GREMLIN Database
DUF2251 - Uncharacterized protein conserved in bacteria (DUF2251)
PFAM: PF10008 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 93 (90)
Sequences: 1088 (837)
Seq/√Len: 88.2
META: 0.947

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
67_A74_P4.6511.00
11_V66_A3.7721.00
78_F85_G3.4791.00
15_D41_Y2.4391.00
3_D10_A2.4341.00
20_G41_Y2.2111.00
26_D29_R2.1761.00
39_H42_N2.1081.00
4_S63_G2.0981.00
58_V67_A2.0431.00
22_F68_L1.8041.00
87_C90_G1.7891.00
64_L81_A1.7861.00
22_F57_I1.7001.00
24_A85_G1.6661.00
10_A27_R1.6421.00
22_F78_F1.4660.99
23_Y34_I1.4560.99
5_P63_G1.4500.99
29_R35_L1.4000.99
79_D82_A1.3880.99
14_E19_T1.3540.99
24_A80_F1.2950.99
25_L33_P1.2790.99
62_D65_K1.2580.98
58_V69_L1.2240.98
7_G35_L1.2190.98
57_I68_L1.1800.97
36_D80_F1.1660.97
15_D80_F1.1610.97
38_L87_C1.1510.97
8_P28_S1.1420.97
67_A72_G1.1300.97
12_V37_A1.1130.96
46_V71_N1.0850.96
41_Y80_F1.0640.95
69_L72_G1.0530.95
14_E23_Y1.0410.95
51_R54_D1.0410.95
40_I76_A1.0370.95
47_T53_S1.0180.94
40_I68_L1.0090.94
21_Y91_F1.0060.94
19_T37_A1.0040.94
46_V73_Y0.9970.93
56_E69_L0.9900.93
84_R90_G0.9810.93
15_D20_G0.9750.93
60_S65_K0.9480.92
46_V75_H0.9470.92
7_G10_A0.9400.91
28_S55_V0.9280.91
48_D70_I0.9270.91
4_S7_G0.9250.91
42_N89_T0.9240.91
60_S64_L0.9210.90
73_Y88_R0.9160.90
47_T50_D0.9010.89
47_T51_R0.8970.89
19_T42_N0.8940.89
44_A51_R0.8910.89
39_H89_T0.8810.88
8_P72_G0.8690.88
11_V25_L0.8690.88
42_N45_D0.8670.88
22_F60_S0.8360.86
84_R89_T0.8350.86
53_S71_N0.8260.85
12_V32_Q0.8190.84
9_Y64_L0.8140.84
14_E37_A0.7950.83
71_N75_H0.7850.82
65_K79_D0.7800.81
81_A89_T0.7750.81
52_P56_E0.7670.80
78_F83_R0.7300.77
36_D71_N0.7140.75
31_G37_A0.7080.75
68_L76_A0.7080.75
38_L58_V0.7070.75
24_A78_F0.7070.75
6_E63_G0.7020.74
12_V66_A0.7000.74
22_F76_A0.6980.74
25_L32_Q0.6920.73
28_S82_A0.6910.73
49_R71_N0.6810.72
11_V74_P0.6810.72
77_V86_Y0.6810.72
26_D80_F0.6780.72
37_A76_A0.6780.72
58_V72_G0.6690.71
9_Y25_L0.6640.70
44_A81_A0.6580.69
30_E57_I0.6540.69
56_E72_G0.6510.69
69_L74_P0.6480.68
23_Y37_A0.6470.68
5_P45_D0.6390.67
10_A35_L0.6280.66
52_P57_I0.6230.65
66_A83_R0.6140.64
79_D84_R0.6120.64
18_E31_G0.6120.64
4_S20_G0.6070.63
11_V78_F0.6040.63
43_V52_P0.6040.63
67_A84_R0.5930.61
11_V24_A0.5920.61
12_V33_P0.5900.61
10_A33_P0.5860.60
19_T23_Y0.5840.60
27_R35_L0.5750.59
38_L90_G0.5690.58
53_S70_I0.5670.58
18_E50_D0.5620.57
66_A76_A0.5620.57
40_I70_I0.5570.57
67_A90_G0.5530.56
51_R73_Y0.5530.56
37_A91_F0.5500.56
20_G81_A0.5440.55
4_S90_G0.5390.54
16_D19_T0.5360.54
82_A86_Y0.5350.54
60_S75_H0.5350.54
65_K77_V0.5320.53
38_L76_A0.5300.53
83_R90_G0.5290.53
30_E50_D0.5150.51
36_D92_P0.5100.50
7_G24_A0.5030.49
43_V46_V0.5020.49
62_D71_N0.5000.49
61_E72_G0.5000.49
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3pe7A 1 0.871 64.4 0.879 Contact Map
2bklA 1 0.9355 58.3 0.884 Contact Map
2madH 2 0.8602 56 0.885 Contact Map
4a5sA 2 0.8602 51 0.889 Contact Map
2hqsA 2 0.871 47.5 0.891 Contact Map
3c5mA 1 0.871 45.1 0.893 Contact Map
1yr2A 1 0.8817 43.8 0.893 Contact Map
2ecfA 2 0.9032 39.9 0.896 Contact Map
1jmxB 2 0.8172 39.6 0.896 Contact Map
3iujA 1 0.9247 39.6 0.896 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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