GREMLIN Database
DUF2312 - Uncharacterized protein conserved in bacteria (DUF2312)
PFAM: PF10073 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 72 (71)
Sequences: 7540 (3919)
Seq/√Len: 465.1
META: 0.934

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
4_Q36_N2.7141.00
45_K48_R2.3101.00
19_K23_A2.2391.00
30_Y44_R2.2101.00
49_L68_L2.2041.00
52_M60_E2.0701.00
33_A43_L2.0291.00
19_K26_I2.0081.00
13_E50_R1.9131.00
54_P58_Q1.9081.00
45_K60_E1.6771.00
12_I26_I1.4991.00
12_I30_Y1.4841.00
34_K40_V1.4661.00
16_E26_I1.4531.00
16_E20_K1.3961.00
16_E51_K1.3291.00
15_L22_I1.2921.00
58_Q62_A1.2691.00
27_K31_A1.2581.00
30_Y47_I1.2191.00
65_D69_A1.2131.00
61_E68_L1.2081.00
52_M57_R1.1961.00
52_M56_E1.1731.00
17_E21_A1.1681.00
20_K24_D1.1661.00
11_R29_V1.1611.00
21_A25_D1.1411.00
55_D58_Q1.1231.00
42_I63_L1.1201.00
39_D42_I1.1051.00
53_D56_E1.0931.00
42_I64_L1.0771.00
49_L64_L1.0741.00
8_F36_N1.0421.00
8_F43_L1.0301.00
45_K64_L1.0231.00
16_E19_K1.0181.00
15_L29_V1.0091.00
18_E22_I1.0061.00
9_I43_L1.0021.00
49_L61_E0.9871.00
46_I68_L0.9691.00
48_R52_M0.9691.00
62_A66_L0.9421.00
8_F33_A0.9341.00
48_R51_K0.9181.00
12_I47_I0.9151.00
24_D27_K0.9051.00
6_R10_E0.9041.00
23_A26_I0.9041.00
20_K23_A0.8951.00
11_R32_E0.8941.00
15_L26_I0.8881.00
20_K51_K0.8871.00
42_I60_E0.8521.00
41_K60_E0.8461.00
49_L57_R0.8401.00
4_Q7_S0.8341.00
30_Y43_L0.8061.00
46_I64_L0.8031.00
22_I25_D0.7961.00
14_R17_E0.7951.00
19_K22_I0.7851.00
42_I45_K0.7801.00
57_R61_E0.7791.00
24_D28_E0.7541.00
60_E64_L0.7451.00
14_R18_E0.7401.00
45_K49_L0.7271.00
7_S11_R0.7161.00
5_L38_F0.7151.00
18_E25_D0.7091.00
18_E21_A0.6931.00
31_A35_G0.6911.00
2_A38_F0.6881.00
64_L68_L0.6791.00
29_V32_E0.6611.00
65_D68_L0.6541.00
15_L18_E0.6541.00
4_Q8_F0.6421.00
9_I47_I0.6341.00
66_L69_A0.5960.99
57_R68_L0.5960.99
11_R25_D0.5940.99
49_L52_M0.5920.99
8_F32_E0.5890.99
28_E31_A0.5880.99
8_F11_R0.5860.99
10_E14_R0.5840.99
26_I30_Y0.5790.99
62_A65_D0.5790.99
13_E51_K0.5750.99
60_E63_L0.5730.99
3_D6_R0.5660.99
14_R25_D0.5650.99
63_L66_L0.5590.99
12_I29_V0.5580.99
4_Q38_F0.5540.99
54_P57_R0.5480.99
15_L25_D0.5470.99
3_D7_S0.5300.98
5_L43_L0.5180.98
68_L72_G0.5040.98
16_E47_I0.5000.97
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4ut1A 1 0.7083 17.7 0.831 Contact Map
1gaxA 1 1 17.2 0.832 Contact Map
3kxeC 2 0.6944 12.5 0.841 Contact Map
1i84S 2 0 12.1 0.842 Contact Map
4rsiA 1 0.7361 10.5 0.847 Contact Map
2zdiC 2 0.5833 9.5 0.85 Contact Map
2d4yA 1 0.6806 9.4 0.851 Contact Map
2ma3A 1 0.9167 8.7 0.852 Contact Map
2q7tA 1 0.5139 8.4 0.854 Contact Map
4abxA 2 0.9167 7.2 0.857 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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