GREMLIN Database
DUF2499 - Protein of unknown function (DUF2499)
PFAM: PF10693 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 89 (88)
Sequences: 583 (306)
Seq/√Len: 32.6
META: 0.726

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
48_V75_L3.5751.00
50_A56_W3.0821.00
53_A69_V2.2820.99
42_A82_T2.2120.99
75_L78_L2.0190.98
49_S73_A2.0160.98
47_L50_A2.0110.98
47_L56_W1.9890.98
34_P37_R1.9490.97
25_W29_E1.7640.95
56_W60_D1.6060.92
24_I39_L1.5900.92
13_S16_E1.5800.92
23_L27_Y1.5630.91
81_C85_L1.5070.89
23_L26_R1.5060.89
8_I12_S1.4390.87
72_Q76_T1.4360.87
38_G41_L1.4170.86
9_I52_C1.4060.86
17_W24_I1.3930.85
3_S7_W1.3910.85
3_S6_T1.3460.83
70_T74_A1.3330.83
61_N80_N1.3280.82
44_L48_V1.3220.82
9_I84_A1.3110.82
21_I60_D1.2930.81
2_L7_W1.2810.80
28_G37_R1.2640.79
4_L8_I1.2460.78
49_S75_L1.2240.77
63_P66_E1.2220.77
25_W37_R1.2130.76
29_E41_L1.2060.76
47_L51_M1.2040.76
69_V72_Q1.1910.75
28_G34_P1.1750.74
7_W10_H1.1740.74
45_P79_G1.1440.72
67_W70_T1.1270.71
46_A54_C1.1270.71
38_G42_A1.1130.70
61_N72_Q1.0960.69
58_F84_A1.0630.66
31_T34_P1.0620.66
46_A56_W1.0540.65
32_G64_S1.0440.65
4_L88_W1.0370.64
52_C70_T1.0140.62
2_L6_T0.9810.60
64_S74_A0.9620.58
58_F67_W0.9580.58
14_V70_T0.9460.57
25_W58_F0.9420.57
81_C84_A0.9340.56
49_S53_A0.9290.56
28_G63_P0.9280.55
58_F66_E0.9200.55
19_A77_L0.8900.52
11_I88_W0.8840.52
51_M56_W0.8770.51
44_L83_L0.8690.51
28_G33_N0.8550.49
36_W54_C0.8530.49
50_A60_D0.8520.49
16_E20_A0.8400.48
66_E81_C0.8330.48
17_W83_L0.8230.47
6_T50_A0.8080.46
49_S54_C0.8080.46
34_P64_S0.8070.46
14_V48_V0.8050.45
19_A25_W0.8010.45
53_A72_Q0.7980.45
26_R44_L0.7970.45
28_G31_T0.7970.45
30_V33_N0.7960.45
23_L43_M0.7950.45
25_W30_V0.7900.44
66_E78_L0.7790.43
18_I51_M0.7560.41
74_A77_L0.7510.41
61_N73_A0.7440.40
26_R85_L0.7400.40
84_A88_W0.7370.40
22_W26_R0.7340.40
54_C58_F0.7250.39
26_R30_V0.7240.39
5_P27_Y0.7230.39
19_A23_L0.7060.37
5_P52_C0.6910.36
8_I58_F0.6900.36
26_R32_G0.6800.35
53_A76_T0.6720.35
15_I19_A0.6500.33
5_P63_P0.6500.33
25_W34_P0.6490.33
44_L88_W0.6490.33
61_N69_V0.6450.33
14_V68_L0.6420.33
46_A83_L0.6400.32
43_M83_L0.6340.32
12_S71_L0.6290.32
26_R64_S0.6250.31
72_Q80_N0.6240.31
38_G60_D0.6150.31
76_T80_N0.6120.30
19_A84_A0.6010.30
58_F82_T0.5970.29
67_W83_L0.5960.29
40_A47_L0.5910.29
11_I17_W0.5750.28
26_R62_A0.5730.28
31_T37_R0.5730.28
11_I74_A0.5660.27
63_P88_W0.5620.27
51_M60_D0.5570.27
46_A67_W0.5550.27
3_S10_H0.5530.26
46_A58_F0.5420.26
48_V52_C0.5400.26
46_A60_D0.5170.24
63_P78_L0.5120.24
55_T59_F0.5100.24
24_I36_W0.5100.24
18_I35_A0.5070.24
6_T10_H0.5070.24
27_Y31_T0.5060.24
32_G37_R0.5040.23
11_I62_A0.5010.23
2_L10_H0.5000.23
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3rzeA 1 0.3034 3.4 0.944 Contact Map
4n4rB 1 0.3708 2.9 0.947 Contact Map
3vw7A 1 0.4382 2.9 0.947 Contact Map
2rh1A 1 0.3146 2.7 0.948 Contact Map
2hi7B 1 0.236 2.7 0.948 Contact Map
4ogqG 1 0.4157 2 0.951 Contact Map
4mbsA 1 0.4157 1.9 0.951 Contact Map
1q90G 1 0.3371 1.9 0.952 Contact Map
2qjvA 4 0.4719 1.6 0.954 Contact Map
4mxeA 4 0.3371 1.6 0.954 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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