GREMLIN Database
DUF2854 - Protein of unknown function (DUF2854)
PFAM: PF11016 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 146 (145)
Sequences: 502 (303)
Seq/√Len: 25.1
META: 0.685

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
123_L145_V3.5911.00
81_S85_A3.4151.00
18_F22_I3.3871.00
68_V98_L2.9751.00
106_T111_A2.8091.00
3_F7_F2.6270.99
63_Q136_F2.6110.99
62_N65_R2.4180.98
51_A55_Q2.3050.98
32_K35_E2.2880.98
133_L141_V2.1360.97
133_L143_A2.1050.96
72_R79_L2.0700.96
42_S100_G2.0660.96
102_R115_E1.9660.94
49_V105_E1.7680.91
76_E80_D1.6760.88
104_E111_A1.6710.88
68_V72_R1.6570.87
90_P95_R1.6540.87
42_S115_E1.6480.87
103_E110_Y1.6420.87
121_V129_K1.6320.87
99_T117_D1.6190.86
8_T16_P1.5680.84
76_E133_L1.5570.84
65_R112_L1.5210.82
12_T15_L1.5190.82
12_T16_P1.5130.82
37_K76_E1.4950.81
102_R113_V1.4760.80
122_P135_R1.4490.79
131_E135_R1.4240.78
72_R136_F1.4160.78
76_E89_S1.4090.77
130_Q134_E1.3930.76
100_G115_E1.3910.76
97_Q121_V1.3740.75
10_N15_L1.3520.74
83_L116_F1.3290.73
4_V34_A1.3290.73
104_E113_V1.3200.72
56_Q114_L1.3170.72
53_R103_E1.3120.72
124_E128_E1.3110.72
11_A15_L1.2940.71
127_Q130_Q1.2920.71
64_I121_V1.2700.69
103_E112_L1.2610.69
95_R121_V1.2450.68
15_L19_F1.1910.64
63_Q72_R1.1890.64
48_E105_E1.1870.64
29_L88_L1.1690.63
9_G18_F1.1640.62
3_F9_G1.1540.62
89_S95_R1.1510.62
85_A143_A1.1460.61
53_R110_Y1.1310.60
7_F11_A1.1260.60
59_P136_F1.1200.59
53_R112_L1.1200.59
91_T121_V1.1190.59
27_G30_A1.1090.59
34_A92_D1.1090.59
128_E143_A1.0990.58
22_I25_L1.0890.57
68_V101_I1.0870.57
64_I141_V1.0820.57
34_A76_E1.0810.57
141_V145_V1.0770.56
58_T62_N1.0750.56
12_T17_G1.0660.56
15_L33_A1.0580.55
82_S133_L1.0520.55
98_L133_L1.0470.54
84_E108_G1.0240.53
13_L19_F1.0130.52
79_L136_F1.0130.52
24_I32_K1.0080.51
8_T11_A1.0000.51
86_L129_K0.9930.50
132_K143_A0.9840.50
61_Q111_A0.9730.49
36_L75_Q0.9650.48
34_A72_R0.9570.48
2_G5_A0.9530.47
65_R103_E0.9410.47
20_Y28_G0.9370.46
58_T138_G0.9340.46
117_D124_E0.9310.46
80_D140_G0.9240.45
6_Y35_E0.9130.45
91_T120_L0.9130.45
23_P26_L0.9020.44
82_S85_A0.8790.42
18_F122_P0.8710.42
48_E69_T0.8700.41
24_I84_E0.8690.41
55_Q99_T0.8690.41
52_L105_E0.8680.41
4_V8_T0.8580.41
85_A133_L0.8540.40
36_L110_Y0.8490.40
61_Q83_L0.8430.40
56_Q109_A0.8420.40
39_V77_A0.8350.39
7_F27_G0.8340.39
33_A75_Q0.8230.38
82_S134_E0.8190.38
81_S136_F0.8170.38
17_G24_I0.8110.37
59_P72_R0.8000.37
60_T139_P0.7890.36
80_D87_G0.7800.35
72_R82_S0.7790.35
