GREMLIN Database
DUF3025 - Protein of unknown function (DUF3025)
PFAM: PF11227 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 213 (211)
Sequences: 2380 (1659)
Seq/√Len: 114.2
META: 0.855

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
101_D104_E4.3751.00
84_A139_A3.7031.00
109_R112_A3.2631.00
194_W202_F3.1951.00
103_K204_D3.1701.00
59_E62_R2.7491.00
79_F122_F2.6271.00
100_H212_G2.6111.00
18_A22_E2.5861.00
128_E131_L2.5471.00
95_A99_A2.5231.00
14_L22_E2.4291.00
94_L143_L2.2371.00
104_E108_E2.2131.00
118_R158_Q2.1681.00
87_A162_L2.1661.00
126_L139_A2.1521.00
113_W117_A2.1421.00
135_K205_D2.0681.00
45_F120_V2.0451.00
150_F158_Q1.9821.00
51_A55_L1.9501.00
33_W131_L1.9391.00
48_T166_L1.9151.00
41_V127_L1.8761.00
88_S94_L1.8531.00
110_R197_N1.8271.00
103_K107_W1.8181.00
52_L122_F1.8121.00
205_D208_V1.7691.00
84_A119_F1.7591.00
59_E72_L1.7411.00
84_A190_V1.6791.00
48_T122_F1.6781.00
82_N187_V1.6391.00
107_W200_P1.6271.00
51_A54_A1.6051.00
129_K137_I1.5901.00
119_F190_V1.5741.00
104_E109_R1.5631.00
100_H184_P1.5161.00
55_L72_L1.4971.00
54_A58_A1.4851.00
188_L194_W1.4681.00
203_Y209_F1.4471.00
92_A109_R1.4351.00
82_N125_A1.4281.00
135_K208_V1.4241.00
82_N140_K1.4131.00
19_R23_T1.3991.00
103_K108_E1.3981.00
90_D116_R1.3851.00
197_N200_P1.3481.00
202_F208_V1.3461.00
141_A185_L1.3251.00
79_F85_I1.3181.00
55_L76_L1.3151.00
85_I166_L1.3151.00
26_V39_A1.3121.00
41_V44_A1.2970.99
19_R27_P1.2910.99
120_V158_Q1.2840.99
112_A116_R1.2700.99
4_V25_E1.2580.99
59_E70_G1.2570.99
86_V141_A1.2460.99
107_W110_R1.2410.99
91_P145_P1.2410.99
14_L19_R1.2390.99
19_R25_E1.2250.99
84_A141_A1.2190.99
96_L105_L1.2050.99
103_K200_P1.2000.99
86_V119_F1.1990.99
154_P157_A1.1980.99
38_N74_D1.1970.99
2_R25_E1.1860.99
100_H183_A1.1740.99
71_P179_P1.1720.99
82_N129_K1.1720.99
155_L158_Q1.1610.99
196_A199_D1.1540.99
86_V117_A1.1490.99
135_K202_F1.1360.99
96_L109_R1.1230.99
52_L76_L1.1210.99
6_Q9_L1.1190.99
96_L104_E1.1190.99
87_A146_V1.1170.98
136_G208_V1.1060.98
15_A18_A1.1030.98
97_L143_L1.1000.98
14_L23_T1.0880.98
70_G74_D1.0870.98
47_R164_A1.0800.98
113_W190_V1.0790.98
90_D112_A1.0770.98
70_G73_R1.0660.98
151_F158_Q1.0660.98
89_A116_R1.0620.98
111_A114_G1.0580.98
88_S143_L1.0550.98
100_H211_P1.0340.97
196_A200_P1.0300.97
8_A25_E1.0260.97
188_L208_V1.0240.97
14_L18_A1.0180.97
200_P204_D1.0140.97
39_A43_L1.0140.97
82_N126_L1.0080.97
142_L169_A0.9980.97
16_Y20_I0.9940.97
150_F153_L0.9830.97
134_Y140_K0.9690.96
51_A167_A0.9670.96
133_P137_I0.9660.96
96_L117_A0.9590.96
45_F122_F0.9570.96
82_N137_I0.9400.96
60_I64_G0.9280.95
93_L105_L0.9260.95
56_H73_R0.9210.95
98_R183_A0.9200.95
194_W197_N0.9180.95
200_P206_A0.9140.95
36_L182_L0.9120.95
95_A98_R0.9100.95
146_V161_A0.9080.95
63_E73_R0.9060.95
4_V19_R0.8960.94
179_P182_L0.8920.94
83_G137_I0.8900.94
199_D206_A0.8870.94
56_H60_I0.8850.94
83_G142_L0.8810.94
45_F85_I0.8780.94
6_Q10_P0.8780.94
113_W119_F0.8770.94
83_G122_F0.8760.94
206_A211_P0.8720.93
41_V45_F0.8720.93
121_V137_I0.8690.93
155_L159_L0.8660.93
59_E63_E0.8650.93
79_F83_G0.8640.93
126_L129_K0.8610.93
185_L190_V0.8550.93
96_L101_D0.8550.93
89_A94_L0.8500.93
128_E138_T0.8490.93
48_T51_A0.8480.92
33_W128_E0.8450.92
169_A176_L0.8360.92
165_A169_A0.8350.92
142_L176_L0.8340.92
194_W198_E0.8270.92
28_T39_A0.8260.91
32_N128_E0.8230.91
87_A120_V0.8200.91
15_A19_R0.8170.91
163_D167_A0.8040.90
129_K134_Y0.8040.90
74_D180_R0.8030.90
195_P198_E0.8000.90
37_F131_L0.7980.90
