GREMLIN Database
DUF2278 - Uncharacterized conserved protein (DUF2278)
PFAM: PF10042 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 203 (202)
Sequences: 1551 (1132)
Seq/√Len: 79.6
META: 0.79

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
58_L191_A4.1491.00
3_L142_E3.7721.00
7_G80_F3.7151.00
92_L174_G3.6131.00
80_F150_I3.5621.00
4_K142_E3.3921.00
32_I46_V3.3151.00
33_H41_D3.1631.00
44_I139_A3.0481.00
11_G34_V2.9751.00
10_K138_Y2.9001.00
161_G167_N2.8851.00
78_D136_R2.6141.00
152_D166_D2.6001.00
65_F187_A2.5811.00
143_R148_N2.5581.00
13_V131_I2.5231.00
101_D104_D2.4841.00
169_V203_G2.2521.00
77_P81_T2.1281.00
31_Q43_R2.0441.00
57_L107_P2.0421.00
164_A167_N2.0251.00
40_T148_N2.0211.00
34_V44_I1.9941.00
10_K136_R1.9481.00
36_D78_D1.9461.00
102_P197_W1.8841.00
176_L185_W1.8691.00
159_N166_D1.7931.00
44_I153_I1.7511.00
9_L34_V1.7441.00
139_A153_I1.7421.00
30_Y124_D1.6871.00
83_L90_G1.6631.00
95_I98_N1.6521.00
58_L105_M1.6491.00
35_V41_D1.6101.00
124_D128_Q1.6071.00
114_G118_D1.5841.00
15_D33_H1.5721.00
16_R30_Y1.5520.99
158_G199_T1.5330.99
118_D121_D1.5250.99
43_R144_W1.5230.99
42_Y143_R1.5040.99
135_A179_F1.5000.99
126_Y186_V1.4780.99
80_F143_R1.4620.99
7_G143_R1.4030.99
138_Y178_H1.3880.99
97_G170_W1.3360.98
85_S91_A1.3260.98
178_H183_N1.3220.98
60_L100_F1.3140.98
198_H203_G1.2860.98
160_S163_F1.2680.98
97_G174_G1.2510.98
145_G148_N1.2360.97
127_V177_I1.2320.97
160_S167_N1.2200.97
85_S89_G1.2140.97
142_E152_D1.2100.97
95_I196_S1.2030.97
85_S88_G1.1990.97
198_H201_D1.1930.97
73_L90_G1.1930.97
54_P194_S1.1730.96
179_F186_V1.1610.96
200_D203_G1.1550.96
49_K55_S1.1450.96
163_F166_D1.1420.96
178_H185_W1.1290.96
83_L91_A1.1290.96
116_D122_K1.1280.96
95_I198_H1.1240.95
106_R122_K1.1200.95
3_L171_Q1.1140.95
16_R124_D1.0940.95
49_K56_E1.0850.94
94_Y97_G1.0710.94
91_A170_W1.0590.94
32_I127_V1.0450.93
8_V81_T1.0380.93
191_A197_W1.0370.93
14_V35_V1.0300.93
80_F148_N1.0150.92
74_A138_Y1.0130.92
139_A175_L1.0020.92
161_G164_A0.9800.91
36_D42_Y0.9780.91
42_Y148_N0.9720.90
72_A90_G0.9710.90
86_K91_A0.9700.90
100_F105_M0.9690.90
192_F195_Q0.9620.90
18_R121_D0.9440.89
83_L89_G0.9370.89
62_D104_D0.9290.88
120_N124_D0.9220.88
3_L152_D0.9220.88
177_I186_V0.9160.88
74_A81_T0.9150.88
94_Y174_G0.9100.87
122_K125_H0.9090.87
60_L105_M0.9000.87
61_V188_I0.9000.87
115_P118_D0.8920.86
32_I44_I0.8920.86
10_K76_L0.8910.86
11_G137_V0.8910.86
15_D131_I0.8900.86
144_W147_E0.8880.86
125_H182_G0.8770.85
199_T203_G0.8750.85
92_L99_L0.8690.85
14_V174_G0.8630.84
57_L108_L0.8600.84
13_V34_V0.8560.84
98_N198_H0.8550.84
64_D184_R0.8530.84
74_A178_H0.8500.84
29_H34_V0.8500.84
159_N194_S0.8460.83
70_T185_W0.8450.83
51_Q163_F0.8420.83
35_V39_G0.8360.82
178_H181_D0.8350.82
5_N142_E0.8320.82
156_N171_Q0.8290.82
15_D41_D0.8280.82
54_P57_L0.8250.82
136_R178_H0.8180.81
143_R152_D0.8180.81
117_N121_D0.8090.80
19_E31_Q0.8050.80
10_K81_T0.8010.80
31_Q144_W0.8010.80
76_L90_G0.8010.80
88_G91_A0.7990.80
16_R123_L0.7980.79
105_M155_M0.7970.79
35_V38_G0.7900.79
72_A75_A0.7850.78
190_L193_Q0.7820.78
63_D125_H0.7700.77
16_R128_Q0.7690.77
130_A135_A0.7620.76
31_Q45_A0.7600.76
66_R70_T0.7580.76
61_V122_K0.7580.76
128_Q131_I0.7570.76
84_P103_T0.7570.76
11_G36_D0.7570.76
49_K120_N0.7560.76
19_E27_S0.7550.76
99_L187_A0.7500.75
34_V137_V0.7480.75
53_A56_E0.7460.75
