GREMLIN Database
DUF2270 - Predicted integral membrane protein (DUF2270)
PFAM: PF10028 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 184 (183)
Sequences: 2060 (1202)
Seq/√Len: 88.8
META: 0.872

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
55_T131_F4.8231.00
39_S42_A3.0981.00
105_D112_K2.8431.00
59_L115_F2.7981.00
16_T20_T2.7431.00
141_K145_H2.6961.00
146_P158_R2.5841.00
177_Y180_L2.5011.00
13_V16_T2.4491.00
159_A166_G2.3951.00
2_H5_R2.2231.00
173_G177_Y2.1661.00
73_A112_K2.1521.00
156_V170_L2.1101.00
151_S154_E2.0801.00
85_P89_P2.0721.00
69_D112_K2.0681.00
154_E157_E2.0541.00
149_A155_F1.8871.00
7_E10_R1.8731.00
150_T154_E1.8431.00
49_L57_F1.8101.00
142_I159_A1.7881.00
29_T54_V1.7581.00
51_M134_L1.7531.00
65_Y121_R1.7321.00
44_H138_W1.7291.00
165_P168_L1.7281.00
32_A50_V1.7231.00
9_Y13_V1.7121.00
174_V178_A1.6461.00
70_V73_A1.6391.00
56_L133_I1.6251.00
77_L81_N1.6211.00
157_E160_A1.6091.00
179_A182_A1.5971.00
58_L119_L1.5701.00
114_S117_E1.5651.00
161_I169_V1.5281.00
69_D117_E1.5171.00
10_R15_R1.4580.99
51_M173_G1.4580.99
177_Y181_I1.4510.99
104_E108_R1.4260.99
69_D72_R1.4210.99
55_T180_L1.4080.99
59_L119_L1.4030.99
43_P141_K1.3930.99
87_L99_A1.3880.99
100_E104_E1.3850.99
88_R107_R1.3740.99
146_P149_A1.3670.99
95_D105_D1.3540.99
54_V134_L1.3520.99
3_L83_F1.3440.99
178_A182_A1.3390.99
48_L173_G1.3350.99
171_A178_A1.3180.99
156_V166_G1.3140.99
173_G176_F1.3100.99
3_L106_L1.3060.99
135_L173_G1.3050.99
174_V177_Y1.2960.99
14_W71_W1.2930.99
3_L6_G1.2150.98
65_Y133_I1.2130.98
66_R109_P1.2080.98
152_L170_L1.2060.98
9_Y12_T1.2060.98
37_F45_L1.2040.98
67_Y111_F1.1960.98
135_L177_Y1.1920.98
139_L170_L1.1800.98
153_A157_E1.1790.98
156_V167_W1.1770.98
79_E121_R1.1750.97
72_R121_R1.1590.97
10_R14_W1.1520.97
168_L172_A1.1370.97
97_D100_E1.1270.97
156_V159_A1.1080.96
53_L71_W1.0950.96
150_T153_A1.0660.96
40_P131_F1.0590.95
168_L178_A1.0580.95
69_D77_L1.0580.95
25_A29_T1.0440.95
86_M90_E1.0430.95
79_E106_L1.0330.95
35_F42_A1.0240.94
123_L128_L1.0180.94
1_A106_L1.0170.94
147_T165_P1.0070.94
142_I155_F1.0060.94
167_W171_A1.0030.94
73_A110_R1.0010.94
133_I159_A1.0000.94
53_L129_W0.9980.94
97_D101_L0.9970.94
10_R60_I0.9920.93
46_V138_W0.9510.92
67_Y121_R0.9500.92
149_A153_A0.9430.92
50_V130_I0.9270.91
152_L156_V0.9260.91
34_S38_S0.9230.91
155_F170_L0.9210.91
45_L134_L0.9180.90
4_Y12_T0.9150.90
128_L177_Y0.9090.90
30_G53_L0.8970.89
60_I81_N0.8960.89
31_A35_F0.8920.89
105_D110_R0.8920.89
78_L83_F0.8900.89
22_T28_T0.8880.89
52_L136_L0.8850.89
11_S71_W0.8830.89
90_E124_R0.8790.88
142_I177_Y0.8720.88
30_G50_V0.8690.88
159_A170_L0.8670.88
179_A183_L0.8660.88
80_T112_K0.8650.88
135_L181_I0.8590.87
89_P107_R0.8550.87
8_V75_V0.8530.87
168_L171_A0.8520.87
26_V57_F0.8450.86
169_V172_A0.8370.86
51_M135_L0.8360.86
123_L177_Y0.8230.85
84_A103_A0.8190.85
56_L60_I0.8110.84
129_W132_L0.8090.84
12_T75_V0.8060.84
136_L139_L0.8040.84
90_E99_A0.8020.83
123_L181_I0.8010.83
70_V105_D0.7950.83
85_P107_R0.7940.83
43_P131_F0.7890.82
81_N98_W0.7800.82
