GREMLIN Database
DUF2516 - Protein of unknown function (DUF2516)
PFAM: PF10724 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 92 (88)
Sequences: 3011 (2041)
Seq/√Len: 217.6
META: 0.79

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
39_L43_A4.2491.00
75_L79_V3.7581.00
24_D28_R3.2051.00
22_F49_G2.6671.00
47_I73_A2.6341.00
42_P46_A2.4711.00
18_E55_G2.3201.00
46_A50_V2.2171.00
25_A33_F2.1531.00
24_D77_Y2.1471.00
45_L49_G2.1081.00
5_L9_V1.8121.00
43_A47_I1.7991.00
82_R86_R1.7981.00
14_A71_V1.7921.00
26_L45_L1.7371.00
28_R33_F1.6521.00
30_A34_V1.6001.00
25_A45_L1.5701.00
8_L12_L1.5681.00
39_L47_I1.5671.00
19_V52_L1.5271.00
85_L88_V1.4961.00
17_V78_L1.4331.00
20_W78_L1.4251.00
85_L89_Q1.3541.00
25_A48_L1.3351.00
18_E74_A1.3261.00
65_L69_I1.3091.00
16_V20_W1.2681.00
71_V75_L1.1771.00
43_A46_A1.1371.00
28_R77_Y1.1351.00
18_E71_V1.1051.00
4_G8_L1.1041.00
19_V23_V1.0431.00
81_V85_L1.0361.00
22_F48_L0.9861.00
38_K84_A0.9851.00
13_A16_V0.9831.00
22_F26_L0.9791.00
84_A87_E0.9721.00
72_V76_V0.9661.00
51_A73_A0.9661.00
64_G67_G0.9591.00
86_R90_G0.9320.99
18_E67_G0.9280.99
54_L66_L0.8960.99
62_P67_G0.8910.99
29_P32_A0.8700.99
13_A17_V0.8640.99
14_A18_E0.8590.99
48_L52_L0.8580.99
53_L57_L0.8380.99
55_G71_V0.8240.99
55_G70_A0.8230.99
18_E70_A0.8210.99
5_L8_L0.8190.99
7_L11_G0.7980.98
74_A78_L0.7920.98
30_A42_P0.7850.98
54_L70_A0.7830.98
68_L71_V0.7780.98
20_W82_R0.7720.98
28_R89_Q0.7600.98
3_Q7_L0.7550.98
17_V71_V0.7500.98
51_A74_A0.7480.98
17_V75_L0.7450.98
60_G64_G0.7360.97
87_E90_G0.7300.97
76_V84_A0.7280.97
38_K83_P0.7240.97
15_L52_L0.7220.97
3_Q6_V0.7220.97
21_A78_L0.7160.97
8_L11_G0.7150.97
38_K80_D0.7140.97
33_F77_Y0.7140.97
33_F85_L0.7130.97
23_V27_R0.6970.96
38_K81_V0.6870.96
48_L69_I0.6790.96
47_I50_V0.6670.95
17_V74_A0.6540.95
9_V13_A0.6520.95
50_V54_L0.6490.95
15_L19_V0.6460.94
20_W24_D0.6340.94
55_G74_A0.6340.94
32_A85_L0.6110.93
22_F45_L0.6090.93
34_V37_G0.6060.92
63_L67_G0.5990.92
61_G64_G0.5960.92
67_G79_V0.5920.91
86_R89_Q0.5890.91
64_G68_L0.5790.91
11_G68_L0.5780.90
62_P68_L0.5740.90
68_L72_V0.5720.90
46_A49_G0.5720.90
4_G59_F0.5670.90
49_G56_L0.5650.89
80_D84_A0.5600.89
15_L18_E0.5590.89
24_D82_R0.5530.88
80_D83_P0.5510.88
52_L56_L0.5480.88
22_F52_L0.5450.88
21_A48_L0.5420.87
31_D88_V0.5360.87
78_L82_R0.5360.87
2_V5_L0.5340.87
62_P65_L0.5300.86
27_R82_R0.5280.86
54_L60_G0.5270.86
35_A88_V0.5250.86
81_V84_A0.5230.86
18_E52_L0.5230.86
51_A75_L0.5220.85
34_V40_T0.5160.85
61_G67_G0.5150.85
51_A70_A0.5130.84
33_F38_K0.5100.84
43_A84_A0.5090.84
48_L74_A0.5090.84
48_L77_Y0.5070.84
76_V83_P0.5060.84
59_F62_P0.5050.84
77_Y82_R0.5020.83
60_G67_G0.5000.83
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2rddB 1 0.3478 9 0.896 Contact Map
3wajA 1 0.9348 8 0.899 Contact Map
3rceA 1 0.8696 7.3 0.9 Contact Map
4qndA 2 0.4022 3.7 0.914 Contact Map
3j2wE 1 0.5 3.4 0.915 Contact Map
4mndA 2 0.837 3.4 0.915 Contact Map
2f93B 2 0.5543 3.3 0.916 Contact Map
2j6pA 4 0.3478 3 0.917 Contact Map
3ayfA 2 0.4674 2.3 0.922 Contact Map
3ixzA 1 1 2.1 0.924 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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