GREMLIN Database
DUF2303 - Uncharacterized conserved protein (DUF2303)
PFAM: PF10065 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 269 (249)
Sequences: 1223 (1103)
Seq/√Len: 69.9
META: 0.742

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
91_D102_D8.9461.00
57_L105_A4.3911.00
121_I235_Y3.8891.00
50_R102_D3.8451.00
220_A237_L3.4941.00
78_F92_Y3.4091.00
149_D152_D3.1451.00
79_A248_F3.0611.00
127_S130_E2.9291.00
117_A138_N2.9031.00
72_D99_G2.6241.00
69_R76_V2.5781.00
51_K70_H2.4421.00
125_K234_W2.4191.00
178_E187_H2.3481.00
54_T104_R2.2841.00
138_N141_D2.2651.00
202_E219_T2.2431.00
66_Y105_A2.1781.00
92_Y101_G2.1671.00
120_A124_K2.1601.00
32_V39_E2.1361.00
68_N71_K2.1271.00
115_W240_P2.1091.00
87_T104_R2.0921.00
42_E52_R2.0741.00
77_I264_V2.0251.00
42_E45_L1.9581.00
218_I243_V1.9151.00
34_E37_K1.9151.00
67_V88_A1.8951.00
70_H103_H1.8801.00
86_A255_I1.8271.00
53_G91_D1.8011.00
90_L105_A1.7901.00
63_F86_A1.7831.00
114_E141_D1.7711.00
203_F222_L1.7471.00
77_I88_A1.7231.00
61_D65_A1.7061.00
142_I153_L1.7051.00
121_I126_M1.6981.00
112_S208_P1.6841.00
78_F101_G1.6841.00
216_Y246_E1.6290.99
204_T217_R1.6080.99
54_T106_T1.5810.99
50_R91_D1.5810.99
70_H92_Y1.5700.99
206_G216_Y1.5610.99
134_F138_N1.5550.99
151_A155_E1.5410.99
216_Y250_E1.5150.99
211_E245_E1.5050.99
112_S209_P1.4460.99
180_Q196_K1.4400.99
135_L153_L1.4390.99
53_G99_G1.4370.98
87_T106_T1.4330.98
66_Y90_L1.4290.98
116_K120_A1.4270.98
164_R185_E1.4190.98
205_L218_I1.3970.98
109_L251_F1.3890.98
76_V92_Y1.3880.98
136_E221_R1.3860.98
74_G94_T1.3760.98
45_L49_R1.3730.98
216_Y247_A1.3660.98
133_E236_R1.3640.98
76_V265_F1.3200.97
67_V105_A1.3130.97
253_E257_E1.3100.97
59_D65_A1.3030.97
131_F235_Y1.2900.97
53_G70_H1.2680.97
67_V75_S1.2640.97
137_D236_R1.2390.96
206_G217_R1.2380.96
80_D83_A1.2290.96
91_D103_H1.2170.96
42_E46_P1.2100.96
206_G215_P1.2080.96
169_K184_S1.2040.95
168_F231_L1.2010.95
204_T219_T1.1950.95
60_L255_I1.1660.95
121_I124_K1.1620.95
159_A199_I1.1610.94
153_L157_A1.1460.94
91_D99_G1.1280.94
175_D201_E1.1230.93
66_Y103_H1.1180.93
56_T106_T1.1110.93
254_K259_E1.1010.93
181_F264_V1.0920.92
51_K99_G1.0860.92
147_D204_T1.0700.92
50_R100_W1.0680.92
118_W141_D1.0650.91
77_I262_L1.0630.91
52_R102_D1.0610.91
30_V38_L1.0500.91
246_E250_E1.0430.91
57_L66_Y1.0380.90
220_A235_Y1.0380.90
59_D62_S1.0360.90
161_Q169_K1.0350.90
113_A116_K1.0320.90
111_L116_K1.0260.90
51_K92_Y1.0240.90
112_S141_D1.0190.90
225_R234_W1.0120.89
173_R201_E1.0120.89
133_E221_R1.0020.89
72_D90_L1.0010.89
49_R52_R0.9920.88
53_G66_Y0.9910.88
166_V185_E0.9780.88
222_L233_L0.9700.87
39_E43_K0.9700.87
77_I255_I0.9680.87
164_R226_I0.9610.87
84_M210_F0.9540.86
119_L122_N0.9470.86
