GREMLIN Database
DUF2314 - Uncharacterized protein conserved in bacteria (DUF2314)
PFAM: PF10077 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 136 (121)
Sequences: 3190 (2490)
Seq/√Len: 226.4
META: 0.925

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
56_H97_S4.5231.00
110_G113_I2.7421.00
59_L71_G2.3221.00
68_R90_T2.3101.00
117_R129_D2.2241.00
42_F57_M2.1981.00
41_P54_V2.1221.00
9_A13_A2.0641.00
3_E118_K2.0181.00
8_I97_S1.9061.00
92_P95_D1.8671.00
70_S90_T1.8551.00
3_E119_R1.8091.00
57_M73_L1.7741.00
42_F89_V1.7651.00
122_P126_A1.7371.00
76_E85_L1.6971.00
19_W69_F1.6731.00
10_K13_A1.6481.00
117_R125_R1.5621.00
19_W23_A1.5361.00
14_T111_Y1.4801.00
10_K14_T1.4651.00
40_V91_V1.4391.00
44_D82_G1.4371.00
22_L62_V1.4301.00
125_R129_D1.4231.00
59_L89_V1.4151.00
72_T88_R1.3901.00
14_T17_E1.3861.00
8_I112_T1.3851.00
21_A101_Y1.3741.00
63_E70_S1.3731.00
100_M107_L1.3721.00
70_S88_R1.3701.00
16_P93_E1.3471.00
115_V119_R1.3361.00
33_E36_F1.3291.00
126_A130_K1.3231.00
60_S74_A1.3201.00
4_M8_I1.3111.00
84_K87_D1.2881.00
107_L110_G1.2791.00
100_M110_G1.2541.00
18_F36_F1.2501.00
23_A64_F1.2351.00
44_D80_V1.2271.00
43_T54_V1.2161.00
2_P5_N1.2141.00
19_W93_E1.2061.00
6_A9_A1.1961.00
5_N8_I1.1661.00
61_D72_T1.1611.00
65_D70_S1.1511.00
7_A115_V1.1401.00
15_L96_I1.1311.00
73_L83_V1.1251.00
1_D119_R1.1071.00
4_M112_T1.0961.00
39_K75_N1.0761.00
15_L38_V1.0711.00
6_A10_K1.0681.00
37_A74_A1.0661.00
126_A129_D1.0441.00
7_A112_T1.0291.00
73_L89_V1.0191.00
44_D47_G1.0101.00
64_F69_F0.9991.00
121_P124_E0.9891.00
25_P33_E0.9821.00
122_P125_R0.9761.00
33_E103_D0.9741.00
3_E115_V0.9651.00
44_D81_K0.9521.00
101_Y108_V0.9321.00
42_F83_V0.9231.00
22_L36_F0.9151.00
15_L19_W0.9140.99
44_D83_V0.9140.99
38_V71_G0.9090.99
80_V83_V0.9060.99
25_P36_F0.8980.99
114_R118_K0.8960.99
8_I12_R0.8840.99
4_M115_V0.8670.99
21_A26_K0.8630.99
5_N9_A0.8560.99
38_V96_I0.8430.99
41_P95_D0.8370.99
10_K111_Y0.8330.99
26_K32_G0.8270.99
127_E130_K0.8220.99
21_A36_F0.8180.99
63_E88_R0.8140.99
39_K56_H0.8110.99
26_K33_E0.8100.99
20_A24_A0.8050.99
25_P32_G0.8010.99
15_L93_E0.7980.99
4_M97_S0.7980.99
59_L73_L0.7890.99
57_M75_N0.7880.99
40_V95_D0.7810.98
123_E127_E0.7760.98
83_V87_D0.7700.98
40_V59_L0.7650.98
17_E21_A0.7550.98
99_W111_Y0.7540.98
69_F93_E0.7440.98
107_L114_R0.7410.98
77_P80_V0.7380.98
103_D108_V0.7340.98
21_A108_V0.7320.98
19_W22_L0.7230.97
40_V89_V0.7180.97
125_R128_F0.7170.97
2_P6_A0.7110.97
22_L25_P0.7110.97
19_W38_V0.7080.97
8_I56_H0.7030.97
19_W64_F0.6980.97
36_F101_Y0.6960.97
17_E20_A0.6930.97
68_R92_P0.6850.96
37_A60_S0.6850.96
18_F99_W0.6830.96
36_F108_V0.6820.96
22_L61_D0.6780.96
74_A85_L0.6720.96
45_G81_K0.6710.96
55_E75_N0.6700.96
42_F73_L0.6700.96
39_K112_T0.6690.96
35_G101_Y0.6690.96
65_D68_R0.6660.96
21_A33_E0.6650.96
16_P20_A0.6640.96
26_K103_D0.6610.96
64_F67_G0.6550.95
57_M77_P0.6550.95
121_P125_R0.6540.95
40_V96_I0.6490.95
44_D57_M0.6470.95
21_A25_P0.6410.95
59_L91_V0.6350.95
124_E127_E0.6350.95
13_A16_P0.6250.94
111_Y114_R0.6230.94
55_E77_P0.5990.93
57_M79_D0.5920.92
41_P97_S0.5920.92
17_E101_Y0.5910.92
15_L69_F0.5900.92
97_S112_T0.5860.92
39_K58_W0.5760.91
40_V80_V0.5740.91
105_G109_G0.5720.91
57_M80_V0.5570.90
37_A107_L0.5570.90
38_V91_V0.5550.90
112_T115_V0.5500.89
4_M7_A0.5480.89
73_L77_P0.5470.89
115_V118_K0.5440.89
76_E84_K0.5410.88
34_E105_G0.5410.88
22_L64_F0.5370.88
25_P62_V0.5350.88
114_R120_M0.5350.88
118_K125_R0.5350.88
44_D48_G0.5320.88
66_G90_T0.5300.87
32_G36_F0.5270.87
64_F68_R0.5210.86
15_L64_F0.5180.86
57_M81_K0.5160.86
55_E73_L0.5120.86
48_G54_V0.5110.85
37_A100_M0.5070.85
12_R58_W0.5060.85
116_L120_M0.5020.84
37_A102_V0.5000.84
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1c05A 1 0.8676 33.4 0.907 Contact Map
2vxaA 5 0.3015 23 0.914 Contact Map
3bbnD 1 0.8309 22.6 0.914 Contact Map
2ux9A 5 0.3162 21.2 0.915 Contact Map
2yizA 5 0.3088 20 0.916 Contact Map
2cc6A 5 0.3015 18.9 0.918 Contact Map
4tp8D 1 0.8382 16.7 0.919 Contact Map
3onrA 5 0.3382 15.9 0.92 Contact Map
4b2kA 5 0.3015 15.3 0.921 Contact Map
4b2hA 5 0.2868 14.7 0.922 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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