GREMLIN Database
DUF2244 - Integral membrane protein (DUF2244)
PFAM: PF10003 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 138 (138)
Sequences: 8292 (5474)
Seq/√Len: 466.0
META: 0.911

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
72_E87_E3.7451.00
63_R78_V2.4871.00
55_L59_H2.3811.00
74_R85_E2.2741.00
110_R115_R2.2491.00
51_W55_L2.1631.00
76_E85_E2.0661.00
96_E110_R1.9991.00
74_R87_E1.9811.00
64_E77_R1.9341.00
76_E83_V1.8051.00
31_L39_L1.7931.00
78_V83_V1.7511.00
13_L17_A1.7441.00
19_L49_L1.7441.00
65_R76_E1.6821.00
29_A33_L1.6641.00
73_L90_P1.6631.00
129_S133_A1.6611.00
23_S42_A1.6501.00
67_T74_R1.6451.00
37_P40_P1.6271.00
108_T115_R1.5751.00
20_A46_V1.5591.00
50_Y54_R1.5521.00
106_R117_E1.5401.00
79_S82_R1.4981.00
131_A135_R1.4801.00
68_L73_L1.4741.00
88_F93_V1.4661.00
130_L134_L1.4651.00
8_S11_G1.4591.00
77_R84_R1.4481.00
75_V116_V1.4161.00
1_L121_F1.4081.00
94_R110_R1.4081.00
16_M50_Y1.3881.00
12_F16_M1.3851.00
10_R14_L1.3801.00
96_E115_R1.3801.00
123_S126_E1.3661.00
93_V109_L1.3651.00
107_L131_A1.3641.00
86_W116_V1.2991.00
14_L18_A1.2891.00
28_L32_L1.2861.00
12_F57_Y1.2771.00
48_L52_A1.2591.00
47_L51_W1.2541.00
70_P90_P1.2481.00
7_L11_G1.2421.00
100_P105_I1.2281.00
44_L48_L1.2221.00
108_T117_E1.2171.00
42_A45_E1.2111.00
14_L17_A1.2041.00
77_R86_W1.1981.00
66_I130_L1.1971.00
5_R60_A1.1911.00
89_N92_W1.1691.00
117_E120_R1.1591.00
17_A21_A1.1411.00
31_L42_A1.1231.00
102_E105_I1.1161.00
15_L18_A1.1161.00
67_T76_E1.1151.00
9_P54_R1.0991.00
86_W114_R1.0991.00
31_L36_W1.0941.00
9_P13_L1.0921.00
92_W112_H1.0921.00
7_L12_F1.0871.00
43_G47_L1.0841.00
51_W54_R1.0691.00
15_L19_L1.0681.00
18_A22_V1.0671.00
59_H62_D1.0651.00
30_F38_V1.0641.00
69_T72_E1.0641.00
56_N59_H1.0641.00
73_L109_L1.0451.00
79_S84_R1.0381.00
132_R136_R1.0331.00
65_R78_V1.0311.00
117_E127_R1.0111.00
105_I124_P1.0091.00
100_P103_R1.0071.00
126_E129_S0.9981.00
40_P43_G0.9671.00
16_M46_V0.9601.00
62_D79_S0.9561.00
66_I75_V0.9531.00
95_V134_L0.9461.00
8_S57_Y0.9431.00
77_R120_R0.9291.00
95_V131_A0.9131.00
58_R80_R0.9121.00
26_I42_A0.9071.00
133_A137_A0.9031.00
25_A29_A0.8991.00
41_F45_E0.8841.00
21_A25_A0.8771.00
24_L46_V0.8671.00
29_A32_L0.8411.00
23_S27_G0.8411.00
30_F34_G0.8381.00
23_S46_V0.8341.00
129_S132_R0.8331.00
133_A136_R0.8291.00
54_R58_R0.8271.00
96_E108_T0.8211.00
88_F111_S0.8101.00
88_F116_V0.8081.00
64_E120_R0.8081.00
16_M53_F0.7921.00
16_M20_A0.7911.00
27_G42_A0.7841.00
127_R131_A0.7841.00
19_L26_I0.7831.00
79_S83_V0.7731.00
24_L27_G0.7601.00
56_N60_A0.7601.00
28_L31_L0.7591.00
69_T90_P0.7571.00
89_N112_H0.7541.00
92_W113_G0.7511.00
75_V109_L0.7471.00
98_E108_T0.7421.00
49_L52_A0.7411.00
73_L93_V0.7381.00
95_V135_R0.7371.00
10_R13_L0.7361.00
13_L20_A0.7321.00
69_T74_R0.7281.00
19_L23_S0.7271.00
27_G39_L0.7221.00
48_L51_W0.7191.00
29_A43_G0.7061.00
57_Y60_A0.6951.00
125_E128_R0.6901.00
20_A24_L0.6891.00
98_E106_R0.6871.00
24_L28_L0.6871.00
9_P12_F0.6851.00
2_R63_R0.6741.00
71_D91_Y0.6681.00
99_E128_R0.6671.00
72_E89_N0.6651.00
101_E104_G0.6631.00
97_L128_R0.6611.00
97_L107_L0.6551.00
111_S114_R0.6511.00
100_P104_G0.6501.00
54_R57_Y0.6491.00
128_R132_R0.6461.00
71_D89_N0.6461.00
5_R57_Y0.6461.00
50_Y57_Y0.6441.00
12_F53_F0.6391.00
98_E115_R0.6351.00
78_V82_R0.6341.00
34_G37_P0.6310.99
20_A23_S0.6300.99
12_F50_Y0.6300.99
118_I130_L0.6280.99
22_V26_I0.6250.99
99_E105_I0.6180.99
39_L42_A0.6180.99
75_V118_I0.6110.99
5_R11_G0.6070.99
92_W111_S0.5980.99
38_V45_E0.5970.99
106_R120_R0.5920.99
19_L22_V0.5840.99
101_E105_I0.5810.99
23_S45_E0.5810.99
19_L46_V0.5780.99
68_L134_L0.5770.99
52_A55_L0.5750.99
26_I29_A0.5690.99
89_N111_S0.5660.99
37_P43_G0.5650.99
62_D77_R0.5600.99
26_I39_L0.5550.99
97_L131_A0.5520.99
68_L90_P0.5510.99
109_L118_I0.5500.99
122_L126_E0.5490.99
26_I31_L0.5470.99
99_E104_G0.5390.98
103_R106_R0.5320.98
77_R121_F0.5300.98
53_F56_N0.5300.98
23_S26_I0.5270.98
27_G34_G0.5260.98
40_P44_L0.5200.98
119_G130_L0.5180.98
93_V112_H0.5180.98
53_F57_Y0.5160.98
62_D121_F0.5140.98
128_R136_R0.5110.98
107_L119_G0.5030.98
30_F35_A0.5030.98
9_P57_Y0.5010.97
107_L130_L0.5010.97
105_I108_T0.5000.97
62_D80_R0.5000.97
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3jtzA 2 0.5072 11.8 0.916 Contact Map
2m67A 1 0.5217 9 0.92 Contact Map
3ju0A 1 0.5072 8.6 0.921 Contact Map
3d9sA 3 0.442 7.1 0.924 Contact Map
1ud0A 4 0.3116 6.3 0.926 Contact Map
2b6oA 6 0.413 6.3 0.926 Contact Map
1j4nA 4 0.413 6.1 0.926 Contact Map
5a63B 1 0.5145 5.5 0.928 Contact Map
3sjdD 1 0.1159 5.5 0.928 Contact Map
4wd8A 3 0.8478 5.2 0.929 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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