GREMLIN Database
DUF2269 - Predicted integral membrane protein (DUF2269)
PFAM: PF10027 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 150 (149)
Sequences: 12952 (9697)
Seq/√Len: 794.4
META: 0.917

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
64_L68_H3.3871.00
144_W148_A3.1781.00
77_G81_A2.6021.00
3_L65_W2.4741.00
10_G58_L2.3541.00
99_L103_L2.3311.00
65_W69_L2.2701.00
5_F144_W2.1881.00
109_A113_A2.0921.00
80_L84_V2.0461.00
36_A39_A2.0411.00
46_R50_L1.8931.00
6_L62_T1.8301.00
12_V143_V1.7711.00
91_A95_P1.7711.00
108_E112_A1.7581.00
120_E124_L1.7531.00
100_Q104_R1.7421.00
67_A79_I1.6901.00
101_I105_R1.6771.00
73_P76_T1.6631.00
82_S145_L1.6491.00
25_M29_R1.6471.00
100_Q103_L1.6341.00
82_S146_M1.6151.00
76_T80_L1.5991.00
99_L102_R1.5961.00
90_G94_L1.5841.00
45_V49_W1.5781.00
21_I24_F1.5211.00
141_A145_L1.5061.00
60_L86_Y1.5051.00
94_L98_W1.4931.00
58_L62_T1.4661.00
129_F133_W1.4521.00
122_R125_A1.4441.00
126_R130_A1.3881.00
75_G80_L1.3671.00
92_L131_L1.3631.00
85_L138_L1.3541.00
102_R106_L1.3521.00
79_I83_L1.3511.00
31_S36_A1.3451.00
102_R105_R1.3301.00
77_G80_L1.3251.00
8_V11_A1.3161.00
101_I104_R1.3131.00
104_R108_E1.3061.00
33_D36_A1.3051.00
29_R121_Y1.2931.00
10_G62_T1.2901.00
133_W137_L1.2791.00
27_R43_L1.2551.00
123_R127_R1.2411.00
130_A133_W1.2381.00
105_R108_E1.2301.00
61_V65_W1.2261.00
103_L107_A1.1821.00
5_F9_L1.1771.00
39_A43_L1.1681.00
101_I106_L1.1681.00
99_L127_R1.1641.00
17_G20_G1.1621.00
34_P38_A1.1401.00
89_A138_L1.1391.00
99_L124_L1.1321.00
119_P123_R1.1321.00
109_A112_A1.1231.00
105_R109_A1.1131.00
118_P121_Y1.1111.00
82_S142_I1.1041.00
4_K66_L1.1011.00
127_R131_L1.0911.00
72_W76_T1.0851.00
95_P98_W1.0711.00
55_A58_L1.0671.00
65_W68_H1.0611.00
11_A59_Q1.0561.00
127_R130_A1.0461.00
8_V147_V1.0371.00
13_V55_A1.0331.00
117_L120_E1.0301.00
84_V88_V1.0201.00
5_F148_A1.0161.00
60_L64_L1.0121.00
106_L110_A1.0051.00
39_A42_R1.0051.00
121_Y125_A1.0031.00
34_P37_I0.9991.00
62_T66_L0.9951.00
120_E123_R0.9941.00
64_L83_L0.9891.00
102_R120_E0.9881.00
59_Q62_T0.9791.00
130_A134_P0.9781.00
93_G98_W0.9751.00
125_A129_F0.9671.00
123_R126_R0.9541.00
67_A74_L0.9331.00
87_V91_A0.9331.00
104_R107_A0.9291.00
6_L10_G0.9211.00
81_A145_L0.9131.00
111_A114_G0.9071.00
119_P122_R0.8941.00
103_L125_A0.8921.00
85_L142_I0.8901.00
68_H74_L0.8841.00
43_L46_R0.8801.00
62_T65_W0.8551.00
27_R31_S0.8551.00
35_A39_A0.8451.00
12_V15_F0.8441.00
99_L104_R0.8401.00
118_P122_R0.8141.00
80_L83_L0.8141.00
50_L54_P0.8121.00
60_L83_L0.8091.00
