GREMLIN Database
DUF2321 - Uncharacterized protein conserved in bacteria (DUF2321)
PFAM: PF10083 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 156 (154)
Sequences: 569 (539)
Seq/√Len: 43.4
META: 0.909

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
15_N25_N5.6581.00
91_D105_K4.9471.00
3_D19_E2.7241.00
80_E84_E2.6881.00
34_A81_R2.6521.00
26_Q30_S2.6221.00
26_Q48_R2.5801.00
122_A155_A2.5651.00
5_A13_V2.5201.00
13_V65_P2.4551.00
125_F129_L2.4031.00
27_K71_N2.2471.00
88_E117_K2.2021.00
48_R70_H2.1170.99
42_N74_K2.0460.99
87_R109_P1.9890.99
146_D149_S1.9300.99
83_L108_L1.9190.99
122_A152_A1.8940.99
152_A155_A1.8730.99
110_D121_A1.8080.98
3_D15_N1.7960.98
6_Q36_T1.7890.98
92_E124_R1.7880.98
7_I65_P1.7290.98
90_A101_R1.7050.98
9_L37_I1.5810.96
101_R104_L1.5520.96
102_E106_E1.5350.95
7_I13_V1.5270.95
150_E153_K1.5170.95
92_E107_S1.5060.95
141_R146_D1.4880.95
90_A93_L1.4870.95
100_E131_K1.4460.94
37_I41_P1.4430.94
42_N80_E1.4370.93
4_T19_E1.4310.93
15_N19_E1.4200.93
27_K35_K1.4170.93
135_E138_E1.4010.93
138_E142_D1.3800.92
5_A63_Y1.3420.91
141_R145_V1.3150.90
119_E123_A1.3090.90
92_E127_K1.3050.90
13_V63_Y1.2980.89
63_Y66_P1.2710.88
111_L125_F1.2670.88
8_C11_G1.2460.87
15_N18_A1.2460.87
18_A25_N1.2110.86
84_E117_K1.2040.85
110_D117_K1.1980.85
144_L148_A1.1900.85
135_E141_R1.1640.83
149_S154_K1.1390.82
76_Y123_A1.1340.82
86_A120_V1.1310.82
86_A89_L1.1290.82
81_R85_A1.1270.81
69_C78_W1.1260.81
8_C12_H1.1150.81
85_A104_L1.1020.80
129_L132_A1.1010.80
6_Q38_T1.0990.80
68_F140_L1.0980.80
143_I147_V1.0940.79
137_A141_R1.0830.79
85_A120_V1.0670.78
6_Q26_Q1.0540.77
89_L120_V1.0530.77
142_D145_V1.0310.75
151_A155_A1.0290.75
128_L132_A1.0040.73
82_K114_D0.9940.73
9_L35_K0.9940.73
6_Q14_I0.9630.70
47_I63_Y0.9580.70
19_E46_P0.9560.70
83_L87_R0.9550.69
92_E120_V0.9510.69
133_G137_A0.9500.69
137_A142_D0.9450.69
110_D113_K0.9440.69
148_A152_A0.9410.68
70_H76_Y0.9190.66
43_C78_W0.9170.66
9_L34_A0.9140.66
114_D149_S0.8940.64
90_A128_L0.8900.64
16_S63_Y0.8790.63
43_C72_C0.8720.62
111_L148_A0.8660.62
47_I66_P0.8630.62
68_F109_P0.8620.61
137_A140_L0.8540.61
72_C78_W0.8450.60
50_Y53_V0.8410.60
10_N116_P0.8360.59
135_E142_D0.8360.59
118_T152_A0.8350.59
7_I63_Y0.8330.59
26_Q70_H0.8300.58
18_A38_T0.8290.58
139_A143_I0.8250.58
131_K140_L0.8150.57
43_C69_C0.8090.57
114_D152_A0.8060.56
47_I76_Y0.8010.56
143_I149_S0.7970.55
3_D52_H0.7960.55
83_L86_A0.7950.55
98_E101_R0.7900.55
39_A46_P0.7770.54
117_K120_V0.7760.53
105_K125_F0.7750.53
86_A140_L0.7610.52
82_K107_S0.7610.52
15_N22_P0.7590.52
122_A140_L0.7530.51
87_R104_L0.7530.51