19_E23_G0.7420.74
47_N159_N0.7420.74
16_R131_I0.7360.74
176_L190_L0.7350.74
158_G167_N0.7270.73
122_K126_Y0.7240.73
140_F144_W0.7220.72
133_D182_G0.7210.72
120_N123_L0.7170.72
30_Y120_N0.7110.71
174_G188_I0.7080.71
51_Q162_R0.7060.71
19_E22_D0.7040.70
42_Y191_A0.6960.70
28_P50_S0.6960.70
95_I201_D0.6920.69
43_R147_E0.6890.69
162_R165_G0.6880.69
159_N191_A0.6880.69
168_G171_Q0.6870.69
52_D161_G0.6810.68
110_P120_N0.6800.68
67_H98_N0.6790.68
4_K94_Y0.6770.67
29_H33_H0.6770.67
116_D125_H0.6740.67
9_L13_V0.6720.67
78_D133_D0.6720.67
163_F167_N0.6710.67
121_D142_E0.6680.66
103_T160_S0.6670.66
44_I175_L0.6610.66
176_L186_V0.6590.65
83_L96_R0.6560.65
88_G133_D0.6560.65
8_V76_L0.6560.65
114_G117_N0.6540.65
78_D81_T0.6540.65
167_N203_G0.6490.64
9_L42_Y0.6490.64
51_Q56_E0.6480.64
39_G78_D0.6430.63
77_P137_V0.6430.63
67_H198_H0.6430.63
44_I150_I0.6420.63
133_D181_D0.6410.63
2_P168_G0.6340.62
105_M108_L0.6320.62
33_H42_Y0.6310.62
49_K110_P0.6310.62
18_R124_D0.6310.62
33_H144_W0.6280.62
45_A150_I0.6270.61
94_Y190_L0.6260.61
71_A74_A0.6220.61
126_Y177_I0.6210.61
178_H182_G0.6210.61
137_V177_I0.6200.61
84_P87_P0.6180.60
54_P163_F0.6180.60
160_S164_A0.6170.60
33_H43_R0.6160.60
135_A180_P0.6160.60
183_N202_R0.6160.60
14_V36_D0.6130.60
66_R71_A0.6080.59
45_A151_H0.6060.59
9_L153_I0.6050.59
61_V108_L0.6020.58
75_A90_G0.6020.58
129_R184_R0.5990.58
159_N163_F0.5980.58
14_V41_D0.5970.58
46_V127_V0.5950.58
63_D122_K0.5950.58
127_V175_L0.5930.57
108_L122_K0.5920.57
64_D136_R0.5910.57
72_A77_P0.5910.57
177_I188_I0.5880.57
142_E166_D0.5860.56
46_V120_N0.5820.56
113_P118_D0.5820.56
123_L188_I0.5810.56
9_L36_D0.5810.56
152_D171_Q0.5810.56
183_N186_V0.5800.56
128_Q132_A0.5800.56
68_P98_N0.5740.55
83_L171_Q0.5710.54
18_R21_P0.5700.54
175_L190_L0.5670.54
185_W191_A0.5640.54
71_A77_P0.5630.53
127_V153_I0.5610.53
19_E121_D0.5550.52
2_P32_I0.5550.52
125_H133_D0.5550.52
77_P94_Y0.5540.52
55_S108_L0.5540.52
28_P47_N0.5530.52
181_D184_R0.5490.52
57_L190_L0.5490.52
123_L129_R0.5460.51
19_E28_P0.5450.51
1_M6_Y0.5440.51
92_L97_G0.5430.51
12_R136_R0.5410.51
149_G154_H0.5390.50
17_R24_D0.5390.50
38_G78_D0.5370.50
191_A196_S0.5370.50
158_G196_S0.5360.50
84_P202_R0.5360.50
52_D160_S0.5340.50
5_N152_D0.5330.50
195_Q199_T0.5300.49
105_M191_A0.5290.49
93_D141_G0.5260.49
73_L89_G0.5250.49
38_G148_N0.5250.49
138_Y177_I0.5230.48
27_S30_Y0.5230.48
136_R180_P0.5210.48
47_N155_M0.5190.48
196_S199_T0.5180.48
119_L193_Q0.5170.47
92_L177_I0.5160.47
190_L197_W0.5160.47
49_K94_Y0.5150.47
7_G43_R0.5150.47
48_V61_V0.5130.47
2_P39_G0.5130.47
27_S47_N0.5120.47
106_R109_P0.5120.47
66_R183_N0.5120.47
38_G134_E0.5110.47
124_D133_D0.5080.46
22_D132_A0.5080.46
84_P110_P0.5050.46
146_P187_A0.5020.46
11_G38_G0.5010.45
150_I188_I0.5010.45
112_V115_P0.5000.45
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4ccdA 1 0.6305 23.8 0.955 Contact Map
2myyA 1 0.3892 16.6 0.958 Contact Map
2lpdA 1 0.4631 10.7 0.961 Contact Map
2pstX 1 0.2956 7.1 0.964 Contact Map
2khrA 1 0.3596 7.1 0.964 Contact Map
2hj8A 1 0.0936 6.6 0.965 Contact Map
2je2A 2 0.4089 6.2 0.965 Contact Map
3lazA 2 0.1576 5.7 0.966 Contact Map
2yy0A 6 0.2611 5.1 0.967 Contact Map
3e8vA 1 0.2069 4.1 0.968 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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