GREMLIN Database
CsgF - Type VIII secretion system (T8SS), CsgF protein
PFAM: PF10614 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 131 (128)
Sequences: 3223 (2086)
Seq/√Len: 184.4
META: 0.889

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
46_N50_D3.2981.00
61_T64_A3.1641.00
105_E118_T2.3591.00
47_R51_P2.1491.00
40_I44_A2.1101.00
40_I43_Q2.1051.00
22_K31_N2.0061.00
25_N29_G1.9421.00
44_A47_R1.8891.00
45_Q49_K1.8601.00
39_L43_Q1.8411.00
44_A48_K1.7851.00
42_A46_N1.7811.00
42_A45_Q1.7021.00
80_K84_D1.6861.00
37_Y41_E1.6681.00
27_S37_Y1.6571.00
41_E45_Q1.6531.00
49_K53_A1.6191.00
70_L74_I1.6181.00
49_K52_K1.6161.00
35_S39_L1.6151.00
50_D53_A1.5831.00
43_Q47_R1.5811.00
45_Q48_K1.5531.00
48_K51_P1.5471.00
114_T129_T1.5391.00
79_S83_V1.5071.00
112_G129_T1.4861.00
33_Y37_Y1.4701.00
119_D122_T1.4401.00
43_Q46_N1.4381.00
58_A61_T1.4261.00
119_D124_T1.4231.00
96_T105_E1.3991.00
12_S15_S1.3781.00
74_I86_M1.3721.00
39_L42_A1.3441.00
46_N49_K1.3431.00
37_Y40_I1.3201.00
60_N68_R1.2821.00
35_S38_L1.2721.00
118_T125_T1.2691.00
81_Q87_F1.2511.00
9_L13_S1.2481.00
58_A64_A1.2471.00
55_E59_E1.2431.00
98_T103_T1.2341.00
98_T101_G1.2301.00
47_R52_K1.2241.00
65_K72_S1.2241.00
46_N51_P1.2201.00
25_N38_L1.2091.00
60_N64_A1.1751.00
11_A14_A1.1721.00
96_T103_T1.1671.00
109_T112_G1.1661.00
7_L11_A1.1561.00
116_T127_T1.1431.00
34_N38_L1.1421.00
99_L102_N1.1201.00
54_A58_A1.0981.00
66_F75_Y1.0941.00
109_T114_T1.0911.00
107_T116_T1.0881.00
36_H40_I1.0751.00
10_L14_A1.0721.00
88_G91_G1.0661.00
59_E68_R1.0441.00
82_L97_F1.0361.00
70_L82_L1.0341.00
6_L9_L1.0161.00
10_L13_S1.0140.99
5_L9_L0.9890.99
29_G32_G0.9800.99
55_E58_A0.9790.99
42_A48_K0.9750.99
46_N52_K0.9720.99
105_E116_T0.9710.99
68_R72_S0.9580.99
62_T71_E0.9570.99
29_G33_Y0.9530.99
27_S35_S0.9480.99
62_T68_R0.9470.99
43_Q48_K0.9400.99
48_K52_K0.9340.99
62_T65_K0.9260.99
8_L12_S0.9260.99
32_G36_H0.9250.99
36_H39_L0.9230.99
39_L45_Q0.9170.99
29_G34_N0.9140.99
42_A50_D0.9110.99
126_T131_P0.8920.99
108_K111_D0.8920.99
40_I45_Q0.8860.99
34_N37_Y0.8770.99
33_Y36_H0.8760.99
97_F102_N0.8760.99
10_L16_A0.8750.98
39_L44_A0.8670.98
18_E21_Y0.8650.98
4_L9_L0.8640.98
69_N73_R0.8610.98
116_T125_T0.8440.98
86_M100_G0.8380.98
9_L12_S0.8210.98
73_R76_A0.8160.98
65_K71_E0.8090.98
97_F101_G0.7920.97
94_S108_K0.7730.97
42_A47_R0.7690.97
101_G121_D0.7630.96
103_T126_T0.7600.96
10_L15_S0.7590.96
41_E44_A0.7590.96
7_L10_L0.7530.96
44_A51_P0.7410.96
35_S41_E0.7410.96
8_L11_A0.7370.96
115_L130_V0.7330.96
92_L95_G0.7320.96
78_L81_Q0.7200.95
98_T120_T0.7030.94
128_I131_P0.6940.94
107_T114_T0.6940.94
32_G35_S0.6920.94
97_F104_I0.6890.94
7_L12_S0.6890.94
76_A80_K0.6750.93
100_G104_I0.6700.93
7_L15_S0.6660.93
57_E60_N0.6660.93
4_L8_L0.6610.92
5_L8_L0.6600.92
98_T104_I0.6560.92
45_Q51_P0.6540.92
6_L11_A0.6500.92
46_N53_A0.6480.92
24_K29_G0.6480.92
56_R59_E0.6470.92
54_A57_E0.6470.92
69_N74_I0.6460.92
41_E48_K0.6360.91
38_L42_A0.6330.91
27_S41_E0.6330.91
106_Y110_G0.6290.90
30_G33_Y0.6270.90
117_I120_T0.6270.90
40_I51_P0.6210.90
82_L86_M0.6210.90
44_A49_K0.6180.90
13_S16_A0.6180.90
97_F106_Y0.6160.90
73_R77_Q0.6150.89
91_G94_S0.6060.89
6_L15_S0.6040.89
60_N69_N0.6040.89
71_E83_V0.6020.88
6_L12_S0.5990.88
83_V86_M0.5970.88
106_Y109_T0.5900.87
6_L10_L0.5880.87
99_L103_T0.5880.87
100_G105_E0.5850.87
41_E47_R0.5810.87
25_N37_Y0.5800.87
40_I46_N0.5780.86
92_L97_F0.5670.85
117_I128_I0.5650.85
83_V104_I0.5630.85
8_L13_S0.5600.85
21_Y29_G0.5580.84
35_S40_I0.5580.84
61_T65_K0.5540.84
56_R65_K0.5530.84
70_L119_D0.5410.83
8_L15_S0.5370.82
77_Q80_K0.5340.82
107_T115_L0.5340.82
56_R80_K0.5310.81
77_Q81_Q0.5290.81
114_T127_T0.5280.81
103_T120_T0.5230.80
72_S78_L0.5220.80
4_L15_S0.5180.80
17_Q21_Y0.5160.80
11_A15_S0.5160.80
5_L14_A0.5070.78
35_S43_Q0.5060.78
97_F105_E0.5010.78
99_L120_T0.5000.77
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3rfzB 2 0.8244 17.3 0.925 Contact Map
3fcgA 2 0.5191 11.7 0.93 Contact Map
3fipA 1 0.7786 11.3 0.931 Contact Map
3fsoA 1 0.2901 10.3 0.932 Contact Map
2kncB 1 0.2366 5 0.941 Contact Map
3e9tA 1 0.2901 4.7 0.942 Contact Map
3iedA 1 0.6489 3.6 0.945 Contact Map
3rb5A 2 0.2748 3.3 0.946 Contact Map
2kywA 1 0.2595 3.1 0.947 Contact Map
1uxxX 1 0.5038 2.9 0.948 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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