GREMLIN Database
Holin_BhlA - BhlA holin family
PFAM: PF10960 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 70 (69)
Sequences: 656 (528)
Seq/√Len: 63.5
META: 0.668

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
4_E7_K4.2621.00
9_A14_I3.0031.00
64_D69_K2.8671.00
41_E44_Y2.4611.00
40_R44_Y2.3781.00
9_A20_V2.3451.00
19_F23_L2.2091.00
10_L17_V2.1081.00
43_K46_E1.6930.99
50_K53_E1.6120.99
15_W19_F1.6090.99
37_Q41_E1.5330.99
34_E37_Q1.5180.99
23_L30_N1.5040.98
55_L58_V1.4410.98
54_K57_I1.4410.98
37_Q40_R1.3550.97
19_F26_V1.2620.95
51_L54_K1.2250.95
30_N34_E1.1970.94
46_E49_D1.1920.94
23_L26_V1.1870.94
62_K66_E1.1690.93
26_V30_N1.1510.93
12_Q29_E1.1470.92
44_Y47_I1.1160.91
63_K66_E1.0880.90
43_K49_D1.0820.90
46_E50_K1.0740.90
12_Q24_F1.0680.89
64_D67_E1.0620.89
36_K39_E1.0540.89
44_Y51_L1.0420.88
33_R37_Q1.0350.88
3_E6_L0.9850.85
51_L58_V0.9650.84
2_E12_Q0.9650.84
25_Y32_K0.9610.84
47_I51_L0.9520.83
42_E46_E0.9380.82
21_W24_F0.9340.82
61_I65_V0.9240.81
38_E44_Y0.9200.81
52_T55_L0.9140.81
60_D63_K0.9110.80
45_Q48_I0.8780.78
21_W25_Y0.8700.77
44_Y48_I0.8670.77
58_V61_I0.8480.75
15_W21_W0.8450.75
51_L55_L0.8320.74
38_E41_E0.8080.72
23_L34_E0.7970.71
35_E39_E0.7950.71
5_I24_F0.7930.70
19_F34_E0.7930.70
21_W27_L0.7610.67
33_R36_K0.7440.66
34_E38_E0.7390.65
44_Y55_L0.7390.65
8_L11_S0.7390.65
44_Y52_T0.7350.65
64_D68_I0.7350.65
27_L30_N0.7350.65
54_K59_E0.7340.64
33_R40_R0.7080.62
33_R41_E0.7060.61
4_E11_S0.6980.61
60_D68_I0.6950.60
13_G23_L0.6940.60
35_E38_E0.6640.57
9_A15_W0.6620.57
5_I46_E0.6610.56
58_V64_D0.6540.56
34_E41_E0.6530.56
49_D66_E0.6460.55
14_I22_L0.6390.54
19_F37_Q0.6330.53
48_I51_L0.6290.53
19_F30_N0.6280.53
40_R45_Q0.6260.52
51_L61_I0.6120.51
5_I56_E0.5950.49
48_I55_L0.5900.48
41_E45_Q0.5840.48
11_S52_T0.5830.47
37_Q44_Y0.5780.47
16_A30_N0.5770.47
5_I9_A0.5740.46
35_E53_E0.5710.46
32_K35_E0.5670.46
56_E63_K0.5620.45
34_E44_Y0.5560.44
4_E8_L0.5540.44
9_A21_W0.5520.44
50_K56_E0.5480.43
24_F30_N0.5430.43
11_S28_K0.5430.43
9_A17_V0.5360.42
40_R48_I0.5100.39
8_L14_I0.5080.39
12_Q30_N0.5060.39
45_Q57_I0.5020.38
3_E7_K0.5000.38
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4cbcA 3 0.3857 42.6 0.833 Contact Map
4ltoA 3 1 28.7 0.847 Contact Map
2rddB 1 0.5286 21.5 0.857 Contact Map
3ziaG 1 1 15.9 0.865 Contact Map
2ksdA 1 0.5143 13.3 0.87 Contact Map
2qe7G 1 0.7 10.8 0.875 Contact Map
4pz1A 2 0.6286 10.7 0.875 Contact Map
2xokG 1 1 10.3 0.876 Contact Map
2ck3G 1 0.8429 10 0.876 Contact Map
4h5yA 1 0.9429 9 0.879 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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