GREMLIN Database
DUF2277 - Uncharacterized conserved protein (DUF2277)
PFAM: PF10041 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 78 (78)
Sequences: 21007 (6447)
Seq/√Len: 730.0
META: 0.974

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
51_E58_R3.7431.00
54_A58_R2.7551.00
42_E46_D2.4811.00
17_D57_R2.3111.00
57_R61_D2.2401.00
39_K42_E2.1581.00
18_E22_A2.1311.00
21_R50_D2.1031.00
47_R62_S2.0171.00
47_R51_E1.9801.00
31_V56_A1.8921.00
10_N13_P1.8651.00
51_E55_A1.8541.00
71_D74_V1.7091.00
27_F56_A1.7021.00
9_F34_F1.6381.00
51_E54_A1.6261.00
17_D21_R1.5521.00
74_V77_A1.5271.00
20_I57_R1.4441.00
58_R62_S1.4401.00
34_F41_N1.4281.00
43_A47_R1.3961.00
47_R58_R1.3761.00
21_R25_L1.3731.00
38_S41_N1.3571.00
6_K11_F1.2541.00
4_N22_A1.2481.00
28_V52_V1.2451.00
52_V55_A1.1671.00
36_K42_E1.1611.00
46_D50_D1.1551.00
21_R53_A1.1511.00
50_D54_A1.1401.00
32_S59_L1.1001.00
25_L46_D1.0871.00
40_A67_A1.0671.00
32_S45_F1.0461.00
70_R75_E1.0441.00
73_E76_A1.0411.00
45_F49_V1.0231.00
32_S35_T0.9541.00
48_A55_A0.9351.00
44_A62_S0.9311.00
71_D75_E0.9111.00
61_D64_V0.9021.00
54_A57_R0.8771.00
21_R24_A0.8721.00
40_A64_V0.8711.00
25_L49_V0.8601.00
53_A56_A0.8501.00
20_I60_L0.8201.00
7_T33_G0.8021.00
75_E78_K0.8001.00
53_A57_R0.7831.00
55_A58_R0.7811.00
7_T11_F0.7701.00
7_T10_N0.7681.00
44_A48_A0.7661.00
4_N23_A0.7661.00
51_E62_S0.7631.00
34_F38_S0.7461.00
52_V56_A0.7401.00
35_T45_F0.7301.00
54_A61_D0.7181.00
4_N11_F0.7141.00
55_A62_S0.7091.00
15_A19_E0.7061.00
7_T70_R0.7041.00
10_N14_P0.6971.00
73_E77_A0.6961.00
6_K10_N0.6941.00
17_D20_I0.6881.00
23_A31_V0.6761.00
59_L63_L0.6741.00
6_K9_F0.6711.00
18_E21_R0.6691.00
25_L35_T0.6631.00
10_N19_E0.6611.00
11_F16_T0.6521.00
7_T22_A0.6511.00
71_D76_A0.6501.00
41_N64_V0.6471.00
34_F63_L0.6441.00
68_P78_K0.6431.00
9_F65_T0.6421.00
44_A63_L0.6401.00
7_T15_A0.6371.00
56_A60_L0.6331.00
58_R61_D0.5941.00
43_A46_D0.5921.00
24_A52_V0.5921.00
11_F15_A0.5791.00
25_L45_F0.5781.00
57_R64_V0.5771.00
15_A31_V0.5671.00
15_A60_L0.5611.00
68_P71_D0.5571.00
11_F19_E0.5481.00
57_R60_L0.5461.00
4_N25_L0.5431.00
22_A31_V0.5391.00
40_A66_T0.5381.00
9_F60_L0.5321.00
70_R73_E0.5311.00
4_N60_L0.5311.00
37_P42_E0.5301.00
7_T31_V0.5291.00
64_V67_A0.5271.00
66_T71_D0.5261.00
24_A32_S0.5171.00
34_F37_P0.5151.00
10_N67_A0.5131.00
9_F63_L0.5131.00
9_F12_E0.5101.00
66_T70_R0.5101.00
11_F14_P0.5000.99
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2cwdA 1 0.7821 48.7 0.885 Contact Map
1jf8A 1 0.6538 42.8 0.888 Contact Map
1d1qA 1 0.8077 37.7 0.892 Contact Map
1u2pA 1 0.8462 37.2 0.893 Contact Map
1jl3A 1 0.6795 37 0.893 Contact Map
2wjaA 1 0.7821 30.7 0.897 Contact Map
2wmyA 3 0.7949 26.5 0.9 Contact Map
1p8aA 1 0.7692 25.9 0.901 Contact Map
3n8iA 1 0.8077 25 0.901 Contact Map
2fekA 1 0.8333 23 0.903 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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