GREMLIN Database
DUF2332 - Uncharacterized protein conserved in bacteria (DUF2332)
PFAM: PF10094 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 335 (330)
Sequences: 4275 (3398)
Seq/√Len: 187.1
META: 0.796

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
207_R222_R5.4891.00
195_L202_D4.4581.00
199_D202_D3.6311.00
119_G292_L3.2831.00
239_D272_E3.2651.00
197_V225_I3.1651.00
203_R225_I3.1601.00
147_D187_R2.8421.00
53_V85_F2.8391.00
120_L146_W2.7021.00
52_A96_L2.6881.00
150_R166_R2.6771.00
248_L283_A2.6411.00
136_V276_F2.5641.00
12_A24_R2.5181.00
245_P275_R2.4031.00
23_A50_L2.4031.00
248_L257_T2.3451.00
327_D331_R2.2811.00
87_A91_A2.1961.00
58_L204_L2.1831.00
5_R28_D2.1191.00
269_P272_E2.0461.00
190_C243_W2.0191.00
203_R229_R2.0181.00
185_V255_A1.9901.00
259_V280_L1.9871.00
241_A272_E1.9591.00
152_D168_A1.9391.00
148_R231_D1.9211.00
294_L326_A1.9171.00
190_C238_G1.9031.00
188_A236_V1.9031.00
152_D166_R1.8981.00
225_I229_R1.8971.00
134_L247_L1.8821.00
172_R334_E1.8581.00
277_A281_A1.8551.00
8_A24_R1.8421.00
93_R97_R1.8321.00
86_R90_L1.8321.00
163_S227_L1.8191.00
145_L233_P1.7981.00
132_A255_A1.7821.00
82_W86_R1.7691.00
244_L259_V1.7631.00
113_S149_Y1.7631.00
145_L231_D1.7521.00
92_H95_E1.7261.00
29_D86_R1.7041.00
132_A186_S1.6871.00
99_L208_A1.6701.00
210_V221_L1.6651.00
264_V268_L1.6591.00
5_R24_R1.6571.00
152_D157_R1.6501.00
193_N237_R1.6481.00
241_A279_A1.6421.00
134_L257_T1.6291.00
113_S143_N1.6021.00
172_R332_W1.5981.00
274_A278_A1.5911.00
118_P153_Y1.5781.00
19_Y50_L1.5581.00
20_A24_R1.5571.00
111_G220_R1.5401.00
52_A99_L1.5391.00
126_R319_R1.5331.00
325_R334_E1.5311.00
270_A274_A1.5151.00
59_A204_L1.5081.00
123_L256_T1.4991.00
25_A82_W1.4971.00
164_P227_L1.4901.00
242_D272_E1.4561.00
236_V243_W1.4491.00
120_L260_F1.4361.00
242_D275_R1.4131.00
277_A291_W1.4131.00
12_A20_A1.4051.00
113_S151_Y1.4011.00
188_A247_L1.3991.00
136_V240_A1.3731.00
207_R218_L1.3571.00
165_V223_A1.3551.00
218_L222_R1.3441.00
207_R221_L1.3421.00
13_R20_A1.3421.00
5_R27_A1.3401.00
221_L225_I1.3391.00
138_A235_V1.3261.00
152_D155_G1.3241.00
21_R25_A1.3161.00
219_A222_R1.3141.00
311_T318_R1.3121.00
119_G323_L1.3061.00
238_G243_W1.3031.00
55_A204_L1.2981.00
109_E217_R1.2841.00
265_L276_F1.2791.00
122_A126_R1.2771.00
144_L189_G1.2761.00
245_P279_A1.2651.00
190_C247_L1.2611.00
14_G20_A1.2561.00
136_V264_V1.2521.00
54_H58_L1.2511.00
116_L262_S1.2411.00
150_R157_R1.2411.00
284_G313_W1.2401.00
292_L310_L1.2391.00
245_P282_A1.2371.00
263_I306_A1.2361.00
308_L324_A1.2271.00
226_A230_A1.2231.00
134_L188_A1.2211.00
9_E24_R1.2171.00
57_V72_A1.2171.00
240_A264_V1.2011.00
