89
117_G139_V1.7410.88
67_L203_L1.7410.88
93_I225_A1.7340.88
148_V211_L1.7340.88
74_K77_A1.7090.87
142_V145_T1.7070.87
92_T174_M1.7050.87
65_G144_I1.6590.85
10_K17_T1.6270.84
84_L89_N1.5960.83
23_M155_G1.5630.82
188_S202_I1.5550.81
92_T213_T1.5430.81
101_L104_G1.5010.79
2_I6_I1.4970.79
60_L70_P1.4770.78
72_L195_Y1.4750.78
178_V181_G1.4590.77
2_I29_I1.4490.76
87_F93_I1.4330.75
54_G59_L1.4240.75
188_S203_L1.4180.75
145_T149_F1.4150.74
209_S213_T1.4140.74
170_Y201_Q1.4090.74
126_G131_L1.3990.74
103_L219_Y1.3980.74
15_P160_F1.3670.72
2_I5_L1.3640.72
188_S206_A1.3620.72
13_L17_T1.3430.70
53_L211_L1.3230.69
117_G213_T1.3210.69
110_I133_S1.3140.69
72_L116_I1.3050.68
101_L140_L1.3040.68
23_M57_L1.2990.68
34_G112_A1.2870.67
34_G122_L1.2850.67
89_N220_M1.2620.66
3_A29_I1.2600.65
113_T181_G1.2590.65
13_L47_K1.2420.64
62_A110_I1.2400.64
97_L113_T1.2290.63
3_A23_M1.2170.63
90_L93_I1.2140.62
192_A197_E1.2100.62
10_K25_Y1.1810.60
63_K149_F1.1810.60
91_G95_L1.1740.60
213_T216_T1.1720.60
55_I156_L1.1520.58
192_A199_A1.1500.58
27_L144_I1.1490.58
216_T220_M1.1320.57
47_K110_I1.1250.56
16_G155_G1.1200.56
95_L229_T1.1150.56
122_L139_V1.1110.56
105_L142_V1.1070.55
83_L224_I1.1040.55
8_T25_Y1.0990.55
189_G193_A1.0810.53
190_A193_A1.0810.53
58_L112_A1.0790.53
112_A172_L1.0650.52
33_L127_E1.0630.52
124_I218_L1.0180.49
157_L228_L1.0110.49
120_P214_G1.0080.48
82_L144_I1.0040.48
191_L202_I0.9990.48
211_L214_G0.9950.47
229_T232_L0.9920.47
129_Y230_E0.9900.47
16_G160_F0.9890.47
101_L106_K0.9890.47
112_A141_G0.9870.47
175_A209_S0.9860.47
139_V147_T0.9780.46
169_P195_Y0.9770.46
96_A113_T0.9710.46
23_M26_A0.9680.46
90_L151_T0.9640.45
27_L64_L0.9610.45
124_I221_S0.9600.45
42_S45_E0.9570.45
28_I148_V0.9570.45
12_P99_V0.9570.45
27_L122_L0.9560.45
71_N79_G0.9560.45
76_I160_F0.9560.45
92_T149_F0.9490.44
114_F221_S0.9490.44
108_E134_P0.9460.44
66_T141_G0.9380.43
83_L87_F0.9380.43
5_L9_I0.9350.43
83_L116_I0.9330.43
10_K201_Q0.9330.43
77_A223_F0.9320.43
116_I151_T0.9270.43
63_K101_L0.9240.43
63_K108_E0.9180.42
24_L28_I0.9160.42
128_K173_A0.9090.42
105_L141_G0.9080.41
121_N221_S0.9030.41
68_V207_A0.9030.41
66_T191_L0.9020.41
126_G136_G0.8990.41
87_F110_I0.8980.41
70_P141_G0.8970.41
94_L215_V0.8960.41
128_K231_W0.8950.41
94_L99_V0.8870.40
23_M182_S0.8870.40
119_E210_N0.8830.40
123_A225_A0.8820.40
24_L147_T0.8790.39
97_L109_A0.8750.39
182_S186_A0.8740.39
124_I128_K0.8700.39
122_L145_T0.8700.39
64_L214_G0.8670.39
53_L159_S0.8650.39
9_I25_Y0.8640.38
185_A207_A0.8630.38
37_K59_L0.8630.38
3_A26_A0.8620.38
220_M224_I0.8560.38
68_V126_G0.8530.38
34_G172_L0.8530.38
95_L228_L0.8520.38
57_L145_T0.8500.38
89_N216_T0.8470.37
20_L73_P0.8470.37
86_E115_S0.8390.37
137_R202_I0.8290.36
3_A8_T0.8260.36
55_I205_F0.8250.36
17_T193_A0.8250.36
155_G231_W0.8200.36
24_L148_V0.8170.35
44_K110_I0.8160.35
66_T70_P0.8140.35
23_M54_G0.8100.35
65_G159_S0.8080.35
9_I20_L0.8070.35
9_I171_A0.8060.35
175_A216_T0.8040.35
83_L171_A0.8000.34
227_P233_Y0.7970.34
60_L221_S0.7920.34
94_L228_L0.7860.33
34_G167_F0.7860.33
61_I214_G0.7840.33
68_V122_L0.7830.33
199_A202_I0.7800.33
89_N175_A0.7790.33
36_P40_I0.7770.33
224_I228_L0.7740.33
157_L171_A0.7700.32
117_G136_G0.7620.32
202_I231_W0.7570.32
87_F200_D0.7570.32
37_K154_F0.7550.32
72_L77_A0.7540.32
76_I202_I0.7470.31
54_G117_G0.7450.31
21_L193_A0.7450.31
190_A201_Q0.7440.31
35_P154_F0.7420.31
121_N144_I0.7390.31