123_A127_K0.7510.51
84_E109_P0.7500.51
146_D150_E0.7480.51
36_T79_T0.7470.51
121_A125_F0.7470.51
61_G64_E0.7450.50
54_P68_F0.7430.50
118_T123_A0.7430.50
97_S100_E0.7420.50
53_V58_G0.7380.50
137_A145_V0.7380.50
69_C72_C0.7340.49
41_P50_Y0.7320.49
39_A59_F0.7250.49
14_I23_E0.7230.48
9_L14_I0.7130.47
4_T22_P0.7100.47
114_D118_T0.7080.47
116_P121_A0.6990.46
119_E122_A0.6960.46
32_C47_I0.6960.46
3_D22_P0.6960.46
124_R127_K0.6950.46
3_D6_Q0.6930.46
60_G64_E0.6910.45
75_P97_S0.6850.45
133_G152_A0.6780.44
74_K80_E0.6760.44
14_I36_T0.6700.43
129_L133_G0.6570.42
82_K146_D0.6560.42
29_C33_G0.6560.42
2_Y48_R0.6490.41
146_D151_A0.6450.41
125_F148_A0.6380.40
7_I16_S0.6350.40
41_P77_P0.6350.40
26_Q36_T0.6330.40
115_T121_A0.6260.39
29_C32_C0.6240.39
6_Q27_K0.6210.39
79_T111_L0.6190.39
65_P80_E0.6150.38
53_V57_I0.6140.38
90_A111_L0.6110.38
87_R115_T0.6100.38
40_C43_C0.6080.38
111_L121_A0.6060.37
96_L128_L0.6040.37
45_A55_G0.6000.37
19_E45_A0.5990.37
51_Y56_V0.5970.37
129_L138_E0.5940.36
104_L140_L0.5900.36
93_L136_A0.5880.36
30_S70_H0.5880.36
111_L155_A0.5870.36
5_A65_P0.5870.36
51_Y147_V0.5850.36
40_C72_C0.5820.35
88_E118_T0.5820.35
88_E152_A0.5790.35
40_C78_W0.5780.35
104_L125_F0.5770.35
5_A8_C0.5750.35
6_Q15_N0.5720.34
6_Q22_P0.5690.34
139_A142_D0.5620.34
45_A70_H0.5600.33
3_D25_N0.5590.33
43_C73_G0.5570.33
71_N96_L0.5550.33
129_L137_A0.5550.33
2_Y5_A0.5550.33
97_S109_P0.5530.33
45_A76_Y0.5510.33
10_N81_R0.5490.33
9_L41_P0.5440.32
103_E106_E0.5440.32
51_Y60_G0.5430.32
21_Y24_F0.5420.32
44_N148_A0.5410.32
128_L136_A0.5380.32
42_N106_E0.5350.31
54_P59_F0.5340.31
134_K146_D0.5330.31
32_C40_C0.5320.31
44_N53_V0.5300.31
56_V59_F0.5280.31
86_A121_A0.5260.31
147_V151_A0.5260.31
110_D124_R0.5250.31
136_A139_A0.5230.30
18_A26_Q0.5220.30
140_L144_L0.5220.30
73_G78_W0.5200.30
48_R53_V0.5190.30
8_C29_C0.5180.30
19_E25_N0.5170.30
139_A145_V0.5150.30
135_E147_V0.5140.30
100_E128_L0.5110.29
93_L110_D0.5100.29
88_E102_E0.5080.29
49_G66_P0.5070.29
82_K143_I0.5010.29
28_F122_A0.5010.29
39_A75_P0.5000.29
111_L139_A0.5000.29
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3j20Y 1 0.2821 76.8 0.919 Contact Map
2c2lA 1 0.4551 75.7 0.92 Contact Map
2dulA 2 0.8782 69.3 0.924 Contact Map
3o9xA 2 0.7564 65.3 0.926 Contact Map
1oahA 2 0.9744 61.9 0.927 Contact Map
4kblA 2 0.8526 61.6 0.927 Contact Map
3h0gL 1 0.25 54.6 0.93 Contact Map
3ga8A 1 0.3526 53.7 0.931 Contact Map
2f42A 2 0.4423 49.8 0.932 Contact Map
3ja82 1 0.8654 48.2 0.933 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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