GREMLIN Database
DUF2280 - Uncharacterized conserved protein (DUF2280)
PFAM: PF10045 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 103 (101)
Sequences: 976 (647)
Seq/√Len: 64.4
META: 0.746

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
68_K72_E3.8171.00
57_K61_D3.7491.00
90_Q94_E3.2241.00
30_K36_E2.9561.00
8_V29_V2.5941.00
5_T8_V2.2151.00
31_E54_N2.1431.00
10_A62_L2.0181.00
88_A93_A1.8711.00
95_K99_M1.8581.00
50_R53_R1.7761.00
29_V33_F1.7711.00
11_F28_A1.7621.00
6_D9_K1.7451.00
73_D76_D1.6820.99
15_A20_D1.6720.99
12_I25_V1.6350.99
56_A59_W1.6300.99
84_V88_A1.5740.99
30_K44_A1.5540.99
88_A97_E1.5440.99
61_D65_A1.5070.99
25_V42_V1.5020.99
43_E49_K1.4780.98
93_A97_E1.4710.98
79_I86_L1.4520.98
74_T83_A1.4150.98
2_A41_Q1.3770.97
8_V35_I1.3430.97
55_L60_R1.3130.96
69_A76_D1.2910.96
62_L66_T1.2840.96
91_R97_E1.2800.96
38_S41_Q1.2530.95
40_Q43_E1.2290.95
20_D28_A1.2280.95
1_M41_Q1.2190.95
21_T24_Q1.2050.94
45_Y56_A1.2030.94
37_V42_V1.1950.94
78_P81_N1.1900.94
26_A42_V1.1850.94
37_V41_Q1.1780.94
29_V35_I1.1750.94
19_F81_N1.1670.93
11_F33_F1.1610.93
6_D58_K1.1580.93
19_F71_L1.1530.93
60_R64_E1.1310.92
26_A39_R1.0660.90
69_A73_D1.0270.88
65_A69_A1.0190.88
48_T63_F1.0140.87
27_E31_E1.0130.87
70_F83_A1.0030.87
12_I41_Q0.9910.86
79_I84_V0.9900.86
78_P90_Q0.9820.86
29_V39_R0.9780.85
47_P55_L0.9660.85
73_D83_A0.9600.84
65_A75_A0.9530.84
14_Q66_T0.9430.83
18_C66_T0.9330.82
11_F29_V0.9110.81
52_G55_L0.9110.81
19_F60_R0.8920.80
49_K52_G0.8860.79
43_E52_G0.8660.77
20_D24_Q0.8630.77
12_I29_V0.8480.76
92_M96_A0.8170.73
20_D87_R0.8130.73
23_S27_E0.8040.72
65_A71_L0.7940.71
8_V12_I0.7810.70
16_L46_D0.7790.70
29_V37_V0.7620.68
88_A91_R0.7600.68
19_F61_D0.7540.67
61_D64_E0.7520.67
95_K98_E0.7460.66
10_A59_W0.7450.66
27_E98_E0.7420.66
96_A99_M0.7350.65
69_A84_V0.7320.65
46_D49_K0.7280.64
74_T78_P0.7180.63
83_A87_R0.7140.63
18_C70_F0.7020.62
44_A54_N0.6890.60
62_L65_A0.6890.60
43_E53_R0.6790.59
84_V90_Q0.6790.59
44_A56_A0.6760.59
79_I90_Q0.6740.58
41_Q44_A0.6740.58
79_I93_A0.6610.57
25_V28_A0.6590.57
43_E51_A0.6530.56
55_L63_F0.6470.55
92_M97_E0.6460.55
52_G63_F0.6440.55
12_I42_V0.6380.54
30_K35_I0.6160.52
57_K60_R0.6100.51
3_A41_Q0.6100.51
32_E57_K0.6100.51
16_L42_V0.6080.51
78_P83_A0.6040.50
74_T82_K0.6010.50
71_L90_Q0.5980.50
3_A44_A0.5980.50
55_L59_W0.5980.50
47_P63_F0.5920.49
25_V46_D0.5880.49
9_K41_Q0.5880.49
92_M95_K0.5840.48
96_A101_N0.5820.48
39_R42_V0.5810.48
46_D63_F0.5800.48
41_Q45_Y0.5800.48
22_P46_D0.5790.47
48_T52_G0.5780.47
7_E53_R0.5690.46
95_K100_G0.5660.46
59_W75_A0.5600.45
71_L75_A0.5600.45
7_E78_P0.5590.45
66_T70_F0.5580.45
34_G38_S0.5560.45
13_V59_W0.5460.44
26_A37_V0.5440.43
26_A40_Q0.5440.43
47_P52_G0.5360.43
6_D10_A0.5360.43
94_E97_E0.5340.42
20_D82_K0.5310.42
77_I80_A0.5270.42
44_A53_R0.5250.41
33_F39_R0.5250.41
89_L93_A0.5220.41
79_I97_E0.5200.41
12_I16_L0.5160.40
56_A98_E0.5100.40
74_T77_I0.5080.39
28_A33_F0.5060.39
65_A94_E0.5060.39
58_K64_E0.5060.39
52_G59_W0.5030.39
11_F15_A0.5020.39
2_A48_T0.5000.39
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2ao9A 7 0.8544 51.7 0.894 Contact Map
2jn6A 1 0.7864 47.9 0.896 Contact Map
1iufA 1 0.7864 43.9 0.899 Contact Map
1pdnC 1 0.4369 41.2 0.901 Contact Map
2hl7A 1 0.4078 39.7 0.901 Contact Map
1u78A 1 0.8447 38.1 0.902 Contact Map
2kw0A 1 0.4369 35.7 0.904 Contact Map
4yfkF 1 0.5922 33.5 0.905 Contact Map
1s7oA 3 0.7767 32.5 0.906 Contact Map
1k78A 1 0.4757 31.2 0.907 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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