17_E53_N0.7970.90
75_A179_P0.7940.90
32_N36_L0.7810.89
84_A121_V0.7730.89
205_D210_R0.7670.88
44_A159_L0.7660.88
55_L181_P0.7630.88
60_I63_E0.7620.88
130_L137_I0.7590.88
198_E203_Y0.7580.88
19_R24_G0.7560.87
121_V127_L0.7510.87
28_T32_N0.7510.87
141_A184_P0.7500.87
193_W202_F0.7460.87
156_A159_L0.7450.87
20_I52_L0.7380.86
201_A204_D0.7300.86
93_L106_F0.7290.85
105_L117_A0.7280.85
6_Q73_R0.7260.85
24_G50_A0.7260.85
85_I142_L0.7180.85
21_F57_A0.7070.84
91_P95_A0.7060.84
21_F27_P0.7050.84
133_P188_L0.7030.83
142_L166_L0.7010.83
118_R151_F0.7000.83
16_Y28_T0.6990.83
65_V68_R0.6990.83
147_P161_A0.6980.83
60_I125_A0.6980.83
146_V158_Q0.6950.83
5_P8_A0.6950.83
126_L137_I0.6910.82
18_A27_P0.6900.82
20_I27_P0.6880.82
87_A150_F0.6870.82
53_N56_H0.6870.82
119_F139_A0.6860.82
138_T193_W0.6850.82
97_L100_H0.6800.81
89_A145_P0.6770.81
120_V162_L0.6770.81
3_F26_V0.6760.81
71_P75_A0.6650.80
190_V194_W0.6650.80
73_R77_T0.6630.80
76_L125_A0.6630.80
99_A147_P0.6610.79
105_L183_A0.6600.79
41_V56_H0.6590.79
45_F48_T0.6570.79
89_A149_D0.6570.79
33_W176_L0.6510.78
79_F125_A0.6510.78
150_F155_L0.6500.78
135_K188_L0.6490.78
47_R161_A0.6470.78
199_D204_D0.6460.78
48_T163_D0.6460.78
44_A127_L0.6450.78
170_L177_L0.6450.78
77_T102_W0.6440.78
79_F82_N0.6370.77
19_R22_E0.6370.77
93_L117_A0.6350.77
176_L182_L0.6340.77
20_I139_A0.6310.76
127_L208_V0.6310.76
190_V203_Y0.6300.76
129_K140_K0.6270.76
78_L140_K0.6240.75
197_N202_F0.6240.75
22_E57_A0.6220.75
161_A165_A0.6210.75
139_A208_V0.6180.75
16_Y38_N0.6180.75
75_A176_L0.6170.75
143_L183_A0.6140.74
5_P30_P0.6120.74
203_Y210_R0.6110.74
100_H105_L0.6100.74
153_L161_A0.6090.74
134_Y138_T0.6080.74
107_W199_D0.6050.73
108_E111_A0.6000.73
56_H82_N0.6000.73
92_A95_A0.6000.73
177_L181_P0.5980.72
89_A146_V0.5970.72
129_K133_P0.5960.72
87_A118_R0.5960.72
45_F163_D0.5920.72
99_A212_G0.5910.71
170_L173_G0.5910.71
64_G67_A0.5860.71
153_L157_A0.5850.71
63_E66_G0.5840.71
169_A175_A0.5840.71
127_L135_K0.5770.70
57_A78_L0.5760.69
110_R113_W0.5750.69
38_N131_L0.5730.69
144_V166_L0.5710.69
22_E61_A0.5700.69
17_E73_R0.5670.68
152_A157_A0.5670.68
83_G121_V0.5660.68
8_A12_G0.5650.68
125_A137_I0.5630.68
150_F161_A0.5630.68
22_E27_P0.5630.68
142_L182_L0.5600.67
21_F67_A0.5590.67
196_A202_F0.5550.67
16_Y139_A0.5540.67
46_P163_D0.5530.66
163_D166_L0.5530.66
50_A54_A0.5520.66
47_R50_A0.5520.66
121_V126_L0.5470.66
52_L83_G0.5460.65
21_F53_N0.5430.65
33_W36_L0.5360.64
47_R163_D0.5350.64
72_L173_G0.5340.64
193_W208_V0.5310.63
72_L142_L0.5290.63
141_A181_P0.5290.63
120_V150_F0.5280.63
23_T27_P0.5260.63
154_P158_Q0.5260.63
119_F140_K0.5240.62
89_A151_F0.5230.62
122_F188_L0.5210.62
64_G68_R0.5210.62
38_N73_R0.5180.61
48_T85_I0.5140.61
85_I163_D0.5120.60
88_S119_F0.5090.60
96_L107_W0.5080.60
28_T31_D0.5070.60
91_P169_A0.5060.60
73_R140_K0.5060.60
161_A164_A0.5040.59
15_A73_R0.5020.59
73_R138_T0.5000.59
87_A143_L0.5000.59
88_S93_L0.5000.59
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3k9vA 2 0.9108 16.1 0.966 Contact Map
4l54A 1 0.7981 13.4 0.967 Contact Map
2xbkA 1 0.77 10.6 0.969 Contact Map
2rfbA 1 0.7559 10.1 0.969 Contact Map
3nc3A 1 0.7418 10.1 0.969 Contact Map
1izoA 1 0.8122 9.6 0.969 Contact Map
3b6hA 1 0.9061 9.6 0.969 Contact Map
4oqrA 1 0.7793 8.9 0.97 Contact Map
3czhA 2 0.892 8.8 0.97 Contact Map
4l0fA 1 0.784 8.3 0.97 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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