GREMLIN Database
DUF2514 - Protein of unknown function (DUF2514)
PFAM: PF10721 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 161 (155)
Sequences: 924 (684)
Seq/√Len: 54.9
META: 0.563

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
54_E57_R4.8291.00
132_D136_G4.1331.00
52_R55_E3.9311.00
132_D135_A3.8171.00
131_A135_A3.7531.00
141_A145_A3.2811.00
54_E58_R2.6381.00
134_R138_L2.5011.00
131_A134_R2.3951.00
151_A155_A2.3681.00
47_A51_A2.3191.00
140_A144_R2.3021.00
141_A146_R2.2611.00
133_E137_E1.9421.00
94_A97_L1.9371.00
64_E68_D1.9271.00
153_E157_D1.8770.99
65_A151_A1.8090.99
19_A22_R1.7660.99
30_Q33_W1.7450.99
25_E29_W1.7240.99
76_A80_A1.6520.99
122_D137_E1.6250.98
22_R25_E1.6120.98
29_W33_W1.5990.98
131_A136_G1.5840.98
65_A155_A1.5780.98
36_R40_D1.5670.98
20_A24_V1.5450.98
130_R141_A1.5190.98
129_G133_E1.4950.97
63_D67_K1.4850.97
31_A34_A1.4720.97
58_R61_A1.4310.97
144_R148_A1.4270.97
70_Q73_I1.4170.96
146_R150_L1.3820.96
131_A138_L1.3390.95
155_A158_A1.3310.95
57_R61_A1.2950.94
149_G153_E1.2860.94
33_W36_R1.2700.93
73_I77_R1.2380.93
77_R84_R1.2300.92
62_Q155_A1.2280.92
45_A48_E1.2260.92
22_R26_D1.2180.92
33_W37_D1.2100.92
70_Q74_A1.1930.91
48_E52_R1.1930.91
29_W32_K1.1780.91
37_D40_D1.1710.90
130_R135_A1.1700.90
59_Q62_Q1.1690.90
18_Y24_V1.1490.89
145_A149_G1.1480.89
44_A48_E1.1410.89
119_D122_D1.1360.89
25_E28_E1.1220.88
54_E61_A1.1100.88
150_L153_E1.1090.88
111_A155_A1.1080.88
93_Q96_A1.0890.87
130_R134_R1.0820.86
41_A45_A1.0770.86
48_E55_E1.0610.85
59_Q63_D1.0610.85
22_R30_Q1.0530.85
13_A16_W1.0520.85
45_A49_A1.0520.85
88_D91_R1.0450.84
14_L18_Y1.0260.83
69_A151_A1.0200.83
83_A87_A1.0040.82
138_L141_A0.9970.81
41_A47_A0.9860.81
40_D44_A0.9850.81
13_A17_G0.9850.81
46_A50_A0.9790.80
49_A53_A0.9730.80
123_V137_E0.9730.80
30_Q34_A0.9720.80
18_Y23_S0.9610.79
31_A35_E0.9600.79
69_A73_I0.9550.79
21_G29_W0.9550.79
25_E30_Q0.9480.78
135_A138_L0.9470.78
94_A98_A0.9460.78
38_A41_A0.9440.78
87_A132_D0.9410.77
68_D72_Q0.9390.77
67_K70_Q0.9320.77
42_E45_A0.9210.76
42_E46_A0.9180.76
93_Q97_L0.9090.75
24_V27_A0.9020.74
142_A145_A0.9010.74
68_D71_Q0.8980.74
154_R157_D0.8950.74
35_E39_A0.8930.74
16_W19_A0.8800.73
141_A144_R0.8790.73
8_L12_A0.8750.72
130_R133_E0.8710.72
88_D92_Q0.8690.72
75_A79_D0.8670.71
7_V10_L0.8630.71
26_D30_Q0.8610.71
6_I11_A0.8480.70
61_A64_E0.8480.70
150_L154_R0.8370.69
52_R56_Q0.8290.68
48_E51_A0.8230.67
29_W36_R0.8210.67
24_V28_E0.8110.66
6_I9_L0.8090.66
83_A86_A0.7920.64
90_L94_A0.7860.64
37_D45_A0.7850.64
117_A145_A0.7810.63
34_A38_A0.7740.63
56_Q59_Q0.7610.61