242_K245_E0.9420.86
99_G102_D0.9360.85
33_P36_Y0.9340.85
48_P100_W0.9330.85
66_Y70_H0.9280.85
93_H98_P0.9230.84
115_W209_P0.9210.84
28_P49_R0.9210.84
126_M234_W0.9210.84
111_L119_L0.9190.84
90_L99_G0.9180.84
63_F255_I0.9110.84
31_V36_Y0.9110.84
79_A86_A0.9080.83
162_A187_H0.9040.83
114_E242_K0.9030.83
53_G92_Y0.9000.83
35_G39_E0.8880.82
51_K98_P0.8740.81
226_I231_L0.8660.80
159_A191_A0.8650.80
84_M109_L0.8640.80
65_A69_R0.8630.80
63_F77_I0.8620.80
85_S108_A0.8610.80
77_I90_L0.8610.80
128_Q170_S0.8600.80
75_S90_L0.8550.80
40_D44_Y0.8500.79
73_E95_A0.8490.79
245_E249_Q0.8440.79
63_F67_V0.8410.78
32_V35_G0.8360.78
163_T166_V0.8360.78
61_D254_K0.8340.78
237_L243_V0.8310.78
179_V182_S0.8280.77
74_G99_G0.8240.77
77_I260_T0.8210.77
84_M248_F0.8210.77
82_D110_P0.8150.76
70_H76_V0.8110.76
141_D205_L0.8090.76
227_G232_T0.8090.76
252_V264_V0.8090.76
63_F88_A0.8080.76
122_N237_L0.8080.76
113_A117_A0.8070.75
124_K130_E0.8030.75
111_L244_L0.8020.75
55_V66_Y0.7920.74
156_L197_L0.7900.74
162_A166_V0.7880.74
237_L240_P0.7870.74
42_E49_R0.7840.73
115_W136_E0.7810.73
100_W139_A0.7800.73
107_L236_R0.7780.73
80_D268_T0.7740.72
21_I24_D0.7650.72
122_N235_Y0.7620.71
123_G236_R0.7580.71
141_D203_F0.7580.71
114_E140_P0.7560.71
247_A251_F0.7540.70
26_G29_L0.7500.70
93_H101_G0.7450.70
252_V256_A0.7420.69
253_E256_A0.7390.69
165_K186_E0.7350.68
118_W205_L0.7320.68
68_N260_T0.7310.68
250_E253_E0.7310.68
56_T268_T0.7270.68
139_A154_L0.7240.67
168_F233_L0.7190.67
212_G245_E0.7170.67
28_P45_L0.7160.66
60_L251_F0.7140.66
140_P239_R0.7130.66
79_A252_V0.7120.66
18_A21_I0.7120.66
166_V226_I0.7090.66
242_K255_I0.7080.66
214_E254_K0.7060.65
132_A239_R0.7050.65
20_I29_L0.7030.65
58_T110_P0.7000.65
221_R238_V0.7000.65
51_K94_T0.6980.64
113_A245_E0.6960.64
114_E138_N0.6950.64
227_G230_K0.6950.64
250_E254_K0.6910.64
125_K130_E0.6900.64
59_D212_G0.6880.63
125_K232_T0.6880.63
149_D204_T0.6870.63
88_A105_A0.6870.63
38_L41_L0.6850.63
28_P31_V0.6830.63
177_G225_R0.6790.62
71_K75_S0.6790.62
161_Q173_R0.6780.62
66_Y91_D0.6770.62
220_A234_W0.6720.62
25_T44_Y0.6710.61
22_E25_T0.6690.61
199_I203_F0.6660.61
203_F235_Y0.6640.61
51_K93_H0.6640.61
220_A233_L0.6640.61
72_D75_S0.6600.60
156_L201_E0.6550.60
122_N240_P0.6550.60
107_L111_L0.6530.59
16_A20_I0.6520.59
209_P243_V0.6500.59
107_L212_G0.6500.59
126_M134_F0.6490.59
53_G102_D0.6480.59
213_G216_Y0.6470.59
174_L180_Q0.6470.59
79_A210_F0.6440.58
137_D221_R0.6430.58
94_T98_P0.6430.58
241_E245_E0.6430.58
199_I222_L0.6420.58
40_D49_R0.6410.58
28_P40_D0.6400.58
32_V36_Y0.6340.57
27_T40_D0.6340.57
210_F244_L0.6340.57
70_H98_P0.6300.57
68_N259_E0.6300.57
66_Y128_Q0.6300.57
114_E208_P0.6260.56
83_A87_T0.6240.56
