GREMLIN Database
DUF2259 - Predicted secreted protein (DUF2259)
PFAM: PF10016 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 188 (187)
Sequences: 1238 (964)
Seq/√Len: 70.5
META: 0.867

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
4_Q17_E7.1891.00
165_I186_T5.1751.00
138_K141_P3.9121.00
172_R179_D3.6021.00
8_Q13_F3.1231.00
174_G179_D3.0641.00
8_Q11_S2.8611.00
167_V184_V2.6381.00
109_E143_S2.5001.00
153_K182_Y2.3461.00
119_R136_E2.3421.00
3_E16_A2.2551.00
20_V29_V2.2311.00
34_I54_A2.1661.00
4_Q19_Y2.1651.00
141_P144_R2.0511.00
34_I57_L2.0431.00
169_E180_I1.9061.00
7_V14_P1.8941.00
23_V186_T1.8181.00
139_G142_Q1.8151.00
23_V163_S1.8031.00
65_F71_P1.7681.00
174_G177_G1.7341.00
93_D149_S1.6961.00
119_R133_T1.6921.00
17_E35_R1.6560.99
49_R53_K1.6210.99
164_L186_T1.6190.99
58_L63_I1.6190.99
129_G132_Y1.6180.99
118_F122_L1.5980.99
166_F187_L1.5740.99
137_D140_L1.5540.99
170_M183_M1.4950.99
152_I168_I1.4950.99
94_F136_E1.4920.99
159_P163_S1.4350.99
16_A47_R1.4340.99
110_G116_R1.4330.99
14_P41_Q1.4280.98
21_V184_V1.4240.98
19_Y28_F1.4080.98
41_Q46_A1.3920.98
126_D134_V1.3690.98
3_E47_R1.3650.98
134_V137_D1.3590.98
12_G15_Y1.3540.98
108_I111_S1.3520.98
118_F127_G1.3510.98
16_A43_G1.3370.98
34_I50_L1.3120.97
171_K180_I1.2990.97
75_S78_G1.2960.97
133_T136_E1.2850.97
6_G15_Y1.2830.97
102_C146_C1.2650.97
26_N32_G1.2590.97
9_D12_G1.2570.97
18_I54_A1.2480.96
124_S129_G1.2370.96
157_I164_L1.2320.96
158_D162_S1.2300.96
144_R150_Y1.2080.96
166_F186_T1.2040.96
72_L154_R1.1950.95
47_R51_L1.1820.95
16_A50_L1.1800.95
3_E50_L1.1790.95
138_K142_Q1.1730.95
23_V26_N1.1690.95
115_V148_L1.1670.95
111_S176_E1.1540.94
14_P46_A1.1390.94
95_E116_R1.1290.94
169_E182_Y1.1280.94
22_D25_T1.1220.94
10_G176_E1.1210.94
36_Y50_L1.0970.93
137_D144_R1.0480.91
44_S48_Y1.0350.90
120_L134_V1.0180.90
15_Y181_R1.0080.89
129_G134_V0.9890.88
2_F5_Y0.9820.88
183_M186_T0.9810.88
109_E112_G0.9800.88
26_N33_P0.9660.87
91_F94_F0.9640.87
34_I53_K0.9630.87
75_S79_D0.9540.87
10_G175_F0.9490.86
9_D13_F0.9450.86
120_L125_R0.9430.86
76_L81_P0.9410.86
177_G181_R0.9410.86
9_D175_F0.9400.86
118_F185_E0.9200.84
89_I94_F0.9190.84
20_V28_F0.9160.84
180_I183_M0.9150.84
181_R184_V0.9120.84
36_Y53_K0.9120.84
20_V183_M0.9110.84
54_A58_L0.9110.84
14_P157_I0.9080.84
135_H186_T0.9050.83
7_V12_G0.9030.83
117_S168_I0.8890.82
158_D161_G0.8810.82
174_G178_P0.8780.82
8_Q14_P0.8770.82
99_S103_E0.8760.81
170_M182_Y0.8720.81
3_E8_Q0.8690.81
18_I50_L0.8660.81
127_G131_T0.8620.80
149_S154_R0.8620.80
128_G131_T0.8590.80
49_R52_A0.8580.80
55_A59_E0.8530.80
6_G181_R0.8510.80
154_R157_I0.8460.79
11_S176_E0.8440.79
58_L61_Y0.8430.79
12_G175_F0.8400.79
156_L165_I0.8330.78
126_D130_K0.8270.78
118_F135_H0.8230.77
61_Y179_D0.8210.77
72_L75_S0.8180.77
8_Q12_G0.8170.77
108_I143_S0.8170.77
156_L167_V0.8120.76
26_N117_S0.8100.76
48_Y52_A0.8070.76
145_G173_Q0.8030.75
161_G164_L0.8000.75
133_T137_D0.7960.75
26_N143_S0.7960.75
46_A59_E0.7900.74
148_L178_P0.7880.74
71_P74_I0.7860.74
13_F33_P0.7810.73
160_G163_S0.7790.73
109_E113_Q0.7790.73