63_Q85_A0.7700.35
98_L114_L0.7680.34
13_L17_G0.7660.34
64_I143_A0.7650.34
1_I16_P0.7630.34
82_S93_E0.7580.34
134_E143_A0.7560.34
41_F45_T0.7550.34
4_V7_F0.7540.34
101_I134_E0.7490.33
99_T144_E0.7480.33
1_I145_V0.7460.33
30_A78_H0.7440.33
47_P54_E0.7390.33
52_L139_P0.7320.32
27_G32_K0.7290.32
2_G6_Y0.7220.32
64_I97_Q0.7200.31
11_A16_P0.7200.31
5_A9_G0.7190.31
70_R75_Q0.7160.31
125_R128_E0.7030.30
61_Q112_L0.6960.30
29_L36_L0.6870.29
49_V60_T0.6840.29
128_E133_L0.6810.29
7_F18_F0.6770.29
134_E142_R0.6740.29
4_V27_G0.6730.29
89_S121_V0.6680.28
130_Q138_G0.6650.28
48_E97_Q0.6590.28
51_A101_I0.6560.28
27_G35_E0.6560.28
1_I45_T0.6470.27
77_A97_Q0.6440.27
40_P58_T0.6400.27
84_E135_R0.6390.27
59_P114_L0.6370.26
39_V69_T0.6370.26
80_D95_R0.6310.26
6_Y32_K0.6300.26
52_L138_G0.6270.26
40_P112_L0.6240.26
21_G26_L0.6220.26
57_A62_N0.6210.26
72_R98_L0.6170.25
3_F18_F0.6140.25
100_G113_V0.6130.25
17_G118_S0.6090.25
125_R145_V0.6090.25
59_P66_K0.6000.24
68_V79_L0.5980.24
47_P134_E0.5980.24
26_L69_T0.5940.24
118_S126_W0.5930.24
84_E106_T0.5910.24
58_T139_P0.5870.24
117_D145_V0.5870.24
87_G95_R0.5840.24
44_P129_K0.5840.24
48_E130_Q0.5830.24
15_L84_E0.5830.24
34_A136_F0.5820.23
36_L78_H0.5760.23
88_L126_W0.5740.23
134_E141_V0.5730.23
46_S64_I0.5720.23
4_V10_N0.5720.23
74_G80_D0.5720.23
5_A103_E0.5700.23
51_A122_P0.5660.23
29_L132_K0.5630.22
93_E121_V0.5610.22
1_I22_I0.5600.22
46_S76_E0.5560.22
30_A90_P0.5560.22
13_L16_P0.5550.22
30_A36_L0.5530.22
39_V106_T0.5500.22
60_T100_G0.5500.22
25_L92_D0.5440.22
20_Y31_L0.5380.21
21_G24_I0.5350.21
18_F86_L0.5350.21
15_L32_K0.5310.21
4_V49_V0.5300.21
8_T24_I0.5300.21
30_A92_D0.5300.21
61_Q126_W0.5260.21
44_P100_G0.5240.21
24_I44_P0.5220.20
12_T19_F0.5190.20
118_S123_L0.5190.20
77_A135_R0.5180.20
104_E109_A0.5160.20
39_V99_T0.5150.20
98_L143_A0.5120.20
95_R113_V0.5110.20
61_Q110_Y0.5100.20
58_T114_L0.5030.20
56_Q100_G0.5000.19
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3tqmA 1 0.2397 19.1 0.954 Contact Map
3v26X 1 0.2397 19 0.954 Contact Map
2ywqA 1 0.2397 18.1 0.955 Contact Map
4heiA 1 0.2397 13.6 0.957 Contact Map
2l60A 1 0.2671 11.6 0.958 Contact Map
1f8pA 1 0.2466 8.2 0.961 Contact Map
1qbfA 1 0.2466 8 0.961 Contact Map
1xatA 3 0.6301 7.3 0.962 Contact Map
4k0jA 3 0.8288 6.2 0.963 Contact Map
3v2cY 1 0.2671 5.7 0.964 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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