GREMLIN Database
DUF2322 - Uncharacterized protein conserved in bacteria (DUF2322)
PFAM: PF10084 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 98 (98)
Sequences: 984 (583)
Seq/√Len: 58.9
META: 0.792

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
75_N78_K2.9871.00
21_D25_A2.9641.00
46_L61_G2.9541.00
36_K65_F2.8881.00
62_L69_T2.8701.00
71_D78_K2.6941.00
49_K60_E2.6881.00
8_L42_V2.5961.00
53_I97_V2.5831.00
32_N37_Q2.3261.00
4_N7_Q2.3211.00
20_L26_V2.2181.00
60_E64_L2.1651.00
70_A74_A2.1451.00
87_A91_G1.9201.00
44_N48_Q1.8291.00
18_E29_T1.7960.99
68_H71_D1.6400.99
42_V64_L1.6350.99
16_A29_T1.5830.99
14_L40_L1.5150.98
4_N64_L1.4490.97
18_E26_V1.4430.97
18_E98_K1.4330.97
2_A6_K1.3990.97
10_S41_A1.3510.96
91_G94_T1.2980.95
26_V29_T1.2930.95
19_L28_A1.2680.94
87_A93_E1.2660.94
42_V69_T1.2260.93
47_A53_I1.1910.92
72_A83_D1.1910.92
34_P37_Q1.1810.92
88_I95_L1.1790.92
65_F78_K1.1740.92
73_R89_I1.1670.92
36_K40_L1.1410.91
70_A73_R1.1390.91
6_K41_A1.1350.90
88_I91_G1.1200.90
11_I40_L1.1200.90
37_Q40_L1.1100.89
82_I86_L1.0860.88
40_L65_F1.0850.88
50_F60_E1.0810.88
38_G42_V1.0780.88
31_E40_L1.0360.86
34_P40_L1.0170.85
42_V68_H1.0040.84
31_E36_K0.9660.82
37_Q44_N0.9630.82
37_Q65_F0.9620.81
64_L69_T0.9470.80
1_F5_L0.9440.80
77_G83_D0.9190.78
45_H64_L0.8680.74
88_I94_T0.8600.74
59_E63_E0.8530.73
39_S65_F0.8520.73
41_A53_I0.8500.73
71_D75_N0.8500.73
33_K36_K0.8420.72
55_A59_E0.8360.71
72_A77_G0.8320.71
44_N47_A0.8280.71
73_R86_L0.8260.71
68_H81_N0.8220.70
14_L17_I0.8150.70
4_N8_L0.8130.69
38_G67_E0.7890.67
49_K56_E0.7780.66
49_K96_R0.7770.66
38_G68_H0.7710.65
86_L89_I0.7710.65
65_F71_D0.7700.65
10_S44_N0.7690.65
4_N66_A0.7610.64
9_P45_H0.7520.63
67_E81_N0.7260.61
7_Q23_D0.7160.59
80_P83_D0.7150.59
12_D41_A0.7080.59
48_Q60_E0.6910.57
17_I86_L0.6910.57
11_I60_E0.6880.56
6_K44_N0.6760.55
45_H49_K0.6710.55
11_I41_A0.6520.52
30_I59_E0.6490.52
33_K65_F0.6470.52
3_D7_Q0.6470.52
21_D27_V0.6450.52
28_A84_R0.6390.51
46_L83_D0.6390.51
26_V70_A0.6390.51
49_K63_E0.6370.51
6_K12_D0.6260.50
13_H16_A0.6230.49
19_L84_R0.6190.49
35_G68_H0.6180.49
51_G57_A0.6130.48
22_A96_R0.6100.48
20_L98_K0.6070.47
5_L38_G0.6030.47
86_L90_A0.5970.46
43_Y67_E0.5970.46
38_G46_L0.5880.45
38_G66_A0.5830.45
46_L50_F0.5810.45
93_E96_R0.5760.44
35_G81_N0.5670.43
2_A78_K0.5660.43
30_I49_K0.5620.43
35_G79_H0.5580.42
73_R98_K0.5560.42
61_G67_E0.5550.42
47_A52_A0.5540.42
11_I17_I0.5540.42
48_Q87_A0.5540.42
7_Q70_A0.5500.41
40_L87_A0.5500.41
46_L67_E0.5470.41
13_H45_H0.5420.40
1_F38_G0.5390.40
16_A45_H0.5380.40
32_N76_P0.5380.40
66_A71_D0.5350.40
56_E59_E0.5350.40
55_A89_I0.5290.39
60_E63_E0.5290.39
19_L97_V0.5280.39
31_E65_F0.5200.38
28_A32_N0.5200.38
88_I93_E0.5190.38
33_K51_G0.5180.38
17_I47_A0.5150.38
42_V65_F0.5110.37
22_A90_A0.5100.37
12_D44_N0.5060.37
52_A94_T0.5050.36
27_V63_E0.5050.36
3_D60_E0.5030.36
13_H44_N0.5010.36
52_A56_E0.5010.36
59_E89_I0.5000.36
20_L96_R0.5000.36
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2qhqA 2 0.9184 75.8 0.884 Contact Map
3j3bC 1 1 9.8 0.931 Contact Map
3lraA 1 0.4694 9.3 0.932 Contact Map
4pkfA 2 0.7857 8.5 0.933 Contact Map
1r9dA 2 0.8265 7.9 0.934 Contact Map
2du3A 4 0.898 7.4 0.934 Contact Map
1s4cA 2 0.5612 6.9 0.935 Contact Map
2f3oA 3 0.8571 6.9 0.935 Contact Map
1h16A 2 0.9184 6.9 0.935 Contact Map
1dmgA 1 0.6633 6.7 0.936 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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