DUF3025 - PFAM28P - GREMLIN Contacts

Home
GREMLIN
- Submit - Monomer
- Submit - Complex
Contacts
Metagenomics
- Contacts
- Structures
Structures
- List
Old Projects
- Complexes (2014)
- PFAM (2013)

DUF3025 - Protein of unknown function (DUF3025)

PFAM:	PF11227	Sequences: LRFVPQDALPAGDLAYEARIFETGEVPTRPDNWHDLFNALVWLAFPRTKAALNALHAAEIAREGVGARRGPLRDALTLFDENGAIVASADPALLALLRAHDWKELFWERRAAWGRRARFVVFGHALLEKLLAPYKGITAKALLVPVPPDFFALPLAAQLAALDAALAAALAAGAALLSPRPLAPLPVLGVPGWWPANEDPAFYDDARVFRPGR	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_10
Length:	213 (211)
Sequences:	2380 (1659)
Seq/√Len:	114.2
META:	0.855

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
101_D	104_E	4.375	1.00
84_A	139_A	3.703	1.00
109_R	112_A	3.263	1.00
194_W	202_F	3.195	1.00
103_K	204_D	3.170	1.00
59_E	62_R	2.749	1.00
79_F	122_F	2.627	1.00
100_H	212_G	2.611	1.00
18_A	22_E	2.586	1.00
128_E	131_L	2.547	1.00
95_A	99_A	2.523	1.00
14_L	22_E	2.429	1.00
94_L	143_L	2.237	1.00
104_E	108_E	2.213	1.00
118_R	158_Q	2.168	1.00
87_A	162_L	2.166	1.00
126_L	139_A	2.152	1.00
113_W	117_A	2.142	1.00
135_K	205_D	2.068	1.00
45_F	120_V	2.045	1.00
150_F	158_Q	1.982	1.00
51_A	55_L	1.950	1.00
33_W	131_L	1.939	1.00
48_T	166_L	1.915	1.00
41_V	127_L	1.876	1.00
88_S	94_L	1.853	1.00
110_R	197_N	1.827	1.00
103_K	107_W	1.818	1.00
52_L	122_F	1.812	1.00
205_D	208_V	1.769	1.00
84_A	119_F	1.759	1.00
59_E	72_L	1.741	1.00
84_A	190_V	1.679	1.00
48_T	122_F	1.678	1.00
82_N	187_V	1.639	1.00
107_W	200_P	1.627	1.00
51_A	54_A	1.605	1.00
129_K	137_I	1.590	1.00
119_F	190_V	1.574	1.00
104_E	109_R	1.563	1.00
100_H	184_P	1.516	1.00
55_L	72_L	1.497	1.00
54_A	58_A	1.485	1.00
188_L	194_W	1.468	1.00
203_Y	209_F	1.447	1.00
92_A	109_R	1.435	1.00
82_N	125_A	1.428	1.00
135_K	208_V	1.424	1.00
82_N	140_K	1.413	1.00
19_R	23_T	1.399	1.00
103_K	108_E	1.398	1.00
90_D	116_R	1.385	1.00
197_N	200_P	1.348	1.00
202_F	208_V	1.346	1.00
141_A	185_L	1.325	1.00
79_F	85_I	1.318	1.00
55_L	76_L	1.315	1.00
85_I	166_L	1.315	1.00
26_V	39_A	1.312	1.00
41_V	44_A	1.297	0.99
19_R	27_P	1.291	0.99
120_V	158_Q	1.284	0.99
112_A	116_R	1.270	0.99
4_V	25_E	1.258	0.99
59_E	70_G	1.257	0.99
86_V	141_A	1.246	0.99
107_W	110_R	1.241	0.99
91_P	145_P	1.241	0.99
14_L	19_R	1.239	0.99
19_R	25_E	1.225	0.99
84_A	141_A	1.219	0.99
96_L	105_L	1.205	0.99
103_K	200_P	1.200	0.99
86_V	119_F	1.199	0.99
154_P	157_A	1.198	0.99
38_N	74_D	1.197	0.99
2_R	25_E	1.186	0.99
100_H	183_A	1.174	0.99
71_P	179_P	1.172	0.99
82_N	129_K	1.172	0.99
155_L	158_Q	1.161	0.99
196_A	199_D	1.154	0.99
86_V	117_A	1.149	0.99
135_K	202_F	1.136	0.99
96_L	109_R	1.123	0.99
52_L	76_L	1.121	0.99
6_Q	9_L	1.119	0.99
96_L	104_E	1.119	0.99
87_A	146_V	1.117	0.98
136_G	208_V	1.106	0.98
15_A	18_A	1.103	0.98
97_L	143_L	1.100	0.98
