GREMLIN Database
DUF2294 - Uncharacterized conserved protein (DUF2294)
PFAM: PF10057 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 111 (107)
Sequences: 10220 (5651)
Seq/√Len: 546.3
META: 0.921

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
10_S30_T3.2361.00
12_A80_A3.0181.00
82_E88_K2.8771.00
74_R78_E2.8321.00
88_K110_E2.2331.00
57_E68_Q2.2061.00
51_T54_E2.1571.00
7_A11_N2.0921.00
97_S100_T2.0211.00
80_A83_R1.9411.00
27_K42_E1.9261.00
37_V109_L1.9131.00
79_A83_R1.8851.00
26_T43_G1.8511.00
60_D64_E1.7681.00
12_A83_R1.6651.00
14_V28_A1.6631.00
20_Y73_M1.6341.00
13_I77_L1.5761.00
80_A84_I1.5671.00
61_L64_E1.5501.00
75_P79_A1.4741.00
5_L84_I1.4721.00
38_V106_V1.4501.00
13_I39_V1.3971.00
61_L68_Q1.3901.00
36_L40_V1.3801.00
30_T39_V1.3741.00
32_L37_V1.3731.00
10_S28_A1.3651.00
12_A84_I1.3621.00
55_S72_A1.3121.00
9_I37_V1.3041.00
61_L65_V1.2851.00
18_K24_G1.2801.00
91_A97_S1.2771.00
47_P50_R1.2041.00
8_A12_A1.1881.00
9_I81_V1.1801.00
42_E90_R1.1701.00
15_R19_E1.1681.00
70_Q105_E1.1661.00
17_E21_T1.1531.00
5_L85_T1.1291.00
12_A15_R1.1241.00
78_E82_E1.1231.00
57_E61_L1.1191.00
37_V85_T1.1161.00
87_R110_E1.1101.00
67_R95_D1.0881.00
31_Y34_D1.0731.00
16_L77_L1.0571.00
11_N15_R1.0531.00
50_R101_G1.0411.00
96_I103_A1.0391.00
70_Q94_S1.0011.00
48_A69_L0.9981.00
8_A84_I0.9941.00
8_A11_N0.9891.00
44_V103_A0.9861.00
36_L106_V0.9851.00
93_H102_E0.9801.00
45_L53_V0.9751.00
31_Y40_V0.9671.00
76_E79_A0.9601.00
64_E68_Q0.9361.00
70_Q92_F0.9301.00
67_R71_E0.9301.00
57_E71_E0.9251.00
93_H104_V0.9251.00
75_P78_E0.9221.00
65_V68_Q0.9211.00
79_A82_E0.9181.00
9_I30_T0.9101.00
45_L96_I0.9071.00
19_E76_E0.9071.00
6_E32_L0.9021.00
93_H97_S0.8971.00
40_V104_V0.8621.00
63_K98_T0.8601.00
94_S105_E0.8591.00
104_V108_V0.8421.00
57_E60_D0.8411.00
16_L80_A0.8271.00
59_R99_D0.8121.00
71_E74_R0.8111.00
33_I38_V0.7941.00
40_V108_V0.7931.00
17_E103_A0.7811.00
33_I36_L0.7761.00
21_T69_L0.7721.00
10_S14_V0.7651.00
91_A100_T0.7611.00
24_G29_R0.7531.00
6_E31_Y0.7401.00
46_T66_R0.7401.00
20_Y76_E0.7391.00
63_K96_I0.7371.00
29_R35_D0.7271.00
13_I41_L0.7271.00
41_L44_V0.7241.00
102_E108_V0.7021.00
50_R54_E0.6971.00
10_S39_V0.6941.00
50_R53_V0.6841.00
4_E7_A0.6791.00
95_D104_V0.6771.00
62_V65_V0.6761.00
7_A10_S0.6731.00
63_K95_D0.6691.00
48_A65_V0.6601.00
89_V107_F0.6551.00
53_V62_V0.6541.00
51_T55_S0.6501.00
17_E41_L0.6491.00
16_L73_M0.6441.00
51_T76_E0.6421.00
4_E8_A0.6391.00
20_Y69_L0.6331.00
92_F105_E0.6331.00
90_R110_E0.6301.00
59_R63_K0.6151.00
57_E72_A0.6141.00
93_H96_I0.6131.00
81_V85_T0.6121.00
6_E30_T0.6081.00
29_R34_D0.6081.00
19_E47_P0.5861.00
5_L9_I0.5841.00
8_A83_R0.5820.99
77_L107_F0.5800.99
22_G25_P0.5690.99
46_T49_E0.5680.99
35_D40_V0.5640.99
94_S103_A0.5630.99
63_K71_E0.5600.99
43_G50_R0.5570.99
44_V65_V0.5530.99
18_K26_T0.5500.99
26_T42_E0.5490.99
97_S102_E0.5480.99
35_D87_R0.5450.99
78_E92_F0.5410.99
19_E88_K0.5400.99
46_T103_A0.5400.99
27_K43_G0.5380.99
42_E102_E0.5370.99
58_G62_V0.5330.99
91_A102_E0.5330.99
35_D42_E0.5330.99
63_K67_R0.5280.99
59_R68_Q0.5270.99
41_L103_A0.5250.99
82_E89_V0.5220.99
55_S68_Q0.5070.99
55_S58_G0.5060.99
59_R71_E0.5040.99
49_E62_V0.5030.99
85_T88_K0.5010.98
17_E65_V0.5000.98
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2x4kA 4 0.4414 27 0.867 Contact Map
3kanA 3 0.4505 26.6 0.867 Contact Map
2opaA 4 0.4595 23.3 0.871 Contact Map
3ej9A 3 0.4865 18.5 0.877 Contact Map
1gyxA 2 0.5495 17.9 0.877 Contact Map
3djhA 3 0.4324 15.7 0.88 Contact Map
2os5A 7 0.4685 14.5 0.882 Contact Map
1uizA 3 0.4324 14.5 0.882 Contact Map
3m21A 4 0.4775 13.6 0.884 Contact Map
3t5sA 3 0.2703 13.3 0.884 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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