188_A234_R1.1971.00
241_A276_F1.1941.00
243_W246_A1.1931.00
88_A92_H1.1931.00
122_A125_R1.1871.00
219_A223_A1.1861.00
115_A294_L1.1811.00
244_L279_A1.1811.00
55_A99_L1.1801.00
133_L146_W1.1781.00
71_S74_G1.1771.00
89_L96_L1.1741.00
256_T290_A1.1711.00
272_E275_R1.1661.00
129_L256_T1.1661.00
226_A229_R1.1651.00
197_V229_R1.1621.00
265_L273_R1.1621.00
284_G289_L1.1521.00
132_A185_V1.1411.00
7_Q11_C1.1401.00
197_V233_P1.1231.00
35_L93_R1.1121.00
324_A333_V1.1001.00
247_L250_E1.0971.00
290_A310_L1.0891.00
107_T139_S1.0811.00
49_L100_L1.0791.00
120_L258_V1.0771.00
34_G37_A1.0741.00
132_A257_T1.0691.00
107_T217_R1.0601.00
236_V247_L1.0581.00
138_A260_F1.0571.00
51_G209_Y1.0571.00
197_V202_D1.0571.00
123_L127_T1.0561.00
123_L258_V1.0481.00
11_C15_G1.0411.00
203_R226_A1.0411.00
6_R10_A1.0291.00
276_F280_L1.0281.00
261_H291_W1.0251.00
180_A183_E1.0231.00
16_S213_D1.0191.00
131_L256_T1.0171.00
47_L105_T1.0141.00
240_A268_L1.0121.00
251_A257_T1.0101.00
21_R81_A1.0040.99
63_P87_A1.0030.99
290_A312_L0.9990.99
117_L158_W0.9950.99
261_H295_E0.9940.99
55_A208_A0.9910.99
263_I266_Q0.9900.99
54_H218_L0.9890.99
26_V36_L0.9880.99
238_G242_D0.9870.99
135_E260_F0.9770.99
213_D218_L0.9740.99
146_W260_F0.9700.99
265_L268_L0.9600.99
22_L50_L0.9520.99
278_A282_A0.9510.99
98_A101_A0.9400.99
186_S251_A0.9370.99
65_L69_Y0.9350.99
47_L209_Y0.9350.99
15_G214_Q0.9310.99
279_A282_A0.9270.99
77_D80_A0.9230.99
143_N262_S0.9200.99
134_L248_L0.9180.99
216_E220_R0.9180.99
98_A102_T0.9140.99
133_L260_F0.9130.99
288_P314_P0.9110.99
29_D34_G0.9100.99
97_R101_A0.9090.99
38_R97_R0.9070.99
154_G157_R0.9050.99
24_R28_D0.8990.99
39_A43_D0.8980.99
107_T329_H0.8940.99
305_G325_R0.8910.99
306_A328_P0.8890.99
238_G246_A0.8820.99
61_R95_E0.8800.99
95_E98_A0.8790.99
211_W329_H0.8770.99
197_V228_A0.8760.99
35_L38_R0.8760.99
107_T211_W0.8750.99
262_S294_L0.8710.99
327_D332_W0.8690.99
239_D242_D0.8680.98
62_A88_A0.8660.98
5_R9_E0.8650.98
102_T205_W0.8640.98
117_L151_Y0.8620.98
120_L131_L0.8620.98
127_T256_T0.8610.98
309_R318_R0.8610.98
73_G76_P0.8600.98
244_L276_F0.8590.98
4_F30_L0.8590.98
281_A311_T0.8580.98
29_D82_W0.8570.98
124_A131_L0.8530.98
132_A252_P0.8530.98
264_V276_F0.8510.98
217_R329_H0.8490.98
2_E6_R0.8470.98
25_A29_D0.8460.98
9_E13_R0.8460.98
111_G114_A0.8460.98
146_W182_P0.8430.98
53_V57_V0.8420.98
200_P229_R0.8410.98
211_W217_R0.8400.98
204_L208_A0.8340.98
36_L49_L0.8330.98
26_V50_L0.8320.98
261_H293_S0.8300.98
171_W333_V0.8300.98
57_V69_Y0.8260.98
176_P179_L0.8240.98
104_P209_Y0.8240.98
294_L306_A0.8240.98
216_E219_A0.8230.98
148_R230_A0.8150.98
204_L207_R0.8150.98
122_A310_L0.8120.98
309_R320_V0.8080.98