102_S105_A0.7570.61
117_A144_R0.7570.61
122_D134_R0.7570.61
71_Q74_A0.7510.60
85_A89_R0.7490.60
18_Y21_G0.7430.60
42_E47_A0.7240.58
29_W34_A0.7240.58
22_R27_A0.7220.57
128_L131_A0.7150.57
28_E32_K0.7120.56
26_D29_W0.7010.55
87_A91_R0.6990.55
25_E34_A0.6950.54
29_W37_D0.6930.54
141_A148_A0.6930.54
120_A137_E0.6880.54
20_A28_E0.6880.54
36_R43_A0.6870.54
55_E59_Q0.6780.53
19_A23_S0.6770.53
99_A110_A0.6720.52
122_D138_L0.6670.51
28_E38_A0.6630.51
102_S110_A0.6600.51
97_L124_L0.6550.50
9_L16_W0.6450.49
49_A56_Q0.6440.49
124_L137_E0.6430.49
124_L132_D0.6430.49
27_A34_A0.6410.49
133_E140_A0.6400.49
35_E38_A0.6390.48
12_A19_A0.6350.48
58_R76_A0.6350.48
67_K71_Q0.6340.48
53_A56_Q0.6330.48
93_Q127_L0.6320.48
60_A64_E0.6310.48
26_D33_W0.6310.48
81_A84_R0.6280.47
73_I76_A0.6260.47
16_W27_A0.6230.47
76_A144_R0.6210.47
46_A49_A0.6190.46
76_A79_D0.6190.46
21_G37_D0.6180.46
11_A15_L0.6090.45
63_D66_R0.6060.45
92_Q106_S0.6030.45
84_R140_A0.6020.44
19_A24_V0.6010.44
123_V141_A0.5990.44
25_E33_W0.5990.44
56_Q160_R0.5970.44
113_R127_L0.5950.44
7_V23_S0.5940.44
15_L18_Y0.5930.44
19_A25_E0.5900.43
19_A30_Q0.5870.43
65_A82_A0.5850.43
120_A123_V0.5810.42
86_A136_G0.5810.42
18_Y25_E0.5810.42
12_A22_R0.5770.42
70_Q77_R0.5760.42
72_Q75_A0.5750.42
134_R137_E0.5730.41
30_Q35_E0.5720.41
25_E36_R0.5700.41
65_A68_D0.5680.41
157_D160_R0.5660.41
11_A99_A0.5610.40
36_R45_A0.5590.40
77_R144_R0.5590.40
57_R62_Q0.5540.39
62_Q125_A0.5540.39
7_V11_A0.5530.39
99_A103_A0.5520.39
87_A90_L0.5480.39
89_R110_A0.5480.39
16_W22_R0.5470.39
19_A26_D0.5470.39
24_V34_A0.5450.39
84_R88_D0.5440.38
116_A149_G0.5430.38
51_A54_E0.5420.38
111_A117_A0.5390.38
23_S32_K0.5390.38
10_L13_A0.5380.38
20_A27_A0.5360.38
130_R142_A0.5340.37
115_Q118_A0.5320.37
6_I10_L0.5270.37
48_E71_Q0.5270.37
136_G140_A0.5260.37
24_V30_Q0.5250.37
36_R39_A0.5180.36
15_L22_R0.5160.36
60_A96_A0.5150.36
20_A23_S0.5130.35
72_Q89_R0.5120.35
16_W20_A0.5120.35
102_S108_P0.5120.35
83_A136_G0.5120.35
94_A128_L0.5020.34
33_W40_D0.5010.34
62_Q65_A0.5000.34
147_I151_A0.5000.34
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4q4gX 1 0 11.1 0.922 Contact Map
3rvyA 3 0.1677 4.8 0.934 Contact Map
4pj0R 1 0.1988 3.4 0.939 Contact Map
3rkoA 1 0.1863 3 0.94 Contact Map
2j69A 1 0.8137 2.8 0.941 Contact Map
1m56D 1 0.1118 2.7 0.941 Contact Map
2f93B 2 0.1056 2.7 0.941 Contact Map
5a2fA 1 0 2.6 0.942 Contact Map
4cgkA 3 0.7329 2.5 0.943 Contact Map
4u0qB 1 0 2.5 0.943 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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