14_L	23_T	1.088	0.98
70_G	74_D	1.087	0.98
47_R	164_A	1.080	0.98
113_W	190_V	1.079	0.98
90_D	112_A	1.077	0.98
70_G	73_R	1.066	0.98
151_F	158_Q	1.066	0.98
89_A	116_R	1.062	0.98
111_A	114_G	1.058	0.98
88_S	143_L	1.055	0.98
100_H	211_P	1.034	0.97
196_A	200_P	1.030	0.97
8_A	25_E	1.026	0.97
188_L	208_V	1.024	0.97
14_L	18_A	1.018	0.97
200_P	204_D	1.014	0.97
39_A	43_L	1.014	0.97
82_N	126_L	1.008	0.97
142_L	169_A	0.998	0.97
16_Y	20_I	0.994	0.97
150_F	153_L	0.983	0.97
134_Y	140_K	0.969	0.96
51_A	167_A	0.967	0.96
133_P	137_I	0.966	0.96
96_L	117_A	0.959	0.96
45_F	122_F	0.957	0.96
82_N	137_I	0.940	0.96
60_I	64_G	0.928	0.95
93_L	105_L	0.926	0.95
56_H	73_R	0.921	0.95
98_R	183_A	0.920	0.95
194_W	197_N	0.918	0.95
200_P	206_A	0.914	0.95
36_L	182_L	0.912	0.95
95_A	98_R	0.910	0.95
146_V	161_A	0.908	0.95
63_E	73_R	0.906	0.95
4_V	19_R	0.896	0.94
179_P	182_L	0.892	0.94
83_G	137_I	0.890	0.94
199_D	206_A	0.887	0.94
56_H	60_I	0.885	0.94
83_G	142_L	0.881	0.94
45_F	85_I	0.878	0.94
6_Q	10_P	0.878	0.94
113_W	119_F	0.877	0.94
83_G	122_F	0.876	0.94
206_A	211_P	0.872	0.93
41_V	45_F	0.872	0.93
121_V	137_I	0.869	0.93
155_L	159_L	0.866	0.93
59_E	63_E	0.865	0.93
79_F	83_G	0.864	0.93
126_L	129_K	0.861	0.93
185_L	190_V	0.855	0.93
96_L	101_D	0.855	0.93
89_A	94_L	0.850	0.93
128_E	138_T	0.849	0.93
48_T	51_A	0.848	0.92
33_W	128_E	0.845	0.92
169_A	176_L	0.836	0.92
165_A	169_A	0.835	0.92
142_L	176_L	0.834	0.92
194_W	198_E	0.827	0.92
28_T	39_A	0.826	0.91
32_N	128_E	0.823	0.91
87_A	120_V	0.820	0.91
15_A	19_R	0.817	0.91
163_D	167_A	0.804	0.90
129_K	134_Y	0.804	0.90
74_D	180_R	0.803	0.90
195_P	198_E	0.800	0.90
37_F	131_L	0.798	0.90
17_E	53_N	0.797	0.90
75_A	179_P	0.794	0.90
32_N	36_L	0.781	0.89
84_A	121_V	0.773	0.89
205_D	210_R	0.767	0.88
44_A	159_L	0.766	0.88
55_L	181_P	0.763	0.88
60_I	63_E	0.762	0.88
130_L	137_I	0.759	0.88
198_E	203_Y	0.758	0.88
19_R	24_G	0.756	0.87
121_V	127_L	0.751	0.87
28_T	32_N	0.751	0.87
141_A	184_P	0.750	0.87
193_W	202_F	0.746	0.87
156_A	159_L	0.745	0.87
20_I	52_L	0.738	0.86
201_A	204_D	0.730	0.86
93_L	106_F	0.729	0.85
105_L	117_A	0.728	0.85
6_Q	73_R	0.726	0.85
24_G	50_A	0.726	0.85
85_I	142_L	0.718	0.85
21_F	57_A	0.707	0.84
91_P	95_A	0.706	0.84
21_F	27_P	0.705	0.84
133_P	188_L	0.703	0.83
142_L	166_L	0.701	0.83
118_R	151_F	0.700	0.83
16_Y	28_T	0.699	0.83
65_V	68_R	0.699	0.83
147_P	161_A	0.698	0.83
60_I	125_A	0.698	0.83
146_V	158_Q	0.695	0.83
5_P	8_A	0.695	0.83
126_L	137_I	0.691	0.82
18_A	27_P	0.690	0.82
20_I	27_P	0.688	0.82
87_A	150_F	0.687	0.82
53_N	56_H	0.687	0.82
119_F	139_A	0.686	0.82
138_T	193_W	0.685	0.82
97_L	100_H	0.680	0.81
89_A	145_P	0.677	0.81
120_V	162_L	0.677	0.81
3_F	26_V	0.676	0.81
71_P	75_A	0.665	0.80
190_V	194_W	0.665	0.80
73_R	77_T	0.663	0.80
76_L	125_A	0.663	0.80
