GREMLIN Database
DUF2478 - Protein of unknown function (DUF2478)
PFAM: PF10649 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 159 (158)
Sequences: 2862 (2188)
Seq/√Len: 174.1
META: 0.843

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
49_D58_R3.4671.00
20_A98_I3.3061.00
19_F156_L2.8871.00
29_R95_D2.7591.00
139_R148_E2.7581.00
28_L95_D2.7271.00
36_R51_R2.7211.00
135_L139_R2.5771.00
77_A81_E2.3211.00
152_D155_A2.2871.00
30_V96_L2.2871.00
52_D55_T2.2081.00
51_R58_R2.1531.00
13_D101_K2.0301.00
118_E122_A2.0281.00
22_R157_L2.0141.00
153_R157_L1.9721.00
87_E118_E1.9681.00
65_L69_A1.9281.00
31_G94_A1.9051.00
22_R153_R1.8781.00
86_V90_L1.8731.00
13_D37_N1.8331.00
96_L127_L1.8161.00
33_V50_L1.8121.00
104_K134_N1.7521.00
86_V89_A1.7481.00
49_D60_R1.6891.00
118_E121_A1.6531.00
59_I78_A1.6021.00
154_D157_L1.5961.00
136_D140_A1.5881.00
7_A132_E1.5751.00
22_R26_R1.5691.00
52_D57_E1.5601.00
34_V53_L1.4991.00
116_I128_T1.4971.00
63_Q66_G1.4851.00
87_E115_A1.4781.00
104_K108_E1.4631.00
82_A112_F1.4381.00
23_L28_L1.4251.00
48_M62_S1.4201.00
14_T18_R1.3841.00
20_A24_R1.3821.00
154_D158_A1.3681.00
75_D78_A1.3211.00
66_G69_A1.2881.00
17_A36_R1.2871.00
37_N48_M1.2801.00
107_A137_A1.2611.00
66_G72_C1.2461.00
97_V119_A1.2451.00
150_P155_A1.2441.00
17_A21_E1.2371.00
36_R58_R1.2371.00
2_A149_L1.2331.00
23_L96_L1.2291.00
74_L105_L1.2261.00
15_L19_F1.2211.00
16_L98_I1.2021.00
13_D100_N1.1881.00
7_A10_G1.1851.00
81_E85_A1.1831.00
101_K129_A1.1761.00
97_V124_I1.1571.00
76_P80_A1.1551.00
19_F157_L1.1551.00
21_E24_R1.1441.00
142_A146_A1.1431.00
80_A111_G1.1391.00
140_A143_G1.0991.00
6_Y12_K1.0741.00
20_A34_V1.0701.00
57_E81_E1.0641.00
38_V47_G1.0611.00
18_R153_R1.0581.00
63_Q69_A1.0350.99
155_A158_A1.0300.99
6_Y101_K1.0140.99
136_D139_R1.0110.99
17_A34_V0.9970.99
3_A6_Y0.9940.99
142_A145_L0.9930.99
135_L148_E0.9930.99
11_G14_T0.9910.99
33_V86_V0.9890.99
79_L105_L0.9750.99
78_A81_E0.9640.99
7_A11_G0.9530.99
91_A118_E0.9370.99
102_F128_T0.9330.99
69_A72_C0.9150.99
147_V157_L0.9040.99
8_P11_G0.8990.98
5_V148_E0.8890.98
85_A89_A0.8880.98
19_F127_L0.8870.98
45_R48_M0.8870.98
55_T58_R0.8710.98
145_L148_E0.8660.98
99_V116_I0.8640.98
113_R117_A0.8580.98
40_R43_G0.8520.98
96_L124_I0.8440.98
98_I127_L0.8310.97
80_A83_A0.8290.97
52_D89_A0.8290.97
119_A126_V0.8170.97
24_R51_R0.7990.97
33_V115_A0.7940.97
74_L79_L0.7930.97
131_P134_N0.7890.97
85_A88_R0.7890.97
117_A121_A0.7870.96
65_L71_G0.7810.96
84_A88_R0.7770.96
90_L97_V0.7730.96
21_E25_A0.7710.96
22_R25_A0.7660.96
35_Q104_K0.7640.96
24_R30_V0.7580.96
4_V15_L0.7570.96
12_K100_N0.7550.96
114_D118_E0.7550.96
13_D16_L0.7520.95
19_F98_I0.7510.95