65_Y113_I0.7750.81
119_L183_L0.7640.80
116_W183_L0.7610.80
78_L86_M0.7580.80
103_A146_P0.7570.80
116_W143_T0.7570.80
76_R109_P0.7550.80
113_I121_R0.7490.79
154_E158_R0.7430.78
13_V20_T0.7350.78
26_V37_F0.7330.78
58_L133_I0.7330.78
55_T119_L0.7330.78
160_A171_A0.7300.77
51_M131_F0.7280.77
172_A175_L0.7170.76
157_E170_L0.7130.76
160_A167_W0.7110.75
83_F117_E0.7100.75
167_W177_Y0.7100.75
150_T160_A0.7070.75
7_E12_T0.7060.75
40_P141_K0.7060.75
55_T59_L0.7030.75
133_I137_A0.6960.74
31_A138_W0.6950.74
29_T123_L0.6940.74
18_L21_T0.6930.74
88_R103_A0.6890.73
29_T106_L0.6870.73
34_S43_P0.6830.72
55_T179_A0.6810.72
90_E94_P0.6790.72
132_L164_I0.6790.72
70_V76_R0.6770.72
33_L37_F0.6760.72
35_F68_Y0.6740.71
132_L171_A0.6720.71
117_E181_I0.6690.71
135_L139_L0.6670.71
93_H96_P0.6650.70
90_E102_L0.6580.70
3_L43_P0.6570.69
3_L28_T0.6550.69
32_A79_E0.6550.69
76_R114_S0.6540.69
152_L164_I0.6530.69
4_Y13_V0.6490.69
171_A175_L0.6490.69
18_L41_E0.6470.68
25_A176_F0.6440.68
11_S16_T0.6440.68
55_T176_F0.6410.68
29_T35_F0.6360.67
143_T155_F0.6340.67
120_G158_R0.6310.66
6_G112_K0.6280.66
66_R137_A0.6280.66
38_S141_K0.6270.66
20_T32_A0.6250.66
85_P105_D0.6230.65
13_V26_V0.6210.65
42_A46_V0.6210.65
170_L178_A0.6200.65
15_R61_E0.6190.65
102_L123_L0.6160.65
39_S130_I0.6130.64
109_P137_A0.6130.64
12_T71_W0.6090.64
36_A47_L0.6090.64
175_L179_A0.6050.63
81_N94_P0.6050.63
27_V31_A0.6030.63
4_Y93_H0.5970.62
69_D73_A0.5940.62
51_M177_Y0.5940.62
102_L129_W0.5940.62
172_A178_A0.5900.61
143_T159_A0.5900.61
84_A89_P0.5900.61
89_P124_R0.5880.61
119_L180_L0.5880.61
58_L62_A0.5870.61
113_I117_E0.5860.61
147_T155_F0.5850.61
45_L142_I0.5850.61
44_H141_K0.5830.60
69_D105_D0.5790.60
2_H6_G0.5790.60
149_A167_W0.5780.60
71_W121_R0.5750.59
145_H158_R0.5720.59
7_E74_R0.5720.59
36_A46_V0.5650.58
73_A76_R0.5640.58
29_T37_F0.5640.58
163_P170_L0.5640.58
35_F38_S0.5600.57
123_L180_L0.5600.57
46_V158_R0.5590.57
106_L138_W0.5580.57
73_A77_L0.5550.57
117_E120_G0.5500.56
29_T77_L0.5480.56
159_A173_G0.5480.56
103_A107_R0.5460.55
32_A67_Y0.5450.55
166_G169_V0.5450.55
33_L137_A0.5430.55
21_T74_R0.5390.54
68_Y118_A0.5380.54
76_R137_A0.5380.54
94_P99_A0.5330.54
159_A167_W0.5290.53
55_T128_L0.5270.53
81_N101_L0.5260.53
81_N105_D0.5260.53
131_F176_F0.5230.52
82_F86_M0.5220.52
68_Y83_F0.5200.52
153_A182_A0.5190.52
94_P119_L0.5150.51
139_L143_T0.5130.51
50_V134_L0.5110.51
3_L7_E0.5090.50
136_L157_E0.5080.50
78_L89_P0.5080.50
99_A112_K0.5030.50
1_A131_F0.5020.49
43_P51_M0.5000.49
59_L105_D0.5000.49
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1zoyC 1 0.4728 18.7 0.959 Contact Map
4k0jA 3 0.3696 16.6 0.96 Contact Map
2h88C 2 0.4783 15.4 0.961 Contact Map
2l8sA 1 0.1467 7.1 0.966 Contact Map
4b03D 5 0.3207 3.5 0.971 Contact Map
2wdqC 1 0.3967 3.2 0.971 Contact Map
4ysxC 1 0.462 3.2 0.972 Contact Map
2k1aA 1 0.1522 2.8 0.972 Contact Map
2m20A 2 0.1196 2.8 0.972 Contact Map
4ytpC 1 0.4783 2.4 0.973 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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