GREMLIN Database
DUF2301 - Uncharacterized integral membrane protein (DUF2301)
PFAM: PF10063 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 135 (135)
Sequences: 532 (344)
Seq/√Len: 29.6
META: 0.682

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
48_L51_L5.5111.00
15_R18_Q3.9131.00
20_F58_A3.8621.00
33_L39_L3.4891.00
1_L5_T2.7681.00
27_G31_L2.6181.00
12_P16_A2.5810.99
75_E117_R2.5550.99
127_I130_K2.5290.99
52_G55_P2.5260.99
6_I63_I2.3280.99
119_F125_P2.2830.99
68_A72_N2.2700.99
42_T46_Q2.1670.98
94_L109_V2.1460.98
120_F125_P2.1450.98
65_F80_T1.9930.97
72_N77_K1.9020.96
79_L115_A1.8860.96
37_E41_L1.8490.95
11_R15_R1.8070.94
122_P125_P1.8050.94
50_L58_A1.7960.94
8_I17_L1.6940.92
114_F121_Q1.6470.91
68_A77_K1.5680.89
99_V106_L1.5480.88
102_V105_G1.5330.88
3_L8_I1.5260.88
107_W111_F1.3560.80
25_C28_S1.3510.80
14_H17_L1.3330.79
2_S74_L1.3220.79
14_H18_Q1.3020.78
126_D129_D1.3020.78
51_L58_A1.2900.77
22_L90_H1.2580.75
4_F134_A1.2570.75
116_L120_F1.2460.74
27_G55_P1.2390.74
14_H71_F1.2270.73
1_L4_F1.2030.72
106_L109_V1.1920.71
98_E102_V1.1900.71
54_G62_G1.1870.71
30_A92_S1.1720.70
1_L42_T1.1500.68
29_L92_S1.1330.67
63_I134_A1.1270.67
34_S39_L1.1270.67
128_G131_S1.1100.65
87_L104_L1.0910.64
23_I36_P1.0840.64
102_V106_L1.0610.62
4_F51_L1.0440.61
16_A20_F1.0440.61
29_L115_A1.0430.61
99_V102_V1.0430.61
66_K70_C1.0330.60
42_T95_L1.0140.58
86_L100_E1.0090.58
97_V105_G1.0040.58
35_G39_L1.0020.57
54_G58_A1.0020.57
19_L98_E1.0010.57
72_N119_F0.9970.57
23_I31_L0.9860.56
17_L69_F0.9640.54
107_W114_F0.9610.54
23_I99_V0.9580.54
29_L102_V0.9310.52
108_A124_P0.9290.52
10_L13_L0.9260.51
46_Q53_V0.9230.51
34_S42_T0.9130.50
45_N112_L0.9070.50
17_L60_L0.9000.49
47_P63_I0.8930.49
5_T16_A0.8840.48
68_A114_F0.8750.47
28_S32_A0.8720.47
67_E70_C0.8660.47
117_R131_S0.8620.46
37_E43_V0.8460.45
2_S5_T0.8430.45
48_L105_G0.8400.45
54_G59_A0.8390.45
106_L112_L0.8390.45
78_L112_L0.8290.44
108_A116_L0.8270.44
33_L41_L0.8220.43
109_V116_L0.8120.43
34_S92_S0.8100.42
54_G63_I0.8050.42
78_L106_L0.8050.42
75_E84_P0.8030.42
2_S68_A0.8020.42
92_S110_L0.8020.42
46_Q92_S0.8000.42
25_C92_S0.7890.41
85_L109_V0.7880.41
28_S31_L0.7820.40
88_L91_L0.7750.40
51_L92_S0.7650.39
90_H100_E0.7600.39
13_L17_L0.7590.39
35_G42_T0.7580.38
11_R14_H0.7480.38
59_A83_V0.7480.38
25_C56_L0.7440.37
64_G83_V0.7320.37
8_I21_W0.7220.36
73_R76_T0.7210.36
64_G81_P0.7190.36
65_F76_T0.7190.36
106_L122_P0.7060.35
45_N106_L0.6940.34
25_C82_L0.6940.34
53_V58_A0.6860.33
45_N51_L0.6840.33
76_T127_I0.6790.33
40_A74_L0.6780.33
37_E115_A0.6750.32
36_P48_L0.6710.32
24_G62_G0.6700.32
68_A119_F0.6690.32
45_N92_S0.6680.32
79_L83_V0.6650.32
68_A78_L0.6630.32
32_A35_G0.6550.31
14_H73_R0.6500.31
93_G109_V0.6480.31
8_I80_T0.6460.31
3_L21_W0.6410.30
119_F123_I0.6380.30
51_L95_L0.6320.30
6_I9_Y0.6290.29
78_L122_P0.6220.29
3_L13_L0.6180.29
8_I13_L0.6160.29
14_H21_W0.6030.28
19_L30_A0.5980.27
124_P127_I0.5950.27
40_A97_V0.5950.27
44_Y96_P0.5920.27
8_I57_F0.5900.27
116_L125_P0.5890.27
30_A81_P0.5870.27
22_L106_L0.5810.26
30_A127_I0.5810.26
13_L21_W0.5810.26
34_S116_L0.5790.26
118_K126_D0.5770.26
71_F135_Y0.5770.26
15_R43_V0.5760.26
30_A106_L0.5760.26
36_P39_L0.5750.26
24_G89_G0.5700.26
26_I45_N0.5680.26
109_V122_P0.5670.25
110_L122_P0.5660.25
65_F75_E0.5610.25
46_Q81_P0.5610.25
68_A71_F0.5590.25
97_V101_Q0.5580.25
30_A94_L0.5580.25
59_A62_G0.5530.25
125_P132_V0.5520.25
31_L110_L0.5470.24
1_L46_Q0.5470.24
92_S98_E0.5440.24
128_G133_F0.5430.24
3_L133_F0.5420.24
10_L20_F0.5410.24
15_R34_S0.5400.24
125_P133_F0.5380.24
35_G102_V0.5370.24
58_A62_G0.5340.24
31_L35_G0.5340.24
99_V120_F0.5310.23
118_K128_G0.5310.23
16_A98_E0.5310.23
31_L94_L0.5300.23
26_I122_P0.5290.23
10_L22_L0.5280.23
55_P76_T0.5270.23
132_V135_Y0.5260.23
15_R29_L0.5230.23
102_V116_L0.5200.23
73_R77_K0.5200.23
28_S61_T0.5200.23
2_S120_F0.5200.23
45_N109_V0.5190.23
67_E126_D0.5170.23
23_I33_L0.5170.23
37_E45_N0.5090.22
18_Q24_G0.5090.22
29_L33_L0.5080.22
22_L28_S0.5080.22
73_R133_F0.5060.22
30_A109_V0.5020.22
75_E131_S0.5000.22
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3rceA 1 0.9111 3 0.957 Contact Map
1floA 3 0.5556 3 0.957 Contact Map
2l6wA 2 0.2815 2.1 0.961 Contact Map
2k4jA 1 0.2889 1.9 0.962 Contact Map
4xk8G 1 0.2815 1.8 0.962 Contact Map
2hqrA 2 0.3333 1.8 0.962 Contact Map
4b09A 2 0.3333 1.6 0.963 Contact Map
2m59A 2 0.2296 1.4 0.964 Contact Map
1q90G 1 0.2222 1.2 0.966 Contact Map
2l16A 1 0.2963 1.2 0.966 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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