41_I124_I0.7380.31
97_L184_M0.7370.31
15_P159_S0.7360.30
142_V176_S0.7350.30
66_T124_I0.7280.30
209_S212_L0.7270.30
70_P149_F0.7270.30
68_V136_G0.7250.30
18_I39_K0.7220.30
125_I140_L0.7220.30
73_P160_F0.7200.30
38_L70_P0.7190.29
142_V191_L0.7180.29
62_A149_F0.7150.29
129_Y147_T0.7140.29
213_T225_A0.7130.29
24_L29_I0.7130.29
62_A163_S0.7120.29
90_L228_L0.7100.29
28_I199_A0.7070.29
84_L129_Y0.7060.29
209_S224_I0.7060.29
147_T180_S0.7050.29
4_E147_T0.7010.28
47_K94_L0.7000.28
53_L68_V0.7000.28
123_A157_L0.6980.28
116_I178_V0.6960.28
91_G137_R0.6950.28
115_S217_G0.6940.28
27_L53_L0.6910.28
121_N125_I0.6820.27
37_K151_T0.6740.27
63_K167_F0.6690.27
174_M185_A0.6660.26
148_V231_W0.6640.26
90_L100_A0.6630.26
171_A225_A0.6630.26
167_F188_S0.6600.26
59_L159_S0.6470.25
113_T149_F0.6420.25
162_A178_V0.6420.25
136_G139_V0.6400.25
124_I222_L0.6400.25
169_P198_M0.6350.25
20_L204_A0.6340.25
75_I144_I0.6330.25
65_G105_L0.6320.25
65_G184_M0.6320.25
28_I72_L0.6320.25
5_L160_F0.6310.25
76_I80_P0.6300.25
184_M206_A0.6290.25
211_L216_T0.6290.25
110_I232_L0.6290.25
173_A204_A0.6290.25
148_V210_N0.6280.24
113_T141_G0.6270.24
160_F224_I0.6250.24
28_I231_W0.6220.24
108_E121_N0.6220.24
4_E24_L0.6200.24
69_G174_M0.6200.24
18_I159_S0.6180.24
149_F184_M0.6140.24
2_I12_P0.6130.24
2_I228_L0.6120.24
67_L192_A0.6120.24
124_I191_L0.6120.24
180_S186_A0.6120.24
125_I208_A0.6070.23
61_I64_L0.6000.23
109_A223_F0.5990.23
220_M228_L0.5980.23
55_I213_T0.5980.23
13_L156_L0.5970.23
27_L140_L0.5970.23
19_V120_P0.5960.23
97_L122_L0.5940.23
11_I43_E0.5870.22
27_L145_T0.5860.22
44_K76_I0.5850.22
133_S182_S0.5840.22
106_K166_I0.5820.22
122_L140_L0.5820.22
2_I44_K0.5820.22
24_L186_A0.5810.22
28_I46_M0.5810.22
109_A165_G0.5800.22
32_L75_I0.5780.22
32_L93_I0.5770.22
122_L213_T0.5770.22
153_F220_M0.5770.22
189_G226_L0.5690.22
56_A166_I0.5690.22
149_F231_W0.5670.21
3_A184_M0.5660.21
53_L155_G0.5660.21
11_I55_I0.5660.21
38_L65_G0.5640.21
215_V218_L0.5620.21
50_S176_S0.5610.21
29_I32_L0.5600.21
51_P102_L0.5590.21
46_M54_G0.5550.21
15_P23_M0.5540.21
117_G123_A0.5540.21
191_L194_A0.5530.21
91_G228_L0.5520.21
136_G140_L0.5500.21
21_L53_L0.5470.21
12_P77_A0.5460.20
204_A207_A0.5430.20
25_Y40_I0.5410.20
89_N191_L0.5400.20
59_L151_T0.5400.20
127_E214_G0.5390.20
3_A181_G0.5390.20
46_M145_T0.5350.20
93_I228_L0.5350.20
183_M186_A0.5320.20
151_T159_S0.5310.20
67_L205_F0.5300.20
74_K129_Y0.5260.20
4_E136_G0.5250.20
67_L74_K0.5240.19
218_L229_T0.5210.19
123_A142_V0.5210.19
11_I102_L0.5210.19
49_A147_T0.5200.19
144_I167_F0.5200.19
148_V163_S0.5190.19
19_V188_S0.5160.19
16_G156_L0.5150.19
78_A208_A0.5150.19
147_T182_S0.5120.19
17_T129_Y0.5110.19
1_V5_L0.5110.19
106_K122_L0.5110.19
71_N211_L0.5100.19
148_V185_A0.5090.19
28_I94_L0.5090.19
11_I17_T0.5090.19
34_G60_L0.5070.19
68_V104_G0.5070.19
104_G108_E0.5050.19
27_L201_Q0.5020.19
43_E59_L0.5000.18
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4m64A 1 0.6979 51.1 0.963 Contact Map
4ldsA 2 0.566 36.1 0.966 Contact Map
4q65A 1 0.6468 32.7 0.967 Contact Map
2gfpA 2 0.6213 29.6 0.967 Contact Map
4pypA 1 0.6638 22.9 0.969 Contact Map
4gc0A 1 0.6085 16.9 0.971 Contact Map
4ikvA 1 0.7021 16.7 0.971 Contact Map
4bemJ 1 0.6851 15.9 0.971 Contact Map
1pw4A 1 0.7106 15.8 0.971 Contact Map
4tphA 2 0.6638 15.2 0.972 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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