7_Q47_L0.8050.98
82_W85_F0.7990.97
116_L120_L0.7980.97
73_G77_D0.7980.97
32_L82_W0.7950.97
132_A251_A0.7940.97
114_A169_C0.7920.97
243_W247_L0.7890.97
203_R207_R0.7880.97
3_A44_A0.7860.97
136_V259_V0.7840.97
311_T316_G0.7800.97
33_L37_A0.7780.97
54_H71_S0.7780.97
263_I267_Y0.7740.97
261_H276_F0.7740.97
175_V178_P0.7690.97
168_A216_E0.7660.97
10_A14_G0.7610.97
160_D163_S0.7600.97
148_R160_D0.7600.97
130_P185_V0.7590.96
268_L276_F0.7580.96
308_L323_L0.7580.96
142_L224_A0.7580.96
153_Y156_G0.7530.96
297_P307_E0.7510.96
8_A12_A0.7510.96
68_Y71_S0.7480.96
275_R278_A0.7440.96
56_L99_L0.7430.96
310_L313_W0.7430.96
150_R160_D0.7420.96
118_P335_W0.7400.96
68_Y84_A0.7390.96
165_V227_L0.7380.96
120_L133_L0.7350.96
131_L260_F0.7350.96
128_G256_T0.7330.96
38_R93_R0.7300.96
126_R312_L0.7240.95
215_P218_L0.7220.95
108_N139_S0.7210.95
11_C14_G0.7210.95
114_A167_L0.7210.95
39_A45_L0.7190.95
253_A286_D0.7180.95
127_T312_L0.7160.95
109_E211_W0.7150.95
215_P219_A0.7130.95
168_A220_R0.7120.95
134_L186_S0.7100.95
326_A333_V0.7100.95
115_A326_A0.7090.95
55_A201_D0.7080.95
12_A15_G0.7070.95
106_Q267_Y0.7050.95
263_I294_L0.7030.95
64_P87_A0.7020.95
153_Y169_C0.7010.95
107_T267_Y0.7000.95
54_H213_D0.7000.95
131_L182_P0.6990.94
59_A95_E0.6990.94
222_R226_A0.6960.94
210_V218_L0.6950.94
268_L272_E0.6950.94
119_G262_S0.6940.94
188_A235_V0.6910.94
111_G327_D0.6910.94
254_G286_D0.6900.94
292_L323_L0.6870.94
276_F279_A0.6870.94
145_L227_L0.6860.94
53_V88_A0.6850.94
312_L319_R0.6850.94
111_G216_E0.6800.94
4_F26_V0.6760.93
297_P325_R0.6750.93
192_L264_V0.6730.93
186_S255_A0.6700.93
171_W175_V0.6690.93
56_L92_H0.6660.93
93_R96_L0.6640.93
84_A87_A0.6640.93
134_L243_W0.6630.93
68_Y72_A0.6610.93
310_L315_G0.6610.93
43_D48_R0.6580.93
146_W184_V0.6580.93
49_L53_V0.6540.92
125_R180_A0.6530.92
236_V246_A0.6520.92
275_R279_A0.6500.92
139_S217_R0.6480.92
123_L292_L0.6420.92
216_E331_R0.6400.91
251_A255_A0.6380.91
308_L313_W0.6370.91
123_L131_L0.6360.91
171_W177_P0.6350.91
7_Q12_A0.6350.91
15_G327_D0.6340.91
130_P183_E0.6330.91
110_V167_L0.6320.91
30_L34_G0.6320.91
293_S318_R0.6310.91
3_A46_P0.6300.91
23_A26_V0.6290.91
133_L187_R0.6290.91
236_V250_E0.6290.91
103_R209_Y0.6290.91
61_R92_H0.6280.91
265_L277_A0.6280.91
267_Y329_H0.6270.91
266_Q296_P0.6260.91
18_L22_L0.6260.91
272_E276_F0.6230.90
234_R250_E0.6220.90
26_V82_W0.6220.90
206_L221_L0.6210.90
30_L37_A0.6190.90
19_Y22_L0.6190.90
186_S252_P0.6180.90
269_P274_A0.6170.90
15_G20_A0.6170.90
314_P320_V0.6170.90
257_T289_L0.6130.90
150_R158_W0.6130.90
293_S309_R0.6070.89
44_A266_Q0.6060.89
198_T232_P0.6060.89
197_V207_R0.6050.89
