GREMLIN Database
DUF2272 - Uncharacterized protein conserved in bacteria (DUF2272)
PFAM: PF10030 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 212 (191)
Sequences: 543 (326)
Seq/√Len: 23.6
META: 0.856

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
81_I138_M5.0411.00
156_V168_R4.7581.00
74_S79_D4.2871.00
141_D156_V4.0441.00
141_D168_R3.7831.00
97_D122_D3.5431.00
180_G196_D2.7580.99
96_E211_Q2.6760.99
120_R123_D2.2350.97
172_L200_S2.1830.96
83_A89_A2.1030.95
173_T179_A2.0920.95
87_G91_G2.0320.94
20_L30_A1.9790.94
138_M163_Q1.9750.94
15_L19_L1.9420.93
76_S79_D1.8950.92
173_T177_R1.8940.92
66_A144_V1.8440.91
114_G126_A1.7590.89
96_E101_Y1.7550.89
112_G116_A1.7510.89
62_V142_I1.7340.88
110_C140_C1.6800.86
201_D204_Q1.6720.86
203_R207_F1.6300.85
208_V211_Q1.6240.84
199_C202_N1.6180.84
141_D166_T1.6090.84
146_V149_G1.6070.84
95_A208_V1.6000.84
74_S116_A1.6000.84
150_G157_I1.5790.83
112_G207_F1.5530.82
126_A129_A1.5350.81
81_I136_F1.4660.78
209_L212_L1.4540.77
98_P109_I1.4330.76
16_D116_A1.4330.76
59_I142_I1.4320.76
154_L200_S1.4150.75
9_R13_Y1.4110.75
50_I72_P1.4010.74
30_A157_I1.3560.72
104_R211_Q1.3540.72
31_N39_R1.3390.71
161_V208_V1.3290.70
109_I144_V1.3240.70
64_R73_Y1.3150.69
7_W10_V1.3100.69
17_S51_D1.3060.69
121_F124_L1.2990.69
147_N151_P1.2830.68
96_E104_R1.2790.67
45_C48_A1.2740.67
154_L207_F1.2730.67
25_T28_H1.2710.67
10_V14_W1.2710.67
59_I157_I1.2550.66
59_I108_L1.2330.64
39_R51_D1.2310.64
74_S153_K1.2290.64
60_S203_R1.2020.62
145_A155_Y1.1780.61
17_S76_S1.1760.61
121_F125_P1.1620.60
124_L127_G1.1500.59
112_G163_Q1.1470.59
137_P208_V1.1450.59
88_G91_G1.1430.58
18_G22_S1.1310.58
60_S116_A1.1260.57
202_N205_P1.1250.57
207_F212_L1.1190.57
33_A38_S1.1190.57
83_A164_A1.1170.57
112_G119_L1.1150.56
168_R204_Q1.1130.56
142_I176_G1.0990.55
51_D168_R1.0870.55
197_G201_D1.0840.54
200_S204_Q1.0830.54
8_Q11_G1.0800.54
111_Y168_R1.0800.54
146_V150_G1.0740.54
9_R12_E1.0620.53
85_F121_F1.0350.51
10_V13_Y1.0290.50
27_K35_F1.0270.50
81_I154_L1.0260.50
154_L172_L1.0140.49
17_S60_S1.0140.49
53_A162_D1.0110.49
74_S162_D1.0050.49
201_D209_L0.9900.48
71_F106_G0.9850.47
58_F140_C0.9770.47
29_D35_F0.9770.47
85_F122_D0.9720.46
19_L75_P0.9680.46
131_G134_G0.9610.46
59_I126_A0.9480.45
196_D204_Q0.9480.45
65_Q166_T0.9420.44
97_D173_T0.9390.44
76_S128_L0.9340.44
16_D21_E0.9320.44
111_Y166_T0.9270.43
197_G200_S0.9250.43
150_G174_A0.9240.43
28_H32_G0.9240.43
20_L51_D0.9210.43
61_Y68_A0.9200.43
50_I148_V0.9150.42
38_S118_P0.9140.42
108_L142_I0.9100.42
94_R211_Q0.9060.42
52_Y67_G0.9040.42
200_S207_F0.9000.41
145_A177_R0.8970.41
68_A73_Y0.8910.41
5_G12_E0.8910.41
49_V132_A0.8840.40
86_R91_G0.8840.40
6_L12_E0.8840.40
5_G25_T0.8810.40
16_D19_L0.8800.40
117_A135_G0.8790.40
78_A137_P0.8770.40
31_N166_T0.8750.40
86_R89_A0.8700.39
8_Q41_G0.8690.39
5_G11_G0.8630.39
206_W210_L0.8600.39
112_G138_M0.8580.39
51_D74_S0.8550.38
163_Q203_R0.8530.38
121_F208_V0.8460.38
63_M142_I0.8390.37
112_G203_R0.8350.37
57_A80_Y0.8340.37
111_Y204_Q0.8310.37
138_M207_F0.8280.37
26_G52_Y0.8250.36
89_A94_R0.8250.36
47_A125_P0.8240.36
81_I181_P0.8170.36
41_G114_G0.8110.36