134_P138_L0.7921.00
67_A70_A0.7921.00
98_W102_R0.7851.00
134_P137_L0.7811.00
11_A143_V0.7791.00
74_L83_L0.7761.00
29_R125_A0.7741.00
26_L30_R0.7711.00
102_R124_L0.7671.00
10_G55_A0.7671.00
91_A94_L0.7621.00
101_I108_E0.7531.00
42_R108_E0.7371.00
132_G135_A0.7361.00
78_W82_S0.7331.00
5_F140_L0.7311.00
24_F43_L0.7281.00
51_F54_P0.7201.00
27_R30_R0.7191.00
145_L150_P0.7121.00
131_L135_A0.7081.00
93_G97_V0.7061.00
107_A110_A0.7021.00
106_L109_A0.7011.00
13_V17_G0.7001.00
120_E126_R0.6991.00
128_W132_G0.6981.00
83_L87_V0.6951.00
99_L106_L0.6941.00
18_G22_A0.6921.00
122_R126_R0.6921.00
89_A135_A0.6901.00
103_L108_E0.6901.00
9_L140_L0.6871.00
86_Y89_A0.6861.00
27_R46_R0.6861.00
3_L69_L0.6771.00
16_G19_L0.6761.00
52_T55_A0.6701.00
40_V43_L0.6661.00
92_L95_P0.6641.00
22_A136_F0.6611.00
98_W101_I0.6611.00
103_L106_L0.6611.00
32_G122_R0.6571.00
128_W131_L0.6551.00
72_W149_K0.6551.00
28_A40_V0.6541.00
42_R46_R0.6541.00
53_A56_V0.6521.00
19_L22_A0.6511.00
93_G96_V0.6491.00
13_V16_G0.6471.00
4_K8_V0.6401.00
120_E125_A0.6351.00
133_W136_F0.6321.00
66_L70_A0.6321.00
108_E111_A0.6291.00
38_A42_R0.6281.00
56_V90_G0.6281.00
81_A141_A0.6261.00
18_G21_I0.6231.00
142_I145_L0.6231.00
26_L29_R0.6191.00
57_L61_V0.6151.00
141_A144_W0.6131.00
14_L18_G0.6121.00
53_A57_L0.6081.00
48_D53_A0.6061.00
14_L17_G0.5971.00
117_L122_R0.5951.00
28_A31_S0.5931.00
64_L74_L0.5911.00
52_T56_V0.5861.00
20_G24_F0.5851.00
20_G47_A0.5821.00
56_V139_L0.5801.00
68_H71_G0.5791.00
45_V48_D0.5771.00
59_Q66_L0.5761.00
13_V19_L0.5721.00
6_L58_L0.5671.00
24_F40_V0.5661.00
85_L145_L0.5651.00
64_L67_A0.5631.00
44_V48_D0.5571.00
36_A40_V0.5551.00
106_L120_E0.5511.00
135_A139_L0.5481.00
110_A118_P0.5471.00
15_F19_L0.5451.00
110_A113_A0.5381.00
10_G59_Q0.5361.00
100_Q107_A0.5291.00
128_W135_A0.5231.00
2_L148_A0.5181.00
117_L121_Y0.5131.00
81_A85_L0.5091.00
137_L141_A0.5071.00
34_P41_A0.5061.00
89_A139_L0.5041.00
104_R109_A0.5041.00
17_G52_T0.5031.00
55_A59_Q0.5031.00
119_P124_L0.5021.00
35_A38_A0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2xq2A 2 0.6467 27.4 0.88 Contact Map
3mk7C 2 0.5067 14.4 0.894 Contact Map
4gd3A 2 0.6267 9.7 0.902 Contact Map
4c7rA 3 0.1733 7.3 0.908 Contact Map
3dh4A 3 0.6733 7.2 0.908 Contact Map
2rddB 1 0.1533 5.9 0.912 Contact Map
3o7qA 1 0.9467 5.7 0.912 Contact Map
4mt1A 3 0.7133 4.8 0.915 Contact Map
4w6vA 1 0.66 4.7 0.915 Contact Map
4hkrA 3 0.6133 4.3 0.917 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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