105_T267_Y0.6040.89
220_R227_L0.6040.89
278_A281_A0.6000.89
85_F96_L0.6000.89
116_L260_F0.6000.89
124_A127_T0.5970.88
252_P287_R0.5960.88
150_R159_G0.5960.88
129_L254_G0.5950.88
103_R192_L0.5920.88
44_A267_Y0.5920.88
200_P203_R0.5920.88
53_V96_L0.5900.88
64_P91_A0.5890.88
121_L158_W0.5880.88
109_E220_R0.5860.87
259_V289_L0.5820.87
293_S296_P0.5810.87
30_L33_L0.5780.87
43_D104_P0.5780.87
289_L313_W0.5770.87
94_D97_R0.5760.87
135_E261_H0.5760.87
60_G92_H0.5740.86
6_R9_E0.5730.86
240_A243_W0.5720.86
39_A42_G0.5690.86
115_A118_P0.5680.86
16_S214_Q0.5680.86
138_A189_G0.5680.86
291_W296_P0.5670.86
36_L89_L0.5640.85
131_L146_W0.5640.85
149_Y167_L0.5610.85
293_S311_T0.5610.85
194_P237_R0.5600.85
25_A28_D0.5600.85
150_R163_S0.5590.85
294_L308_L0.5590.85
123_L290_A0.5580.85
241_A275_R0.5570.85
106_Q112_R0.5560.85
71_S218_L0.5560.85
279_A283_A0.5540.84
197_V203_R0.5530.84
69_Y85_F0.5530.84
55_A95_E0.5500.84
291_W295_E0.5490.84
136_V265_L0.5480.84
6_R11_C0.5440.83
200_P204_L0.5440.83
45_L48_R0.5420.83
19_Y69_Y0.5420.83
4_F23_A0.5410.83
322_L325_R0.5380.83
186_S234_R0.5380.83
108_N211_W0.5370.83
308_L326_A0.5360.82
294_L323_L0.5350.82
198_T229_R0.5340.82
186_S247_L0.5340.82
280_L284_G0.5330.82
68_Y75_D0.5330.82
121_L182_P0.5310.82
136_V190_C0.5310.82
186_S257_T0.5310.82
7_Q14_G0.5290.82
192_L239_D0.5280.81
184_V187_R0.5260.81
163_S166_R0.5250.81
55_A59_A0.5250.81
9_E12_A0.5240.81
40_P43_D0.5240.81
44_A47_L0.5240.81
30_L36_L0.5240.81
284_G290_A0.5230.81
85_F89_L0.5230.81
188_A251_A0.5220.81
110_V224_A0.5210.81
32_L89_L0.5190.80
70_P74_G0.5170.80
258_V292_L0.5160.80
64_P84_A0.5160.80
217_R267_Y0.5150.80
277_A280_L0.5140.80
214_Q218_L0.5140.80
139_S211_W0.5140.80
25_A81_A0.5140.80
310_L316_G0.5120.79
11_C51_G0.5110.79
145_L148_R0.5100.79
99_L205_W0.5090.79
54_H212_P0.5090.79
318_R322_L0.5080.79
135_E143_N0.5070.79
108_N217_R0.5070.79
31_E34_G0.5070.79
241_A245_P0.5060.79
7_Q23_A0.5050.79
280_L291_W0.5040.78
68_Y73_G0.5040.78
18_L213_D0.5030.78
119_G122_A0.5030.78
121_L151_Y0.5030.78
323_L335_W0.5010.78
243_W268_L0.5000.78
57_V66_A0.5000.78
159_G163_S0.5000.78
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2p8jA 2 0.4955 82.6 0.955 Contact Map
3jwgA 1 0.4955 74.6 0.958 Contact Map
4xcxA 1 0.4955 70.2 0.96 Contact Map
1af7A 1 0.7582 64.3 0.961 Contact Map
3ihtA 2 0.4478 63.8 0.961 Contact Map
3dr5A 2 0.6149 52.7 0.964 Contact Map
2h00A 1 0.5881 48.8 0.965 Contact Map
2hnkA 2 0.5642 48.2 0.965 Contact Map
4azsA 6 0.5552 45.9 0.965 Contact Map
3h2bA 2 0.5433 44.7 0.965 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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