173_T200_S0.8080.35
38_S161_V0.8060.35
9_R14_W0.8040.35
51_D161_V0.8020.35
120_R124_L0.8010.35
47_A180_G0.8000.35
83_A162_D0.7970.35
115_R118_P0.7960.35
155_Y204_Q0.7950.34
36_P39_R0.7920.34
78_A127_G0.7900.34
47_A61_Y0.7870.34
37_A40_D0.7810.34
174_A179_A0.7800.34
24_W164_A0.7770.33
87_G90_S0.7740.33
33_A78_A0.7730.33
207_F211_Q0.7660.33
70_G104_R0.7550.32
58_F165_V0.7500.32
17_S203_R0.7450.31
176_G180_G0.7440.31
16_D22_S0.7440.31
64_R117_A0.7440.31
64_R173_T0.7430.31
12_E16_D0.7400.31
95_A211_Q0.7400.31
174_A200_S0.7390.31
46_R51_D0.7360.31
121_F211_Q0.7170.30
89_A100_A0.7150.30
49_V78_A0.7130.29
93_Y210_L0.7130.29
112_G118_P0.7110.29
81_I178_L0.7110.29
51_D166_T0.7080.29
76_S126_A0.6950.28
19_L22_S0.6860.28
170_L204_Q0.6840.28
26_G40_D0.6750.27
141_D162_D0.6740.27
94_R104_R0.6710.27
119_L168_R0.6690.27
174_A178_L0.6680.27
152_G173_T0.6670.27
73_Y95_A0.6670.27
86_R175_D0.6660.27
97_D177_R0.6660.27
33_A39_R0.6570.26
49_V74_S0.6570.26
31_N51_D0.6540.26
85_F128_L0.6530.26
19_L23_A0.6520.26
199_C205_P0.6510.26
72_P131_G0.6460.26
7_W18_G0.6440.26
86_R196_D0.6440.26
53_A164_A0.6410.25
72_P83_A0.6380.25
5_G8_Q0.6370.25
149_G198_A0.6340.25
85_F145_A0.6310.25
86_R90_S0.6310.25
116_A126_A0.6300.25
113_R158_G0.6270.25
60_S153_K0.6260.25
148_V151_P0.6250.25
49_V61_Y0.6120.24
106_G113_R0.6100.24
15_L34_V0.6090.24
23_A30_A0.6060.24
138_M181_P0.6040.24
58_F110_C0.6020.23
100_A181_P0.6000.23
94_R101_Y0.5990.23
175_D178_L0.5970.23
111_Y178_L0.5960.23
176_G179_A0.5930.23
181_P198_A0.5910.23
8_Q30_A0.5890.23
12_E40_D0.5890.23
106_G140_C0.5860.23
173_T176_G0.5860.23
23_A26_G0.5830.22
142_I171_P0.5820.22
99_E171_P0.5810.22
82_N125_P0.5800.22
6_L11_G0.5790.22
15_L20_L0.5780.22
63_M80_Y0.5770.22
19_L51_D0.5750.22
85_F200_S0.5700.22
97_D179_A0.5680.22
28_H34_V0.5670.22
60_S163_Q0.5650.22
18_G79_D0.5650.22
199_C207_F0.5650.22
29_D33_A0.5640.22
74_S149_G0.5640.22
90_S175_D0.5630.22
62_V117_A0.5620.21
61_Y157_I0.5610.21
24_W27_K0.5590.21
163_Q204_Q0.5580.21
6_L17_S0.5580.21
148_V166_T0.5570.21
57_A83_A0.5520.21
63_M124_L0.5510.21
79_D128_L0.5490.21
156_V178_L0.5480.21
92_A211_Q0.5450.21
50_I81_I0.5450.21
38_S94_R0.5450.21
15_L79_D0.5420.21
23_A29_D0.5420.21
70_G123_D0.5400.20
176_G197_G0.5400.20
20_L127_G0.5390.20
163_Q166_T0.5350.20
56_A106_G0.5350.20
155_Y169_H0.5330.20
132_A197_G0.5320.20
22_S37_A0.5310.20
98_P111_Y0.5300.20
145_A149_G0.5290.20
33_A114_G0.5280.20
204_Q212_L0.5280.20
49_V166_T0.5270.20
112_G136_F0.5250.20
31_N37_A0.5220.20
145_A167_M0.5100.19
92_A98_P0.5100.19
72_P197_G0.5090.19
90_S93_Y0.5070.19
46_R84_A0.5030.19
59_I141_D0.5030.19
123_D205_P0.5030.19
85_F154_L0.5000.19
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3vpiA 1 0.6179 78.7 0.941 Contact Map
2if6A 1 0.4434 42.6 0.954 Contact Map
4cgkA 3 0.6462 39.1 0.955 Contact Map
4ct3A 4 0.7075 23.5 0.959 Contact Map
4hz9A 1 0.5283 20.7 0.96 Contact Map
4hpeA 1 0.6509 18.5 0.961 Contact Map
4lj1A 1 0.5991 18.4 0.961 Contact Map
4q4gX 1 0.6038 15.2 0.963 Contact Map
4fdyA 1 0.6415 12.4 0.964 Contact Map
2k3aA 1 0.6226 8.5 0.967 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.007 seconds.