99_A	147_P	0.661	0.79
105_L	183_A	0.660	0.79
41_V	56_H	0.659	0.79
45_F	48_T	0.657	0.79
89_A	149_D	0.657	0.79
33_W	176_L	0.651	0.78
79_F	125_A	0.651	0.78
150_F	155_L	0.650	0.78
135_K	188_L	0.649	0.78
47_R	161_A	0.647	0.78
199_D	204_D	0.646	0.78
48_T	163_D	0.646	0.78
44_A	127_L	0.645	0.78
170_L	177_L	0.645	0.78
77_T	102_W	0.644	0.78
79_F	82_N	0.637	0.77
19_R	22_E	0.637	0.77
93_L	117_A	0.635	0.77
176_L	182_L	0.634	0.77
20_I	139_A	0.631	0.76
127_L	208_V	0.631	0.76
190_V	203_Y	0.630	0.76
129_K	140_K	0.627	0.76
78_L	140_K	0.624	0.75
197_N	202_F	0.624	0.75
22_E	57_A	0.622	0.75
161_A	165_A	0.621	0.75
139_A	208_V	0.618	0.75
16_Y	38_N	0.618	0.75
75_A	176_L	0.617	0.75
143_L	183_A	0.614	0.74
5_P	30_P	0.612	0.74
203_Y	210_R	0.611	0.74
100_H	105_L	0.610	0.74
153_L	161_A	0.609	0.74
134_Y	138_T	0.608	0.74
107_W	199_D	0.605	0.73
108_E	111_A	0.600	0.73
56_H	82_N	0.600	0.73
92_A	95_A	0.600	0.73
177_L	181_P	0.598	0.72
89_A	146_V	0.597	0.72
129_K	133_P	0.596	0.72
87_A	118_R	0.596	0.72
45_F	163_D	0.592	0.72
99_A	212_G	0.591	0.71
170_L	173_G	0.591	0.71
64_G	67_A	0.586	0.71
153_L	157_A	0.585	0.71
63_E	66_G	0.584	0.71
169_A	175_A	0.584	0.71
127_L	135_K	0.577	0.70
57_A	78_L	0.576	0.69
110_R	113_W	0.575	0.69
38_N	131_L	0.573	0.69
144_V	166_L	0.571	0.69
22_E	61_A	0.570	0.69
17_E	73_R	0.567	0.68
152_A	157_A	0.567	0.68
83_G	121_V	0.566	0.68
8_A	12_G	0.565	0.68
125_A	137_I	0.563	0.68
150_F	161_A	0.563	0.68
22_E	27_P	0.563	0.68
142_L	182_L	0.560	0.67
21_F	67_A	0.559	0.67
196_A	202_F	0.555	0.67
16_Y	139_A	0.554	0.67
46_P	163_D	0.553	0.66
163_D	166_L	0.553	0.66
50_A	54_A	0.552	0.66
47_R	50_A	0.552	0.66
121_V	126_L	0.547	0.66
52_L	83_G	0.546	0.65
21_F	53_N	0.543	0.65
33_W	36_L	0.536	0.64
47_R	163_D	0.535	0.64
72_L	173_G	0.534	0.64
193_W	208_V	0.531	0.63
72_L	142_L	0.529	0.63
141_A	181_P	0.529	0.63
120_V	150_F	0.528	0.63
23_T	27_P	0.526	0.63
154_P	158_Q	0.526	0.63
119_F	140_K	0.524	0.62
89_A	151_F	0.523	0.62
122_F	188_L	0.521	0.62
64_G	68_R	0.521	0.62
38_N	73_R	0.518	0.61
48_T	85_I	0.514	0.61
85_I	163_D	0.512	0.60
88_S	119_F	0.509	0.60
96_L	107_W	0.508	0.60
28_T	31_D	0.507	0.60
91_P	169_A	0.506	0.60
73_R	140_K	0.506	0.60
161_A	164_A	0.504	0.59
15_A	73_R	0.502	0.59
73_R	138_T	0.500	0.59
87_A	143_L	0.500	0.59
88_S	93_L	0.500	0.59

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3k9vA	2	0.9108	16.1	0.966	Contact Map
4l54A	1	0.7981	13.4	0.967	Contact Map
2xbkA	1	0.77	10.6	0.969	Contact Map
2rfbA	1	0.7559	10.1	0.969	Contact Map
3nc3A	1	0.7418	10.1	0.969	Contact Map
1izoA	1	0.8122	9.6	0.969	Contact Map
3b6hA	1	0.9061	9.6	0.969	Contact Map
4oqrA	1	0.7793	8.9	0.97	Contact Map
3czhA	2	0.892	8.8	0.97	Contact Map
4l0fA	1	0.784	8.3	0.97	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0083 seconds.