12_K101_K0.7500.95
15_L153_R0.7490.95
117_A120_L0.7450.95
76_P105_L0.7430.95
150_P158_A0.7410.95
105_L110_R0.7370.95
35_Q119_A0.7370.95
148_E153_R0.7350.95
61_I78_A0.7280.95
86_V112_F0.7280.95
4_V12_K0.7260.94
101_K138_W0.7190.94
59_I85_A0.7140.94
150_P153_R0.7110.94
138_W142_A0.7080.94
103_G106_E0.7030.94
63_Q73_R0.6940.93
147_V150_P0.6930.93
35_Q112_F0.6920.93
131_P142_A0.6900.93
105_L108_E0.6890.93
31_G54_A0.6850.93
145_L149_L0.6810.92
68_G72_C0.6760.92
101_K117_A0.6760.92
13_D35_Q0.6720.92
60_R63_Q0.6680.92
16_L23_L0.6670.92
90_L122_A0.6640.91
50_L54_A0.6610.91
43_G157_L0.6610.91
62_S65_L0.6600.91
145_L158_A0.6560.91
83_A112_F0.6490.90
65_L73_R0.6450.90
73_R100_N0.6400.90
35_Q97_V0.6400.90
87_E91_A0.6360.90
33_V85_A0.6310.89
90_L124_I0.6300.89
30_V97_V0.6290.89
25_A56_G0.6270.89
153_R158_A0.6270.89
49_D63_Q0.6240.89
65_L68_G0.6230.89
69_A74_L0.6220.89
100_N107_A0.6200.88
111_G114_D0.6180.88
6_Y11_G0.6180.88
12_K15_L0.6170.88
97_V122_A0.6150.88
106_E111_G0.6140.88
26_R157_L0.6130.88
39_R47_G0.6120.88
5_V12_K0.6090.88
130_V138_W0.6020.87
51_R56_G0.5980.87
12_K131_P0.5980.87
66_G73_R0.5980.87
40_R47_G0.5950.86
99_V115_A0.5880.86
9_G12_K0.5860.85
16_L129_A0.5800.85
147_V151_P0.5750.84
33_V52_D0.5730.84
15_L151_P0.5720.84
138_W146_A0.5710.84
69_A73_R0.5700.84
105_L131_P0.5700.84
80_A84_A0.5690.84
35_Q109_G0.5690.84
48_M59_I0.5680.84
57_E85_A0.5680.84
86_V115_A0.5670.84
6_Y131_P0.5640.83
23_L26_R0.5640.83
150_P156_L0.5620.83
139_R142_A0.5610.83
130_V135_L0.5590.83
147_V154_D0.5570.82
114_D123_G0.5570.82
63_Q108_E0.5550.82
31_G115_A0.5530.82
80_A110_R0.5500.82
93_G122_A0.5450.81
84_A87_E0.5450.81
83_A87_E0.5420.81
151_P156_L0.5410.81
128_T131_P0.5410.81
78_A82_A0.5400.81
3_A9_G0.5370.80
49_D110_R0.5350.80
148_E154_D0.5330.80
35_Q101_K0.5280.79
150_P157_L0.5230.78
72_C119_A0.5200.78
99_V142_A0.5180.78
93_G124_I0.5170.78
39_R45_R0.5130.77
18_R21_E0.5100.77
21_E51_R0.5100.77
107_A134_N0.5080.76
15_L156_L0.5080.76
143_G146_A0.5070.76
50_L79_L0.5060.76
148_E156_L0.5050.76
156_L159_W0.5030.76
5_V9_G0.5020.76
83_A115_A0.5020.76
33_V101_K0.5000.75
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1xx6A 4 0.805 98.6 0.761 Contact Map
3e2iA 3 0.7673 98.6 0.762 Contact Map
2j9rA 3 0.7421 98.6 0.762 Contact Map
2b8tA 3 0.8679 98.5 0.767 Contact Map
2r8rA 1 0.8931 98.2 0.786 Contact Map
2vhjA 3 0.7484 98.2 0.786 Contact Map
3jvvA 3 0.805 98.1 0.791 Contact Map
2ewvA 3 0.8239 98.1 0.792 Contact Map
3bosA 2 0.7358 98.1 0.793 Contact Map
1ye8A 1 0.9371 98 0.796 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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