50_R53_G0.6220.56
249_Q253_E0.6220.56
209_P247_A0.6210.56
68_N97_A0.6210.56
248_F251_F0.6200.55
56_T108_A0.6190.55
181_F231_L0.6190.55
89_V265_F0.6180.55
174_L224_Y0.6170.55
206_G218_I0.6130.55
18_A22_E0.6120.54
16_A21_I0.6110.54
47_A98_P0.6090.54
67_V71_K0.6080.54
166_V183_Y0.6080.54
36_Y39_E0.6070.54
261_G268_T0.6060.54
177_G224_Y0.6040.54
87_T108_A0.6020.53
173_R176_S0.6010.53
132_A136_E0.5990.53
88_A264_V0.5980.53
157_A233_L0.5980.53
85_S106_T0.5920.52
248_F252_V0.5910.52
24_D27_T0.5910.52
116_K186_E0.5910.52
114_E239_R0.5900.52
126_M131_F0.5900.52
119_L209_P0.5890.52
164_R187_H0.5840.51
17_A21_I0.5820.51
63_F132_A0.5820.51
187_H192_G0.5770.50
152_D211_E0.5770.50
117_A134_F0.5760.50
191_A200_P0.5750.50
195_G205_L0.5740.50
167_T230_K0.5710.50
115_W122_N0.5710.50
114_E241_E0.5660.49
181_F255_I0.5650.49
168_F226_I0.5640.49
45_L130_E0.5630.49
114_E127_S0.5620.48
58_T80_D0.5610.48
146_F149_D0.5590.48
90_L98_P0.5580.48
30_V34_E0.5560.48
18_A30_V0.5550.48
205_L208_P0.5530.47
115_W119_L0.5530.47
17_A22_E0.5520.47
64_I68_N0.5520.47
75_S91_D0.5520.47
169_K194_K0.5510.47
81_A211_E0.5490.47
126_M235_Y0.5480.47
26_G98_P0.5470.47
61_D261_G0.5460.46
115_W135_L0.5460.46
189_A233_L0.5460.46
51_K91_D0.5450.46
214_E250_E0.5450.46
187_H191_A0.5410.46
172_V180_Q0.5410.46
127_S232_T0.5410.46
107_L110_P0.5390.46
88_A203_F0.5390.46
64_I255_I0.5380.46
181_F230_K0.5370.45
37_K43_K0.5330.45
23_L27_T0.5320.45
224_Y229_G0.5310.45
218_I240_P0.5300.45
17_A20_I0.5300.45
94_T99_G0.5290.44
89_V101_G0.5290.44
182_S231_L0.5270.44
64_I243_V0.5270.44
157_A160_L0.5270.44
92_Y99_G0.5250.44
159_A162_A0.5250.44
207_I218_I0.5240.44
160_L233_L0.5240.44
69_R73_E0.5230.44
135_L203_F0.5230.44
22_E37_K0.5220.44
53_G104_R0.5220.44
143_V206_G0.5210.43
133_E234_W0.5210.43
245_E248_F0.5200.43
155_E158_R0.5200.43
167_T227_G0.5190.43
147_D254_K0.5190.43
96_D152_D0.5170.43
251_F254_K0.5150.43
59_D247_A0.5140.43
162_A168_F0.5130.43
199_I224_Y0.5120.42
118_W148_P0.5080.42
115_W207_I0.5070.42
20_I23_L0.5070.42
64_I259_E0.5050.42
18_A24_D0.5040.41
57_L137_D0.5030.41
64_I260_T0.5030.41
16_A24_D0.5020.41
199_I226_I0.5000.41
23_L29_L0.5000.41
171_A230_K0.5000.41
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3jrxA 1 0.1896 8.1 0.964 Contact Map
2m71A 1 0.2751 7.7 0.965 Contact Map
2qezA 3 0.3086 7.7 0.965 Contact Map
3abqA 3 0.3048 7.5 0.965 Contact Map
4bp8A 1 0.2937 6.2 0.966 Contact Map
1tigA 1 0.2677 5.4 0.967 Contact Map
2ifeA 1 0.2714 4.7 0.968 Contact Map
1i96V 1 0.2639 4.5 0.968 Contact Map
1o7dA 1 0.2193 4 0.969 Contact Map
3tysA 2 0.2379 4 0.969 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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