64_E69_G0.7760.73
28_F147_P0.7690.72
5_Y31_G0.7660.72
3_E25_T0.7650.72
127_G130_K0.7640.72
73_Y79_D0.7640.72
23_V187_L0.7630.72
67_N79_D0.7590.71
41_Q45_G0.7520.71
2_F6_G0.7350.69
10_G13_F0.7350.69
15_Y35_R0.7340.69
81_P151_R0.7330.69
11_S174_G0.7320.68
33_P36_Y0.7290.68
145_G172_R0.7280.68
110_G114_N0.7250.68
108_I112_G0.7220.67
20_V60_R0.7220.67
46_A66_L0.7210.67
64_E67_N0.7190.67
49_R131_T0.7160.67
158_D169_E0.7160.67
66_L69_G0.7120.66
125_R131_T0.7070.66
33_P54_A0.7030.65
4_Q181_R0.7020.65
2_F21_V0.6900.64
25_T38_D0.6890.64
147_P172_R0.6870.64
45_G48_Y0.6860.63
111_S114_N0.6800.63
28_F33_P0.6790.63
121_E131_T0.6780.63
146_C175_F0.6740.62
40_G44_S0.6720.62
118_F121_E0.6710.62
51_L63_I0.6700.62
43_G46_A0.6650.61
74_I77_D0.6630.61
96_S117_S0.6610.61
102_C166_F0.6590.60
79_D82_P0.6590.60
82_P98_A0.6560.60
108_I173_Q0.6480.59
42_D169_E0.6470.59
6_G141_P0.6460.59
16_A100_Y0.6460.59
108_I176_E0.6450.59
85_G89_I0.6420.58
71_P88_T0.6390.58
143_S146_C0.6380.58
22_D92_R0.6340.57
43_G47_R0.6340.57
96_S115_V0.6300.57
6_G19_Y0.6260.56
38_D41_Q0.6250.56
28_F32_G0.6250.56
118_F124_S0.6230.56
15_Y40_G0.6220.56
53_K113_Q0.6200.56
74_I89_I0.6200.56
137_D141_P0.6180.56
92_R119_R0.6170.55
111_S123_T0.6160.55
53_K122_L0.6150.55
42_D50_L0.6150.55
129_G162_S0.6150.55
120_L127_G0.6130.55
141_P146_C0.6130.55
124_S188_R0.6040.54
11_S146_C0.6030.54
28_F150_Y0.5940.53
45_G99_S0.5930.52
5_Y47_R0.5850.52
22_D26_N0.5820.51
140_L147_P0.5820.51
123_T134_V0.5780.51
87_E90_E0.5780.51
122_L164_L0.5750.50
93_D167_V0.5740.50
75_S81_P0.5730.50
84_D89_I0.5730.50
68_Q74_I0.5720.50
115_V173_Q0.5670.49
44_S57_L0.5660.49
155_V166_F0.5660.49
79_D168_I0.5650.49
5_Y183_M0.5650.49
29_V32_G0.5650.49
138_K159_P0.5630.49
113_Q116_R0.5610.49
40_G46_A0.5590.48
36_Y46_A0.5590.48
69_G73_Y0.5570.48
71_P179_D0.5520.48
117_S147_P0.5490.47
19_Y32_G0.5490.47
77_D80_P0.5460.47
102_C106_P0.5450.47
89_I102_C0.5450.47
26_N135_H0.5440.47
57_L61_Y0.5420.46
109_E116_R0.5410.46
63_I115_V0.5400.46
44_S58_L0.5400.46
123_T130_K0.5390.46
36_Y39_E0.5380.46
26_N36_Y0.5370.46
100_Y147_P0.5350.45
42_D152_I0.5340.45
127_G132_Y0.5310.45
110_G177_G0.5290.45
135_H139_G0.5270.44
163_S188_R0.5250.44
35_R156_L0.5240.44
128_G134_V0.5220.44
69_G93_D0.5190.44
146_C177_G0.5190.44
18_I47_R0.5180.43
14_P179_D0.5140.43
48_Y51_L0.5130.43
63_I90_E0.5130.43
50_L154_R0.5130.43
10_G120_L0.5130.43
164_L187_L0.5110.43
115_V146_C0.5090.42
10_G131_T0.5060.42
11_S175_F0.5050.42
8_Q35_R0.5030.42
152_I164_L0.5020.42
56_P60_R0.5000.41
122_L134_V0.5000.41
22_D28_F0.5000.41
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3odgA 3 0.3245 7.2 0.951 Contact Map
2b94A 4 0.2713 5.1 0.954 Contact Map
1zd0A 1 0.2553 4.6 0.955 Contact Map
4gqcA 4 0.3032 4.4 0.956 Contact Map
4nawD 1 0.0372 4.1 0.956 Contact Map
3fucA 3 0.3351 3.9 0.957 Contact Map
4uc0A 3 0.2819 3.7 0.957 Contact Map
3fq3A 6 0.4894 3.6 0.958 Contact Map
3brcA 4 0.2926 3.2 0.959 Contact Map
1vmkA 3 0.2979 3 0.959 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0072 seconds.